• Journal of Soil and Groundwater Environment
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• v.14 no.3
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• pp.1-13
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• 2009

Alkalinity and total carbon contents were measured by acid neutralizing titration (ANT), back titration (BT), gravitational weighing (GW), non-dispersive infrared-total carbon (NDIR-TC) methods for assessing precision and accuracy of alkalinity and total carbon concentration in $CO_2$-rich water. Artificial $CO_2$-rich water(ACW: pH 6.3, alkalinity 68.8 meq/L, $HCO_3^-$ 2,235 mg/L) was used for comparing the measurements. When alkalinity measured in 0 hr, percent errors of all measurement were 0~12% and coefficient of variation were less than 4%. As the result of post-hoc analysis after repeated measure analysis of variance (RM-AMOVA), the differences between the pair of methods were not significant (within confidence level of 95%), which indicates that the alkalinity measured by any method could be accurate and precise when it measured just in time of sampling. In addition, alkalinity measured by ANT and NDIR-TC were not change after 24 and 48 hours open to atmosphere, which can be explained by conservative nature of alkalinity although $CO_2$ degas from ACW. On the other hand, alkalinity measured by BT and GW increased after 24 and 48 hours open to atmosphere, which was caused by relatively high concentration of measured total carbon and increasing pH. The comparison between geochemical modeling of $CO_2$ degassing and observed data showed that pH of observed ACW was higher than calculated pH. This can be happen when degassed $CO_2$ does not come out from the solution and/or exist in solution as $CO_{2(g)}$ bubble. In that case, $CO_{2(g)}$ bubble doesn't affect the pH and alkalinity. Thus alkalinity measured by ANT and NDIR-TC could not detect the $CO_2$ bubble although measured alkalinity was similar to the calculated alkalinity. Moreover, total carbon measured by ANT and NDIR-TC could be underestimated. Consequently, it is necessary to compare the alkalinity and total carbon data from various kind of methods and interpret very carefully. This study provide technical information of measurement of dissolve $CO_2$ from $CO_2$-rich water which could be natural analogue of geologic sequestration of $CO_2$.

• Korean Journal of Soil Science and Fertilizer
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• v.28 no.2
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• pp.165-175
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• 1995

To determine the effect of soil moisture stress on growth of barley and grain quality, a pot experiment was carried out for two barley varieties(Olbori and Chogangbori) by using large plastic pot(52cm in diameter and 55cm in depth) filled with sandy loam soil under rain-controlled open green house. By means of measuring soil water potential with micro tensiometer and gypsum block installed at 10cm in soil depth, soil moisture was controlled by sub-irrigation at several irigation points such as -0.05bar, -0.2bar, -0.5bar, -1.0bar, -5.0bar and -10.0bar in soil water potential. The lower soil water potential was controlled, the shorter length of stem and internode became, and the more narrow stem diameter was. Leaf area was significantly decreased when soil water potential was controlled lower than -0.5bar, although chlorophyll content of flag and first leaves was not changed so much. Weight of grain and ear was significantly decreased when soil water potential was lower than -5.0bar and the highest grain yield was obtaind in a plot where soil water potential was controlled at -0.2bar. However, the most efficient water use of Olbori and Chogangbori was obtained at -0.5bar and -1.0bar in water potentials, respectively. Crude protain content, maximum viscosity, consistency and ${\beta}$-glucan content of barley flour increased as soil water potential significantly decreased, especially below -5.0bar, but gelatination temperature decreased as soil water potential decreased.

• Journal of Bio-Environment Control
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• v.30 no.4
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• pp.383-392
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• 2021

