Browse > Article

Effects of nitric oxide on the proliferation and differentiation of human periodontal ligament cells  

Choi, Sun-Young (School of Dentistry, Wonkwang University)
Cho, Jin-Hyoung (School of Dentistry, Wonkwang University)
Kang, Kyung-Hwa (School of Dentistry, Wonkwang University)
Publication Information
The korean journal of orthodontics / v.36, no.6, 2006 , pp. 465-473 More about this Journal
Abstract
Objective: This study evaluated the effects of nitric oxide (NO) on the proliferation and differentiation of human periodontal ligament cells involved in orthodontic tooth movement. Methods: A range of concentrations of sodium nitroprusside (SNP), a NO donor, were administered to samples of human periodontal ligament cells, followed by measurement of cell viability, alkaline phosphatase (ALP) activity, and expression of osteonectin and bone sialoprotein. Results: Cell viability, ALP activity and the expression of osteonectin and bone sialoprotein were increased in human periodontal ligament cells treated with SNP concentrations of < 0.2 mM compared with controls, but were decreased with SNP concentrations of > 1.0 mM. Conclusion: NO has a biphasic effect on proliferation and differentiation in human periodontal ligament cells, with a stimulatory effect at low concentrations and an inhibitory effect at high concentrations.
Keywords
Nitric oxide; Human periodontal ligament cells; Growth and differentiation;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Yokoya K, Sasaki T, Shibasaki Y. Distributional changes of osteoclasts and pre-osteoclastic cells in periodontal tissues during experimental tooth movement as revealed by quantitative immunohistochemistry of H(+)-ATP ase. J Dent Res 1997;76:580-7   DOI   ScienceOn
2 Basdra EK, Komposch G. Osteoblast-like properties of human periodontal ligament celJs: an in vitro analysis. Eur J Orthod 1997;19:615-21   DOI   ScienceOn
3 Park DK, Kim SC. The expression on nitric oxide synthetase in the experimental tooth movement in rats. Korea J Orthod 2001;31;107-20
4 Busse R, Mulsch A. Induction of nitric oxide synthase by cytokines in vascular smooth muscle cells. FEBS Lett 1990;275:87-90   DOI   ScienceOn
5 Termine JD, Belcourt AB, Conn KM, Kleinman HK. Mineral and collagen binding proteins of fetal calf bone. J Biol Chem 1981;256:10403-8
6 Ralston SH, Todd D, Helfrieh M, Benjamin N, Grabowski PS. Human osteoblast-like eells produce nitric oxide and express inducible nitric oxide synthase. Endocrinology 1994;135:330-6   DOI   ScienceOn
7 Hao J, Shi S, Niu Z, Xun Z, Yue L, Xiao M. Mineralized nodule formation by human dental papilla cells in culture. Eur J Oral Sci 1997;105:318-24   DOI   ScienceOn
8 Lowry OH, Rosebrough NJ, Farr AL, RandalJ RJ. Protein measurement with the folin-phenol reagents. J Biol Chem 1951;193:265-75
9 MacPherson H, Noble BS, Ralston SH. Expression and functional role of nitric oxide synthase isoforms in human osteoblast-like cells. Bone 1999;24:179-85   DOI   ScienceOn
10 Grieve WG 3rd, Johnson GK, Moore RN, Reinhardt RA, DuBois LM. Prostaglandin E(PGE) and interleukin-I beta (IL-1 beta) levels in gingival crevicular fluid during human orthodontic tooth movement. Am J Orthod Dentofacial Orthop 1994;105:369-74   DOI   ScienceOn
11 Minne HW, Pfeilschifter J, Scharla S, Mutschelknauss S, Schwarz A, Krempien B, Ziegler R. Inflammation-mediated osteopenia in the rat: A new animal model for pathological loss of bone mass. Endocrinology 1984;115:50-4   DOI   ScienceOn
12 Kim SW, Park DK, Kim SC, The effects of 1, 25-Dihydroxyvitamin D3 on the vitality of periodontal ligament cells and the experimental tooth movement in rats. Korea J Orthod 1997;27;335-47
