• Transactions of the Korean Society of Mechanical Engineers A
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• v.36 no.11
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• pp.1305-1310
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• 2012

The performance of a system can be affected by the variance of noise factors, which arise owing to uncertainties of the material properties and environmental factors acting on the system. For robust design optimization of the system performance, it is necessary to minimize the effect of the variance of the noise factors that are impossible to control. However, present robust design techniques consider the variation of design factors, and not the noise factors, as being important. Furthermore, it is necessary to assume a normal distribution; however, a normal distribution is often not suitable to estimate the variations. In this study, a robust design technique is proposed to consider the variation of noise factors that are estimated as non-normal distributions in a real experiment. As an example of an engineering problem, a deep-sea manganese nodule miner tracked vehicle is used to demonstrate the feasibility of the proposed method.

• Journal of Sericultural and Entomological Science
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• v.52 no.1
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• pp.6-9
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• 2014

This study was for the investigation of the stability of purified bee venom (PBV) during the treatment in the pH range from pH2 to pH9 for 24 hours, respectively. Changes of components and physiological functionalities in PBV were by evaluated silver staining, and melittin contents were measured by liquid chromatography. The antimicrobial activity against bacteria by minimum inhibitory concentration (MIC) and effect of the cell regeneration were measured by 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl tetrazolium bromide (MTT) assay using human dermal fibroblast (HDF) cell. The main proteins such as melittin and phospholipase $A_2$ showed no characteristic changes. The antimicrobial activity and effect of cell regeneration showed no difference from pH2 to pH9. From this study, we suggest that components and physiological functionalities of PBV against treated pH were kept stability at from pH2 to pH9.

• Journal of Apiculture
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• v.35 no.1
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• pp.49-54
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• 2020

Bee (Apis mellifera L.) venom is used for the treatment of various human diseases due to its known anti-inflammatory and antibacterial properties. This study investigated the effect of purified bee venom (PBV) on adipogenesis in 3T3-L1 preadipocytes. There was no cytotoxicity while cells were treated with PBV by various concentrations. In the PBV treated cells increases in fat storage were inhibited and also confirmed by oil red o staining. To understand the underlying mechanism at the molecular level were examined on the expression of the genes involved in adipogenesis by using real-time PCR. In this cell model, the mRNA level of adipogenic genes such as peroxisome-proliferator-activated receptors gamma (PPARγ) and CAAAT/enhancer binding protein alpha(C/EBPα) were decreased by PAE treatment, comparing with those of control group. Theses results suggest that PBV inhibits adipocyte differentiation in 3T3-L1 cells and can be used as an efficient natural substance to manage anti-obesity.

• The Korean Journal of Mycology
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• v.26 no.2 s.85
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• pp.194-199
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• 1998

Pholiota sp. were collected from the forest of Kangwon province (Mt. Samak, Mt. Odae and Kangwon National University Forest Experimentation) from the early of August to the end of October 1997. Among these fungi, Poliota adiposa was selected for culture experiment because it is suitable for edible mushroom. The Optimal temperature for the mycelial growth of Pholiota adiposa was in the range of $28^{\circ}C$ while that of fruit body formation was $15{\pm}1^{\circ}C$. Brown sugar as carbon source and soybean flour as nitrogen source were good for mycelial growth in commercial liquid culture. The fruit bodies of Pholiota adiposa were artifitially produced on plastic bottle including poplar sawdust and rice bran (4:1).

• Journal of Ocean Engineering and Technology
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• v.26 no.1
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• pp.41-46
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• 2012

A deep-sea manganese nodule miner consists of 4 parts: the pickup device, crusher, disposal device, and tracked vehicle. The tracked vehicle is an essential component to keep the self-propelled miner moving across deep-sea soil. The performances of the tracked vehicle are influenced by noise factors: the shear strength of the seafloor, bottom current, seafloor slope, track speed, reaction forces of flexible hose, etc. It is necessary to adopt a robust design method that improves the performances and minimizes the variation caused by noise factors. Taguchi's method, the most widely known robust design method, searches for the robust optimum using an orthogonal array composed of the product of the inner array and outer array. In this paper, we propose a new screening technique to reduce the number of input factors and apply the MRSN (Multi-Response Signal to Noise) ratio to convert multiple performances into single one in order to overcome the difficulties and limitations of using Taguchi's method in a case with many input factors and multiple performances. A test miner was already designed and tested. It has about 1/10 the capacity of a commercial one and was successfully operated at an in-shore area. Taguchi's robust design was applied to the tracked vehicle of the test miner, and design improvements were implemented for the vehicle.

