• Title/Summary/Keyword: 질소충전

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Variation of nitric oxide concentrations in response to shaking stress in the Manila clam Ruditapes philippinarum (일산화질소 (nitric oxide) 정량을 통한 바지락(Ruditapes philippinarum) 의 흔들림 스트레스 측정)

  • Park, Kyung-Il
    • The Korean Journal of Malacology
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    • v.29 no.1
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    • pp.1-6
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    • 2013
  • This study aimed to evaluate the effects of shaking stress in the hemolymph of the Manila clam Ruditapes philippinarum by quantification of nitric oxide (NO) levels. The clams were divided into 3 groups as follows: clams placed in a plain container (control), clams injected with nitro-L-arginine methyl ester (NAME, an NO inhibitor), and clams in a container filled with nylon fiber at a density of $1kg/m^3$. Subsequently, each group was placed in sea water and shaken at 100 rpm for 6 h. The concentration of NO was quantified by using DAF assay and Griess assay. Both the assays showed that while shaking significantly increased the NO concentration, the NO inhibitor reduced the NO concentration in the hemolymph of the clams tested. In addition, the nylon fiber, which was used as a filler, effectively prevented the increase in NO concentration. This result suggests that measurement of NO concentration is a useful tool for evaluation of physiological stress in marine bivalves. In addition, it should be considered that a filler is necessary when dredge fishing or the suspended clam culture method is developed.

Preparation and Characteristics of Polyurethane Hybrid Sealant Modified with Polydimethylsiloxane (Polydimethylsiloxnae 변성 Polyurethane Hybrid Sealant의 제조와 그 특성)

  • Kang, Doo-Whan;Park, Seung-Woo
    • Polymer(Korea)
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    • v.35 no.5
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    • pp.488-492
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    • 2011
  • Three isocyanatopropyldimethoxysilylpolydimethylsiloxanes(IDMSi-PDMS) were synthesised from the reaction of isocyanatopropyltrimethoxysilane with monohydroxyl group termainated PDMS having different molecular weight($M_n$=5000, 10000, and 20000). Then PDMS modified polyurethane hybrid elastomer(PSMPH) were prepared from the reaction of IDMSi-PDMS with ${\alpha}$, ${\omega}$-hydroxyl group terminated polyurethane. PSMPH sealant was prepared by compounding PSMPH elastomer with additives such as plasticizer, adhesion promoter, crosslinking agent, vicosity increasing agent, inorganic filler, and catalyst at room temperature under nitrogen atmosphere. The methoxy group in the PSMPH sealant should be crosslinked with the hydroxyl group in the building stone or moisture by typical sol-gel reaction. The adhesive strength of the sealant having PDMS of $M_n$=5000 showed 40.28 kg of maxium load and 20.14 kg of break load. The shrinkage rate of the sealant having PDMS of $M_n$=20000 was 5.7% as the best result. Also, their skin over time, slump, oil content after 8 days under oil soaked paper and alkaline resistance characteristics show good results.

Impact of Pre-planting NH4+:NO3- Ratios in Inert Media on the Growth of Chinese Cabbage Plug Seedlings (혼합상토에 기비로 혼합된 질소의 NH4+:NO3- 비율이 배추의 플러그 묘 생장에 미치는 영향)