13 Armour KE, Ralston SH. Estrogen upregulates endothelial constitutive nitric oxide synthase expression in human osteoblast-like cells. Endocrinology 1998; 139:799-802   DOI   ScienceOn
14 Mancini L, Moradi-Bidhendi N, Becherini L, Martineti V, Maclntyre I. The biphasic effects of nitric oxide in primary rat osteoblasts are cGMP dependent. Biochem Biophys Res Commun 2000;274:477-81   DOI   ScienceOn
15 Ralston SH, Ho LP, Helfrich MH, Grabowski PS, Johnston PW, Benjamin N. Nitric oxide: a cytokine-induced regulator of bone resorption. J Bone Miner Res 1995;10:1040-9   DOI   ScienceOn
16 Nakago-Matsuo C, Matsuo T, Nakago T. Basal nitric oxide production is enhanced by hydraulic pressure in cultured human periodontal ligament fibroblasts. Am J Orthod Dentofacial Orthop 2000; 117:474-8   DOI   ScienceOn
17 Chen RM, Liu HC, Lin YL, Jean WC, Chen JS, Wang JH. Nitric oxide induces osteoblast apoptosis through the de novo synthesis of Bax protein. J Orthop Res 2002;20:295-302   DOI   ScienceOn
18 Evans DM, Ralston SH. Nitric oxide and bone. J Bone Miner Res 1996;11:300-5   DOI   ScienceOn
19 van't Hof RJ, Ralston SH. Cytokine-indueed nitrie oxide inhibits bone resorption by indueing apoptosis of osteoclast progenitors and suppressing osteoclast aetivity. J Bone Miner Res 1997;12:1797-804   DOI   ScienceOn
20 Kim SH, Park KW, Yoo HK, Shin HS. Biological effects of pH concentration on cultured human periodontal ligament cell activity in vitro. Korea J Perio 1995;25;539-56   과학기술학회마을
21 Croueher PI, Vedi S, Motley RJ, Garrahan NJ, Stanton MR, Compston JE. Reduced bone formation in patients with osteoporosis associated with inflammatory bowel disease. Osteoporos Int 1993;3:236-41   DOI
22 Papagerakis P, Berdal A, Mesbah M, Peuchmaur M, Malaval L, Nydegger J, et al. Investigation of osteocalcin, osteoncctin, and dentin sialophosphoprotein in developing human teeth. Bone 2002;30:377-85   DOI   ScienceOn
23 Kasten TP, Collin-Osdoby P, Patel N, Osdoby P, Krukowski M, Misko TP, et al. Potentiation of osteoclast bone-resorption aetivity by inhibition of nitrie oxide synthase. Proc Natl Acad Sci USA 1994;91:3569-73
24 Bak JG, Kim SJ, Choi Jl, Effect of growth factors on the mitogenic activity of periodontal ligament cells. Korea J Perio 1994;24:572-80   과학기술학회마을
25 Mizuno M, Imai T, Fujisawa R, Tani H, Kuboki Y. Bone sialoprotein (BSP) is a crucial factor for the expression of osteoblastic phenotypes of bone marrow cells cultured on type I collagen matrix. Calcif Tissue Int 2000;66:388-96   DOI   ScienceOn
26 Kikuiri T, Hasegawa T, Yoshimura Y, Shirakawa T, Oguchi H. Cyclic tension force activates Nitric oxide production in cultured human periodontal ligament cells. J Periodontol 2000;71 :533-9   DOI   ScienceOn
27 Klein-Nulend J, Helfrich MH, Sterck JG, MacPherson H, Joldersma M, Ralston SH, et al. Nitric oxide response to shear stress by human bone cell cultures is endothelial nitric oxide synthase dependent. Biochem Biophys Res Commun 1998;250: 108-14   DOI   ScienceOn
28 Damoulis PD, Hauschka PV. Cytokines induee nitrie oxide produetion in mouse osteoblasts. Biochem Biophys Res Commun 1994;201:924-31   DOI   ScienceOn
29 Bumann A, Carvalho RS, Schwarzer CL, Yen EH. Collagen synthesis from human PDL cells folJowing orthodontic tooth movement. Eur J Orthod 1997;19:29-37   DOI   ScienceOn
30 Riancho JA, Salas E, Zarrabeitia MT, Olmos JM, Amado JA, Fernandez -Luna JL, Gonzalez-Macias J. Expression and functional role of nitric oxide synthase in osteoblast-like cells. J Bone Miner Res 1995;10:439-46   DOI   ScienceOn
31 Terry CM, Clikeman JA, Hoidal .JR, Callahan KS. TNF-a and IL-1a induce heme oxygenase-l via protein kinase C, $Ca^{2+}$, and phospholipase A2 in endothelial cells. Am J Physiol 1999;276:1493-501