• Journal of Life Science
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• v.19 no.7
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• pp.845-851
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• 2009

A vector harboring double cassettes; a heterologous gene expression cassette of pHCE-InaN-antigen and a ghost formation cassette of pAPR-cI-E lysis 37 SDM was constructed and introduced to E. coli DH5a. For the production of a bacterial ghost vaccine, bacterial ghosts from E. coli / Streptococcus iniae with four different types of antigens - enolase, GAPDH, sagA and piaA - were produced by the optimization of fermentation parameters such as a glucose concentration of 1 g/l, agitation of 300 rpm and aeration of 1 vvm. Efficiency of ghost bacteria formation was evaluated with cultures of OD$_{600}$=1.0, 2.0 and 3.0. The efficiency of the ghost bacteria formation was 99.54, 99.67, 99.99 and 99.99% with inductions at OD$_{600}$=3.0, 1.0, 2.0 and 1.0 for E. coli/S. iniae antigens enolase, piaA, GAPDH and sagA, respectively. Ghost bacteria as a vaccine was harvested by centrifugation. The antigen protein expressions were analyzed by SDS-PAGE and western blot analysis, and the molecular weights of the enolase, piaA, GAPDH and sagA were 78, 26, 67 and 26 kDa, respectively. The molecular weights of the expressed antigens were consistent with theoretical sizes obtained from the amino acid sequences.

• School Mathematics
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• v.16 no.4
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• pp.803-825
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• 2014

From the early stages of learning algebra, literal symbols are used to represent algebraic objects such as variables and parameters. The concept of parameters contains both indeterminacy and fixity resulting in confusion and errors in understanding. The purpose of this research is to compare the beginners of algebra and pre-service teachers who completed secondary mathematics education in terms of understanding this paradoxical nature of parameters. We recruited 35 middle school students in eight grade and 73 pre-service teachers enrolled in a undergraduate course at one university. Using them we conducted a survey on the perception of the nature of parameters asking if one considers parameters suggested in a problem as variables or constants. We analyzed the collected data using the mixed method of qualitative and quantitative approaches. From the analysis results, we identified several difficulties in understanding of parameters from both groups. Especially, our statistical analysis revealed that the proportions of subjects with limited understanding of the concept of parameters do not differ much in two groups. This suggests that learning algebra in secondary mathematics education does not improve the understanding of the nature of parameters significantly.

• Journal of Animal Science and Technology
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• v.50 no.3
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• pp.429-436
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• 2008

A bacterium producing cold-active cellulase and xylanase was isolated from pig feces. The isolate, DK42 strain, was found to be the Gram-positive, non-motile, catalase-positive, and spore-forming stain. Under an electron microscope, the cells were observed to be rod-shaped. The isolate was identified as Bacillus licheniformis DK42 on the basis of morphological and biochemical properties as well as 16S rRNA gene sequences. The characterization of crude cellulase and xylanase from B. licheniformis DK42 was investigated. Cellulase exhibited an optimum temperature and pH at 45℃ and 6.0, whereas xylanase exhibited an optimum temperature and pH at 55℃ and 6.0. Especially cellulase maintained approx. 50% of its maximum activity even at 10℃, indicating that it is cold-active. Both cellulase and xylanase were stable after 2hr at 35℃, whereas they lost their activities after 30min at 65℃.

• Journal of the Korean Institute of Gas
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• v.25 no.4
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• pp.10-18
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• 2021

CO₂ storage technology in an aquifer is one of the most effective way to decrease global warming due to a high storage capacity and economics. A demonstration-scale offshore CO₂ storage project was performed in a geological deep aquifer in the Pohang Basin, Korea for a technological development of large-scale CO₂ storage. A challenging issue in the early design stage of the project was to establish the proper injectivity during CO₂ injection. To solve this issue, injection conditions were calculated by calculating injection rate, pressure, temperature, CO₂ phase change, and thermodynamic properties. For this study, we simulated and numerically analyzed CO₂ phase change from gas to supercritical phase and flow behavior in transport piping and injection tubing using OLGA program. Our results provide the injectivity conditions of CO₂ injection system combined with a bottomhole pressure of an aquifer.

• Journal of the Korean Geosynthetics Society
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• v.21 no.1
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• pp.11-21
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• 2022

In this study, the individual vacuum seepage consolidation method, a new soft ground improvement method, was developed to supplement the conventional suction drain method (individual vacuum preloading method) and the geotechnical behavior was predicted through numerical analysis. If the individual vacuum seepage consolidation method applied, the effect of accelerating settlement and increasing the amount of settlement was high when the aquifer was located in the middle or at the bottom of the layer to the target improvement layer. It was found that the pumping amount in the aquifer does not affect the settlement behavior when it exceeds a certain level. Even vacuum pumping wells were installed in various locations, such as inside or outside of the embankment, the difference in settlement and horizontal displacement was insignificant. In addition, it was predicted that the settlement rate was the fastest and the horizontal displacement (inward) was large when both methods were carried out at the same time. Since this method can reach the target settlement amount very quickly, it was confirmed that it is possible to increase the spacing of vertical drain, thereby securing economic feasibility.

