• Journal of Korean Society of Transportation
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• v.20 no.3
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• pp.105-113
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• 2002

As the number of pedestrian accident increases, the reconstruction of an accident becomes important to find the source of the fault. Generally, accidents are reconstructed by the intuition of experts or primitive physics. A reconstruction method is proposed using sophisticated optimization technology. At first, a dynamic simulation model is established for the accident environment. Occupant analysis for automobile crashworthiness is employed. The situation before an accident is identified by optimization. The impact velocity and the position of the pedestrian are utilized as design variables. The design variables are found by minimizing the difference between the simulation and the real accident. The optimization process is performed by linking an occupant analysis program MADYMO to an optimization program VisualDOC. Since the involved analysis is dynamics and highly nonlinear, response surface method is selected for the optimization process. Problems are solved for various situations.

• The Korean Journal of Applied Statistics
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• v.24 no.2
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• pp.383-391
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• 2011

This paper presents a modified entropy-based test of fit for the inverse Gaussian distribution. The test is based on the entropy difference of the unknown data-generating distribution and the inverse Gaussian distribution. The entropy difference estimator used as the test statistic is obtained by employing Vasicek's sample entropy as an entropy estimator for the data-generating distribution and the uniformly minimum variance unbiased estimator as an entropy estimator for the inverse Gaussian distribution. The critical values of the test statistic empirically determined are provided in a tabular form. Monte Carlo simulations are performed to compare the proposed test with the previous entropy-based test in terms of power.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.23 no.2
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• pp.83-87
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• 1978

Using the Buhler laboratory mill three Korean wheat varieties were tested for milling quality. Comparison was made to USA soft white wheat varieties grown in 1977. This test indicated that the milling rate and milling score of Korean varieties were inferior to the variety Paha but were equal to or better than the variety Nugaines.

• Laboratory Medicine Online
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• v.7 no.1
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• pp.13-19
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• 2017

Background: We evaluated a sensitive and quantitative method utilizing fragment analysis of the fms-like tyrosine kinase 3-internal tandem duplication (FLT3-ITD), simultaneously measuring mutant allele burden and length, and verified the analytical performance. Methods: The number and allelic burden of FLT3-ITD mutations was determined by fragment analysis. Serial mixtures of mutant and wild-type plasmid DNA were used to calculate the limit of detection of fragment analysis, conventional PCR, and Sanger sequencing. Specificity was evaluated using DNA samples derived from 50 normal donors. Results of fragment analysis were compared to those of conventional PCR, using 481 AML specimens. Results: Defined mixtures were consistently and accurately identified by fragment analysis at a 5% relative concentration of mutant to wild-type, and at 10% and 20% ratios by conventional PCR and direct sequencing, respectively. No false positivity was identified. Among 481 AML specimens, 40.1% (193/481) had FLT3-ITD mutations. The mutant allele burden (1.7-94.1%; median, 28.2%) and repeated length of the mutation (14-153 bp; median, 49 bp) were variable. The concordance rate between fragment analysis and conventional PCR was 97.7% (470/481). Fragment analysis was more sensitive than conventional PCR and detected 11 additional cases: seven had mutations below 10%, three cases represented conventional PCR failure, and one case showed false negativity because of short ITD length (14 bp). Conclusions: The new fragment analysis method proved to be sensitive and reliable for the detection and monitoring of FLT3-ITD in patients with AML. This could be used to simultaneously assess ITD mutant allele burden and length.

• Korean Journal of Plant Resources
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• v.22 no.1
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• pp.1-4
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• 2009

This study was carried out to evaluate ecological growth characteristics of forty castor bean collections for biodiesel utilization. The emergence date and it's rate of collections was 15-16 days and 82.5%, respectively. It took 63-68 days from emergence to flowering. Stem length was ranged from 173 to 251 cm with an average 211.8 cm. The average stem diameter was 24.2 cm, and showed the high occurrence as 52.5% at the 20.4-24.9 cm. The node number was widely distributed as 13-22 and the average node number was 18.9. The seed numbers were widely distributed as 7-33ea, and the average liter weight of seed was 502.9 g of which minimum weight was 432.3 g and maximum weight was 572.5 g, and the gap between maximum and minimum was 140.2 g. It showed high difference. The 100 grain weight was distributed as 20.3 g-38.3 g which showed high differences between small grain and large grain collections. The seed numbers per plant were varied as 72 at the minimum and 1,011 at the maximum number, and average seed number was 465.2. The total yields of seed per plant were varied as 20 g-266.2 g, and the average yields were 128.1 g.