  • Sung, Jwa Kyung;Lee, Nu Ri;Choi, Jong Myung
    • Horticultural Science & Technology
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    • v.34 no.5
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    • pp.736-745
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    • 2016
  • This research was conducted to evaluate the impact of various pre-planting $NH_4{^+}:NO_3{^-}$ ratios on the growth of plug seedlings of 'Bool-am No.3' Chinese cabbage. With fixation of the pre-planting N concentrations to $300mg{\cdot}kg^{-1}$ in a peatmoss+coir dust+perlite (3.5:3.5:3, v/v/v) medium, the $NH_4{^+}:NO_3{^-}$ ratios were varied to 0:100, 27:73, 50:50, 73:27, 100:0. Then, the each of root media containing various ratios of $NH_4{^+}:NO_3{^-}$ as well as equal concentrations of other essential nutrients was packed into 72-cell plug trays. After seeds of 'Bool-am No.3' Chinese cabbage were sown, the seedling growths were measured 2 and 4 weeks after sowing. The weekly analysis of root media and end-crop tissue analysis for mineral nutrients 4 weeks after seed sowing were also conducted. As the seedlings grew up, the pH of the root media increased, however ECs in all treatments of $NH_4{^+}:NO_3{^-}$ ratios decreased. The concentrations of K, Ca and Mg in root media were higher in the treatments of $NH_4{^+}:NO_3{^-}$ (100:0) and $NH_4{^+}:NO_3{^-}$ (73:27) than those of $NH_4{^+}:NO_3{^-}$ (0:100) and $NH_4{^+}:NO_3{^-}$ (27:73) 2 weeks after seed sowing. But the concentrations of K, Ca, Mg and Zn were get lowered in all treatments and the differences among treatments were not significant 4 weeks after sowing. The highest $NH_4{^+}$ and lowest $NO_3{^-}$ concentrations of the root media were observed in the $NH_4{^+}:NO_3{^-}$ (100:0) among all treatments. Contrary to these, the treatment of $NH_4{^+}:NO_3{^-}$ (0:100) had the lowest $NH_4{^+}$ and highest $NO_3{^-}$ concentrations. The seedling growth in terms of fresh and dry weights of aerial part were the highest in the treatment of $NH_4{^+}:NO_3{^-}$ (23:73) at 2 weeks after sowing and those of $NH_4{^+}:NO_3{^-}$ (50:50) at 4 weeks after sowing. The survival rate of seedlings in $NH_4{^+}:NO_3{^-}$ (100:0) treatment were 19% and the growth of aerial part 4 weeks after sowing was the poorest among all treatments tested. The results mentioned above indicate that the pre-planting $NH_4{^+}$ ratio in inert media should not exceed 25% in plug stage 1 through 3 (until 2 true leaf development) and 50% in plug stage 4 (after 2 true leaves to transplant).

Impact of Physico·chemical Properties of Root Substrates on Growth of 'Seolhyang' Strawberry Daughter Plants Occurred through Bag Culture of Mother Plants (포트 충전용 상토의 물리·화학성이 플라스틱백 재배를 통해 발생한 '설향' 딸기의 자묘 생육에 미치는 영향)

  • Choi, Jong-Myung;Park, Ji-Young;Yoon, Moo-Kyung
    • Horticultural Science & Technology
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    • v.28 no.6
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    • pp.964-972
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    • 2010
  • Objective of this research was to determine the influence of physico.chemical properties of root substrates on growth of daughter plants that were developed through plastic bag cultivation of mother plants in 'Seolhyang' strawberry propagation. Six different formulations of root substrates for daughter plant cultivation were peatmoss + vermiculite (5:5, A), peatmoss + perlite (7:3, B), coir dust + perlite (7:3, C), coir dust + peatmoss + perlite (3.5:3.5:3.0, D), rice-hull + coir dust + perlite (2:7:1, E), and rice hull + coir dust (3:7, F). The 10 cm plastic pots filled with formulated substrates were located near the plastic bag where mother plants were growing. Then the runners and daughter plants originated from mother plants were fixed on each root substrate filled into 10 cm plastic pot and daughter plants were grown in the plastic pots. The container capacity and air space showed big differences among substrates tested. The substrates E and F had the less container capacity and the higher air space than other substrates tested. This indicates that the two substrates would have difficulties in water managements during the raising of daughter plants. The substrates of A, B, and D which contained peatmoss in formulation had higher nitrogen concentrations than those containing coir dust or rice hull. The substrates of E and F which contained rice hull had lower nitrogen, phosphorus and potassium concentrations than those that contained coir. The crown diameters of daughter plants grown in substrate A were around 13 mm which is thicker than those grown in other substrates. The fresh weights of daughter plants grown in A substrate were the heaviest followed by C, F, D, E, and B. The dry weight of daughter plants showed similar tendency to those of fresh weight. The daughter plants which had heavy fresh and dry weights and thick crown diameter are considered good seedlings. Based on this justification, the substrates of A, C and F are acceptable for daughter plant growth of 'Seolhyang' strawberry.

