• Proceedings of the Korea Water Resources Association Conference
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• 2008.05a
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• pp.1899-1903
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• 2008

대수층의 저유량이 풍부한 강변여과수 개발 예정지역의 충적층(지표면하 $25{\sim}35\;m$ 구간)에서 수리전도도와 종분산지수의 규모종속효과를 규명하기 위해 양수시험과 수렴흐름 추적자시험이 수행 되었다. 양수시험과 추적자시험의 규모는 2 m 와 5 m 이었으며 양수시험은 5개 공, 추적자시험은 3개 공을 이용하여 수행되었다. 양수시험은 일정한 양수율($2,500\;m^3/day$)로 수행되었으며, 양수 시작 후 경과시간에 따른 수위변화 자료를 AQTESOLV 3.5 프로그램에 입력하여 해석하였다. 시험 대수층의 수리전도도는 양수정에서 $1.745{\times}10^{-3}\;m/sec$, 양수정에서 이격거리가 2 m 구간에서는 $2.161{\times}10^{-3}\;m/sec$와 $2.270{\times}10^{-3}\;m/sec$ 이었으며, 이격거리가 5 m 구간에서는 $2.452{\times}10^{-3}\;m/sec$와 $2.591{\time}10^{-3}m/sec$로 산정되었다. 그리고, 양수정에서 회복시험 시 Theis(Recovery) 방법에 의해 해석된 수리전도도는 $1.603{\times}10^{-3}\;m/sec$이었다. 양수정에서 관측정의 이격거리(d)에 따른 수리전도도(K) 증가함수는 log K=0.0693 log d-2.671와 log K=0.0817 log d-2.655로 추정되었으며, 결정 계수는 각각 0.965와 0.979로서 매우 높게 나타났다. 따라서 양수정에서의 이격거리가 멀수록 수리전도도가 증가하는 규모종속을 확인하였으며, 또한 시험대수층의 수리전도도가 방사상으로 유사하게 분포하고 있음을 알 수 있었다. 수렴흐름 추적자시험의 양수율은 $2,500\;m^3/day$ 이었으며, 2개의 주입정에 염소이온 5 kg을 순간 주입하였다. 염소이온의 농도이력곡선을 작성하여 초기도달시간과 최고농도의 차이를 분석하였으며, 누적질량회수곡선을 통해 양수 후 경과시간에 따른 염소이온의 질량회수율을 분석하였다. 그리고, 염소이온농도 대 누적질량회수율의 이력그래프를 작성하여 누적질량회수율에 따른 염소이온농도의 증가와 감소 변화를 분석하였다. 또한, 염소이온농도의 증가/감소 구간에 대한 선형회귀분석을 수행하여 농도 증가율과 감소율의 변화를 파악하였다. 양수정에서 관측된 경과시간별 염소이온농도 자료를 CATTI 코드의 "Converging Radial Flow With Instantaneous Injection" 해석법에 적용하여 종분산지수를 추정하였다. 양수정에서 이격거리가 2 m인 경우의 종분산지수는 0.4152 m, 이격거리가 5 m인 경우의 종분산지수는 3.2665 m이었다. 따라서 양수정에서 이격거리가 멀수록 종분산지수가 증가하는 규모종속효과를 확인하였으며, 또한 이격거리에 대한 종분산지수의 비는 각각 0.21과 0.65 정도로서 증가하였다.

• Journal of Life Science
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• v.19 no.7
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• pp.845-851
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• 2009