It has been known that oyster mushrooms cultivated in facilities with thermal insulation have been strongly affected by inner environments. Forced air-circulation fans exert much direct influence on disturbing air inside the facility so the matter is of particular interest. This study is carried out to investigate the measured levels of air uniformity in a cultivation facility for oyster mushroom in the various cases that reversibly controlled air-circulation fans which drove the flow in the upward and reverse direction by turn and unidirectional fans by which the wind blew upwards only were operated from July 1 to 10. The actual survey for the selection of ongoing operation cases presented that farmers, even though there were some discrepancies, have made use of fans in a way that it paused for 5-30min after running for 5-15min by turn. The level of air uniformity in the case of adopting reversible fans revealed a slight difference of 1.4-1.8℃ (Temp.) and 7.8-8.7% (R.H.) under the condition of not using a cooler during the investigation period. By contrast, unidirectional fans showed a noticeable difference of 3.2-3.7℃ and 14.0-15.4%, which meant that air uniformity driven by reversible fans much more increased compared to that for unidirectional fans. Among the twenty operational applications considered for reversible fans, the circumstance that the wind blew upwards for 10-15min and ceased for 5-10min and blew again in the reverse direction for 10-15min in succession gave minor improvements at the level of air uniformity, but at present there was somewhat difficult to make decision on which cases were optimally best. It seems necessary that the effects of reversible fans on air uniformity as well as qualities of oyster mushrooms have to be appraised in the cultivation period and the flow visualization needs to be done to ascertain the performance of air mixture.

• (The) Research of the performance art and culture
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• no.37
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• pp.391-435
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• 2018

This is the first study in Musicology that discusses the features of Kangwon Sangkangrye(the pre-lecture ritual in Buddhism). In this thesis, I reviewed and compared the similarity and uniqueness between Kangwon Sangkangrye in "Seokmunuibom" and the one being performed these days. The musical relation between the performance shown in the historical record and the one in contemporary ritual was illustrated here based on the findings derived from the anlaysis on its bell(小鐘) performance. The analysis made upon musical sources recorded on-site shows the characteristics of contemporary Kangwon Sangkangrye. Following is a brief summary of this study. The review and comparison between Kangwon Sangkangrae in "Seokmunuibom" and the one being performed these days showed that the second type, which was significantly different from the first type, was closer to that of the historical record. The performers at Unmunsa Temple and Chungamsa Temple seemed to be following the tradition bell(小鐘) performance style which was found in the text of the historical record. Through the analysis on Kangwon Sangkangrae, I found that it has musical characteristics as follows. The vocal range of type 1 and type 2 was mostly performed within Minor 7th and didn't exceed the perfect 8th. The melodic structure of type 1 starts with do'-la of naedeureum and ended with the melody which downscaled from do' and finalized from mi to la. Usually the mode was in menari-tori but at some lecture halls a few cases found to be upscaled from sol to la which differentiates it from the typical menari-tori of folk songs. Like the typical way of traditional rites, the singing was divided into two parts: the leading call and the following choral response. Most were sung to be one syllable on one or two tones or one syllable lasting for several tones and the musical forms were varied by musical pieces. Meantime, Sangkangrae at Haeinsa Temple was differed from that of the other temples in terms of ritual procedure and vocal style. It added Korean version of and the lead vocal skipped the first phrase of the Sasul. The melodic structure of Type 2 started with do'-la of naedeureum and was finalized as the same way of Type 1. The mode of was not the same as typical menari-tori but the Jeongrye(prostration) and Balweon(a great vow) were identical to the menari-tori of folk songs. The singing was done in two forms, solo and unison, and the lyric-attachment of Gesong was one syllable for one or two tones and that of Jeongrye and Balweon were one syllable for one or two tones + one syllable for several tones. The musical form of Jeongrye is A-B-C and A-C. Balweon didn't display a certain periodicity but still maintained sense of unity and formality through repetition of the finalizing melody.

• (The) Research of the performance art and culture
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• no.36
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• pp.75-102
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• 2018

