• Journal of Animal Science and Technology
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• v.49 no.1
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• pp.25-32
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• 2007

The current study was undertaken to determine the effect of conjugated linoleic acid(CLA) isomers, cis-9, cis-11(c9c11), cis-9, trans-11(c9t11), trans-9, trans-11(t9t11), trans-10, cis-12(t10c12) on differentiation of pig preadipocytes and myogenic satellite cells during culture. Cells were isolated from new born pigs. The t10c12 isomer decreased differentiation of pig preadipocytes(92%), but not that of myogenic cells. The t9t11 isomer decreased differentiation of preadipocytes(14%) and increased that of myogenic cells (26%). No other CLA isomers affected differentiation of preadipocytes or myogenic cells. The effects of CLA on proliferation of preadipocytes and myogenic cells were small, compared to the effects on differentiation. These results suggest that CLA isomers have different effects on differentiaton of pig preadipocytes and myogenic cells.

• Korean Journal of Plant Tissue Culture
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• v.27 no.6
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• pp.447-451
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• 2000

This study was conducted to establish a regeneration system of plantlets through callus culture induced from bulb scales of Lillium‘Gelria’. Friable callus was induced very easily from bulb scales, and grew vigorously on medium lacking growth regulators. In media with 0.5∼ 1.0 mg/L kinetin and 0.1 ∼ 1.0 mg/L NAA, 100% of explants produced callus. Proliferation of callus was actively occurred on media containing 0.1 ∼ 1.0 mg/L kinetin and 0.1 ∼ 1.0 mg/L NAA. Callus proliferation and regeneration of bulblets from callus were occurred simultaneously. Light condition was more effective for the callus proliferation and solid medium was better than liquid medium. Althrough callus was proliferated vigorously on media containing 0.1 ∼ 1.0 mg/L BA and NAA, the frequncy of plantlet regeneration was better on medium without growth regulators, then on medium with 0.1 mg/L BA and NAA.

• The korean journal of orthodontics
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• v.36 no.6
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• pp.465-473
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• 2006

Objective: This study evaluated the effects of nitric oxide (NO) on the proliferation and differentiation of human periodontal ligament cells involved in orthodontic tooth movement. Methods: A range of concentrations of sodium nitroprusside (SNP), a NO donor, were administered to samples of human periodontal ligament cells, followed by measurement of cell viability, alkaline phosphatase (ALP) activity, and expression of osteonectin and bone sialoprotein. Results: Cell viability, ALP activity and the expression of osteonectin and bone sialoprotein were increased in human periodontal ligament cells treated with SNP concentrations of < 0.2 mM compared with controls, but were decreased with SNP concentrations of > 1.0 mM. Conclusion: NO has a biphasic effect on proliferation and differentiation in human periodontal ligament cells, with a stimulatory effect at low concentrations and an inhibitory effect at high concentrations.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.29 no.1
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• pp.102-107
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• 1984

This study was undertaken to know the possibility of in vitro propagation of Stevia through axillary bud culture and the results indicated that: (1) Addition of NAA (0.01-0.05 mg/l) alone on Murashige-Skoog basal medium promoted shoot differentiation and growth rate. And also additional of kinetin of 0.5-1.0 mg/1 alone showed the same trend as that of NAA: (2) Addition of both NAA (0.01-0.05 mg/l) and kinetin (0.5-1.0mg/l) to MS medium promoted better shoot formation. (3) Shoot differentiation and growth were better on the full salt strength of MS medium (1X MS) than that of half strength ( $\frac{1}{2}$MS), while their effects were reversed for root differentiation

• Korean Journal of Plant Tissue Culture
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• v.28 no.4
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• pp.215-219
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• 2001

This study was conducted to investigate the effect of culture media and environment on PLB proliferation by using PLBs produced from leaf segments excised from shoot of Phalanopsis flower stalk. The fresh weight of PLBs propagated was higher in MS medium than in NDM (New Dogashima medium) or VW, but the condition of PLB was better in NDM medium. Natural additives of Coconut water, potato and apple were absolutely required for the PLB propagation. PLB propagation was better in solid medium than in liquid medium including cotton as support. Optimal sucrose concentration for proliferation was 10 g/L. PLB proliferation was very effective condition 14.3 $\mu$mol.s$^{-1}$ m$^{-2}$ in PPFD and $25^{\circ}C$.

