• Proceedings of the KSAR Conference
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• 2003.06a
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• pp.64-64
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• 2003

본 연구는 돼지의 미성숙란을 체외에서 성숙, 수정시킨 후 생산된 체외수정란을 NCSU23 체외배양액에 sodium pyruvate, hemicalcium lactate 및 glucose를 첨가해 돼지 체외수정란의 체외발육에 미치는 영향을 검토하였다. NCSU23 체외배양액에 Glucose 0 mM, 2.78 mM, 5.56 mM 및 11.12 mM을 처리하여 체외배양한 결과 분할율은 glucose처리구가 무처리구에 비해 통계적으로 유의하게 높은 성적을 나타냈으며(P<0.05), 상실배까지 체외발육률 또한 glucose처리구가 무처리구에 비해 통계적으로 유의하게 높은 성적을 나타냈다 (P<0.05). 한편 배반포까지의 체외발육율은 5.56 mM 처리구가 다른 처리구에 비해 높은 경향을 나타냈지만 통계적 유의성은 인정되지 않았다. Glucose와 0.4mM sodium pyruvate, 2mM hemicalcium lactate 공동배양 처리구와 glucose를 첨가하지 않은 처리구의 분할율은 처리구간 커다란 차이를 나타내지 않았으며, 상실배기 이상 발육된 체외 발육율도 처리구간 커다란 차이를 나타내지 않았다. 또한, 각 처리구간 분할율은 hemicalcium lactate와 glucose공동배양 처리구가 가장 좋은 성적을 나타냈지만 통계적 유의성은 인정되지 않았으며, 상실배기 이상 발육된 체외 발육율은 hemicalcium lactate만 첨가한 처리구가 가장 좋은 성적을 나타냈지만 통계적 유의성은 인정되지 않았다. Sodium pyruvate를 체외수정 후 8시간, 48시간, 96시간, 120시간에 제거하였을 때 분할율은 sodium pyruvate를 제거하는 시기가 늦어질수록 분할율이 향상되는 경향을 나타냈지만 통계적 유의성은 인정되지 않았다. 또한, 상실배기 이상 발육된 체외 발육율은 제거하는 시기가 늦어질수록 체외 발육율이 향상되는 경향을 나타냈지만 통계적 유의성은 인정되지 않았다. 본 연구의 결과 돼지 체외수정란의 체외배양시 glucose의 첨가는 체외발육을 증진시키는 결과가 있었으며, glucose와 sodium pyruvate, hemicalcium lactate 공동배양은 체외배양에 별다른 영향을 미치지 못하는 것으로 나타났다.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.34 no.2
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• pp.160-163
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• 2001

This study was performed to investigate the effect of vitamin $B_{12}$ addition on the production and hatching rate of resting egg of freshwater rotifer, Brachionus calyciflorus. Different concentrations of vitamin $B_{12}$ was added into concentrated freshwater Chlorella before fed to rotifer and rotifer was cultured in 250 mL flask. The maximum density of rotifer was 138 inds./mL at 6 ppm and it increased with an increase of vitamin $B_{12}$ addition. Mixis rate and fertilization rate of rotifer were $63.0\%$ and $89.6\%$, respectively, at 6.0 ppm. The production of resting eggs per mL was 231 eggs/mL at 1.5 ppm, but there was no significant difference among vitamin $B_{12}$ addition levels. The highest production of resting eggs per 10,000 rotifers and Chlorella dry weight (g) were 11,289 eggs and 121,252 eggs, respectively, at the 6 ppm. The hatching rate of resting egg was not significantly different among vitamin $B_{12}$ addition levels, but significantly higher than the control (without addition of vitamin $B_{12}$ ). These results showed that vitamin $B_{12}$ addition could affect production and hatching rate of resting egg of freshwater rotifer, B. calyciflorus.

• Korean Journal of Animal Reproduction
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• v.25 no.2
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• pp.131-138
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• 2001

