• The korean journal of orthodontics
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• v.38 no.6
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• pp.437-455
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• 2008

Condylar hyperplasia is a pathologic condition showing 3-dimensional skeletal hyperplasia of the mandible. The reason for condylar hyperplasia is not yet known, but the effects of hormone, trauma, infection, genetics, fetal condition, and hypervascularity are known as possible reasons. When we diagnose a patient as having condylar hyperplasia, it is important to decide if it is in progress or not. Treatment for facial asymmetry due to condylar hyperplasia are decided accordingly, including condylectomy, that is removal of growth site of the affected condyle, and conventional orthognathic surgery only or condylectomy with orthognathic surgery after the completion of growth. Therefore, it is important to determine the growth state of condylar hyperplasia in treatment stability. This is verified through bone scan and regular check-ups with 3D CT or PA cephalogram. This case report introduces an improved case of facial asymmetry with condylectomy together with orthognathic surgery.

• Journal of Navigation and Port Research
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• v.28 no.6
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• pp.573-578
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• 2004

The most important effects on algae multiplication are coming from maintaining the growth environment such as necessary nutrients and proper temperature, but it is difficult to adjust for every species individually. In this study, therefore, the environment is obtained using the local water where target organisms live, and their growth is promoted by raising the water temperature. A sensor to count the organism population is developed here. Because the early stage of a sudden increase of the algae population is detected using the sensor, it is available to predict the sudden increase of algae, a source of red tide.

• The Journal of Korean Orthopaedic Ultrasound Society
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• v.4 no.2
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• pp.101-110
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• 2011

To describe the background, mechanism, clinical results and complications of prolotheapy based on the literature review. Prolotherapy is a minimally invasive injection-based treatment of chronic musculoskeletal pain, including ligament and joint laxity. The mechanism of this injection-based technique is to initiate a local inflammatory response with resultant tissue healing. The used proliferants are classified by bio-mechanism to act in three different ways as osmotic, irritants, and chemotatics. The most commonly used proliferant is hyperosmolar (10~25%) dextrose to act by osmotic rupture of cells. High resolution ultrasound imaging of musculoskeletal structure provide a more accurate diagnosis. Also ultrasound-guided intervention provides a more high efficacy and low rate of complications. The most common complication is local pain at the injected site, that is self-limited and good responsive to anti-inflammatory agents. Other complications are rare. It is reported that prolotherapy appears safe when performed by an experienced clinician. Prolotherapy has grown in popularity and has received significant recent attention. However there are limited evidence-based data supporting the indication and efficacy of prolotherapy in the treatment of chronic musculoskeletal pain or soft tissue injuries. Future studies are necessary to determine whether prolotherapy can play an independent and definitive role in a treatment for chronic musculoskeletal pain.

• Journal of Life Science
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• v.19 no.11
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• pp.1514-1521
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• 2009

To evaluate the feasibility of cathepsin-B levels in preoperatively screening patients with thyroid cancer, we assigned these patients to either the thyroid cancer group (n=32) or the nodular hyperplasia group (n=7). Five healthy volunteers served as controls (n=5). We quantified cathepsin-B expressions in cancerous lesions with follicular carcinoma and hyperplastic lesions with nodular hyperplasia, and compared the degrees to those of normal thyroid tissue, which was obtained from matched contralateral lobe. The activity of serum cathepsin B was significantly higher in patients with thyroid carcinoma ($284.87{\pm}79.32$, ${\times}10^{-2}\;mU$) and those with nodular hyperplasia ($255.45{\pm}95.68$, ${\times}10^{-2}\;mU$) than compared to normal control ($168.94{\pm}15.10$, ${\times}10^{-2}\;mU$) (p<0.05). Based on the results of immunoassay, the concentrations of cathepsin B in the thyroid cancer group ($15.50{\pm}7.86\;ng/ml$) and the nodular hyperplasia group ($17.64{\pm}7.49\;ng/ml$) were higher than those of the control group ($4.85{\pm}0.61\;ng/ml$). The degree of cathepsin-B mRNA expression was significantly higher in cancerous or hyperplastic lesions than normal thyroid tissues from matched contralateral lobe with follicular carcinoma or non-neoplastic thyroid disease. Our results indicate that the activity of serum cathepsin B is a useful indicator in screening patients with nodular hyperplasia or neoplastic thyroid disease and it may be involved in the abnormal proliferation of cells.

