• Title/Summary/Keyword: 최강엽

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미역의 부위별 중성 및 극성 지방질의 지방산 조성

  • 최선남;최강주
    • Proceedings of the Korean Journal of Food and Nutrition Conference
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    • 2000.12a
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    • pp.54.1-54
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    • 2000
  • 한국 남해안 완도지역에 자생하는 미역 (Undaria pinnatifida)의 부위별 지방산조성 특성을 계통적으로 분석코자 미역의 부위별 지방질 및 지방산 조성을 silicic acid 컬럼과 기체-액체 크로마토그래피 분석하였다. 총지방질 함량을 비교해 볼 때 엽상부 3.37%, 포자엽 4.59% 및 줄기 2.50%였으나, 엽상부는 총 포리엔산과 linolenic acid(18:3, n-3), stearidonic acid(18:4, n-3), eicosapentaenoic acid(20:5, n-3) 및 docosahexaenoic acid(22:6, n-3)와 같은 n-3계열의 포리엔산의 조성비율이 높았다. 즉 총 포리엔산과 n-3계열의 포리엔산 조성비율을 볼 때 엽상부는 44.18% 및 29.69%였고, 포자엽은 23.63% 및 5.58% 줄기는 26.66% 및 7.37%였다. 한편 중성지방질, 당지방질 및 인지방질의 n-3 포리엔산의 함량을 볼 때 엽상부는 22.40%, 35.82% 및 3.53%로 다른 부위에 비하여 현저하게 높았으나, 줄기는 5.26%, 9.51% 및 2.37%였고 포자엽은 4.49%, 8.01% 및 2.14%로 가장 낮았다.

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Identification of Saponin and Sapogenin in Root, Leaf and Stem of Ginseng by Thin Layer Chromatography (얇은막 크로마토그래피에 의한 인삼(人蔘)의 근(根) 엽(葉) 및 경(莖)의 saponin 및 sapogenin화합물(化合物) 동정(同定))

  • Choi, Kang-Ju;Kim, Seok-Chang;Kim, Man-Wook;Nam, Ki-Yeul
    • Applied Biological Chemistry
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    • v.30 no.4
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    • pp.340-344
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    • 1987
  • Saponins of ginseng root, leaf and stem were identified by TLC. Eleven unknown spots were detected in ginseng leaf and ten unknown spots in ginseng stem on TLC besides seven ginsenosides such as $ginsenoside-Rg_1,\;-Rf,\;-Re,\;-Rd,\;-Rc,\;-Rb_2,\;and\;-Rb_1$ which are contained in ginseng root. $Ginsenoside-Rg_3\;and\;-Rg_2$ were identified on TLC from mild hydrolysates with 50% acetic acid of total saponins from ginseng root, leaf and stem. Meanwhile, panaxadiol, panaxatriol and oleanolic acid were identified from hydrolysates with 7% ethanolic sulfuric acid of total saponin of ginseng root, while panaxadiol and panaxatriol from those of total saponins of ginseng leaf and stem.

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Fatty Acid Compositions of Nutural Lipids and Polar Lipids in the Parts of Miyeok (Undaria pinnatifida) (미역의 부위별 중성 및 극성 지방질의 지방산 조성)

  • 최선남;최강주
    • The Korean Journal of Food And Nutrition
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    • v.13 no.6
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    • pp.553-557
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    • 2000
  • Fatty acid composition of frond, spororphyll and stipe of Miyeok (Undaria pinnatifida) were extensively analyzed to evaluate a nutrient significance of n-3 polyenes, n-6 polyenes, ${\gamma}$-linolenic acid (18:3, n-6) and $\alpha$-linolenic acid (18:3, n-3). Polyenes of $\alpha$-linolenic acid (22:6, n-3), stearidonic acid (18:4, n-3), eicosapentaenoic acid (20:5, n-3) and docosahexaenoic acid (22:6, n-3) were as high as 29.69%, but ${\gamma}$-linolenic acid (18:3, n-6) was as low as 0.90% when compared to total polyenes of 44.18% in frond. The n-3 polyenes of neutural lipids, glycolipids and phospholipids were 22.40%, 35.82% and 3.53% in frond, 5.26%, 9.51% and 2.37% in stipe and 8.01%, 4.49% and 2.14% in sporophyll, respectively. These results suggest that total polyenes and n-3 polyenes in frond of Miyeok may be used as resource of functional food.

