• Microbiology and Biotechnology Letters
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• v.46 no.3
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• pp.253-260
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• 2018

Beneficial microorganisms are widely used in the forestry, livestock, and, in particular, agricultural sectors to control soilborne diseases and promote plant growth. However, the industrial utilization of these microorganisms is very limited, mainly due to uncertainty concerning their ability to colonize and persist in soil. In this study, the survival of beneficial microorganisms in field soil microcosms was investigated for 13 days using quantitative PCR with B. subtilis group-specific primers. Bacterial community dynamics of the treated soils were analyzed using 16S ribosomal RNA (rRNA) gene amplicon sequencing on the Illumina MiSeq platform. The average 16S rRNA gene copy number per g dry soil of Bacillus spp. was $4.37{\times}10^6$ after treatment, which was 1,000 times higher than that of the control. The gene copy number was generally maintained for a week and was reduced thereafter, but remained 100 times higher than that of the control. Bacterial community analysis indicated that Acidobacteria ($26.3{\pm}0.9%$), Proteobacteria ($24.2{\pm}0.5%$), Chloroflexi ($11.1{\pm}0.4%$), and Actinobacteria ($9.7{\pm}2.5%$) were abundant phyla in both treated and non-treated soils. In the treated soils, the relative abundance of Actinobacteria was lower, whereas those of Bacteroidetes and Firmicutes were higher compared to the control. Differences in total relative abundances of operational taxonomic units belonging to several genera were observed between the treated and non-treated soils, suggesting that inoculation of soil with the Bacillus strains influenced the relative abundances of certain groups of bacteria and, therefore, the dynamics of resident bacterial communities. These changes in resident soil bacterial communities in response to inoculation of soil with beneficial Bacillus spp. provide important information for the use of beneficial microorganisms in soil for sustainable agriculture.

• Journal of the Korean Society for Marine Environment & Energy
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• v.13 no.4
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• pp.288-295
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• 2010

Metal exposure experiments using polychaete (Perinereis nuntia) as a bio-indicator of trace metals contamination were conducted to evaluate the bioaccumulation and the biomarkers responses such as metallothionein-like protein (MTLPs) and glutathione S-transferase (GST) which was simultaneously exposed to Cadmium (Cd) and Copper (Cu). Cu and Cd concentrations in polychaete were enhanced with increasing exposure time and their concentrations of aqueous medium. Initial accumulation of Cd was higher than that of Cu. Our results showed that the bioaccumulation of Cu and Cd were prohibited, especially at higher Cu levels, suggesting the different cellular uptake mechanisms when Cu and Cd are co-exist. Net accumulation rate of Cu was declined with exposure time but it did not show any significant change for Cd. Although the highest MTLPs concentration was observed at 6 hr of exposure time, it did not show any significant change related to exposure times and metals concentrations. An increase of GST activity tended to increase as a function of exposure time and metals concentrations. And GST activities in P. nuntia have similar tendency with bioconcentration factors in high concentration of Cu (treatment group IV) at post 24 h of exposure. Our results provide new information of the bioaccumulation and biomarker responses to understand the effects of co-existing contaminants (Cu and Cd) using polychaete. Further studies are required to elucidate the bioaccumulation and biomarkers responses for various contaminants.

• Journal of the Korean Society for Marine Environment & Energy
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• v.11 no.3
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• pp.150-159
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• 2008

The marine litter generated as by-products of human activities and economic development enters to the sea through rivers or stream Indirectly, and through sea-based activities directly. It is commonly comprised of materials that degrade very slowly, such as various plastic products, polystyrene, glass, rubber, metal, wood, derelict fishing nets, wire, rope and so on. Such litter is found in the water column and on the seafloor as well as coastal areas In the Northwest Pacific region. It causes injury or death of human and other living organisms and also accident or damage of the vessel. It is not only a problem of country but also regional and/or global problem because it is transported by currents and winds from one country to another. In this regard, Northwest Pacific Action Plan (NOWPAP) Marine Litter Activity (MALITA) project had been carried out during 2006-2007 biennium and NOWPAP Regional Action Plan on Marine Litter (RAP MALI) has been also continuously implemented in the 2008-2009 biennium as next phase step of MALITA. MERRAC, one of four Regional Activity Centres (RACs) of NOWPAP, has developed monitoring guidelines, sectoral guidelines, and brochures related to sea-based marine litter and port reception facilities and services through MALITA project. Based upon these output, MERRAC will continuously implement relevant activities of RAP MALI in order to help to establish and improve a regional mechanism to deal with the sea-based marine litter problem. This paper aims to introduce MERRAC activities under MALITA and RAP MALI, and to suggest several recommendations to reduce marine litter in the NOWPAP region.

