• Journal of the Society of Cosmetic Scientists of Korea
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• v.37 no.3
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• pp.275-282
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• 2011

Novel whitening agents were prepared using peptide-Natural origin compound derivatives. The peptide could be an antagonist of MC1R and Natural origin compound were well-known material as a Tyrosinase inhibitor. We also suggest the new assay method which could evaluate the Antagonistic effectiveness to MC1R using cAMP signaling pathway. 24 candidates were synthesized and 11 peptide derivatives were selected by cAMP assay method. To evaluate cAMP assay, the selected peptide derivatives were assayed to evaluate their melanogensis inhibitory activity. At this work, we could know that the sequences which include -RW- have a melanogensis inhibitory activity, and cAMP assy could use as a evaluating method of MC1R antagonist. But, to evaluate the whitening activity of some material, cross-checking with melanin inhibitory assay method was recommended.

• Proceedings of the Korean Vacuum Society Conference
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• 2012.02a
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• pp.237-237
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• 2012

반도체 소자를 취급하는 반도체 산업은 여러 산업 중에서도 부가가치율이 높은 것의 하나이다. 반도체 공정은 산화막과 질화막은 각각 다양한 두께와 방법으로 제조되고 있으며 CVD, PEALD 이용한 증착 공정을 기반으로 하고 있다. 하지만 양산에서의 많은 문제 요소를 가지고 있다. 첫째, 양적인 실시간과 전구체의 정상상태를 확인 할 수 없으므로 인한 질 적인 저하등을 요소를 가지고 있으며 둘째, 양산 후 남은 전구체를 외관상의 변색, 점도 변화를 통해 변질을 확인하고 전구체를 교체함으로써 엄청난 경제적인 손실과 안정적인 공급에 어려움이 있다. 그러므로 본 연구에서는 reference 전구체와 공정에서 사용된 전구체를 이용하여 Vapor Pressure 측정과 FT-IR (Fourier transform-infrared), QMS을 이용하여 개발된 전구체의 기상 안정성 및 반응성을 실시간으로 진단하여 기존의 전구체와의 차별성을 확인하고 우수한 전구체를 선별하기 위한 연구를 진행하였다. 또한 변화에서의 분자 상태 변형을 진담함으로 인해 기업의 양산의 경제적인 손실을 줄일 수 있을 뿐만 아니라 성장 조건에 따라 전구체 박막 특성을 논의 할 수 있을 것이다.

• Proceedings of the Korean Society of Potoscience Conference
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• spring
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• pp.14-14
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• 1995

• Microbiology and Biotechnology Letters
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• v.39 no.2
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• pp.104-110
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• 2011

Actinomycetes, Gram positive soil bacteria, are valuable microorganisms which produce useful secondary metabolites including antibiotics, antiparasitic substances, anti-cancer drugs, and immunosuppressants. Although a major family of actinomycetes, known as streptomycetes, has been intensively investigated at the molecular level for several decades, a potentially valuable and only recently isolated non-streptomycetes rare actinomycetes (NSRA) family has been poorly characterized due to lack of proper genetic manipulation systems. Here we report that a PCR-based genome screening strategy was performed with approximately 180 independently isolated actinomycetes strains to isolate potentially valuable NSRA strains. Thanks to this simple PCR-based genome screening strategy we were able to identify only seven NSRA strains, followed by 16S rRNA sequencing for confirmation. Through further bioassays, one potentially valuable NSRA strain (tentatively named Nocardiopsis species MMBL010) was identified which possessed both antifungal and antibacterial activities, along with the presence of polyketide synthase and non-ribosomal peptide synthase genes. Moreover, Nocardiopsis species MMBL010, which was intrinsically recalcitrant to genetic manipulation, was successfully transformed via E. coli-driven conjugation. These results suggest that PCR-based genome screening, followed by the establishment of an E. coli-driven conjugation system, is an efficient strategy to maximize potentially valuable compounds and their biosynthetic genes from NSRA strains isolated from various environments.

• Korean Journal of Microbiology
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• v.25 no.3
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• pp.198-204
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• 1987

Biparental clones and recombinant clones were obtained by spheroplast fusion of Pseudomonas putida KU218R-3 and P.putida KU428. Formation of the fusion product was the most effective when the Pseudomonas spheroplast mixture were treated with 40% plyethyleneglycol(PEG) 6000 for 10min at room temperature, The fusants which selected by indirect method were obtained at an average frequency of 10.8%. Most of the fusants were biparental clones (10.4%) and the recombinant clones were produced in low yield (0.42%). Fusants, at the frequency of 4% were obtained without PEG 6000, which shows that fusion is not strictly dependant on PEG. The stability of fusants were examined. Most of the biparental clones were segregated to parental form amd late recombinants were formed on further propagation of biparental clone but the recombinant clones were nery stable.

