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http://dx.doi.org/10.7852/jses.2014.52.1.6

Stability of main components and physiological activities of bee venom treated with pH  

Cho, Miran (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Han, Sangmi (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Kim, Jungmin (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Yeo, Joohong (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Hong, InPhyo (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Woo, Soonok (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Lee, Kwanggill (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Publication Information
Journal of Sericultural and Entomological Science / v.52, no.1, 2014 , pp. 6-9 More about this Journal
Abstract
This study was for the investigation of the stability of purified bee venom (PBV) during the treatment in the pH range from pH2 to pH9 for 24 hours, respectively. Changes of components and physiological functionalities in PBV were by evaluated silver staining, and melittin contents were measured by liquid chromatography. The antimicrobial activity against bacteria by minimum inhibitory concentration (MIC) and effect of the cell regeneration were measured by 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl tetrazolium bromide (MTT) assay using human dermal fibroblast (HDF) cell. The main proteins such as melittin and phospholipase $A_2$ showed no characteristic changes. The antimicrobial activity and effect of cell regeneration showed no difference from pH2 to pH9. From this study, we suggest that components and physiological functionalities of PBV against treated pH were kept stability at from pH2 to pH9.
Keywords
Apis mellifera; Bee venom; Component; pH;
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