• Proceedings of the Korea Water Resources Association Conference
• /
• 2009.05a
• /
• pp.1301-1305
• /
• 2009

본 연구의 목적은 금강권역에 속하는 무주 남대천유역의 유량변화가 유역의 대표어종인 피라미 서식환경에 미치는 영향을 분석하는 것이다. 유량변화를 분석하기 위해 미국의 Nature Conservency에서 개발한 수문변화지표법(Indicators of Hydrologic Alteration)을 사용하여 무주 남대천유역의 1966년${\sim}$2001년의 유량변화를 분석하였다. 유황특성이 바뀌는 1984년을 기준으로 1966년${\sim}$1983년과 1984년${\sim}$2001년의 두 기간으로 나누어 유량변화를 분석한 결과 유량은 증가하는 추세임을 확인할 수 있었다. 따라서 무주 남대천유역의 유량변화가 대표 어종인 피라미의 산란기인 4월${\sim}$5월과 치어기인 6월${\sim}$10월의 하천 서식환경에 큰 영향을 미칠 것으로 판단된다. 본 연구에서 무주 남대천유역의 산란기에 영향을 미치는 4월${\sim}$5월과 치어기에 영향을 미치는 6월${\sim}$10월의 월 평균 유량을 구분하고 수리모형인 HEC-GeoRAS를 이용하여 하도구간의 유속과 수위를 산정함으로써 피라미가 선호하는 생태적 요구조건을 만족하는 서식처 조건의 변화정도를 검토하였다.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2007.05a
• /
• pp.551-555
• /
• 2007

하구 감조부에서의 유량측정은 수문관측매뉴얼에 의하면 일반하천의 유량측정과 같은 원칙으로 실시하며, 일반 유량측정보다 신속하게 측정하고 유속과 유량을 연속적으로 측정해야 하고 조석을 잘 고려하여 관측계획을 수립하여야 하고 측정장비는 가급적 유향, 유속계의 기록부가 내장된 장비를 이용하도록 제안하고 있으며, 현실적으로 감조하천에서 유량측정은 많은 어려움이 따른다. 최근 우리나라에서 하천유량의 효율적인 측정을 위해 초음파유속계나 지표유속법과 같은 실시간 하천유량측정 방법이 도입되고 있다. 본 연구에서는 이러한 자동유량관측이 감조하천에 가능하도록 실시간 유량관측 시스템을 개발하여 운영하였다. 조석의 영향을 받는 한강대교지점에 도플러방식 초음파유량계를 회전체에 탑재한 자동유량측정장치를 남 북단 하천에 각각 1대씩 설치하였다. 본 시스템을 운영한 결과 무인 자동 연속 정밀관측이 가능하였으며, 1회 유속측정에 소요되는 $2{\sim}3$시간을 $5{\sim}6$분으로 단축시켰다. 이를 기반으로 감조하천에서의 자동유량관측이 가능하고 지표유속법을 도입하여 정확도를 높일 수 있는 유량산정기술을 개발하였다.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.28 no.6B
• /
• pp.723-729
• /
• 2008

Confidence intervals of growth curves are calculated to assess the uncertainty of index flood method as a regional frequency analysis. The asymptotic variance of quantile estimator for the generalized logistic distribution is introduced to evaluate confidence intervals. In addition, the variances of at-site frequency estimator and regional frequency estimator are used to evaluate an efficiency index. The efficiency indexes for 14 homogeneous regions based on 378 stations show that index flood method estimators are more efficient than at-site frequency estimators. It is shown that the number of sites in a region needs to be limited for regional gain.