• Fire Science and Engineering
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• v.26 no.4
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• pp.1-9
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• 2012

We have examined In order to compare each other from explosion and combustion characteristics about the dusts which collects from manufacturing process of wheat flour and cosmetics manufacturing process of functional Keratin removal soap at the small and medium enterprise style. We measured explosive pressure and explosive lower limit which follows in change of concentration change at the time of talc addition uses Hartman dust explosion apparatus, also measured weight loss and endothermic quantity uses DSC and TGA. The explosion test results show that increased explosive lower limit concentration and explosive pressure decreased by the increased ratio of the talc dust. And the DSC results show that heat flux and temperature decreased by the increased ratio of the talc dust. Also increased in raising temperature causes initial smoldering temperature to move towards low temperature section and the endothermic quantity increased on a large scale. Together the TGA results show that weight loss decreased by the increased ratio of the talc dust. From this research we have assured the successive dust explosion mechanism study will play a key role as a significant safety securing guideline against the dust explosion.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.13 no.3
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• pp.222-228
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• 2008

We conducted a dynamic analysis of an underwater test collector, which operates on extremely soft soil of deep-seafloor. The underwater test collector consists of nodule pick-up device, vehicle tracks, nodule crusher, loading frame and electric-electronic system. The weight of underwater test collector is about 8600 kg. The average normal pressure, that the underwater test collector supports, is about 6.0 kPa. The dynamic analysis model of underwater test collector is developed using commercial software RecurDyn-LM and Visual Fortran 90. A terramechanics model of extremely soft soil is implemented to the software based on user-written subroutine and applied to the dynamic analysis of the underwater test collector model. The dynamic responses of test collector are studied with respect to track velocities, terrain conditions, and coefficients of added mass and drag.

• Development and Reproduction
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• v.1 no.2
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• pp.141-156
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• 1997

1992년 1월부터 12월까지 1년간에 걸쳐 전북 군산, 선연리 조하애에서 채집된 개량조개, Mactra chinensis Philippi를 대상으로 생식세포 발달과 생식소 발달양상을 조사하기 위해 토과형 전자현미경으로 미세구조 변활르 관찰하였고, 정확한 산란기를 규명하기 위해 조직학적으로 생식주기를 조사하였다. 개량조개는 장웅이체이다. 난황형성과정은 난모세포의 발달정도에 따라 다르게 나타나고 있다. 전난황형성기 난모세포질 내에서는 핵주변 구여게 골지장치와 수많은 공포들 및 미토콘드리아들이 출현하고 있는데 이들은 차후, 지방적 형성에 관여한다. 난황형성전기 난모세포에서는 지방적 및 지질과립들이 핵막 근처에서 출현하여 피질층쪽으로 분산되는 반면, 같은 발달단계의 난모세포질의 피질구역에서는 피질과립들 (단백질성 난황과립)이 처음으로 생성되어 난황막 근처의 피질층에서 핵주변 구역쪽으로 분산 분포된다. 난황형성후기 난모세포에서는 세포질 내의 골지장치, 공포, 미토콘드리아, 그리고 조면소포체들이 자율합성에 의해 난황과립 형성에 관영하고 있다. 반면, 외인성 물질들인 지질형태의 과립들, 단백질성 물질 및 다량의 글리코겐 입자들이 생식상피 낸에서 출현하고 있는데, 이들 물질이 생식상피에서 난황막 구조물인 미세융모를 통해 난황형성 후기 난모세포의 난질 내로 통과해 들어가는 현상이 관찰되었다. 이와 같은 현상은 난황성성이 일어날 때에 hterosynthesis가 일어나고 있음을 시사한다. 완숙난모세포의 난경은 약 50-60 \mu m이고, 완숙정자 두부의 길이는 대략 3 \mu m이며, 미부의 길이는 약 30 \mu m정도이다. 정자 미부편모의 axoneme은 중앙의 2개의 미세소관(microtubule)과 주변에 위치한 9개의 2중 미세소관 (microtubule)으로 구성되어 있다. 본 종의 산란기는 5월에서 9월 중순에 걸쳐 일어나는데, 주산란시기는 해수수온이 22 \circ C 이상으로 상승하는 6, 7월이다. 따라서 1년에 산란 (번식)시기가 한번 일어나고 있음을 알 수 있다. 생식 주기는 초기활성기 (1-2월), 후기활성기 (2-4월), 완숙기 (4-9월), 산란기 (5-9월) 그리고 퇴화 및 비솰성기 (6-12월)의 연속적인 5단계로 구분할 수 있었다. 재생산에 가담할 수 있는 암, 수개체들의 군성숙도(%)를 조직학적으로 조사한 결과, 각장 3.5-3.9cm 범위의 개체는 55.5%이었고, 5cm 이사인 개체들은 재생산에 100% 참여하였다. 본 종의 암, 수개체들은 만 1년부터 재생산에 가담하는 것으로 추정된다.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.3
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• pp.9-15
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• 2016