This experiment was designed to evaluate effects of nonspecific immunostimulator(NIS) Barodon-FX(equation omitted), anionic alkali mineral complex and far-infrared radiation solution on in vivo-produced mouse and in vitro-produced bovine embryos to blastocyst development. Proportion of mouse embryos developing into blastocyst was not greater in BSA- and Barodon-added medium than in BSA-control, but there was signifcantly different(P ＜ 0.05) in hatching and hatched blastocyst development between 0.25% Barodon-and PVP-contained medium(54.7%) than PVP-control(32.5%). BOEC and GC resulted in higher proliferation rate(24∼40% and 17∼22%, respectively) in 0.25∼0.5% Barodon-added medium than in controls, but proliferation of GC and CC greatly decreased in 1∼2% Barodon-added medium. Effect of Barodon on cell proliferation greatly varied among somatic cells. Proportion of early bovine embryos developing into morula and blastocyst was significantly greater(P ＜ 0.05) in 0.5% Barodon-added medium(50% and 63.6%) than in control(31.6% and 27.4%) under co-culture with BOEC and GC, but developmental rate was not different between other Barodon treatments and control. These data indicate that effect of Barodon on cell proliferation significantly varied between somatic cells and that addition of 0.5% Barodon in BOEC-coculture system may further improve blastocyst development in early bovine embryos.

• Journal of Animal Science and Technology
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• v.50 no.3
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• pp.355-362
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• 2008

This study was carried out to evaluate effects of either organic or inorganic sources of both chromium and copper on growth performances, nutrients digestibility and fecal Cr, Cu, and Zn excretion in growing pigs. A total of 36 growing pigs((Landrace×Yorkshire)×Duroc, weighing 61.2kg in average) were allotted to 4 treatments with 3 replicates and 3 pigs per replicate. Four treatments were designated by supplemental sources of both chromium and copper as follows: ①200ppb Cr as Cr-methionine chelate(CrMet) and 200ppm Cuas copper methionine chelate(CuMet), ②200ppb Cr as CrMet and 200ppm Cu as copper sulfate(CuSO4), ③200ppb Cr as chromium chloride(CrCl3) and 200ppm Cu as CuMet, ④200ppb Cr as CrCl3 and 200ppm Cu as CuSO4. Growth performance was highest(p<0.05) in CrMet and CuMet supplemented diet treatment. Nutrients digestibility of diets was lowest(p<0.05) in CrMet and CuSO4 supplemented diet treatment, and highest(p<0.05) in CrMet and CuMet supplemented diet treatment. Fecal copper, zinc and chromium excretion was highest(p<0.05) in CrCl3 and CuSO4 supplementation treatment and lowest(p<0.05) in CrMet and CuMet supplementation treatment. This study showed a relatively high degree of utilization of Cr and Cu as well as Zn by supplementation of CrMet and CuMet compared with those of the inorganic sources.

• Journal of the Korea Concrete Institute
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• v.17 no.5 s.89
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• pp.703-708
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• 2005

The effects of polymer-cement ratio and antifoamer content on the setting time and durability of high-fluidity polymer-modified mortars using redispersible polymer powder are examined. As the result, the setting time of the polymer-modified mortars using redispersible polymer powder tends to be delayed with increasing polymer-cement ratio, regardless of the antifoamer content. The water absorption, chloride ion penetration depth and carbonation depth of the high-fluidity polymer-modified mortars using redispersible polymer powder decrease with increasing polymer-cement ratio and antifoamer content. The resistance of freezing and thawing and chemicals improvement is attributed to the improved bond between cement hydrates and aggregates because of the incorporation of redispersible polymer powder

• Journal of the Korean Chemical Society
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• v.38 no.1
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• pp.74-79
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• 1994

The participation of superoxide in initiating tissue damage derived from xenobiotics is best illustrated by paraquat intoxication. In the present study, the roles of superoxide dismutase and catalase on paraquat-induced cell injury were investigated using primary cultured rat skin fibroblast. The degree of cell injury was assassed by the conversion of reduced MTT to a blue formazan. Paraquat produced concentration-and time-related cell injury in cultured rat skin fibroblast. Paraquat induced-cell injury was aggravated by pretreatment of aminotriazol (AT: 10 mM), an catalase inhibitor, and attenuated by addition of catalase (100∼500 unit/ml). However, the effects of diethyldithiocarbamate (DDC : 10 mM), copper- and zinc-containing superoxide dismutase (Cu, Zn-SOD) inhibitor, and Cu, Zn-SOD on paraquat-induced injury were not significant. These results suggest that hydrogen peroxide might be more responsible factor than superoxide in the pathogenesis of paraquat-induced cell injury.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.5
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• pp.582-586
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• 1999

The processing conditions for producing and edible alginate film from sea tangle were investigated by measuring water vapor permeability (WVP), tensile properties and colors of film. The alginates were extracted with $3\%$ sodium carbonate ($Na_2CO_3$) solution for 5 hrs, and the alginate films were prepared with extracted sodium alginates, D-gluconic acid lactone, $CaCO_3$ and various plasticizers, As the concentration of alginate in making the film was increased, the WVP and elongation of the film were decreased and the tensile strength was increased. The higher amount of plasticizer showed the higher WVP and elongation, and the order of various plasticizers in increasing the WVP and elongation of the film was glycerol, polyethylene glycol, and sorbitol. The addition of sorbitol in the film showed the highest value (89.57) in lightness of Hunter color system and the film added polyethylene glycol had the highest values (-1,12 and 11.4) in redness and yellowness.