• Korean Journal of Food Science and Technology
• /
• v.50 no.4
• /
• pp.377-382
• /
• 2018

An HPLC method was developed to quantitate a marker, oxyresveratrol (ORT), for the standardization of mulberry branch extracts (MBE) as a functional ingredient. HPLC was performed on a $C_{18}$ column with a gradient elution using 0.05% $H_3PO_4$ and acetonitrile at a flow rate of 0.8 mL/min, and detected at 320 nm. The HPLC method was validated according to Korea Food and Drug Administration (KFDA) guideline of analytical procedures with respect to specificity, linearity, accuracy and precision. Calibration curve of ORT showed high linearity ($R^2=1$), and limits of detection and quantification were 0.3 and $1.0{\mu}g/mL$, respectively. Relative standard deviation values from intra-and inter-day precision were less than 3.52 and 4.70%, respectively. Recovery rate ranged from 97.64% to 103.69%, and ORT content in MBE was approximately 3.78%. These results suggest that the HPLC method developed for the analysis of ORT in MBE is simple, efficient, and could contribute to the quality control of MBE.

• Korean Journal of Food Science and Technology
• /
• v.51 no.5
• /
• pp.420-424
• /
• 2019

An HPLC analysis method was developed and standardized for the detection of dieckol as a functional food ingredient in Ecklonia cava extracts. HPLC was performed using a Capcell Pak C18 column ($250{\times}4.6mm$, $5{\mu}m$) with a gradient elution of water and acetonitrile, both containing 0.1% (v/v) trifluoroacetic acid, at a flow rate of 1.0 mL/min at $25^{\circ}C$. The eluate was detected at 230 nm. For validation, the specificity, linearity, accuracy, precision, limit of detection (LOD), and limit of quantification (LOQ) of dieckol were measured. The calibration curve for the detection of dieckol had high linearity ($R^2=0.9994$), with LOD and LOQ values of 0.38 and $1.16{\mu}g/mL$, respectively. Recovery of the quantified compound ranged from 99.61 to 100.71%. The relative standard deviation values of the intra-day and inter-day precisions were less than 1.7 and 1.25%, respectively. These results indicate that the reported HPLC method is simple, reliable, and reproducible for the detection of dieckol in Ecklonia cava extracts.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.43 no.11
• /
• pp.1737-1741
• /
• 2014

An HPLC method for determination of naringin was developed to standardize it as a marker compound in Goheung yuzu extract as a functional health food. Optimum results were obtained by C-18 column chromatography using solvent mixtures (A: 0.5% acetic acid, B: acetonitrile) as the stationary phase and mobile phase. The method was fully validated and sensitive with a limit of detection (LOD) of 0.0218 mg/L and limit of quantification (LOQ) of 0.0661 mg/L. The method showed high linearity (coefficient of correlation=0.9986) and high accuracy, as recovery rates of naringin at concentrations of 1, 0.5, 0.1, 0.05 mg/mL were in the ranges of 95.74~98.25%, 97.67~101.01%, 97.33~104.64%, and 95.53~106.82%, respectively. Intra-day and inter-day variation, which are measures of method precision, were 1.39~1.95% and 0.17~1.49%, respectively. Therefore, the method could be used without modification for determination of naringin as a marker compound in Goheung yuzu extracts.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.40 no.4
• /
• pp.565-569
• /
• 2011

Validation of quercetin as a marker compound in the standardization of Changnyeong onion extract developed for functional health food was attempted by analytical method. The specificity was satisfied with retention time and photo diode array (PDA) spectrum by analysis of quercetin using HPLC and comparison with standard compound. It showed a high linearity in the calibration curve as coefficient of correlation ($R^2$) of 0.9986, and the limit of detection (LOD) and limit of quantitation (LOQ) were 0.2 mg/L and 0.5 mg/L, respectively. Recovery rate test with quercetin concentration of 0.05, 0.075 and 0.1 mg/mL was revealed in the high range of 82.36~95.26%, 82.70~98.24% and 87.91~95.11%, respectively. The intra-day and inter-day precision in quercetin for Changnyeong onion extracts was 0.10~3.28% and 0.96~5.79%, respectively. Therefore, application of quercetin was validated in analytical method as a marker compound in Changnyeong onion extracts.