A vector harboring double cassettes; a heterologous gene expression cassette of pHCE-InaN-antigen and a ghost formation cassette of pAPR-cI-E lysis 37 SDM was constructed and introduced to E. coli DH5a. For the production of a bacterial ghost vaccine, bacterial ghosts from E. coli / Streptococcus iniae with four different types of antigens - enolase, GAPDH, sagA and piaA - were produced by the optimization of fermentation parameters such as a glucose concentration of 1 g/l, agitation of 300 rpm and aeration of 1 vvm. Efficiency of ghost bacteria formation was evaluated with cultures of OD$_{600}$=1.0, 2.0 and 3.0. The efficiency of the ghost bacteria formation was 99.54, 99.67, 99.99 and 99.99% with inductions at OD$_{600}$=3.0, 1.0, 2.0 and 1.0 for E. coli/S. iniae antigens enolase, piaA, GAPDH and sagA, respectively. Ghost bacteria as a vaccine was harvested by centrifugation. The antigen protein expressions were analyzed by SDS-PAGE and western blot analysis, and the molecular weights of the enolase, piaA, GAPDH and sagA were 78, 26, 67 and 26 kDa, respectively. The molecular weights of the expressed antigens were consistent with theoretical sizes obtained from the amino acid sequences.

• School Mathematics
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• v.16 no.4
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• pp.803-825
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• 2014

From the early stages of learning algebra, literal symbols are used to represent algebraic objects such as variables and parameters. The concept of parameters contains both indeterminacy and fixity resulting in confusion and errors in understanding. The purpose of this research is to compare the beginners of algebra and pre-service teachers who completed secondary mathematics education in terms of understanding this paradoxical nature of parameters. We recruited 35 middle school students in eight grade and 73 pre-service teachers enrolled in a undergraduate course at one university. Using them we conducted a survey on the perception of the nature of parameters asking if one considers parameters suggested in a problem as variables or constants. We analyzed the collected data using the mixed method of qualitative and quantitative approaches. From the analysis results, we identified several difficulties in understanding of parameters from both groups. Especially, our statistical analysis revealed that the proportions of subjects with limited understanding of the concept of parameters do not differ much in two groups. This suggests that learning algebra in secondary mathematics education does not improve the understanding of the nature of parameters significantly.

• Journal of Animal Science and Technology
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• v.50 no.3
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• pp.429-436
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• 2008

A bacterium producing cold-active cellulase and xylanase was isolated from pig feces. The isolate, DK42 strain, was found to be the Gram-positive, non-motile, catalase-positive, and spore-forming stain. Under an electron microscope, the cells were observed to be rod-shaped. The isolate was identified as Bacillus licheniformis DK42 on the basis of morphological and biochemical properties as well as 16S rRNA gene sequences. The characterization of crude cellulase and xylanase from B. licheniformis DK42 was investigated. Cellulase exhibited an optimum temperature and pH at 45℃ and 6.0, whereas xylanase exhibited an optimum temperature and pH at 55℃ and 6.0. Especially cellulase maintained approx. 50% of its maximum activity even at 10℃, indicating that it is cold-active. Both cellulase and xylanase were stable after 2hr at 35℃, whereas they lost their activities after 30min at 65℃.

• Journal of the Korean Institute of Gas
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• v.25 no.4
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• pp.10-18
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• 2021

CO₂ storage technology in an aquifer is one of the most effective way to decrease global warming due to a high storage capacity and economics. A demonstration-scale offshore CO₂ storage project was performed in a geological deep aquifer in the Pohang Basin, Korea for a technological development of large-scale CO₂ storage. A challenging issue in the early design stage of the project was to establish the proper injectivity during CO₂ injection. To solve this issue, injection conditions were calculated by calculating injection rate, pressure, temperature, CO₂ phase change, and thermodynamic properties. For this study, we simulated and numerically analyzed CO₂ phase change from gas to supercritical phase and flow behavior in transport piping and injection tubing using OLGA program. Our results provide the injectivity conditions of CO₂ injection system combined with a bottomhole pressure of an aquifer.