• KSBB Journal
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• v.21 no.4
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• pp.248-254
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• 2006

Animal cell culture industry has a large market and an exponential growth rate among biological industry field. Chines hamster ovary(CHO) cells are the most widely used cell lines for recombinant protein production. They can avoid infection from polio, herpes, hepatitis B, HIV, measles, adenovirus and etc. Moreover it is easy to transfection recombinant genes and possible to suspension culture. Serum free media is one of the most important factor of protein production. Because serum has problems. Serum is not defined the contents until now, it has a number of proteins, lipids, carbohydrates and unknown molecules that cause of risk involve in infection and high cost of product purification. CHO cell line cultured using serum free media were the basis of a very successful method to produce(glyco-)protein in mammalian cells, which are then used as pharmaceutical products. Also, the low protein content of the developed medium facilitates downstream processing and product purification. But non-adapted CHO cells have a limit of proliferation cultured using serum free media and it takes very long time to adapt non-adapted cells to serum free media. There are a number of causes of a limit of proliferation using serum free media. Absence of growth factors and growth stimulating molecules is a major factor of the reasons. It makes growth signals and moves cell cycle. And increase of cellular stress is another reason. It induces increase of intraceullar ROS concentration. The purpose of this study is about improvement of proliferation capacity of non-adapted CHO cells cultured using serum free media without adaptation process.

• Parasites, Hosts and Diseases
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• v.31 no.3
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• pp.215-222
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• 1993

In order to establish a useful cell culture system for T gondii we compared the degree of proliferation of T gondii tachyzoites among 8 different cell lines: 2 kinds of normal animal cells (MDCK-canine kidney cells; Vero-monkey kidney cells) and 6 kinds of human tumor cells (A 549, PC 14-lung cancer cells; SNU 1, SNU 16. Mlm 45-stomach cancer cells; HL-60-promyelocytic leukemia cells), through morphological observation and 3H-uracil uptake assay. The degree of susceptibility to infection with T gondii tachyzoites was highest in A 549 and PC 14 cells, medium in Vero, HL-60, MDCK and SNU 1, and lowest in SNU 16 and MBm 45 cells. The kinetics of T gondii multiplication during the post-Infection 60 hours were higllly dependent upon the dose of tachyzoites administered and the duration among the 8 tested fur the growth and multiplication of T gondii in vitro.

• KSBB Journal
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• v.4 no.1
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• pp.31-33
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• 1989

The effect of growth rate change on glucose consumption and Ammonia production rate in batch culture of hybridoma was studied. The methods regulating growth rate of hybridoma were 1) decrease of serum concentration, 2) decrease of culture temperature and 3) addition of growth inhibitor (thymidine). The experimental results showed that hybridoma growth rate was dropped by 20~50%, while glucose consumption and ammonia production rate was decreased up to 40% On the other hand, the final concentration of monoclonal antibody was shown to be increased as high as 100% when the concentration of serum was decreased from 2% to 0.2%.

• Proceedings of the Korean Institute of Industrial Safety Conference
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• 2000.11a
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• pp.466-471
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• 2000

우리나라의 음식물쓰레기 처리에 관한 관심은 오래 전부터 지속되어왔고, 현재도 연구$\cdot$개발 중이다 유기성폐기물의 대표적인 음식물쓰레기는 소비하고 남은 잔재인 이상은 자연으로 돌려져야 하며 그렇지 못할 때에는 자연의 평형이 깨어지고 환경이 오염되는 것이다. 유기성폐기물은 다양한 영양물질을 함유하여 다종의 미생물을 증식할 수 있는 잠재력이 마련되어있는 자원이라 할 수 있다. 따라서 유기성물질의 분해라 하는 것은 미생물의 활발한 작용의 결과라고 볼 수 있으며, 미생물을 증식하는 과정이기도하다.(중략)

• Journal of the KSME
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• v.20 no.3
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• pp.171-175
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• 1980

증식로 system과는 대조적으로 태양 energy system은 대단히 많은 종류의 형태로 energy를 공급한다. 산림지역에서는 태양 energy는 목재와 같은 고체 연료형태로 생산되고 농업지역 에 서는 곡식에서 나오는 alcohol과 같은 야체연료, 비료나 퇴비로부터 얻어지는 methane과 같은 기체연료, 산악지방에서는 수력 발전 기상이 맑은 지방에서는 집광발전으로 거의 모든 곳에서 직접적인 열로서 얻을 수 있을 것이다. 또한 얻을 수 있는 태양 energy의 양과 종류는 지역적인 조건에 대단히 의존 될 수밖에 없다. 단 한가지의 태양 energy 생산 방식만으로는 모든 지역 에서 energy 생산 방식은 지역에 따라 각기 다르다. 그래서 전기만을 생산하는 증식로 방식에 비해 태양 energy 방식은 system의 고안에 전체적으로 복잡성을 포함하며 다양한 형태의 energy를 생산하게 될 것이다.

• Landscaping Tree
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• v.73 no.3_4
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• pp.38-41
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• 2003