• Journal of Korean Society of Forest Science
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• v.103 no.4
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• pp.519-527
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• 2014

Deutzia paniculata is an endemic species, which is geographically restricted within southern part of Korea. Four populations of D. paniculata were sampled across its natural range, from the smallest population, Mt. Dalum, which held less than 100 individuals, to the largest, Mt. Unmum, over 3,500 individuals. Artificial pollination study showed that D. paniculata had an obligate outcross breeding system. Major pollinators were two bee species, Lasioglossum exiliceps and Allograpta balteata (de Geer). The breeding system and patterns of allozyme variation of D. paniculata were investigated to understand the population biology and to explain on reserve designs and management proposals relevant to this species. D. paniculata held relatively low genetic variation at the eight allozyme loci surveyed. Measures of genetic variation in this species alleles per locus ($A_s=1.33$), proportion of polymorphic loci (P=23.85%), and expected heterozygosity ($H_{es}=0.110$) were similar to values reported for endemic species. Mt. Dalum population (DAL) was composed with one clone based on allozyme data. Individuals of D. paniculata were frequently included in root connected clusters. Population genetic structure between and within four populations was probably the result of shrinking effective population size and the extinctions of intervening populations. For the conservation of genetic diversity, maximum number of different genotype need to be protected based on genetic structure and mating system.

• Food Science of Animal Resources
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• v.24 no.1
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• pp.8-14
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• 2004

Identification of animals has been made with an ear tag with dummy code, and blood typing has been used for paternity and individual identification in live animals. As various genetic markers are for different cattle breeds vary, the discrete genetic markers are necessary to identify Hanwoo. A total of 740 progeny testing Hanwoo were used to identify Hanwoo specific markers. To examine traceability of individuals by using breed specific genetic codes, four animal were randomly sampled, and traced from live animals to post-slaughter processing stages. The candidate genetic makers used in the study were 16 DNA microsatellites which were identified in romosomes 1 and 14. The number of alleles of those DNA microsatellites ranged from a minimum of 3 to maximum of 12. The heterozygote frequency ranged from 0.022 to 0.824. Effective number of alleles for each DNA microsatellites were 3 to 6. Six selected candidate genetic markers were able ti trace individual cattle with an 100% confidence level.

• Horticultural Science & Technology
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• v.31 no.5
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• pp.580-589
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• 2013

This study was carried out to evaluate the suitability of microsatellite markers for varietal identification and genetic relationship of 170 commercial pepper cultivars. The relationship between marker genotypes and 11 pepper cultivars with different morphological traits was also analyzed. Of the 302 pairs of microsatellite primers screened against 11 pepper cultivars, 24 pairs were highly polymorphic in terms of number of alleles. These markers were applied for the construction of DNA profile data base for 170 commercial pepper cultivars. A total of 164 polymorphic amplified fragments were obtained from 24 microsatellite primers. The average polymorphism information content was 0.673 ranging from 0.324 to 0.824. One hundred and sixty four microsatellite alleles were used to calculate Jaccard's distance coefficients using unweighted pair group method. A clustering group of varieties, based on the results of microsatellite analysis, were categorized into 3 major groups corresponding to morphological traits. The phenogram discriminated all varieties by markers genotypes. These microsatellite markers will be useful as a tool for protection of plant breeders' intellectual property rights through variety identification in distinctness, uniformity and stability test.

• Korean Journal of Breeding Science
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• v.43 no.5
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• pp.405-412
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• 2011

Knowledge of genetic diversity and genetic relationships among inbred lines gives a significant impact on the selection of parental lines for hybrid maize varieties. Genetic diversity and genetic relationships among 86 popcorn inbred lines were analyzed using 50 SSR markers distributed over the whole genome. A total of 256 alleles were identified at all the SSR loci with an average of 5.1 and a range between two and sixteen per locus. The gene diversity values varied from 0.21 to 0.831 with an average of 0.579. The cluster tree generated using the described SSR markers recognized three major groups at 35.8% genetic similarity. Groups I, II, III respectively included 40, 39 and 7 inbred lines. The present study indicates that the SSR markers chosen for this analysis are effective for the assessment of genetic diversity and genetic relationships among 86 popcorn inbred lines in Korea.

• Journal of Animal Science and Technology
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• v.45 no.4
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• pp.523-528
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• 2003

This study was performed to investigate a possible association of the porcine estrogen receptor(ER) locus with the total number of born(TNB) and number of born alive(NBA) in Yorkshire pigs. Using DNAs extracted from 242 Yorkshire pigs, the ER genotype was determined by PvuII PCR-RFLP. The ER allele frequencies of two types of A and B were 0.39 and 0.61, respectively. The least squares means of the litter size by ER genotype was evaluated. The TNB and NBA were found to be associated with an specific ER allele. The genotype at the porcine ER locus has an application potential for marker-assisted selection for litter size in pigs.