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Plant Regeneration by in vitro Tissue Culture in Korean Soybean (Glycine max L.) (기내 배양을 통한 국내 콩(Glycine max L.) 품종의 식물체 재분화)

  • Kim, Dong-Gun;Kantayos, Vipada;Kim, Dong-Kwan;Park, Heung-Gyu;Kim, Haeng-Hoon;Rha, Eui-Shik;Lee, Sheong Chun;Bae, Chang-Hyu
    • Korean Journal of Plant Resources
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    • v.29 no.1
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    • pp.143-153
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    • 2016
  • Plant regeneration via organogenesis and somatic embryogenesis was investigated in Korean soybean cultivars including Cheongja 3, Jinpumkong 2, Taekwangkong and Uram. Cotyledon, cotyledon+hypocotyl and hypocotyl segments of 7-day-old seedlings were cultured on MS medium containing various concentration (0, 1, 2 and 4 ㎎/L) of BA and TDZ. The results showed that MS medium supplemented with BA 2.0 ㎎/L yielded the highest shoot formation ratio of 83.3%. In 4 cultivars, Taekwangkong showed the highest ratio of shoot formation. When various sizes of immature cotyledons (S: 1∼ 2 ㎜, M: 3∼5 ㎜, L: 6∼8 ㎜) were tested on MS medium containing 2,4-D 40 ㎎/L for somatic embryogenesis, the optimum size for embryogenic callus induction was 3∼5 ㎜ in length of immature cotyledons. In 4 cultivars, Taekwangkong showed the highest percentage of embryogenic callus induction. The results indicate that Taekwangkong is the best soybean cultivar for plant regeneration via organogenesis and embryogenic callus induction among the 4 cultivars.

Physicochemical Characteristics of Soybeans Cultivated in Different Regions and the Accompanying Soybean Curd Properties (국내 산지별 대두와 이를 이용한 두부의 이화학적 특성 분석)

  • Seo, Yu-Jin;Kim, Min-Kyoung;Lee, Seul;Hwang, In-Kyeong
    • Korean journal of food and cookery science
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    • v.26 no.4
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    • pp.441-449
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    • 2010
  • The objective of this study was to investigate the physicochemical characteristics of soybeans cultivated in different regions and the accompanying soybean curd properties. To produce soybeans with regional competitiveness and demonstrate the distinctiveness of the soybean product, four regions(Paju, Andong, Muju, Hadong) and four varieties of soybean(Daewonkong, Daepungkong, Seonyukong, Cheogja 2) were selected for these experiments. There was a significant difference in the isoflavone content of soybeans and soybean curds(p<0.05). Soybeans from Andong had the highest content of genistein, daidzein and total isoflavone and soybeans from Hadong had the lowest content of these compound. Fatty acid composition of soybeans demonstrated a significant difference according to region(p<0.05). In particular, soybeans grown under adverse environmental conditions, such as high temperature and low latitude, contained the highest composition of oleic acid and the lowest composition of linoleic acid and linolenic acid. Although fatty acid content of soybean curd had a third of the fatty acid concentration of soybean, the same characteristic was observed in the fatty acid composition of soybean curd. In addition, both soybean and soybean curd had more than 80% unsaturated fatty acids and 50% of the unsaturated fatty acid content was linoleic acid. In conclusion, these results demonstrate the significant difference of soybean and soybean curd originating from different regions and showed the transition of nutritional constituents from soybean to soybean curd as a function of environmental factors. Therefore, we must consider these factors when manufacturing soy products.

Restoration of Earthenware & Porcelain Cultural Assets using 3D Printing (3D 프린팅을 이용한 토기·자기 문화재 복원 연구)

  • Lee, Hae Soon;Wi, Koang Chul
    • Journal of Conservation Science
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    • v.31 no.2
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    • pp.131-145
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    • 2015
  • Cultural assets restoration is being considered highly as a practical way to extend the lifespan of damaged cultural assets and re-highlight their value. However, restoration process has been mostly dependent on the manual work involving the experience and skill of a person performing restoration, thereby requiring much time and effort. In recent, it became possible to apply a method allowing restoration to be performed more accurately and easily according to the material characteristics and shape of cultural assets. Namely, it is to use a method of printing out the 3D shape data computed in computer as real object by using 3D printer for the restoration that has been performed manually. The missing part of a cultural asset is computed into computer data first and is printed out by using 3D printer to undergo a simple shaping process. The result of 3D printer application showed that repetitive output and shape and figure revision of digital data were possible, and it was possible to improve the degree of completedness of restoration through test output using various types of restoration materials using various materials. For the purpose of verifying the possibility of applying 3D printer to restore missing part of earthenware, two pieces of modern reproductions, namely, the Seven Treasure Incense Burner and Earthenware with Wagon Wheel Decoration, were artificially damaged. The restoration result showed that compared to manual work, it showed better effectiveness in curtailing work time and reproducing accurate shape. On the other hand, secondary manual work was slightly needed for detailed binding and to vividly express surface texture.