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Content Comparison of Proximate Compositions, Various Solvent Extracts and Saponins in Root, Leaf and Stem of Panax Ginseng (인삼의 근, 엽 및 경의 일반성분, 용매별 엑기스 및 사포닌 함량 비교)

  • 김석창;최강주
    • Journal of Ginseng Research
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    • v.11 no.2
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    • pp.118-122
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    • 1987
  • This study was carried out to investigate the effective components, especially saponins, in aerial parts of Panax ginseng. The contents of methanol and ethanol extracts in ginseng leaf were 35.9% and 27.3%, much higher than 15.4% and 8.37% in ginseng root and 21.7% and 16.3% in ginseng stem. And ginseng stem showed as high content of crude fiber as 39.2% which is very high compared with other two parts of ginseng. The contents of total crude saponin were 4.78%, 2.38% and 19.58% in ginseng root, stem and leaf, respectively. In ginseng leaf seven root ginseno-sides-ginsenoside-Rgl(3.32%), -Re(3.24%), -Rd(2.32 %), -Rc(0.65%), -Rb2(0.92%), -Rbl(0.29%), and -Rf(0.11%)-were analyzed by HPLC, Seven gisneno- sides-ginsenoside-Rgl(0.28%), -Re(0.3%), -Rd(0.05%), -Rf(0.01%), -Rc(trace), -Rb2(trace) and -Rbl(trace)-were detected in ginseng stem. Ginseng leaf contained high percentage of saponin and especially of ginsenoside-Rgl, -Re and -Rd. Therefore, ginseng leaf was good resources for ginsenoside-Rgl, -Re and -Rd.

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Purifying Effects of Fat-soluble Solvents in Extracting Saponins from Ginseng Root, Leaf and Stem (인삼(人蔘)의 근(根), 엽(葉) 및 경(莖)의 사포닌 추출과정중(抽出過程中) 지용성(脂溶性) 용매류(溶媒類)의 정제효과(精製效果))

  • Kim, Seok-Chang;Choi, Kang-Ju;Ko, Sung-Ryong;Joo, Hyun-Kyu
    • Applied Biological Chemistry
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    • v.30 no.4
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    • pp.335-339
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    • 1987
  • This study was carried out to investigate effects of fat-soluble solvents on the purification against nan-saponin substances such as chlorophylls and other pigments and on the yields of saponins in separating saponins from ginseng root, leaf and stem. Ginseng root saponins were effectively purified by various fat-soluble solvents while ginseng leaf stem saponins were by chloroform. And alternative extractions of ethyl acetate, ethyl ether, chloroform and benzene there more effective for ginseng leaf stem saponins than that by any single solvent. Contents of crude saponin fractions and total ginsenosides in ginseng leaf were $18.5{\sim}19.5%\;and\;10.8{\sim}11.4%$, which were very high compared with $4.6{\sim}5.1%\;and\;2.0{\sim}2.6%$ in ginseng root or $2.2{\sim}2.5%\;and\;0.63{\sim}0.67%$ in ginseng stem. Therefore, ginseng leaf is good resources for total saponin or $ginsenosides-Rg_1,\;.Re,\;-Rc,\;-Rd,\;-Rb_2\;and\;-Rf$.

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Fatty Acid Compositions of the Various Parts of Ginseng Plant (인삼(人蔘)의 부위별(部位別) 지방산(脂肪酸) 조성(組成))

  • Choi, Kang Ju;Kim, Man Wook;Kim, Dong Hoon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.12 no.4
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    • pp.357-363
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    • 1983
  • An attempt was made to investigate the lipid contents and fatty acid compositions of the roots (rhizome, epidermis, pith, cortex, branch root, fine root) and aerial parts (seed, flesh of seed, leaf, stem) of ginseng plant. Total contents of free and bound lipids in nine parts ranged 0.91 to 3.48%, those of the seeds were 15.08%. Fourteen even-numbered and 4 odd-numbered fatty acids were identified and quantified by GLC. The major fatty acids in each part were linoleic, palmitic, oleic, and linolenic acid. Fatty acid composition of different parts was varied significantly. Fatty acid composition of ginseng seeds was notably different from those of other parts in plant; the amount of oleic and linoleic acids (51.21 and 37.46%) were higher than those of the other parts. The unsaturated fatty acid content of the free lipid in seed, pith, and cortex were higher than those of the other parts in plant.