Impact of Pre-planting NO3:NH4 Ratios in Root Media on the Growth of Tomato Plug Seedlings (혼합상토에 기비로 혼합된 NO3:NH4 비율이 토마토 플러그 묘 생장에 미치는 영향)

  • Sung, Jwa Kyung;Lee, Nu Ri;Choi, Jong Myung
    • Horticultural Science & Technology
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    • v.34 no.5
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    • pp.727-735
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    • 2016
  • This research was conducted to investigate the influence of various pre-planting $NO_3:NH_4$ ratios in the coir dust+peatmoss+perlite (3.5:3.5:3, v/v/v) medium on the growth of tomato plug seedlings (cv. Dotaerang Dia), changes in chemical properties of root media, and tissue nutrient contents. With the fixation of N concentration to $300mg{\cdot}L^{-1}$, the $NO_3:NH_4$ ratios were adjusted to 100:0, 73:27, 50:50, 27:73, 0:100. Then, root media containing various $NO_3:NH_4$ ratios and equal concentration of other essential nutrients were filled into 50-cell plug trays and the seeds of 'Dotaerang Die' tomato were sown. The investigation of seedling growth and tissue analysis for mineral nutrient contents based on the dry weight of above-ground tissue were conducted 6 weeks after sowing. As seedlings grew, the EC decreased quickly and pH increased gradually in the all treatment media. The plant height, fresh weight and dry weight of seedlings in the treatment of 50:50 ($NO_3:NH_4$) were 29.0 cm, 13.7 g, and 1.21 g, respectively, which were the highest among treatments tested. However, the seedlings in the treatment of 0:100 ($NO_3:NH_4$) had 26.5 cm, 11.2 g, and 0.92 g in plant height, fresh weight and dry weight, respectively. These were the poorest among the treatments tested. The tissue contents of N were 2.77 to 3.22% in all the treatments. The treatment of 27:73 ($NO_3:NH_4$) had the highest contents of Fe, Mn and Zn and that of 0:100 ($NO_3:NH_4$) had the lowest contents of Mg, Na, Cu, Mn and Zn among the treatments tested. The results indicate that $NH_4$ ratio should be lower than 50% in the coir dust+peatmoss+perlite (3.5:3.5:3, v/v/v) medium for seedling growth of tomato and the optimum ratio will be used to draw up guide lines for plug seedling production.

Preparation of Powdered Smoked-Dried Mackerel Soup and Its Taste Compounds (고등어분말수우프의 제조 및 정미성분에 관한 연구)

  • LEE Eung-Ho;OH Kwang-Soo;AHN Chang-Bum;CHUNG Bu-Gil;BAE You-Kyung;HA Jin-Hwan
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.20 no.1
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    • pp.41-51
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    • 1987
  • This study was carried out to prepare powdered smoked-dried mackerel which can be used as a soup base, and to examine storage stability and the taste compounds of Products. Raw mackerel are filleted, toiled for 10 minutes and pressed to remove lipids, and then soaked in extract solution of skipjack meat. This soaked mackerel are smoked 3 times to $10-12\%$ moisture content at $80^{\circ}C$ for 8 hours. And the smoked-dried mackerel were pulverized to 50 mesh. Finally, the powdered smoked-dried mackerel were packed in a laminated film $bag(PET/Al\;foil/CPP:\;5{\mu}m/15{\mu}m/70{\mu}m,\;15\times17cm)$ with air(product C), nitrogen(product N) and oxygen absorber(product O), and then stored at room temperature for 100 days. The moisture and crude lipid content of powdered smoked-dried mackerel was $11.3-12.3\%,\;12\%$, respectively, and water activity is 0.52-0.56. And these values showed little changes during storage. The pH, VBN and amino nitrogen content increased slowly during storage. Hydrophilic and lipophilic brown pigment formation showed a tendency of increase in product(C) and showed little change in product(N) and (O). The TBA value, peroxide value and carbonyl value of product(N) and (O) were lower than those of product (C). The major fatty acids of products were 16:0, 18:1, 22:6, 18:0 and 20:5, and polyenoic acids decreased, while saturated and monoenoic acids increased during processing and storage of products. The IMP content in products were 420.2-454.2 mg/100 g and decreased slightly with storage period. And major non-volatile organic acids in products were lactic acid, succinic acid and $\alpha-ketoglutaric$ acid. In free amino acids and related compounds, major ones are histidine, alanine, hydroxyproline, lysine, glutamic acid and anserine, which occupied $80.8\%$ of total free amino acids. The taste compounds of powdered smoked-dried mackerel were free amino acids and related compounds (1,279.4 mg/100 g), non-volatile organic acids(948.1 mg/100 g), nucleotides and their related compounds (672.8 mg/100 g), total creatinine(430.4 ntg/100 g), tetaine(86.6 mg/100 g) and small amount of TMAO. The extraction condition of powdered smoked-dried mackerel in preparing soup stock is appropriate at $100^{\circ}C$ for 1 minute. Judging from the results of taste and sensory evaluation, it is concluded that the powdered smoked-dried mackerel can be used as natural flavoring substance in preparing soups and broth.

