• Korean Journal of Environmental Agriculture
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• v.30 no.4
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• pp.424-431
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• 2011

BACKGROUND: Tributyltin chloride is among the most toxic compounds known for aquatic ecosystems. Microorganisms are responsible for removal of TBTCl. Nevertheless, only a limited number of marine bacteria were investigated for biodegradation of TBTCl in Korea. METHODS AND RESULTS: The number of TBTCl resistant bacteria ranged from $2.5{\times}10^3$ to $3.8{\times}10^3$ cfu/mL in the seawater, and ranged from $3.2{\times}10^5$ to $9.1{\times}10^5$ cfu/g in the surface sediment, respectively. The morphological, physiological, and biochemical characteristics of TBTCl resistant bacteria were investigated by API 20NE and other tests. The most abundant species of TBTCl resistant bacteria were Vibrio spp. (19.2%), Bacillus spp. (16.2%), Aeromonas spp. (15.2%), and Pseudomonas spp. (13.1%), etc. Eleven TBTCl resistant isolates also had a resistance to heavy metals (Cd, Cu, Hg, and Zn). Among them, isolate T7 showing the strong TBTCl-resistance was selected. This isolate was identified as the genus Pantoea by 16S rRNA gene sequencing and designated as Pantoea sp. T7. In addition, this bacterium was cultivated up to the growth of 50.7% after 60 hrs at TBTCl concentration of $500{\mu}M$. TBTCl-degrading activity of Pantoea sp. T7 was measured by GC-FPD analysis. As a result of biological TBTCl-degradation at TBTCl concentration of $100{\mu}M$, TBTCl-removal efficiency of Pantoeasp. T7 was 62.7% after 40 hrs. CONCLUSION(S): These results suggest that Pantoea sp. T7 is potentially useful for the bioremediation of TBT contamination.

• Journal of Life Science
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• v.15 no.5 s.72
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• pp.826-833
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• 2005

This study was conducted to inform basic data for management of abandoned mine that was based on soil heavy metal concentration of the non-rearing and the rearing sites in Guundong Mine which had been completed of improve contamination soils, and examined into standard plant which had tolerances about pollution that analyzed into its accumulated heavy metals contents in the wild plant of naked soil. Consider form the result of contents by depth of soils heavy metals in the non-rearing sites, S1 point judged by contaminated to heavy metals of tailing leach after weathering caused by rainfall and permeate in S1 point soils when restored rest of tailing in 1996 restoration project. Lead of S2 point was not contaminated seriously by restoration and S3 point considered that heavy metals which leach by rainfall in the contaminated soils in mine vicinity accumulated in the abandoned paddy through river When heavy metals of rearing sites soils were examined from the view point of upside of mine point (SP1, SP2, and SP3) of same above the sea level, zinc (537.5 mg/kg) content of SP1 point was highest, and copper (535 mg/kg) and lead (141.5 mg/kg) in the SP3 point were high. To considered as orderly plenty of heavy metals in the plant were respectively in order, Perilla frutescens var. cauta KUDO (SP1 and SP4) >Artemisia princeps PAMPAN (SP2) > Miscanthus sinensis ANDERSSON(SP5) > Vicia angustifolia var. segetilis (THUILL.) K. KOCH. (SP2). Especially, there were plenty of heavy metals in P. frutescens var. acuta, so it judged as a standard plant which have tolerances about heavy metals pollution sites. Therefore, we need more study about the heavy metals accumulate ability of P frutescens var. acuta in future.

• Microbiology and Biotechnology Letters
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• v.32 no.4
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• pp.341-346
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• 2004

The quantification of the toxicological effects of the heavy metals such as Cu, Cd, Cr, Hg, and Zn on the growth of Escherichia coli was performed, and the variations of toxicwith exposure time were evaluated in adaptation procedure. The characteristics of growth inhibition on Escherichia coli by heavy metals were different with metal species, and critical concentration of each metal, which inhibited cell growth completely, were Cu of 3.5 mM, Zn of 2.5 mM, Cd of 1.5 mM, Cr of 1.2 mM, and Hg of 0.12 mM, respectively. The tolerance of E. coli against heavy metals, based on $EC_{50}$ values, increased in order of Cu > Zn > Cr > Cd > Hg. The slopes obtained from the relationship between ECso values and expose time corresponds to adaptability of test organisms to the toxicants. The adaptability of test organisms to the toxicants was much higher at higher slope values. Adaptability of E. coli on heavy metals increased in order to Zn > Cd > CU >> Cr> Hg.