A Major Antioxidative Components and Comparison of Antioxidative Activities in Black Soybean (검정콩의 주요 항산화 원인물질 및 항산화 효과의 비교)

  • Kim, Sun-Hee;Kwon, Tai-Wan;Lee, Young-Soon;Choung, Myoung-Gun;Moon, Gap-Soon
    • Korean Journal of Food Science and Technology
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    • v.37 no.1
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    • pp.73-77
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    • 2005
  • Contents of isoflavone, phenolic acids, tocopherol, and anthocyanin in black soybean (Glycine max) were measured by HPLC. To compare antioxidative activities of main black soybean components, antioxidative effects of the same levels of commercial standard components were measured by Trolox equivalent antioxidant capacity assay (TEAC). Most effective component was gentisic acid followed by anthocyanin, p-coumaric acid, ferulic acid, genistein, syringic acid, and daidzein. TEAC assay results revealed genistein in isoflavone, gentisic acid in phenolic acids, p-tocopherol in tocopherol, and anthocyanin showed highest antioxidative and synergistic acitivities, with anthocyanin showing strongest synergy effect.

Developing Extreme Drought Scenarios for Seoul based on the Long Term Precipitation Including Paleoclimatic Data (고기후 자료를 포함한 장기연속 강수자료에 의한 서울지역의 극한가뭄 시나리오 개발)

  • Jang, Ho-Won;Cho, Hyeong-Won;Kim, Tae-Woong;Lee, Joo-Heon
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.37 no.4
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    • pp.659-668
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    • 2017
  • In this study, long-term rainfall data of more than 300 years including the paleoclimatic rainfall data from Chuk Woo Kee (1777-1907), the modern observed rainfall data (1908-2015), and the climate change scenario (2016-2099), which were provided by KMA (Korea Meteorological Agency), was used to analyze the statistical characteristics of the extreme drought in the Seoul., Annual average rainfall showed an increasing trend over a entire period, and Wavelet transform analysis of SPI (Standardized Precipitation Index) which is meteorological drought index, showed 64 to 80 months (5-6 Year) of drought periods for Chuk Woo Kee and KMA data, 96 to 128 months (8 to 10 years) of drought period for climate change data. The dry spell analysis showed that the drought occurrence frequency in the ancient period was high, but frequency was gradually decreased in the modern and future periods. In addition, through the analysis of the drought magnitude, 1901 was the extreme drought year in Seoul, and 1899-1907 was the worst consecutive 9 years long term drought in Seoul.

The Fatigue Experience of Shift Work Nurses (교대근무간호사의 피로경험)

  • Kho Hyo Jung;Kim Myung Ye;Kwon Young Sook;Kim Chung Nam;Park Kyung Min;Park Jung Sook;Park Young Suk;Park Cheong Ja;Shin Young Hee;Lee Kyung Hee;Lee Byung Sook;Lee Eun Joo
    • Journal of Korean Public Health Nursing
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    • v.18 no.1
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    • pp.103-118
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    • 2004
  • The purpose of this study was to understand the process and investigate basic theory of fatigue experience of shift work nurses. The present study adopted grounded theory methodology on fatigue of shift work nurses. The participants for this study were 15 shift work nurses who were in the age of 25 to 35, the clinical experience of 2 to 14 years and the work department of ICU. ER. ward and delivery room. The data were collected from 2000 to 2003 by using interviews and observations. The contents of the interviews were tape-recorded and were drawn through repeated method. And then were analyzed into the concept, subcategories, and categories with the open coding process and axial coding was done to identify the relationships of the concepts and categories according to the paradigm models. The core category generated, which was a central phenomena of the exhaustion process. The causal condition is change events. The central condition of exhaustion were sorted as physical discomfort, decreasing vigor, psychological instability, feeling of sleeping desire, changing face impression and being heavy body. The intervening condition were discovered as social$\cdot$ psychology$\cdot$physical resist and positive$\cdot$negative interaction strategies. The consequences of the fatigue process is the short term exhaustion relief and long term residual exhaustion. The fatigue process of this study was 'break through exhaustion' of change event-exhaustion-resist-resolve intervention-adaptation. This study offers better understanding on fatigue process of shift work nurses and may facilitate more appropriate interventive strategies to support, information and knowledges according to fatigue process.

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Influence of SNR difference on the Korean speech intelligibility in classrooms (교실에서 신호대잡음비 변이가 한국어 음성명료도에 미치는 영향)

  • Park, Chan-Jae;Jo, Sung-Min;Haan, Chan-Hoon
    • The Journal of the Acoustical Society of Korea
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    • v.38 no.6
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    • pp.651-660
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    • 2019
  • The present study aims to find out the necessary speech sound level which can satisfy with the speech intelligibility in a noisy classroom environments. For this, auralized materials were made to undertake listening tests with 27 people. Speech intelligibility tests were carried out using both Consonant-Vowel-Consonant (CVC) and Phonetically Balanced Words (PBW) methods. Signal to noise ratio was changed by 5 dB for each test. As a result, it was found that speech intelligibilities are increasing with larger Signal to Noise Ratio (SNR). It was also found that there is a lot of difference of speech intelligibilities by SNR for syllables (CVC) with the Reverberation Time (RT) of 1.5 s. However, any significant difference was not found for words (PBW) in the case with RTs of below 0.8 s. Also, it was revealed through the 2-way analysis of variance (ANOVA) test that SNR is the only attentive factor which can affect the Korean speech intelligibilities for both PBW and CVC methods. Therefore, RTs below 0.8 s could be the acoustic criteria for classroom which can minimize the effects of noise. In the case with RTs larger than 0.8 s, much larger SNR is needed to give sufficient speech intelligibility.