• Current Research on Agriculture and Life Sciences
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• v.3
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• pp.174-180
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• 1985

This study is designed to evaluate serum free unsaturated fatty acid, phospholipid, creatinine and prostaglandin concentrations to provide normal values for physiological barometers and preliminary information related to these chemical components on the stages of pregnancy and parturition in female rats. Forty female rats are devided into 8 groups. One control group contains 5 intact, nonpregnant female rats and the other 5 pregnancy groups contains each of 5 pregnant rats which are 9, 12, 15, 18 and 21days after pregnancy. The remaining 2 parturition groups contain each of 5 postparturient rats which are 12 and 36 hours after parturition. The results obtained are as follows ; 1. The mean concentrations of free unsaturated fatty acid in serum of the female rats ranges from 6.47 to 8.22 mg/dl. The level of pregnancy group that is 21 days being lower. 2. The mean concentrations of phospholipid in serum of the female rats ranges from 86 to 105 mg/dl. There is no marked difference between all of these groups, especially the levels of 12 days after pregnancy group and 36 hours after parturition group are lower than those of other groups. 3. The mean concentrations of crecatinine in serum of the female rats ranges from 0.64 to 0.84 mg/dl. There is no marked difference between all of 8 groups and the levels of 21 days after pregnancy group are higher than those of other groups. 4. The mean concentrations of prostaglandin in blood of the female rats rarges from 324 to $1208pg/m{\ell}$ and increases from $736pg/m{\ell}$ (18 days after pregnancy) to maximal levels of $1208pg/m{\ell}$ immediately after parturition. and then decreased progressively. Especially, each mean concentration of prostaglandin on 9, 12, or 15 days after pregnancy and mean concentrations of 5 pregnant groups are lower than those of nonpregnant female rats. 5. It is suggested that serum concentrations of free unsaturated fatty acid, phospholipid and creatinine in female rats are not related to the stages of pregnancy and parturition but prostaglandin concentrations influence the initiation of parturition in this species.

• The Journal of the Korea Contents Association
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• v.6 no.4
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• pp.146-157
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• 2006

Causal relationship means what relations the result occurred have with a fact as a reason. In general, a formular that no result exists without reasons is used for the method to confirm existence and inexistence of causal relationship. Problematic causal relationships in Private Law are reparations (Article No. 393 of Private Law) due to debt nonfulfillment and reparation due to tort (Application of Article No. 393 by Article No. 750, and No. 763 of Private Law). The purpose pursued by reparation system in private law is to promote equal burden of damages, and the range of reparation at this time is decided by the range of damage and the range of damage is decided by the principle of causal relationship. That the causal relationship theory fairly causes confusion by treating one problem and the other problem as the same thing, instead of dividing them according to the purpose of protection presented by the law is a reason of the criticism from different views.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.7
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• pp.500-509
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• 2017

This study was conducted from December 2015 to March 2016 to investigate the knowledge, attitude, and practice of baby foods in rural multi-cultural families. The survey included the general characteristics of households and infants, knowledge, attitude, and practice of baby food. The results indicated that the mean score of baby food knowledge was 3.79, 4.07 for attitudes, and the practice group was 25.0%. In the knowledge of baby food, the groups with a total number of two or more children, high educational background for both the father and mother, Chinese or Japanese nationality, high Korean language ability, and marriage for at least 6 or more years was higher (p<0.05). In the attitude of baby food, the groups with a total number of two or more children, a higher mother's Korean language, and the mother in charge of the baby food were higher (p<0.05). In the case of baby food practice, the groups the baby mother was from Japan and Cambodia, there was no occupation, and the mother was in charge of the baby food were higher (p<0.05). Knowledge and attitude according to the practice of baby food showed a higher knowledge (p <0.001) and attitude in the weaning practiced group than unpracticed. To produce baby food for infants in multi-cultural families, it is important to improve the Korean language ability of infant mothers who are in charge of baby food, and it is necessary to provide correct information related to infant formula utilizing the supporters of the neighboring mothers. In addition, it is necessary to provide customized education and support that is contingent upon the nationality of the native people while remaining mindful of the cultural implications of multi-cultural families.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.1
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• pp.537-545
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• 2018

In this study, transcriptome analysis was conducted to collect various information from Fagopyrum esculentum and Fagopyrum tataricum during the early germination stage. Total RNA was extracted from the seeds and at 12, 24, and 36 hrs after germination of Jeju native Fagopyrum esculentum and Fagopyrum tataricum and sequenced using the Illumina Hiseq 2000 platform. Raw data analysis was conducted using the Dynamic Trim and Lengths ORT programs in the SolexaQA package, and assembly and annotation were performed. Based on RNA-seq raw data, we obtained 16.5 Gb and 16.2 Gb of transcriptome data corresponding to about 84.2% and 81.5% of raw data, respectively. De novo assembly and annotation revealed 43,494 representative transcripts corresponding to 47.5Mb. Among them, 23,165 sequences were shown to have similar sequences with annotation DB. Moreover, Gene Ontology (GO) analysis of buckwheat representative transcripts confirmed that the gene is involved in metabolic processes (49.49%) of biological processes, as well as cell function (46.12%) in metabolic process, and catalytic activity (80.43%) in molecular function In the case of gibberellin receptor GID1C, which is related to germination of seeds, the expression levels increased with time after germination in both F. esculentum and F. tataricum. The expression levels of gibberellin 20-oxidase 1 were increased within 12 hrs of gemination in F. esculentum but continuously until 36 hrs in F. tataricum. This buckwheat transcriptome profile analysis of the early germination stage will help to identify the mechanism causing functional and morphological differences between species.