• Journal of the Mineralogical Society of Korea
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• v.24 no.2
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• pp.83-90
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• 2011

Removal of dissolved uranium by D. baculatum, a sulfate-reducing bacterium, and effects of trace metals such as manganese, copper, nickel, and cobalt were investigated. Total concentrations of dissolved uranium and trace metals were used by $50\;{\mu}M$ and $200\;{\mu}M$, respectively. Most dissolved uranium decreased up to a non-detectable level (< 10 ppb) MS during the experiments. Most of the heavy metals did nearly not affect the bioremoval rates and amounts of uranium, but copper restrained microbial activity. However, it is found that dissolved uranium rapidly decreased after 2 weeks, showing that the bacteria can overcome the copper toxicity and remove the uranium. It is observed that nickel and cobalt were readily coprecipitated with biogenic mackinawite.

• Current Research on Agriculture and Life Sciences
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• v.11
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• pp.111-120
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• 1993

We investigated in the existential forms of heavy metals and their distribution in plant and soil surrounding ruined mine in Daduk area. The content of heavy metals in soils was more plentiful in A soil than that in another soil, excepting only Mn. The content of Mn in soils was greater in B soil than those in another soils. The content of each heavy metals, according to existential form, had different patterns on the comparision of each elements ; organic-form on Cd and As, Carbonate-form Cu, Pb and Mn and Residual-form on Fe and Zn. The popularity ration of M. senensis and J. decipiens were about 65% and 72%, respectively, and which means that these plants had resistances to heavy metals. The contents of heavy metals absorbed in root was higher than those of stem except Mn, In M. sinensis, the contents of Zn in root was similar to that of stem. To study the heavy metal contents in water, the highest content of heavy metal, mainly Fe, Mn and Zn, were calculated in extracting water.

• Journal of the Korean Institute of Landscape Architecture
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• v.42 no.5
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• pp.81-87
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• 2014

Heavy metal pollution is a widespread global problem causing serious environmental concern. Heavy metals such as Cd, Pb, and Zn can induce toxicity in all organisms if the soil levels of contaminants reach critical values. The aim of the present study was to examine the application of Liriope platyphylla, an ornamental Korean native plant with great potential for contaminated soil in urban areas, to determine tolerance for Cd, Pb, and Zn. Plants were grown in amended artificial soil with Cd, Pb, and Zn at 0, 100, 250, and $500mg{\cdot}kg^{-1}$ for 7 months. The length of leaf, width of leaf, total leaf number, dead leaf number, new leaf number, chlorophyll contents, and ornamental value were monitored from May to August, during growth the period. The relative leaf length and leaf width displayed rapidly decreasing tendencies with an increasing Cd concentration beginning from 4 months after planting. The same decreasing tendency was observed in total leaf number, new leaf number, chlorophyll contents, and ornamental values showed a trend of Control> $Cd_{100}$ > $Cd_{250}$ > $Cd_{500}$. In Pb concentration treatments, the relative leaf length and leaf width were significantly lower in plants grown at $250mg{\cdot}kg^{-1}$ and $500mg{\cdot}kg^{-1}$ as compared to the Control, $100mg{\cdot}kg^{-1}$. The total leaf number, new leaf number, and dead leaf number did not show significant difference among treatments in Control and $Pb_{100}$ but chlorophyll contents and ornamental value decreased with increasing Pb supply concentration treatments. However, in Zn supply treatments, the relative leaf length was higher at $100mg{\cdot}kg^{-1}$ than the Control, $250mg{\cdot}kg^{-1}$, $500mg{\cdot}kg^{-1}$, but the relative leaf width decreased compared to the Control, $Zn_{100}$, $Zn_{250}$, and $Zn_{500}$. The total leaf number, dead leaf number, new leaf number, and ornamental value showed the lowest value in plants grown in $Zn_{500}$ treatment but no significant differences were found among other treatments.