• Journal of the Korean Geosynthetics Society
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• v.21 no.1
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• pp.11-21
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• 2022

In this study, the individual vacuum seepage consolidation method, a new soft ground improvement method, was developed to supplement the conventional suction drain method (individual vacuum preloading method) and the geotechnical behavior was predicted through numerical analysis. If the individual vacuum seepage consolidation method applied, the effect of accelerating settlement and increasing the amount of settlement was high when the aquifer was located in the middle or at the bottom of the layer to the target improvement layer. It was found that the pumping amount in the aquifer does not affect the settlement behavior when it exceeds a certain level. Even vacuum pumping wells were installed in various locations, such as inside or outside of the embankment, the difference in settlement and horizontal displacement was insignificant. In addition, it was predicted that the settlement rate was the fastest and the horizontal displacement (inward) was large when both methods were carried out at the same time. Since this method can reach the target settlement amount very quickly, it was confirmed that it is possible to increase the spacing of vertical drain, thereby securing economic feasibility.

• The Journal of Engineering Geology
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• v.28 no.3
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• pp.443-453
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• 2018

Radial collector wells have been widely used for large-capacity groundwater development in riparian environments, and many empirical equations have been developed for initial estimates of groundwater yield. We compare the initial yield estimates produced by several empirical equations for the radial collector well at the Anseongcheon stream site. The results of Babac's, Kordas', and Petrovic's methods are similar to the actual yield of $6,124m^3/d$, but Milojevic's method predicts a higher yield than the observed value. The conditions under which these methods are applicable explain the observed differences between the actual and estimated yields. The applicability of empirical equations is evaluated by changing the input variables of distance from well to river, number of horizontal wells, thickness of aquifer, length of horizontal well, and hydraulic conductivity. The results indicate that the conditions under which each method is applicable must be considered carefully when estimating groundwater yield, and hydraulic conductivity must be estimated accurately.

• Microbiology and Biotechnology Letters
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• v.16 no.3
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• pp.187-192
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• 1988

Production of 4-androstene-3,17-dione(AD) from cholesterol by microbial conversion was investigated. To facilitate the solubilization of cholesterol in the fermentation broth, ethanol was used as an organic solvent. Inhibition on cell growth by ethanol was observed to be negligible upto 2% (V/V) concentration. Microbial conversion was successfully carried out with high yield when the cholesterol was added at early logarithmic growth phase with pH control at 7.0. In order to improve the process productivity, bioconversion was conducted at various mode of cholesterol addition ; 0.1% (V/W) of cholesterol was found to be most appropriate for solubilization in ethanol and was added intermittently. When added three time(total 3 g/$\ell$), overall bioconversion yield reached upto 65% while single addition of same amount of cholesterol (3 g/$\ell$) yielded about 40% conversion.

• Transactions of the Korean Society of Mechanical Engineers
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• v.12 no.4
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• pp.642-651
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• 1988

The present paper develops finite element procedures to calculate displacements, strains and stresses in initially stressed elastic solids subjected to static or time-dependent loading conditions. As a point of departure, we employ Hamilton's principle to obtain nonlinear equations of motion characterizing the displacement in a solid. The equations of motion reduce to linear equations of motion if incremental stresses are assumed to be infinitesimal. In the case of linear problem, finite element solutions are obtained by Newmark's direct integration method and by modal analysis. An analytic solution is referred to compare with the linear finite element solution. In the case of nonlinear problem, finite element solutions are obtained by Newton-Raphson iteration method and compared with the linear solution. Finally, the effect of the order of Gauss-Legendre numerical integration on the nonlinear finite element solution, has been investigated.

• Water for future
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• v.27 no.1
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• pp.79-88
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• 1994

This study was conducted to analyse groundwater flow in the Bugok hot spring area using the MODFLOW model which can simulate three dimensional groundwater flow both in confined and unconfined aquifers. Based on this study the following conclusions were obtained: 1) The hydraulic conductivity and the specific storage of the aquifer were 0.0135 m/day and 0.020, respectively, and the model-predicted groundwater elevation agreed well with the observed one. 2) Simulation results showed that the groundwater level declines at the end of the one-year simulation period when the annual recharge rate is small and the annual pumping rate high, which is the worst combination. Except that combination, the groundwater level does not decline at the end of one-year simulation period indication the pumping rates used were allowable. 3) The safe yield depends upon the magnitudes of the recharge and pumping rates. The pumping rate should not produce excess decline of groundwater level around April when the water level is the lowest in a year.