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Morphological characteristics and RAPD analysis of Epimedium spp. (국내외 수집 삼지구엽초의 형태적 특성 및 유연관계 분석)

  • Lim, Jung-Dae;Seong, Eun-Soo;Choi, Kwang-Joon;Kim, Seung-Kyung;Chung, Ill-Min;Heo, Kweon;Yu, Chang-Yeon
    • Korean Journal of Medicinal Crop Science
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    • v.8 no.2
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    • pp.102-108
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    • 2000
  • Morphological characteristics of nine Epimedium spp was analyzed on the basis of six morphological characters. To analyse the genetic relationship among Epimedium spp., polymerase chain reaction (PCR) was performed with total genomic DNA of 17 Epimedium spp. by using random 8 primers. The genetic diversity and genetic distance among nine Korean collections, seven Japanese and one China collection were used to generate a dendrogram showing phylogenie relationship. Seventeen Epimedium spp were classified into two groups of group I and II, since they were divided into two major groups at the similarity coefficients value of 0.65. In addition, one of the two group, group I was divided into three sub-groups including Epimedium koreanum, Chul-won collections 1, 2, and 3, Yanggu, Hongchon, Hwachun, Chunchon, China, Maehwa (Japanese), E. diphyllum and E. violaceum (Japanese), while group II included Chulwon collection 4 and 5 and Japanese collection. The samples collected at Chulwon district showed close similarity with Japanese collection. Similarity indexes between collection and genetic relationship were related at the levels ranging from 0.6 to 0.9.

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Effect of Artificial Shade Treatment on the Growth and Biomass Production of Several Deciduous Tree Species (인공피음처리가 주요 활엽수종의 생장과 물질생산에 미치는 영향)

  • 최정호;권기원;정진철
    • Journal of Korea Foresty Energy
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    • v.21 no.1
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    • pp.65-75
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    • 2002
  • The study was carried out to determine the growth and biomass production of major deciduous trees including Betula platyphylla var. japonica, Betula schmidtii, Zelkova serrata, Acer mono, Prunes sargentii, and Ligustrum obtusifolium subjected to artificial shade treatment in nursery field. The six deciduous trees seedlings grow for 2 years under different light intensity of 100%, 38-62%, 22-28%, 7-20%, and 2-6% of the full sun light intensity. The results were as follows; In the seedling heights and root collar diameters of shade intolerant species like Betula platyphylla var. japonica and Betula schmidtii, the relative growth rates of seedlings grown in full sun showed 2 times as compared with those subjected to the shade treatment of 2-6% light intensities of full sun. In the shade tolerant species like Acer mono ant Ligustrum obtusifolium, the growth performances were better in the seedlings grown in 38-62% light intensities of full sun. Total dry mass including the dry mass of leaves, shoot and root were as a whole decreased with shade treatment. The ratio of the dry mass of leaves and stem increased the dry mass of root. T/R ratio of the seedlings increased by decreasing the relative light intensity. And the T/R ratio of 2-6% light intensities of full sun was ranged from 1.1~5.0 were greater in the full sun light was ranged from 0.6~3.2. Light intensity by artificial shade treatment decreased in deciduous trees when compared on the whole, it showed tendency that SLA increases, increased that seeing resemblant tendency in LAR and LWR and changed of light intensity is strong, it increased that showed difference as statistical. But, LWR of Betula platyphylla var. japonica increased gradually and showed tendency that decreases rapidly in the shade treatment of 2-6% light intensities of full sun. This result is thought that biomass production decreased by shading treatment influenced in physiological characteristics such as leaf area and decrease of the leaf amount.

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