• Journal of fish pathology
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• v.4 no.1
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• pp.31-39
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• 1991

Vibrio sp. S-1-2 was isolated from seawater in the Masan bay and its bacterial characteristics and bioaccumulation of $CdCl_2$ in the cell were investigated. As the result of microscopic and biochemical test, the S-1-2 strain was identified to Vibrio sp. and this strain can be tolerated even in 200 ppm $CdCl_2$ media, however its growth was inhibited. In 25 ppm $ZnCl_2$ media, the growth of Cd resistant Vibrio sp. S-1-2 was promoted at later stage of growth. The growth of Vibrio sp. S-1-2 was inhibited on 25 ppm $CuCl_2$ and $PbCl_2$ media and was not able to grow in 25 ppm $HgCl_2$ media at all. The uptake of cadium in the cells was increased exponentially with increasing concentration of $CdCl_2$ in media. But the uptake rate of cadmium was suddenly inhibited at 50 ppm $CdCl_2$ media. Optimal pH and NaCl concentration for bioaccumulation of $CdCl_2$ were 8-9 and 1-2%, respectively. In the case of pH, maximum dpm value was found at pH 7 after 96 hours culture and in the case of NaCl concentration, it was detected in 2% NaCl media after 36 hours culture.

• Journal of The Korean Society of Grassland and Forage Science
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• v.27 no.2
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• pp.109-116
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• 2007

Environmental stress is the major limiting factor in plant productivity. As an effort to solve the global food and environmental problems using the plant biotechnology, we have developed transgenic tall fescue (Festuca arundinacea Schreb.) plants via Agrobacterium-mediated gene transfer method. To develop transgenic tall fescue plants with enhanced tolerance to the environmental stresses, both CuZn superoxide dismutase (CuZnSOD) and ascorbate peroxidase (APX) genes were incorporated in a pIG121 binary vector and the both of the genes were controlled separately by an oxidative stress-inducible sweet potato peroxidase 2 (SWPA2) premoter expressed in chloroplasts. Leaf discs of transgenic plants showed 10-30% less damage compared to the wild-type when they exposed to a wide range of environmental stresses including methyl viologen (MV), $H_2O_2$ and heavy metals. In addition, when $200{\mu}M$ MV was sprayed onto the whole plants, transgenic plants showed a significant reduction of visible damage compared to wild-type plants that were almost damaged. These results suggest that over expression of CuZnSOD and APX genes in transgenic plants might be a useful strategy to protect the crops against a wide range of environmental stresses.

• Journal of the Korean Society of Food Science and Nutrition
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• v.8 no.1
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• pp.25-30
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• 1979

A bacterial strain which showed a remarkable tolerance against cadmium was isolated from waste water and identified as a member of Staphylococcus aureus. Heavy metal ions, at the concentration of 10 ppm and other than cadmium, inhibited at least by 30 per cent of turbidometric growth of the organism. The organism exhibited a normal pattern of growth with increasing concentration of cadmium up to 50 ppm. However, they were unable to grow in the concomitant presence of cadmium higher than 500 ppm. In spite of the drastic growth inhibition of cadmium, the organism was able to grow even in the concomitant presence of 500 ppm cadmium when it was previously cultivated for 15 hours with exposing to 10 ppm cadmium or lead.

• Journal of Korean Society of Forest Science
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• v.90 no.1
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• pp.83-89
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• 2001

This study was conducted to test the Cd accumulation and Cd-tolerance of Pisotithus tinctorius(Pt). Pt was isolated from Pinus thunbergii forest in Muan, Chonnam Province in 1997. Pt was cultured on MMN medium supplemented with $CdSO_4{\cdot}5H_2O$ at the final concentration of 0, 0.2, 0.5, 2, and $10{\mu}g/m{\ell}$ for 40 days. Growth rate and tolerance index of the fungus were measured every week, while Cd concentration, superoxide dismutase(SOD), and glutathione reductase(GR) of the fungus were analyzed at the end of the culturing, Pt showed growth reduction in vitro at $2{\mu}g/m{\ell}$ Cd in the medium and almost stopped growth at $10{\mu}g/m{\ell}$ Cd. Tolerance index of Pt decreased with increasing Cd concentration. Cd concentration of Pt was the highest at $10{\mu}g/m{\ell}$ Cd. Activities of SOD did not show significant difference between Cd concentrations, but GR of Pt increased at $0.5{\mu}g/m{\ell}$ Cd, and decreased at $2{\mu}g/m{\ell}$ Cd. Consequently Pt could be called Cd accumulator with a tolerance mechanism to Cd. Their tolerance to Cd were expressed through the higher production of antioxidants such as GR. Pt may be used for revegetation and decontamination of soil polluted by heavy metals.