• Environmental Analysis Health and Toxicology
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• v.24 no.4
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• pp.321-332
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• 2009

본 연구의 목적은 다이아지논(Diazinon; O, O-diethyl O-[6-methyl-2 (1-methylethyl)-4-pyrimidinyl] phosphorothioate)에 노출된 모델 생물체(송사리)의 행동변화와 관련된 분자생물학적 기전 규명을 통하여 비정상적 행동의 모니터링을 위한 생물지표(biomarker)를 개발하는데 있다. 이를 위해 우선 suppression subtractive hybridization (SSH) 및 DNA microarray 기법을 활용하여 다양한 유전자를 스크리닝하였다. 다이아지논에 노출시킨 송사리에서 발현의 차이가 나는 상향 조절된 유전자 97개 (알려지지 않은 유전자 27개 포함)와 하향 조절된 유전자 99개 (알려지지 않은 유전자 60개 포함)를 동정 하였고 이들 중 이상행동과 관련되는 것으로 보이는 유전자 10개 (상향조절 5개, 하향조절 5개)를 선발하였다. 이들 중에서 primer 제작이 잘된 beta-1, Orla C3-1, parvalbumin 및 apolipoprotein E을 선발하여 그 유전자 발현을 real-time PCR 기법을 사용하여 정량적으로 모니터링 하였다. Orla C3-1, parvalbumin 및 apolipoprotein E는 고농도의 다이아지논 처리(1000 ppb; 24 h)에서 그 발현이 억제됨이 관찰되었다. 다이아지논 처리 시 신경질환 (알츠하이머 병 및 다운신드롬)에 관련된 apolipoprotein E와 근육세포의 유연화에 작용하는 parvalbumin 등의 발현억제는 송사리의 인지능력 교란 및 근육세포의 경직 등을 각각 유도하여 송사리의 비정상적 행동을 야기하는 것으로 판단되었다. 따라서 이들 생물지표는 신경독성물질에 의한 송사리 및 기타 어류의 이상행동의 변화의 감지에 활용될 수 있을 것으로 사료된다.

• Journal of Environmental Impact Assessment
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• v.25 no.6
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• pp.466-476
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• 2016

Dredging is inevitably necessary for the management of water infrastructure such as waterways and polluted bottom sediment. Dredged material management options may be offshore dumping, wetland creation, beach nourishment and various other engineering uses depending on the given circumstances at the time of dredging. Among those options, wetland creation and beach nourishment are the preferred ones in Korea considering significant loss of wetland and beach erosion due to various development projects along the coastal region. In order to use dredged material beneficially, however, dredged material needs to be assessed its suitability with respect to its engineering purpose and environmental criteria. In this paper, we demonstrate that environmental risk of dredged material to be introduced into the marine environment can be easily assessed using biomarkers with relative low cost. Biomarkers can also compliment pollutant contents analysis that may not be specific to their impact on biological response. Biomarker information may be used to assist decision making process in selecting suitable treatment or beneficial use options for dredged materials.

• Korean Journal of Environmental Biology
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• v.36 no.1
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• pp.21-32
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• 2018

This study was carried out to define the characteristics of the identified ground-dwelling invertebrate communities at Nari basin and Tonggumi area in Ulleungdo Island, designated as a nationally important agricultural heritage. The habitat types were divided into the following categories: crop land, forest, and ecotone, and the soil-dwelling invertebrates were collected according to habitat type. The ground-dwelling invertebrates were collected using a pitfall trap, and a self-organizing map (SOM) was applied to the invertebrates dataset to define the characteristics in invertebrates distribution. The SOM clearly classified the relevant information into four clusters, and extracted ecological information from the invertebrates dataset. The cluster II was composed of invertebrate communities which are collected in the Tonggumi area. The Tonggumi area is where mountainous areas were developed for agricultural purposes, which has geographical features commonly observed in Ulleungdo Island. It is noted that the cluster II has different characteristics as compared other clusters. The results of this study are expected to be used for the preservation of agricultural environment and maintenance of biodiversity by providing basic data, on the biotope of Ulleungdo Island designated as a nationally important agricultural heritage and information on the characteristics of the applicable ground-dwelling invertebrate communities.

• River and Culture
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• v.2 no.4
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• pp.64-68
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• 2006

기존의 수환경 평각가 BOD 및 COD 개념의 이화학적 평가법 이었다면, 현재 우리나라 환경부에서 개발 추진 중에 있는 수질평가 기법은 생물 지표특성을 이용해 생태건강성을 평가한다는 점이다. 즉, 기존의 인간위주의 개념에서 탈피하여 인간과 함께 실제 생태계내에 사는 생물관점에서 평가된다는 것, 즉 수환경 관리정책의 페러다임이의 전환이라 할 수 있다.

• Korean Journal of Organic Agriculture
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• v.19 no.1
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• pp.93-107
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• 2011

This research was carried out to investigate invertebrate fauna with organic and conventional pear orchards, which used four collected methods; soil sampling for soil microorganism, pitfall, malaise, and black light trap for over ground species. Collected species were 37 species, 1,184 individuals in organic and 28 species, 501 individuals by soil sampling in conventional pear fields. Those were 38 species, 646 individuals and 29 species, 440 individuals by pitfall trap, 55 species 650 individuals and 47 species, 508 individuals by malaise trap, and 23 species, 201 individuals and 9 species, 42 individuals by black light trap. Collembola was collected 389 individuals in organic which was 5 times than in conventional in soil sampling. In pitfall trap, that was 183 individuals which was 3 times. The diversity indices of organic pear orchards were 1.956 in May, 2.638 in August and those of conventional was 1.426 in May, 2.011 in August in soil sampling. In pitfall trap, the dominant species were spiders, collembollan, and coleopteran. Among Coleoptera, indicator insects for the evaluation of agricultural environment suggested were Eusilpha jakowelewi as organic pear orchard and Anisodactykus punctatipennis and Pheropsophus jessoensis as conventional. Malaise trap was collected dominant species as Diptera and Hymenoptera of Braconidae and Ichneumonidae. The diversity indices of organic pear orchards were 2.952, 3.120, and 2.010 in pitfall, malaise and black light trap in over ground invertebrate sampling. The highest diversity was in malaise trap. The higher diversity indices, the lower dominance indices.

• Proceedings of the Zoological Society Korea Conference
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• 2001.10a
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• pp.157.3-158
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• 2001

• Bulletin of Korea Environmental Preservation Association
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• v.12 no.20
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• pp.14-18
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• 1990

• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 2004.05a
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• pp.69-69
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• 2004

• Proceedings of the Zoological Society Korea Conference
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• 1995.10b
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• pp.204.1-204
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• 1995

• Proceedings of the Korean Society of Soil Zoology Conference
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• 1996.06a
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• pp.13-13
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• 1996