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Effects of Rice Hull Addition and Bin Wall Characteristics on Pig Slurry Composting Properties (왕겨 이용 방법과 옹벽이 돈분 퇴비화에 미치는 효과)

  • ;Craig, Ian P
    • Journal of Animal Environmental Science
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    • v.10 no.1
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    • pp.47-58
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    • 2004
  • This work was carried out to investigate the effects of rice hull continuously utilized and/or replenished on the composting properties and to obtain the fundamental data between an unsupported wall and a soil supported wall during the period of composting with pig slurry in winter season. There were no the temperature holding effects in soil supported wall. New compost facility design for the temperature holding effects from a soil supported wall was required. The results were as follows; 1. Composting 1㎥ of pig slurry caused to save on 0.31㎥ of bulking agent in the unsupported wall in comparison with a soil supported wall in the rice hull single addition, and 0.45㎥ in the rice hull gradual addition. 2. The pile in the rice hull single addition had a high temperature in 4 days of composting indicating $71^{\circ}C$ and had a tendency in repeating periodically between $40^{\circ}C$ and $65^{\circ}C$ till 43 days of composting. And also the temperature of the pile was maintained between $48^{\circ}C$ and $28^{\circ}C$ after 50 days of composting. The pile of a rice hull gradual addition had the lower point of the temperature high increasingly according to adding up rice hull during the 35 days of composting. 3. The pH recorded in the rice hull single addition was higher(8.35∼10.02) compared to the rice hull gradual addition(8.6∼9.8). The pile of a rice hull single addition had a tendency in abruptly decreasing pH of the unsupported wall during the period of between 0.363$\textrm m^3$ and 0.537$\textrm m^3$ as a unit of pig slurry per rice hull. EC depending upon the way in adding rice hull was changed between 1.10 mS/$\textrm {cm}^3$ and 1.87 mS/$\textrm {cm}^3$. 4. The organic matter in an unsupported wall of the hull single addition was maintained the level of 55% during the period between 0.119㎥ and 0.363㎥ as a unit of pig slurry per rice hull while in the soil supported wall between 48 and 70. Water soluble C:N ratio was maintained between 1 and 2 in the rice hull single addition, while between 1 and 3 in the rice hull gradual addition. 5. Fertilizer constituents were detected higher level in the unsupported wall than in the soil supported wall in all treatments. This was dependant upon the input of pig slurry.

