• Microbiology and Biotechnology Letters
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• v.15 no.6
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• pp.396-401
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• 1987

Protein productivities of a cloned gene ($\beta$-galactosidase) and the cultural performances of two recombinant Escherichia coli strains, which use different host-vector systems, were studied. E. coli JM109/pTBG10 strain which carries Tac promoter had higher protein productivity than E. coli MH3000 (pRKc1857)/pASI(lacZ) strain which carries pL promoter. Induction of protein syn-thesis was optimum at the initial-and mid-logarithmic growth phases for both strains. Oxygen demand was observed to be very high during the cloned gene expression, and could be alleviated to some extent through pH control. The ratio of specific growth rates of plasmid-harboring to plasmidfree cell, $\mu$+ /$\mu$-, of the high productivity strain was observed to be lower than that of the low productivity one. Plasmid stability was analyzed for 20-30 generations, and it was found that the traction of plasmid-harboring cells dropped to l0% level in about 25 generations for both strains when the cloned gene expression was induced.

• 한국신재생에너지학회:학술대회논문집
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• 2009.11a
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• pp.571-571
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• 2009

지열은 날씨와 기온 등에 영향을 받지 않고 연중 가동할 수 있어 기저부하를 담담할 수 있는 유일한 신재생에너지 자원이므로 이에 대한 기술개발이 시급하다. 우리나라는 비화산지대이며 지중 온도가 가장 높은 지역의 5km에서 약 $170^{\circ}C$ 내외이므로 외국에 비해 지온경사도가 크지 않은 편이다. 그리고 3km 이상에서는 지하대수층이 거의 존재하지 않기 때문에 지열발전을 위해서는 EGS 기법을 도입할 수 밖에 없는 실정이다. 그리고 지열수를 확보할 수 있는 온도범위가 약 $100{\sim}150^{\circ}C$ 정도이므로 이에 적합한 지열발전 플랜트를 선정할 필요가 있다. 일반적으로 지열발전에 적용되는 플랜트는 건증기 지열발전, 플래쉬증기 지열발전, 바이너리 사이클 지열발전으로 분류할 수가 있으나 국내 여건에 맞는 방식으로서 바이너리 사이클 발전으로서 ORC 플랜트 또는 Kalina 사이클 플랜트가 적합하므로 이에 대한 기술 개발이 적극적으로 이루어져야 한다. 따라서 국내 지열발전의 기술개발에 있어서 핵심요소는 심부천공 및 EGS를 위한 인공파쇄기술과 지상 플랜트로서 저온지열 발전 플랜트의 기술확보가 필요한 실정이다. 이와 같은 기술개발이 완성되면 발전 뿐만아니라 집단지역난방, 온실 및 양어장 등에도 열공급이 가능한 열병합발전이 가능하게 될 것이다. 또한, EGS 기술로서 상업적 성공을 이룬 것은 세계적으로 2~3개 사례에 불과한 신기술로서, EGS 기술의 국내 조기 실현으로 기술 선점 및 해외 수출을 모색할 필요가 있다. 그리고 심부 지열자원은 국내 어디에나 부존하는 ubiquitous 자원이며 이산화탄소 배출이 전무한 청정 국산 에너지 자원이나, 이의 개발에는 높은 초기 투자비와 risk를 요하므로 민간 업체의 투자가 제약을 받는다. 따라서 정부의 적극적인 지원하에 산.학.연 중심으로 시범보급이 우선 이루어진 후 민간의 자발적 투자를 통한 지열 개발을 유도할 필요가 있다.

• Journal of Life Science
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• v.9 no.1
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• pp.111-120
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• 1999

A bacteriocin-producing strain, J-105, was isolated from Kimchi and identified as Lactococcus sp. The optimum conditions for the bacteriocin production from the isolated microorganism were evaluated. For maximum yield of bacteriocin from Lactotoccus sp. J-105, the cell should be harvested at the early stationary phase and temperature, pH and NaCl concentration should be $25^{\circ}C$, pH 8.0 and without the addition of NaCl, respectively. Maltose should be used as a carbon source and organic nitrogen such as polypeptone should be used as a nitrogen source for the best yield. The bacteriocin from isolate was inhibitory against Acetobacter aceti, Bacillus subtilis and several strains of lactic acid bacteria. The bacteriocin of J-105 was sensitive to pepsin, but stable for heat treatment. It was stable even at autoclaving temperature for 15 min.

• Journal of Korea Society of Industrial Information Systems
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• v.11 no.1
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• pp.29-37
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• 2006

Due to the spread of mobile communication with the development of computer network, nowadays various types of multimedia data play an important role in many areas such as entertainments, culture contents, e-commerce or medical science. But for the real application of these data, the security in the course of saving or transferring them through the public network should be assured. In this sense, many encryption algorithm have been developed and utilized. Nonetheless, most of them have focused on the text data. So they may not be suitable to the multimedia application because of their large size and real time constraint. In this paper, a chaotic map has been employed to create a symmetric stream type of encryption scheme which may be applied to the digital images with a large amounts of data. Then an efficient algebraic encryption algorithm based on the elementary operations of the Boolean matrix and image data characteristics.