• Journal of Life Science
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• v.17 no.7 s.87
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• pp.976-982
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• 2007

The purpose of this study was to determine the modulating effects of histone deacetylase inhibitor, trichostatin A, on the differentiation of mouse C2C12 myoblasts. We demonstrated that trichostatin A induced morphological changes of C2C12 myoblasts into smooth muscles and significantly increased the gene expression of smooth muscle markers including smooth muscle ${\alpha}-actin$ and transgelin. These results were due to the change in the expression level of cell cycle regulators in trichostatin A-treated C2C12 cells. Real-time PCR data revealed that cyclin dependent kinase inhibitor, p21, mRNA expression was significantly increased in trichostatin A-treated C2C12 cells. However, trichostaDn A rapidly decreased cyclin Dl mRNA expression necessary for cell cycle progression in 24hr after treatment. In conclusion, the strong inhibitory effects of trichostatin A on histone deacetylation induced transdifferentiation of C2C12 myoblasts into smooth muscle cells and these results are partly due to the changes in the expression of cell cycle regulators such as p21 and cyclin D1.

• Korean Journal of Plant Tissue Culture
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• v.24 no.1
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• pp.43-48
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• 1997

Apical meristems of Wasabia japonica were cultured on Murashige and Skoog's medium supplemented with cytokinins alone or together with 1.0 mg/L IAA. Shoot initials could be induced from leaf primordia on apical meristems. Calli and roots were formed on the medium containing cytokinins and 1.0 mg/L IAA in combination after 30 days of culture, but there were no callus proliferation. Shoot organogenesis began after 60 days of culture and these small shoots elongated when transferred to a medium containing 1.0 mg/L BA or kinetin. Shoots were formed directly without callus induction from apical meristems all the explants on the medium containing cytokinins variously, and most of the shoots proliferated multiple shoots which could be divided to obtain plantlets. Shoot multiplication rate in response to cytokinins was best on the medium containing 1.0 mg/L BA or 2.0 mg/L zeatin. Divided plantlets rooted well on MS medium containing 0.01 mg/L IBA after 15~30 days of subculture and the rooted plantlets developed into whole plants with multiple shoots. After rooting, the regenerated plants were washed and transferred to the pots containing sterilized soil.

• Journal of Nutrition and Health
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• v.48 no.4
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• pp.327-334
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• 2015

Purpose: The objective of this study was to investigate the effects of (6)-gingerol, ginger components proliferation and adipocyte differentiation from early to lately steps. Methods: 3T3-L1 preadipocytes were cultured. Differentiation of confluent cells was induced with dexamethasone, isobutylxanthin and insulin for 2 day and cells were cultured by medium with insulin in presence of various concentrations 0, 25, 50, $100({\mu}mol/L)$ of (6)-gingerol for 4 day. Cell viability was measured using the EZ Cytox assay kit. In addition, we examined the expression of mRNA levels associated with each adipocyte differentiation step by real time reverse transcription polymerase chain reaction. Results: (6)-Gingerol inhibited adipocyte proliferation in a dose and time dependent manner. Expression of $C/EBP{\beta}$, associated with early differentiation step remained unchaged. However, intermmediate, late differentiation step and adipocytokines were effectively changed in dose-dependently manner in cell groups treated with (6)-gingerol. Conclusion: This study has shown that treatment with (6)-gingerol inhibited adipocyte proliferation as well as each adipocyte differentiation step. In particular, the (6)-gingerol more effectively inhibited adipocyte differentiation from intermmediate differentiation step.