• Journal of Food Hygiene and Safety
• /
• v.34 no.1
• /
• pp.52-57
• /
• 2019

Method development and validation of decursin for the standardization of Angelica gigas Nakai as a functional ingredient and health food were accomplished. The quantitative determination method of decursin as a marker compound of aerial parts of Angelica gigas Nakai extract (AAGE) was optimized by HPLC analysis using a C18 column ($3{\times}150mm$, $3{\mu}m$) with 0.1% TFA in water and acetonitrile as the mobile phase at a flow rate of 0.5 mL/min and detection wavelength of 330 nm. The HPLC/PDA method was applied successfully to quantification of the marker compound in AAGE after validation of the method with linearity, accuracy, and precision. The method showed high linearity in the calibration curve at a coefficient of correlation ($R^2$) of 0.9994 and the limit of detection and limit of quantitation were $0.011{\mu}g/mL$ and $0.033{\mu}g/mL$, respectively. Relative standard deviation (RSD) values of data from intra- and inter-day precision were less than 1.10% and 1.13%, respectively. Recovery of decursin at 0.5, 1, 5 and $10{\mu}g/mL$ were 92.38 ~ 104.11%. These results suggest that the developed HPLC method is very useful for the determination of marker compound in AAGE to develop a health functional material.

• Journal of Nutrition and Health
• /
• v.42 no.5
• /
• pp.486-495
• /
• 2009

The purpose of this study was to analyze the performance evaluation index for a salt reduction project. Questionnaires were developed in order to investigate salt reduction programs nationwide. The evaluation index and programs were analyzed through the case study of a salt reduction program in public health centers. The validity of the salt reduction program's evaluation index was determined based on study of the Delphi survey and on discussion with nutrition and health care professionals. The Delphi survey showed that daily salt intake was the most valid nutritional evaluation index. Stroke mortality and stomach cancer mortality were good health care evaluation indexes. The method for measuring salt intake that had the greatest validity was a 24-hour urine collection. However, 24-hour urine collection had the lowest score for ease of performance. The combined scores of validity and ease of performance showed that the survey method for dietary attitude and dietary behavior, dietary frequency analysis (DFQ 15), and a salty taste assessment, in that order, were proper methods. The high reliability of the salty taste assessment indicated that the percentage of the population that exhibits proper salt intake (2,000 mg sodium or less daily) and the percentage of the population that consumes low-salt diets as nutritional evaluation indexes also will be helpful to evaluate performance of salt reduction programs.

• Journal of Korean Society of Environmental Engineers
• /
• v.27 no.3
• /
• pp.309-315
• /
• 2005

In this study, the bacterial regrowth characteristics in drinking water were investigated for various nutrient concentrations and forms using improved BRP method as a traditional approach and specific regrowth rate as a new index. The results of bacterial regrowth potential for glucose and $NH_4^+-N$, which was evaluated by BRP method as a traditional index, appeared to be higher relative to that of acetate or humic acids as carbon source and $NO_2^--N\;or\;NO_3^--N$ as nitrogen sources, respectively. The results obtained by specific regrowth rate as a new index were similar to that of BRP method with respect to the nutrient conditions examined in this study; i.e., the specific regrowth rate for glucose(ranged from 0.005 to $0.082\;hr^{-1}$) was feater than that acetate and humic acids(ranged from 0.005 to $0.068\;hr^{-1}$ and from 0.005 to $0.008\;hr^{-1}$, respectively). And specific regrowth rate for $NH_4^+-N$ (ranged from 0.008 to $0.072\;hr^{-1}$) was feater than that $NO_2^--N\;and\;NO_3^--N$ (ranged from 0.008 to $0.055\;hr^{-1}$ and from 0.008 to $0.059\;hr^{-1}$, respectively). Therefore, specific regrowth rate can be applied in order to evaluate the bacterial regrowth potential in drinking water.