• 제목/요약/키워드: 철(II)

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도열병균(Magnaporthe grisea)의 Ribosomal DNA의 ITS II 부위 핵산 염기서열을 이용한 균주간 근연관계 비교 (Comparison of Relationships in Infraspecies of Magnaporthe grisea Using DNA Sequence of Internal Transcribed Spacer II Region in Ribosomal DNA)

  • 배신철;이신우;이인구;예완해;류진창
    • 한국식물병리학회지
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    • 제12권1호
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    • pp.91-98
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    • 1996

  • 벼도열병균 14개 균주와 벼 이외 화본과 식물 도열병균 12개 균주를 대상으로 rDNA의 ITS II 부위를 증폭하여 그들의 핵산 구조 차이를 분석함으로 도열병균 균주간 분류를 시도하였다. 5.8S rDNA의 3`-말단 부위와 28S rDNA의 5`-말단 부위의 sequence 중 5`-CCCGGGAATTCGCATCGATCGATCGAATGAAGA-ACGCAGC-3`와 5`-CCCGGGATCCTCCGCTTATT-GATATGC-3`를 이용하여 PCR 증폭을 하였을 때 벼도열병균 14개 균주는 동일한 길이의 단일 밴드를 형성하였으며 벼 이외 화본과 식물 도열병균에서는 레드톱 식물로부터 분리한 도열병균만이 나머지 균주보다 38bp가 더 큰 길이를 가진 밴드를 형성하였다. PCR로 증폭된 DNA를 HaeIII와 MspI 제한효소로 절단하였을 때 벼도열병균 레이스간에는 제한효소 절단에 의한 전기영동 밴드 형태 차이를 관찰할 수 없었으나, 벼 이외 화본과 식물 도열병균 12개 균주는 3군으로 구분할 수 있었다. 벼도열병균 90=054와 강아지풀에서 분리한 도열병균 G90-5, 기장에서 분리한 G88-4, 바랭이에서 분리한 G88-5 그리고 레드톱에서 분리한 RT 균주의 ITS II 부위의 DNA 염기서열 비교 분석에 의하면 G88-4와는 다른 HaeIII와 MspI 제한효소 위치를 가지고 있었기에 제한효소 절단에 의한 전기영동 형태가 상이하였다. 또한 RT균주는 HaeIII와 MspI위치가 존재하지 않았다.

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보중익기탕증(補中益氣湯證)의 병인론적(病因論的) 분석을 위한 설문문항(說問問項) 개발(開發)( II ) (Development of Questionnaires for Pathogenesis Analysis of Bojungikgitang Symptom( II ))

  • 윤태득;박영재;이상철;박영배;오환섭
    • 대한한의진단학회지
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    • 제11권2호
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    • pp.45-58
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    • 2007

  • Background and purpose: We previously developed questionnaire of Bojungikgitang systom on the Delphi method through the pathogenesis analysis. But developed a questionnaire was not verified in the clinical. So, to ensure objectivity, quantification and validity, verification is needed for questionnaire items before applying a clinical. On this study, we looked at whether questionnaire items had been validity in the clinical. Methods: Surveys conducted about 191 patients at 12 oriental medicine hospitals. Among them, patients with Bojungikgitang systom(group I) were 95, and patients with no Bojungikgitang systom(group II) were 96. We calculated that the sum of each item in the survey and then the sum was reviewed statistically significant difference through Independent samples T test between group I and II. Results: Between group I and II, the total sum survey of the percent difference is meaningful (P<0.05). Conclusions: Reliability analysis of the Bojungikgitang systom survey research is needed in the future. Also I think that research should proceed about a lot of people.

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고려인삼 광계 II Chlorophyll a/b binding Protein 유전자(CAB)의 cloning 및 식물에의 활용연구 (Cloning of CAB cDNA encoding chlorophyll a/b binding protein of photosystem II in Korean ginseng and Use in Plant)

  • 김갑식;이기원;이종철;여운형;채순용;박은경
    • 한국연초학회지
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    • 제21권2호
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    • pp.152-159
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    • 1999

  • A CAB cDNA clone(pKGCAB) encoding the light harvesting chlorophyll a/b binding protein of the semi-shade plant, Korean ginseng(Panax ginseng C. A. Meyer) was isolated by the one-way path random sequencing of ginseng cDNA library clones and transgenic tobacco plants(Nicotiana tabacum NC82) were produced by the transformation of this ginseng CAB gene in use of Agrobacterium tumefaciens LBA4404. The CAB gene showed type 1 structure of LHCP-II, 84% similarity in nucleotide sequence and 92% in amino acid sequence to that of Nicotiana tabacum CAB40, respectively. Seed germination and initial growth of the transgenic tobacco plants transformed with the cDNA fragment were accelerated under low light intensity compared with those of normal tobacco plant, that may result from the higher light sensitivity of the transgenic plants than that of the normal.

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동물성 섬유에 대한 Lac추출물의 염색성(II) -매염방법과 매염제 종류의 효과- (Dyeabilites of Lac extract onto the silk and wool fabrics(II) -Effects of mordanting methods and various mordants-)

  • 김호정;박문영;이문철
    • 한국의류학회지
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    • 제27권9_10호
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    • pp.1134-1143
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    • 2003

  • The purpose of this study is to investigate surface color change and effects of mordants on the K/S values of silk and wool fabrics dyed with Lac extract, according to the mordanting methods and conditions such as temperature, concentration and time of the mordanting bath. The results were as follow; 1 K/S values of the silk and wool fabrics according to the temperature of the mordanting bath were shown a slight decline beyond 40$^{\circ}C$. 2. Surface color of the silk changed from red to reddish purple and that of the wool showed red tone when mordanted with Aluminum postassium sulfate, Copper acetate and Copper (II) sulfate regardless of the mordanting conditions. 3. The light and dry cleaning fastness of silk and wool fabrics dyed with Lac extract according to the mordanting methods were very good.

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Wool/Bamboo 복합소재의 염색성(II)

  • 전정훈;정동석;이문철;김경태;박상운
    • 한국염색가공학회:학술대회논문집
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    • 한국염색가공학회 2004년도 추계학술발표회 논문집
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    • pp.185-189
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    • 2004

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직업병의 집단발생(II)

  • 이원철;박정일;맹광호
    • 월간산업보건
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    • 통권82호
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    • pp.15-17
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    • 1995

  • 지난 호에 이어서 직업병의 집단발생을 조사하는 방법론을 소개하고자 한다. 이 글은 Fleming LE, Ducatman, AM, Shalat SL의 Disease Cluster in Occupational Mdicine : A Protocol for Their Investigation in the Workplace(American Journal of Industrial Medicine Vol 21 pp33-47. 1992)를 참조하였다.

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