• Journal of Animal Science and Technology
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• v.47 no.4
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• pp.555-564
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• 2005

This study was conducted to investigate the effect of dietary supplementation of zeolite on growth performance, carcass characteristics and fecal ammonia gas release in the finishing pigs. A total of 120 crossbred finishing pigs (50.2$\pm$1.4kg, Landrace$\time$Yorkshire$\time$Duroc) were assigned to 4 treatments and randomly divided into 12 groups. They were divided between early finishing period (50~80kg) and late finishing period (80-120kg). Finishing pigs were fed on a basal diet supplemented with 0, 1, 2 and 4% levels of zeolite. Average daily gain and feed conversion were significantly (P<0.05) improved by the dietary supplementation of 4% zeolite compared with other treatments during overall period. In the finishing periods, ammonia gas emission was reduced (P<0.05) in 4% zeolite treatment more than that of the other treatments. Carcass weight and back fat thicknesses were not affected by zeolite treatments. But, the appearance of A grade pork was increased by the dietary supplementation of 4% zeolite. Feed cost was linearly decreased by increasing the supplementation of zeolite. According to this study, 4% supplement of zeolite is suitable for the improvement of carcass quality and feed conversion, the reduction of fecal ammonia gas emission and feed cost per body weight gain.

• Food Science of Animal Resources
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• v.26 no.1
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• pp.127-135
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• 2006

This experiment was carried out to examine the fermentation properties of yogurt with bovine milk and soybean milk at the mixed ratio of 2:1 and added 0.1, 0.2, 0.4 and 1.0% red ginseng extract. The effect on promoting the fermentation by additives 0.1, 0.2, 0.4 and 1.0% red ginseng extracts were higher and pH was $3.90{\sim}3.94$ when Lactobacillus acidophilus KCTC3150 and Lactobacillus salivarius ssp. salivarius CNU27 were used. Titratable acidity showed a little inhibiting due to increasing red ginseng extract content. The average viable counts of lactic acid bacteria after 15 hour culture was the highest level of $6.26{\times}10^8cfu/mL$ when Lactobacillus acidophilus KCTC3150 was used, and the additives content of red ginseng extract was 1.0% The production of lactic acid was the highest and the concentration was 332.22 mM when Lactobacillus acidophilus KCTC3150 was used, and the additives content of red ginseng extracts was 1.0% Lactose hydrolysis was completely hydrolyzed when Lactobacillus acidophilus KCTC3150 and Lactobacillus salivarius ssp. salivarius CNU27 were used. The highest viscosity of yogurt was 780 cP when Lactobacillus acidophilus KCTC3150 and Lactobacillus salivarius subsp. salivarius CNU27 were used and red ginseng extract was added 1.0% The overall acceptability, $4.17{\pm}0.64$, was the highest when Lactobacillus salivarius subsp. salivarius CNU27 was used and the additives content of red ginseng extract was 0.2%.

• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.7
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• pp.1041-1049
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• 2015

The purpose of this study was to investigate the quality characteristics of mulberry extracts added with Omija (Schisandra chinensis Baillon) in order to determine the best conditions to enhance sour taste of mulberry extracts during manufacturing. The total acidity of Omija was 5.71%, which was 20 times that of mulberry; sour taste of mulberry extracts could be enhanced by adding Omija during the extraction period. Overall, we observed reduction of pH and soluble solid contents as well as increased total acidity and anthocyanin content with a higher Omija adding ratio. In terms of taste acceptability, which is the most important parameter of beverage development, OC-3 (added with 18.75% Omija) and OC-4 (added with 25% Omija) values were $4.20{\pm}0.75$ and $4.05{\pm}0.80$, respectively, for mulberry extracts with Omija. This result was significantly different (P<0.05) from the control group (without Omija addition), which showed an OC-3/OC-4 value of $2.30{\pm}1.24$. The results of this study indicate that Omija addition at a ratio of 18.75~25% and extraction period of 10~12 days are optimum conditions to improve sour taste when developing mulberry extracts.