Manjungpan Chunhyangga is established by Manjung Kim Sohee. She selectively chose and arranged the songs by Jung Jungryeol, Song Mangap, Jung Eungmin, Kim Yeonsoo, Park Dongsil, and Jung Kwangsil. Kim Sohee once stated that while a major section of Chunhyangga is based on the songs by Jung Jungryeol, Song Mangap, Jung Eungmin, and Kim Yeonsoo, the whole part of Okjungga is derived from Park Dongsil. However, there is not much material that will provide enough information with regard to what musical characteristics Park Dongsilje Chunhyangga possessed. Under the circumstances, the voice of Park Dongsilje, left by Kim Sohee, triggers curiosity on Park Dongsilje Chunhyangga. It can also be construed that there must be a good reason for choosing Park Dongsilje for Okjungga, unlike other parts. Therefore, this paper attempted to find out what the unique features of Par Dongsilje are and what the reasons behind Kim Sohee's musical choice were through comparison and analysis of Kim Sohee's musical features in Okjungga. The following are the results. Firstly, Okjungga is a created verse by its nature and it does not possess specific features to a school. Therefore, the reason for choosing Park Dongsilje could have been based on musical reason rather than verse. Secondly, the lyrics of Park Dongsilje Okjungga is closer to that of Song Mangap rather than that of Jung Jungryeolje. It can be argued that it falls between the two of them. Thirdly, the tone and melody of Park Dongsilje Okjungga falls in the middle section of Dongpyenje and Supyenje. Fourthly, the analysis of Jinyangjo rhythm out of the whole Manjungpan Chunhyangga shows that there are slight differences among Song Mangapje, Jung Jungryeolje, and Jung Eungminje. Park Dongsilje is very unique in the sense that it does not use utbutim in gyemyeonjo part in Manjungpan Chunhyangga. Park Dongsilje Okjungga is a song to which the tone, melody, and sigimsae of Supyeonje is added to lyric butimsae of Dongpyeonje. It can be seemed as simple and less sophisticated because, with its simple lyric butimsae, it shows a less complicated tone utilization rather than Jung Jungryeol. With lighter decoration of music, feelings can be conveyed more directly. That is probably why Kim Sohee commented on Park Dongsilje Chunhyangga that it is the most mournful song for Chunhyang and it is the song that moves anyone who listens to the song. Consequently, Kim Sohee accepted Park Dongsilje Okjungga and she created different feelings to Manjungpan Chunhyangga which has repeatedly displayed the color of Jung Jungryeol. Combining the lyric addition of Dongpyeon style and the melody of Supyeon style can crated a different language.

• The Korean Journal of Nuclear Medicine Technology
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• v.23 no.2
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• pp.13-19
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• 2019

Purpose Tc-99m HMPAO is widely used radiopharmaceutical for brain perfusion SPECT. Tc-99m HMPAO is chemically unstable and is liable to show deterioration of labeling efficiency due to high incidence of secondary Tc-99m HMPAO complex, free pertechnetate and reduced-hydrolyzed Tc-99m. In this study, we investigated whether sialogogues administration could reduce the impurities of Tc-99m HMPAO. Materials and Methods In thirty subjects(20 male and 10 female, age range 19~89 years, mean age $60.7{\pm}14.5years$), brain perfusion SPECT were performed at basal and citric acid stimulation states consecutively after injection of 555 MBq of Tc-99m HMPAO. In the salivary glands, the uptake coefficient was calculated using Siemens processing program. Statistical comparison between before and after the citric acid stimulation performed paired t-test. P value less than 0.05 was regarded as statistically significant. Results Salivary glands uptake was $12900{\pm}3101$ counts in basal and $10677{\pm}2742$ counts in citric acid stimulation states. Unnecessary impurities in the body is much decreased after citric acid administration(t=10.78, P<0.05). The image quality was much improved after administration of citric acid and the regional cerebral perfusion was clearly from demarcated the background. Conclusion The impurity is distributed throughout the body particularly in the salivary glands and nasal mucosa when Tc-99m HMPAO brain perfusion SPECT is performed. If this impurities is not removed, the quality of the image may deteriorate, resulting in errors in visual evaluation. The use of sialogogues could be helpful for decreasing unnecessary impurities in the body.

• Journal of Applied Biological Chemistry
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• v.62 no.3
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• pp.287-292
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• 2019

Pseudomonas tolaasii causes brown blotch disease on the oyster mushroom (Pleurotus ostreatus). Various pathogenic strains of P. tolaasii were isolated and divided into three subtypes, $P1{\alpha}$, $P1{\beta}$, and $P1{\gamma}$. For phage therapy, bacteriophages against to these subtype strains were applied to mushroom cultivation and very successful to prevent from the disease. In this study, bacteriophages were isolated against the representative strains of subtype pathogens and their polyclonal antibodies were synthesized to investigate structural relationship among capsid proteins of phages. Phage preparations over $10^{10}pfu/mL$ were injected to rabbit thigh muscle and polyclonal antibodies were obtained after three times of boost injection. Titers of the antibodies obtained were over $2{\times}10^7Ab/mL$ for the phage ${\phi}6264$, $1{\times}10^6Ab/mL$ for the phage ${\phi}HK2$, and $1{\times}10^7Ab/mL$ for the phage ${\phi}HK19$ and phage ${\phi}HK23$. High specific activities were observed between antibodies and the corresponding bacteriophages. Some cross-reactivities between the antibodies and non-corresponding bacteriophages were also measured. Antibody $Ab{\phi}6264$ inactivated all phages of $P1{\alpha}$ subtype and only phage ${\phi}HK16$ among $P1{\beta}$ subtype phages. Antibody $Ab{\phi}HK23$ of $P1{\gamma}$ subtype neutralized all phages of $P1{\beta}$ subtype as well as the phage ${\phi}HK23$, showing the widest phage-inactivation range. When the structural-similarity studies of phages were investigated by using phage antibodies, closeness obtained by phylogenetic analysis of 16S rRNA genes of pathogenic strains were quite different from that of polyclonal antibody-specific structural similarity of phage capsid proteins. In conclusion, there is weak correlation between the host strain specificity of bacteriophage and its capsid structural similarity measured by phage antibodies.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.24 no.1
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• pp.106-127
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• 2019

