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Quantification of Inhibitory Impact of Heavy Metals on the Growth of Escherichia coli  

Jo, Gyeong-Suk (Department of Environmental Science and EngiEwha Womans University)
Gu, So-Yeon (Department of Environmental Science and EngiEwha Womans University)
Kim, Ji-Yeong (Department of Environmental Science and EngiEwha Womans University)
Ryu, Hui-Uk (Department of Chemical and Environmental EngiSoongsil University)
Publication Information
Microbiology and Biotechnology Letters / v.32, no.4, 2004 , pp. 341-346 More about this Journal
Abstract
The quantification of the toxicological effects of the heavy metals such as Cu, Cd, Cr, Hg, and Zn on the growth of Escherichia coli was performed, and the variations of toxicwith exposure time were evaluated in adaptation procedure. The characteristics of growth inhibition on Escherichia coli by heavy metals were different with metal species, and critical concentration of each metal, which inhibited cell growth completely, were Cu of 3.5 mM, Zn of 2.5 mM, Cd of 1.5 mM, Cr of 1.2 mM, and Hg of 0.12 mM, respectively. The tolerance of E. coli against heavy metals, based on $EC_{50}$ values, increased in order of Cu > Zn > Cr > Cd > Hg. The slopes obtained from the relationship between ECso values and expose time corresponds to adaptability of test organisms to the toxicants. The adaptability of test organisms to the toxicants was much higher at higher slope values. Adaptability of E. coli on heavy metals increased in order to Zn > Cd > CU >> Cr> Hg.
Keywords
Heavy metal; toxicity; Escherichia coli; growth inhibition; EC50;
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