• The Journal of Engineering Geology
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• v.28 no.3
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• pp.443-453
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• 2018

Radial collector wells have been widely used for large-capacity groundwater development in riparian environments, and many empirical equations have been developed for initial estimates of groundwater yield. We compare the initial yield estimates produced by several empirical equations for the radial collector well at the Anseongcheon stream site. The results of Babac's, Kordas', and Petrovic's methods are similar to the actual yield of $6,124m^3/d$, but Milojevic's method predicts a higher yield than the observed value. The conditions under which these methods are applicable explain the observed differences between the actual and estimated yields. The applicability of empirical equations is evaluated by changing the input variables of distance from well to river, number of horizontal wells, thickness of aquifer, length of horizontal well, and hydraulic conductivity. The results indicate that the conditions under which each method is applicable must be considered carefully when estimating groundwater yield, and hydraulic conductivity must be estimated accurately.

• Microbiology and Biotechnology Letters
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• v.16 no.3
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• pp.187-192
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• 1988

Production of 4-androstene-3,17-dione(AD) from cholesterol by microbial conversion was investigated. To facilitate the solubilization of cholesterol in the fermentation broth, ethanol was used as an organic solvent. Inhibition on cell growth by ethanol was observed to be negligible upto 2% (V/V) concentration. Microbial conversion was successfully carried out with high yield when the cholesterol was added at early logarithmic growth phase with pH control at 7.0. In order to improve the process productivity, bioconversion was conducted at various mode of cholesterol addition ; 0.1% (V/W) of cholesterol was found to be most appropriate for solubilization in ethanol and was added intermittently. When added three time(total 3 g/$\ell$), overall bioconversion yield reached upto 65% while single addition of same amount of cholesterol (3 g/$\ell$) yielded about 40% conversion.

• Transactions of the Korean Society of Mechanical Engineers
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• v.12 no.4
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• pp.642-651
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• 1988

The present paper develops finite element procedures to calculate displacements, strains and stresses in initially stressed elastic solids subjected to static or time-dependent loading conditions. As a point of departure, we employ Hamilton's principle to obtain nonlinear equations of motion characterizing the displacement in a solid. The equations of motion reduce to linear equations of motion if incremental stresses are assumed to be infinitesimal. In the case of linear problem, finite element solutions are obtained by Newmark's direct integration method and by modal analysis. An analytic solution is referred to compare with the linear finite element solution. In the case of nonlinear problem, finite element solutions are obtained by Newton-Raphson iteration method and compared with the linear solution. Finally, the effect of the order of Gauss-Legendre numerical integration on the nonlinear finite element solution, has been investigated.

• Water for future
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• v.27 no.1
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• pp.79-88
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• 1994

This study was conducted to analyse groundwater flow in the Bugok hot spring area using the MODFLOW model which can simulate three dimensional groundwater flow both in confined and unconfined aquifers. Based on this study the following conclusions were obtained: 1) The hydraulic conductivity and the specific storage of the aquifer were 0.0135 m/day and 0.020, respectively, and the model-predicted groundwater elevation agreed well with the observed one. 2) Simulation results showed that the groundwater level declines at the end of the one-year simulation period when the annual recharge rate is small and the annual pumping rate high, which is the worst combination. Except that combination, the groundwater level does not decline at the end of one-year simulation period indication the pumping rates used were allowable. 3) The safe yield depends upon the magnitudes of the recharge and pumping rates. The pumping rate should not produce excess decline of groundwater level around April when the water level is the lowest in a year.

• Korean Journal of Optics and Photonics
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• v.18 no.1
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• pp.19-23
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• 2007

We derived analytically the generalized curvature linear equation useful in the initial optical design of a two-mirror system with finite object distance, including an infinite object distance from paraxial ray tracing and Seidel third order aberration theory for coma coefficient. These aberration coefficients for finite object distance were described by the curvature, the inter-mirror distance, and the effective focal length. The analytical equations were solved by using a computer with a numerical analysis method. Two useful linear relationships, determined by the generalized curvature linear equations relating the curvatures of the two mirrors, for the cancellation of each aberration were shown in the numerical solutions satisfying the nearly zero condition ($<10^{-10}$) for each aberration coefficient. These equations can be utilized easily and efficiently at the step of initial optical design of a two-mirror system with finite object distance.