This study aims to understand spatio-temporal variations of macrozoobenthos community in Han River (HRE), Geum River (GRE), and Nakdong River estuaries (NRE) of Korea, sampled by National Survey of Marine Ecosystem. The survey was seasonally performed at a total of 20 stations for three years (2015-2017). Sediment samples were taken three times with van Veen grab of $0.1m^2$) areal size and sieved through a 1 mm pore size mesh on site. A total of 1,008 species were identified with 602 species in HRE, 612 in GRE, and 619 in NRE, showing similar number of species between estuaries. Mean density was $1,357ind./m^2$, showing the high in NRE ($1,357ind./m^2$), mid in GRE ($1,357ind./m^2$), and low in HRE ($1,127ind./m^2$). Mean biomass was $116.8g/m^2$, showing similar variations to density ($174.2g/m^2$ in NRE, $129.0g/m^2$ in GRE, $49.0g/m^2$ in HRE). Polychaeta dominated in number of species and density in three estuaries. Biomass-dominated taxon was Mollusca in HRE and GRE, and Echinodermata in NRE. Polychaetous species dominated all three estuaries over 4% of density, such as Dispio oculata, Heteromastus filiformis and Aonides oxycephala in HRE, Heteromastus filiformis and Scoletoma longifolia in GRE, and Pseudopolydora sp. and Aphelochaeta sp. in NRE, showing various density between estuaries. Community structure was determined by various environmental variables among estuaries such as mean grain size and sorting (HRE), salinity and mean grain size (GRE), and salinity, dissolved oxygen, loss on ignition and mud content (NRE). Our study demonstrates the application of different measures to manage ecosystems in three estuaries. HRE needs to alleviate sedimentary stressors such as sand mining, land-filling, dike construction. Management of GRE should be focused on fresh water control and sedimentary stressors. In NRE, monitoring of dominant benthos and process study on hypoxia occurrence in inner Masan Bay are necessary.

• Journal of Food Hygiene and Safety
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• v.34 no.2
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• pp.158-169
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• 2019

Ergot alkaloids are mycotoxin produced by fungi of the Claviceps genus, mainly by Claviceps purpurea in EU. Recently obtained informations indicates necessity for control the ergot in imported grains. Recent occurrence data of ergot alkaloids from EU countries indicate the necessities of management and control these toxins from the imported grains like rye, wheat, oat etc. The aim of this study is to optimize the liquid chromatography-tandem mass spectrometry method for determination of ergot alkaloids (ergometrine, ergosine, ergotamine, ergocornine, ergocryptine, ergocristine and their epimers (-inines) from grain and grain-based food. The test method was optimized by extracting the sample with acetonitrile containing 2 mM ammonium carbonate, purification with Mycosep cartridge, and instrumental analysis by LC-MS/MS using Syncronis C18 column. The standard calibration curves showed linearity with correlation coefficents; $R^2$ >0.99. Mean recoveries ranged from 72.0 to 111.3% at three different fortified levels (20, 50, and $100{\mu}g/kg$). The correlation coefficient expressed as precision was within the range of 1.9-12.9%. The limit or quantifications (LOQ) ranged from 0.012 to $0.058{\mu}g/kg$. The developed analytical method met the criteria of AOAC Int. and CAC validation parameters like accuracy and sensitivity. As a result, it was confirmed that the test method developed in this study is suitable for the simultaneous analysis of six species of ergot alkaloid from grains and grain products.