We investigated >$50-{\mu}m$ marine planktonic organisms (mainly zooplankton) using a bongo net in Masan Bay and Jangmok Bay in order to harvest 75% of natural communities based on Phase-II approval regulations by the United States Coast Guard (USCG). The concentrated volume (in 1 ton) and abundance of zooplankton were $1.8{\times}10^7ind.ton^{-1}$ and $2.3{\times}10^7ind.ton^{-1}$, and their survival rates were 82.6% and 80.1%, respectively. The community structure in Jangmok Bay was similar to that in Masan Bay, and dominant species were adult and immature groups (stage IV) of genus Acartia. Harvested populations were inoculated in a 500-ton test tank. Although the population abundances were $6.0{\times}10^4ind.ton^{-1}$ for both bay samples, the mortality rates were higher in the Masan Bay population (32%) than the Jangmok Bay population (20%). We considered the reason to be that there were 30% more immature individuals of Acartia from Masan Bay than from Jangmok Bay. The younger population may have been greatly stressed by the moving process and netting gear. After applying a Ballast Water Treatment System (BWTS) using a sample form Jangmok Bay, the mortality rates in the treatment groups were found to be 100% after 0 days and 5 days, implying that the BWTS worked well. During the winter season, the zooplankton concentration method alone did not easily satisfy the approval standards of USCG Phase II (> $10{\times}10^4ind.ton^{-1}$ in the 500 ton tank). Increasing the netting frequency and additional fishing boats may be helpful in meeting the USCG Phase II biological criteria.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.1
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• pp.35-45
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• 2002

Seasonal quantitative van Veen grab sampling was conducted to characterize the composition and structure of the benthic polychaete community inhabiting the shellfish farming ground of a coastal bay system of Jiniu Bay (Korea). A total of 132 polychaete species were identified and the polychaetes accounted for about $80\%$ of overall abundance of benthic animals. There was little significant seasonal difference in densities (abundances) of polychaetes, Maximum biomass was obseued in summer (August) and minimum value was recorded in winter (February) and spring (May). Conversely, diversity and richness were lowest in summer, indicating a seasonal variability in the polychaetous community structure, The cluster analysis indicated that such a seasonal variability resulted mainly from the appearance of a few small, r-selected opportunists in spring and the tubiculous species of the family Maldanidae in summer. On the other hand, several indicator species for the organically enriched environments such as Capitelia capitata, Notoniashs Jatericeus and hmbrineris sp. showed high densities during all the study period. Density and biomass of univariate measures of community structure were significantly lower in the arkshell-farming ground of the southern area than in the non-farming sites of the bay, A similar general tendency was also found in the spatial distributions of species diversity and richness. Principal component analysis revealed the existence of different groups of benthic assemblages between the arkshell-farming ground and non-farming sites, The lack of colonization of r-selected opportunists and/or tubiculous species in the former ground seemed to contribute to the spatial differences in the composition and structure of the polychaetous communities. Although finer granulometric composition and high sulfide concentration in sediments of the arkshell-farming ground and low salinity in the northern area were likely to account for parts of the differences, other environmental variables observed were unlikely. The spatial distribution of polychaetes in Jiniu Bay may be rather closely related to the sedimentary disturbance by selection of shells for harvesting in spring.