Types and Functions of English Hedges at a syntax-pragmatics Interface (통사화용의 접합면에서 본 영어 헤지표현의 유형과 기능)

  • Hong, Sungshim
    • The Journal of the Convergence on Culture Technology
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    • v.6 no.1
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    • pp.381-388
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    • 2020
  • This paper discusses English Hedges or Hedging Expressions on the basis of their morphosyntactic-pragramatic properties within the perspective of sociolinguistics. The term, 'Hedges' for the past decades since Lakoff(1973), has received little attention from the English grammar circles such as morphosyntax and the generative grammar theories. This paper presents a more comprehensive approach to the identification, distributions, functions, and the morphosyntactic properties of English Hedges. The earlier research on English Hedges in the 70's show that hedges are metalinguistic or mitadiscourse expressions which constitute a means for executing Politeness strategy in pragmatics. Nonetheless, research from the interface of syntactic-pragmatics has been scarce. This article suggests a more complex body of English hedges that have not been extensively discussed in the literature. Additionally, their configurational domain is to be proposed as part of the PolP with [±hedged] above CP+ (or CP beyond). The ramifications of the current study are suggested in terms of comparative linguistics, EFL/ESL studies of English for global communication, and pragmatics-sensitive machine translation studies in the forseeable future.

A Study on the Social Functions of Sijo (시조의 사회적 기능 고찰 - 조선조 사회와 시조의 관계를 중심으로 -)

  • 박규홍
    • Sijohaknonchong
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    • v.19 no.1
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    • pp.127-153
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    • 2003
  • In early Josun(朝鮮) era, the scholars, genteels, and high officials in Josun dynasty paid attention to Sijo(時調) who hoped Josun society would share Confucian values. Sijo poems written by them are based upon Confucian ideology, giving an opportunity to its members to make sure their homogeneity and helping Josun dynasty sustain its regime. Gyongichega(景幾體歌) has, however, already failed to be an appropriate genre to do these functions. Nevertheless, in the late Josun dynasty when there were agitation in class hieracy, development of currency economics, maldistribution of wealth, and pursuit of enjoyment, obscene poems turned out. Consequently these songs contributed to encroaching and eventually destroying the Josun dynasty. The question that who are in charge of creating and enjoying Sasulsijo(辭說時調) cannot be answered by approaching it in the social class point of view. The range of the maker or the reader of Sasulsijo in the late Josun dynasty was much more extensive than that in the early times. Not only aristocracy or the middle society but even some of the lower class may have made and enjoyed those songs. In the meantime, it is singer-songwriters whom Park, Hyogwan blamed for their profiteering abuse of obscenity that is supposed to have been mainly reponsible for the creation of those songs. Siga is a double-edged art in its essence--the good and the bad. The lewd songs were, in the early Josun, strictly controlled but in the late Josun dynasty, were thriving due to social changes. In this context, songs based on Confucian ideology as well as the ones focused on sexual love became decayed along with the collapse of the Josun dynasty. Even though, in the light of the history of Siga, those two types of Siga are underestimated in its artistic value, they have very special social historical meaning in doing positive and negative functions for existence and destroy of the Josun dynasty.

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Study on Context-Aware SOA based on Open Service Gateway initiative platform (OSGi플렛폼 기반의 상황인식 서비스지향아키텍쳐에 관한 연구)

  • Choi, Sung-Wook;Oh, Am-Suk;Kwon, Oh-Hyun;Kang, Si-Hyeob;Hong, Soon-Goo;Choi, Hyun-Rim
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.10 no.11
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    • pp.2083-2090
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    • 2006
  • In an proposed Context-Aware SOA(Service Oriented Architecture) based OSGi(Open Service Gateway initiative) platform, Service provider manages relative kinds of services in an integrative basis from various sensors, puts each service in a SOAP (Simple Object access Protocol) message, and register thorn to the UDDI(Universal Description Discovery and Integration) server of service registry, service requester retrivel the specified kinds of services and call them to service provider. Recently most context-aware technologies for ubiquitous home network are mainly putting emphasis on RFID/USN and location-based technology. Because of this, service-oriented architecture researches have not been made enough. Under the environment of an OSGi service platform, various context-aware services are dynamically mapping from various sensors, new services are being offered for the asking of users, and existing services are changing. Accordingly, the data sharing between services provided, management of service life cycle, and the facilitation of service distribution are needed. Taking into considering all these factors, this study has suggested an Context-Aware SOA based eclipse SOA Tools Platform using OSGi platform that can transaction throughtput of more than 546 TPS of distributional Little's Law from ATAM(Architecture Tradeoff Analysis Method) while remaining stable other condition.