• School Mathematics
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• v.14 no.3
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• pp.275-296
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• 2012

This study investigated elementary school students' understanding of basic functional relationships. It analyzed the written responses from a total of 2087 students of second, fourth, and sixth graders using tests that examined their understanding of five types of functional relationships. The results of this study showed that students tended to be more successful as their grades went up with regard to all the problem types. There were statistically differences among the three grade levels. Even lower graders were quite successful in dealing with additive relation, direct proportion, and inverse proportion. However the items dealing with square relation and linear relation were difficult even to sixth graders. It was common that students were good at completing the table by looking for a pattern from the given numbers but that they had difficulties in anticipating the value of 'y' when the value of 'x' is given either as a big number or as a symbol. Given these results, this paper includes issues and implications on how to foster functional thinking ability at the elementary school.

• Journal of the Korean Chemical Society
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• v.30 no.1
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• pp.3-8
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• 1986

The investigation of the effect of solvent structure on the first-order solvolysis of cis-$[Co(en)_2ClNO_2]^+$ion has been extended to water + co-solvent mixtures where the co-solvents are glycerol, ethylene glycol, isopropyl alcohol and t-butyl alcohol. Rates of solvolysis have been evaluated by spectrophotometric method at temperature 25∼30$^{\circ}$C. The polarity of solvent has influence on the variation of rate constant. The non-linear plot of the rate constant in log scale versus $\frac{D-1}{2D+1}$ implies that change in solvent structure with composition plays an important role in determining the variation of rate constant. The linearity of the plot of the rate constant in log scale versus the Grundwald-Winstein Y factor confirms that the solvolysis is an Id-type process with considerable extension of the metal chloride bond in the transition state. In the Kivinen equation the slope of the plot of log k versus $log(H_2O)$ suggests that the solvolysis is also an Id-type process. The application of free energy cycle shows that the effect of solvent structure is greater in the transition state than in the initial state.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.2
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• pp.160-164
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• 1999

Algicidal activities of Alteromonas sp. SR-14 against Chaetoceros calcitrans were investigated at various culture conditions. The algicidal activity by Alteromonas sp. SK-14 was dependent on temperature. In mixed culture of C, calcitrans and Alteromonas sp. SR-14 at various temperatures, the algicidal activity of Alteronzonas sp. SR-14 was the highest at $20^{\circ}C$, but not showed algicidal activity above $25^{\circ}C$. With the inoculation of $10^4$ cells/ml of C. calcitrans, the diatom could not grow at the microalgal culture condition until 15 days by the simultaneous inoculation of less than 10 cells/ml of Alteromonas sp. SR-14. Alteromonas sp. SR-14 showed the strongest algicidal activity against logarithmic phase cells of C. calcitrans. During the mixed culture of C. calcitrans and Alteromonas sp. SR-14, supplementation of Conwy medium nutrients, changes of light intensity with 1,300$\~$4,600 lux and agitation with 200 rpm did not affect the algicidal activity.

• The Mathematical Education
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• v.50 no.2
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• pp.213-231
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• 2011

The purpose of this study is to analyze the 6th graders' understanding of the concepts of variable on various aspects of school algebra. For this purpose, the test of concepts of variable targeting a sixth-grade class was conducted and then two students were selected for in-depth interview. The level of mathematics achievement of the two students was not significantly different but there were differences between them in terms of understanding about the concepts of variable. The results obtained in this study are as follows: First, the students had little basic understanding of the variables and they had many cognitive difficulties with respect to the variables. Second, the students were familiar with only the symbol '${\Box}$' not the other letters nor symbols. Third, students comprehended the variable as generalizers imperfectly. Fourth, the students' skill of operations between letters was below expectations and there was the student who omitted the mathematical sign in letter expressions including the mathematical sign such as x+3. Fifth, the students lacked the ability to reason the patterns inductively and symbolize them using variables. Sixth, in connection with the variables in functional relationships, the students were more familiar with the potential and discrete variation than practical and continuous variation. On the basis of the results, this study gives several implications related to the early algebra education, especially the teaching methods of variables.

• Journal of Wetlands Research
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• v.23 no.4
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• pp.342-351
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• 2021

The objective of this study is to evaluate the change of groundwater and its effect to hydraulic head or parameter sensitivity in order to predict the effect of a new groundwater well using MODFLOW. With the downstream area of the Jun stream basin in the Donghae city, two condition were simulated; (i) First simulation condition is to simulate natural steady-state and the groundwater heads for observation wells are calibrated. After the calibration, existing wells are simulated to find out the pure effect of the next simulation condition, (ii) Secondly, the design wells are planed and simulated for the solution of water shortage problem at Donghae city. As a result of the simulation, the maximum drawdown which is approximately 0.35m occurs in layer 1. This represents that there is no intrusion of salty water and the design plan is acceptable. Finally, the sensitivity analysis of each parameter in MODFLOW is accomplished. We found out that the sensitive parameters are initial head, anisotropic factor, hydraulic conductivity, and general head boundary

• Journal of the Korean Institute of Gas
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• v.25 no.3
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• pp.16-26
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• 2021

The global warming problem has arose, the supply eco-friendly vehicles such as HFCEVs is increasing around world and Korea is fully supporting subsidies, tax cut to form an initial market for HFCEVs. The key to the safety of HFCEVs is pressure vessels stored hydrogen, and although these pressure vessels must be inspection regularly, the existing inspection stations are insufficient to meet the demand for inspection. Therefore, it is important to establishment of pressure vessels inspection station for safety management of HFCEVs. In this study, it estimates innovation coefficient, imitation coefficient in Bass model by using electric vehicle sales data, and foretasted the supply of HFCEVs by region & the demand for inspection by region using the Bass diffusion model. As a result, the inspection demand for pressure vessels in HFCEVs in 2040 was 690,759 units, and it was confirmed 191 new inspection stations and 1,124 inspectors were needed to prepare for this.