• Resources Recycling
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• v.15 no.6 s.74
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• pp.48-55
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• 2006

This study was carried out for establishing the physical recycling technique for commercializing process on household batteries. The procedure involves shape separator, crushing, magnetic separation, classification and eddy current separation in sequence. The separation capacity was 400-600 unit cell/hr with shape separation system. The impurities such as manganese and zinc in the magnetic product were below 0.1% respectively, the concentration of iron was above 99% in spent carbon zinc battery. Also non-magnetic products are composed of 22-30% En, 16-22% Mn, 1-3% Fe in the case oi spent zinc carbon battery. The amounts of other components such as carbon rod, plastics and separator were about 37-50%. From the eddy current separation of nonferrous products, the plate-type zinc components were separated up to 96% with 2,250-2,750 meter/min of the conveyor speed.

• Korean journal of applied entomology
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• v.28 no.1
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• pp.10-15
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• 1989

This studies were carried out to selected high pathogenic nuclear polyhedrosis viruses(NPVs) against Pseudaletia(=Leucania) separata for the introduction of microbiol control of the insect NPV in Korea. Among 21 NPVs, Sesamia inferens and 4 P. separata NPV strains were highly pathogenic against P. separata when fed orchard grass leaves smeared virus suspension on the 2nd instar larvae. Three NPV strains (LsNPV-F, LsNPV-G, LsNPV-Y) were more susceptible to the younger instar than the older instar P. separata larvae when fed artificial diet mixed with the virus to the insect.

• Korean Journal of Microbiology
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• v.38 no.2
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• pp.103-106
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• 2002

Direct PCR from intact fungal cells is not readily suitable to all fungi mainly because of difficulties in rupturing the cell walls. Microwave irradiation has been proven to be useful in fungal DNA extraction protocol. Here we describe a fast template preparation method for PCR amplification from Aspefillus nidulans conidiospores using microwave irradiation. We optimized the duration far microwave irradiation, and the amount of template DNA for PCR. Amplification from samples prepared in this manner was so efficient that we could get PCR products with size enough to identify transformants. We believe that this is a time-saving procedure for screening true transformants of A. nidulans.

• Resources Recycling
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• v.22 no.4
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• pp.46-54
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• 2013

The produced quantity of waste plastics including waste vinyls was assumed as about 5 million tons per year. The quantity of waste vinyls produced from the waste recycling center among total quantity of waste plastics was estimated as about 1 million tons per year. Most of waste vinyls produced from the waste recycling center were recycled as refuse plastic fuel(RPF) or recycled feedstock material. In this study, the medium material using waste vinyls was made by the melting process of heat medium heating and the tensile strength was analyzed for checking the usable possibility of recycled waste vinyl material by comparing with the existent product. In order to use the medium material for producing the recycled product, it can be considered that the tensile strength of medium material is more than 100 $kgf/cm^2$.

• Proceedings of the Korean Information Science Society Conference
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• 2012.06c
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• pp.19-21
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• 2012

모든 암 세포는 체세포 변이를 동반한다. 따라서 암 유전체 변이 분석에 의하여 암을 발생시키는 유전자 및 진단/치료법을 찾아낼 수 있다. 본 연구에서는 차세대 시퀀싱 데이터를 이용하여 암 특이적 단이 반복 변이(copy number variation, CNV) 유형을 밝히는 새로운 알고리즘을 제안한다. 제안하는 방식은 암 환자의 정상 세포와 암세포로부터 얻어진 정상 유전체와 암 유전체를 동시 분석하여 각각 CNV 후보 영역을 추출하며, 통계적 유의성 분석을 통하여 암 특이적 CNV 후보 영역을 선별하고, 다음 후처리 과정에서 참조 표준 서열(reference sequence)에 존재하는 오류 영역 보정 작업을 수행하여 정확한 암 특이적 CNV 영역을 추출해 낸다. 또한 다수의 대용량 유전체 데이터 동시 분석을 위하여 맵리듀스(MapReduce) 기법을 기반으로 하는 병렬 수행 알고리즘을 제안한다.