• Journal of Periodontal and Implant Science
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• v.25 no.1
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• pp.45-55
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• 1995

치주질환의 치유에 있어서 치주인대 세포의 증식과 분화는 매우 중요하다. 몇몇 학자들에 의해 치주인대 세포의 증식과 분화에 영향을 주는 platelet derived growth나 fibronectin과 같은 growth factor에 대한 연구가 있었다. 이 연구는 홍삼 총사포닌이 치주인대 세포에 미치는 세포독성과 세포의 성장 및 분화에 미치는 영향을 규명하고자 사람의 치주인대 세포를 분리, 배양하여 실험하였다. 총사포닌이 치주인대 세포에 미치는 세포독성을 특정하기 위해 여러가지 농도의 총사포닌을 세포배양액에 첨가하여 1주일 배양후의 결과와 단일 농도($1{\mu}g/ml$})하에서의 세포 성장을 혈구계산반을 사용하여 관찰하였다. 치주인대 세포가 조골세포양세포로의 분화과정에 사포닌이 영향을 미치는 것을 관찰하기 위해 개개의 총사포닌 농도)0.1,1,$10{\mu}g/ml}$)를 세포배양액에 첨가하여 배양하였다. 치주인대 세포가 조골세포의 표현형으로 분화되는데 미치는 총사포닌의 영향을 알아보기 위하여 총사포닌의 단일 농도($1{\mu}g/ml$)하에서 $50{\mu}g/ml$ ascorbic acid와 10mM ${\beta}-glycerophosphate$를 배양액에 첨가하여 배양후 von Kossa's staining을 시행하여 생성된 골결절을 관찰하였다. 이상의 실험에서 얻어진 결과는 아래와 같다. 1. 각각의 농도를 투여한 결과, $1{\mu}g/ml$의 총사포닌에 의해서 세포독성이 유의성있게 증가하였다. 2. 0.01,0.01,1,$10{\mu}g/ml$의 총사포닌을 세포배양액에 첨가한 다음 7일 후의 고나찰시 cell viability가 실험농도 모두에서 유의성있게 증가하였다. 3. 0.1,1,10,$100{\mu}g/ml$의 농도에서 유외성있는 세포 증식이 있었다. 4. $1{\mu}g/ml$의 총사포닌을 세포배양액에 첨가한 다음 1,3,5,7,9일의 관찰시 시간경과에 따라 유의성있는 세포 증식이 있었다. 5. $10{\mu}g/ml$의 총사포닌을 세포배양액에 첨가시 ALP activity가 대조군에 비해 유외성있게 증가하였다. 6. $1{\mu}g/ml$의 총사포닌으로 배양된 치주인대 세포내에서 ALP positive cell이 관찰되었다. 7. $1{\mu}g/ml$의 총사포닌으로 배양된 치주인대 세포내에서 골결절 형성이 관찰되었다.

• Development and Reproduction
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• v.13 no.4
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• pp.297-303
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• 2009

Implantation of blastocyst into the uterine endometrium is established by the existence of histologically and functionally prepared uterine endometrium. Doc-1, an oral cancer suppressor gene, is expressed under the control of steroid hormones and has been suggested as a proliferation regulator of endometrial cells. However, the role is not much clear and in this study we examined the expression modulation of Doc-1 in decidualizing cells in vitro. In vitro decidualization was performed in endometrial stroma cells using progesterone and estrogen. Until 24 hr after decidual induction the proliferation of stroma cell was significantly increased but decreased after then. On the other hand, most of the cells differentiated into decidual cell after 48 hr of induction. The Doc-1 protein was co-localized in a specific deciudal cells and colocalization rate was increased in a parallel manner with the induction time. Based on these results, it is suggested that Doc-1 expression is under the control of both steroid hormones and decidual signals, and Doc-1 protein is involved in suppression of the proliferation of decidualizing cells.