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Field Investigation of Environment Parameter in Aerobic Composting for Pig Slurry at a Scraper System (스크레파 축사에서 배출되는 돈분뇨슬러리 호기성 퇴비화의 환경요인 현장조사)

  • Ryoo, Jong-Won
    • Journal of Animal Environmental Science
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    • v.14 no.3
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    • pp.183-192
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    • 2008
  • This study was carried out to investigate the temperature, water balance, evaporation and physicochemical properties during the composting with pig slurry at a scraper system. The pig slurry was composted on farm trial using continuous aeration with turning machine for 5 month. A compost facility of rectangular concrete bin with dimension of 53 m (length) ${\times}$ 4.6 m (width) ${\times}$ 2 m (height) was bedded with sawdust. The environmental parameters were monitored in period of 5 months. The results were as follows ; 1. During the composting period, the temperature was varied in the range $50{\sim}70^{\circ}C$. The temperature of compost pile was highest in middle layer and lowest in under layer. Temperature difference between middle and under area of compost pile was $5{\sim}20^{\circ}C$. 2. The water content of compost pile varied $50{\sim}68%$. In the period of 50% of water content of compost pile, the temperature of compost was $20{\sim}30^{\circ}C$ and was not successfully composted. 3. In this study, total evaporation was 90% during composting. The amount of slurry per $1m^3$ sawdust by this method was $3.16m^3$ without treatment of effluent output. 4. The chemical properties of produced compost was high, but suitable for plant growth. Concentration of T-N, T-C in the final compost were 1.62, 34%, respectively.

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Effects of Hydraulic Retention Time and Temperature on Sulfur-utilizing Autotrophic Denitrification (황을 이용한 독립영양탈질에서의 체류시간과 온도의 영향)

  • Byun, Jung-Sup;Bum, Bong-Su;Cho, Kwang-Myeung
    • Journal of Korean Society of Environmental Engineers
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    • v.22 no.3
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    • pp.405-415
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    • 2000
  • Experiments for autotrophic denitrification were performed using an upf10w reac1.or packed with sulfur particles as an electron donor. The influent $NO_3{^-}$-N concentration was kept almost constant, but the hydraulic retention time(HRT) and temperature varied. Results of the research showed that the denitrification efficiency and gas generation rate decreased as the HRT and temperature were reduced. During the HRT effect experiment, alkalinities of 3.44~5.71g, with an average of 4.67 g which is close to the theoretical value of 4.57g were consumed for each gram of $NO_3{^-}$-N removed. During the temperature effect experiment, however, the values were 6.58~13.41 g with an average of 9.12 g which is almost twice the theoretical value Denitrification along the length of the reactor appeared to be a first-order reaction with a reaction rate constant of 0.1648/hr. On the other hand, the sulfate generation showed a zero-order reaction with a reaction rate constant of 241/hr. There was some discrepancy in the nitrogen mass balance between the theoretical and measured values, requiring further researches.

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Effects of Operating Conditions on Adsorption and Desorption of Benzene in TSA Process Using Activated Carbon and Zeolite 13X (활성탄과 제올라이트 13X 충진탑을 사용한 TSA 공정에서 조업조건이 벤젠의 흡착 및 탈착에 미치는 영향)

  • Jung, Min-Young;Suh, Sung-Sup
    • Applied Chemistry for Engineering
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    • v.29 no.5
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    • pp.594-603
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    • 2018
  • The effects of operating conditions such as benzene concentration, nitrogen flow rate, steam flow rate, and bed temperature on TSA process were experimentally investigated as a potential VOC removal technology using two kinds of beds packed with activated carbon and zeolite 13X. The TSA cycle studied was composed of the adsorption step, steam desorption step, and drying and cooling step. At 2% benzene concentration, the total adsorption amounts of zeolite 13X and activated carbon were 4.44 g and 3.65 g, respectively. Since the zeolite 13X has a larger packing density than that of the activated carbon, the larger benzene amount could be adsorbed in a single cycle. Increasing the water vapor flow rate to 75 g/hr at 2% benzene concentration reduced the desorption time from 1 hr to a maximum of 33 min. If the desorption time is shortened, the drying and cooling step period can be relatively increased. Accordingly, the steam removal and bed cooling could be sufficiently performed. The desorption amounts increased with the increase of the bed temperature. However, the energy consumption increased while the desorption amount was almost constant above $150^{\circ}C$. In the continuous cycle process, when the amount of remained benzene at the completion of the regeneration step increased, it might cause a decrease in the working capacity of the adsorbent. The continuous cycle process experiment for zeolite 13X showed that the amount of remained benzene at the end of regeneration step maintained a constant value after the fourth cycle.