• Cartoon and Animation Studies
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• s.30
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• pp.113-137
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• 2013

The accessibility and effect of cartoon on children are very significant. Since cartoon is a medium of conveying information through text and pictures, it has a characteristic of being easily approached by children than any other medium. Its accessibility to children is inevitably more exceptional than any other medium in the sense that it is a method of not only telling or reading stories but also showing stories. The main audience of is children. It has a characteristic of being easily accepted by children since it's mythology seen in cartoon than read in text. However, gods conducts are full of unethical expressions. Sexual expressions of intermarriages between mother and son, between brother and sister and between uncle and niece, as well as extramarital intercourse are very unethical that could have negative effects on children. This study analyzed the unethical expressions of gods that could have negative effects on children at current point in time. Zeus was often unfaithful to his wife with many stories about the children of Zeus that were created from extramarital intercourse. Standard of value on rational decision has been established for adult readers, but children can readily accept things since they lack their own rational decision on what's right and wrong. As an alternative to children's uncritical observatory study, there is a need for author's intervention to help children form desirable values. The purpose of this study is to analyze the sexual expressions of Zeus and gods in that could affect children's social study.

• Journal of Korean Society of Environmental Engineers
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• v.33 no.4
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• pp.289-299
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• 2011

We studied the characteristics of pollutant concentrations in 9 streams that flow into Shihwa Lake in order to provide the scientific data for effective implementation of total pollution loads management system (TPLMS) of the Lake. Suspended solid (SS), chemical oxygen demand (COD), dissolved nutrients ($NO_2$, $NO_3$, $NH_4$, $PO_4$ and $SiO_2$), total phosphorus (TP) and total nitrogen (TN) in stream water from industrial complexes, urban and agricultural regions were determined. Pollutant concentrations in December were higher than that in other sampling periods. COD concentration from industrial complex region with average of 12.6 mg/L was 2 times higher those from urban region (6.6 mg/L) and agricultural region (5.9 mg/L). TP concentration from industrial region also showed higher concentration than other regions. TN concentration in stream water was 5.89 mg/L for industrial region, 3.02 mg/L for urban region and 5.27 mg/L for agricultural region, respectively, suggesting inflow of TN due to fertilizer usage in agricultural field. Relative percentage of nitrogen compounds in TN follows the sequence: $NH_4$ (35.1%) > $NO_2$ (20.0%) > DON (22.8%) > PON (8.9%) > $NO_2$ (3.2%). Concentrations of dissolved nutrients, TP and TN in stream water were 3.2~37.2 times higher than that in Shihwa Lake seawater, therefore large amount of pollutants may be directly entered into Shihwa Lake without any treatment. For Gunja stream of industrial region, pollutants at midstream showed relatively higher concentration compared to upstream and downstream. It is necessary to manage the illegal discharging of sewage and waste water. Our results provide valuable informations on the estimation and reduction of total pollutant loads in the process of establishing adequately strategic and implemental plan of Shihwa Lake TPLMS.

• Journal of Astronomy and Space Sciences
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• v.26 no.3
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• pp.305-316
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• 2009

In this study, we suggest an empirical forecast of CIR (Corotating Interaction Regions) and geomagnetic storm based on the information of coronal holes (CH). For this we used CH data obtained from He I $10830{\AA}$ maps at National Solar Observatory-Kitt Peak from January 1996 to November 2003 and the CIR and storm data that Choi et al. (2009) identified. Considering the relationship among coronal holes, CIRs, and geomagnetic storms (Choi et al. 2009), we propose the criteria for geoeffective coronal holes; the center of CH is located between $N40^{\circ}$ and $S40^{\circ}$ and between $E40^{\circ}$ and $W20^{\circ}$, and its area in percentage of solar hemispheric area is larger than the following areas: (1) case 1: 0.36%, (2) case 2: 0.66%, (3) case 3: 0.36% for 1996-2000, and 0.66% for 2001-2003. Then we present contingency tables between prediction and observation for three cases and their dependence on solar cycle phase. From the contingency tables, we determined several statistical parameters for forecast evaluation such as PODy (the probability of detection yes), FAR (the false alarm ratio), Bias (the ratio of "yes" predictions to "yes" observations) and CSI (critical success index). Considering the importance of PODy and CSI, we found that the best criterion is case 3; CH-CIR: PODy=0.77, FAR=0.66, Bias=2.28, CSI=0.30. CH-storm: PODy=0.81, FAR=0.84, Bias=5.00, CSI=0.16. It is also found that the parameters after the solar maximum are much better than those before the solar maximum. Our results show that the forecasting of CIR based on coronal hole information is meaningful but the forecast of goemagnetic storm is challenging.