• Korean Journal of Plant Tissue Culture
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• v.26 no.4
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• pp.271-274
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• 1999

This study was undertaken to develop an efficient propagation technique for mature Betula davurica. Using aseptic materials taken from in vitro culture, the effects of media and plant growth regulators on shoot proliferation and rooting were investigated. DKW medium turned out to be the best in shoot proliferation among the media tested. Whereas axillary buds were better culture material than apical buds in proliferation of shoots, apical buds were slightly better than axillary buds on shoot elongation. Neither 1 /2 MS nor WPM medium seemed to be suitable for shoot multiplication or elongation. When the explants were cultured on 1/2 MS medium, shoot elongation was retarded by forming big callus at the base. In the case of WPM, shoots could be formed normally, but they exhibited slow growing. NAA was so effective on in vitro rooting that more than 80% rooting could be achieved on half-strength DKW medium supplemented with 1.0 mg/L NAA after 4 weeks in cultures. Ex vitro rooting using elongated shoot was also applicable to rooting and acclimatization. Rooted plantlets were successfully acclimatized in an artificial soil mixture and grew normally. The results demonstrate that efficient mass propagation of mature B. davurica can be done through tissue culture.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.28-28
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• 2020

본 연구는 식용, 목재용, 가구용 등 유용한 상수리나무의 효율적인 양묘를 위한 기초 정보를 제공하기 위하여 용기의 종류에 따른 유묘 생장을 측정하고 조사하였다. 용기별 유묘의 엽수는 15구가 18.6개로서 24구의 18.5개보다 많았고, 엽건물중은 15구가 2.41g으로서 24구의 2.24g보다 가벼웠다. 줄기길이는 15구가 33.5cm로서 24구의 34.6cm보다 짧았고, 줄기직경은 15구가 4.48mm로서 24구의 4.77mm보다 좁았으며, 줄기건물중은 15구가 1.40g으로서 24구의 1.53g보다 가벼웠다. 뿌리길이는 15구가 39.2cm로서 24구의 49.1cm보다 짧았고, 뿌리건물중은 15구가 7.60g으로서 24구의 6.92g보다 무거웠다. 그리고 식물체건물중은 15구가 11.4g으로서 24구의 10.7g보다 무거웠다. 지상부와 지하부 (S/R) 비율은 15구가 0.50으로서 24구의 0.55보다 낮았다. 이와 같이 재배 용기의 크기에 따른 상수리나무 유묘의 생장은 여러 생장 측정 중 뿌리길이를 제외하면 T-검정에서 차이를 나타내지 않았다. 이렇게 보면 상수리나무는 용기 크기와 유묘 생장의 상관관계가 없다고 할 수 있으나 본 실험에서 노천매장 종자의 파종상 유묘를 선별하여 이식할 때 물리적 손상이 있을 수 있어 추후 추가적인 보완 실험이 필요하였다.

• Journal of Life Science
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• v.17 no.10
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• pp.1414-1418
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• 2007

This study was to examine the accumulated concentration of lead in the organ of Arabidopsis thaliana grown in the soil added three different concentrations of lead. The accumulated concentrations of lead in the stem of plant grown in the soil added official standard concentration of lead of pollutant exhaust notified by the Ministry of Environment (1 mg/l), concentration ten times higher than the official standard concentration (10 mg/l) and concentration fifty times higher (50 mg/l) were similar to the rate of increase between three different concentrations, and increased average 24% compared with normal plant stem. The accumulated concentrations of lead in the leaf of plant grown in the soil added three different concentrations of lead were increased average 57% compared with normal plant leaf. And accumulated concentrations of lead in the leaf was no significant difference according to increase of lead concentration added in the soil as stem, the rate of increase was similar to between three different concentrations. The accumulated concentrations of lead in the root of plant grown in the soil added official standard concentration of lead of pollutant exhaust and concentration ten times higher were increased average 114% compared with normal plant root, but increased about 861% in the concentration fifty times higher than the official standard concentration. This result contrast with the data of stem and leaf. The accumulated concentration of lead in the plant body of Arabidopsis thaliana was increased according to increase of lead concentration added in the soil. Especially, the accumulated concentration of lead in the plant body grown in the concentration fifty times higher than the official standard concentration was increased about 2.6 times than normal plant. These results show that lead contaminated within the soil was more accumulated in the root than the stem or leaf, and accumulated concentrations of lead in the stem and leaf were not increased in proportion to the concentration of lead in the soil, but very increased in proportion to the concentration in the root.

• Journal of Korean Society of Forest Science
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• v.86 no.4
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• pp.430-434
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• 1997

Axillary bud explants from 3-year-old seedlings of Forsythia saxatilis N., rare and endangered species in Korea, were cultured on Murashige and Skoog's medium. The effect of various cytokinins(BAP, kinetin, and zeatin) at the different concentration(0.2, 0.5 and 1.0mg/L) was tested. Although an apparent shoot proliferation was not observed, zeatin showed slight promotional effect on normal shoot and leaf development. Both shoots and adventive roots could be induced simultaneously when the explants were cultured on the medium with kinetin, but adventive rooting was gradually reduced according as BAP and zeatin concentrations increased. Axillary shoot growth was promoted by the etiolation treatment. Shoot proliferation has been maintained more than three years with consecutive subculture. Rooted plantlets were successfully acclimatized in the artificial soil mixture and showed normal growth after transplantation into field.

• Journal of Plant Biotechnology
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• v.46 no.4
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• pp.291-296
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• 2019

This study was conducted to determine the effect of a light-emitting diode (LED) on in vitro shoot growth and rooting in teak (Tectona grandis L.). In the experiments with apical bud explants, the greatest shoot elongation (3.2 cm) occurred when they were cultured on DKW medium under 50% blue and 50% red LED mixture (BR), whereas no differences in growth were observed in different light sources (florescent light [F] or BR) or media (MS or DKW). The highest number of shoot multiplication (2.4/explant) or elongation (4.94 cm) was achieved with 0.5 or 1.0 mg/L 6-Benzyladenine (BA) treatment under BR. In addition, the best rooting rate (93.8%) or root length (1.3 cm) was recorded with 0.5 mg/L indole-3-butyric acid (IBA) treatment under BR, and the highest root induction (3.1/explant) was observed in 0.2 mg/L IBA under BR. The in vitro rooted plantlets were hardened and survived well on soil.

• Korean Journal of Plant Tissue Culture
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• v.26 no.2
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• pp.133-136
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• 1999

Different kinds of cytokinins and auxins were tested for both shoot induction and rooting in a rare species, Abeliophyllum distichum Nakai. BA in WPM medium was the most effective in shoot induction, whereas zeatin seemed to be the most suitable for shoot elongation. Kinetin, at the concentration of 2.0~5.0 mg/L showed an effect in shoot induction, but the effect was inferior to BA and zeatin. Rapid shoot elongation could be achieved when the cultures were maintained on the diffuse light condition (below 500 lux) regardless of cytokinin treatments. For in vitro rooting, IBA was investigated as the best type of auxin tested when half strength GD medium was incorporated. The frequency of rooting using the plant growth regulator and medium just mentioned above was revealed as approximately 90%. In addition, the survival rate of rooted plantlets was almost 100% in an artificial soil mixture.

• Korean Journal of Plant Resources
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• v.18 no.3
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• pp.403-409
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• 2005

Mature nodal segments of 2-year-old greenhouse stock plant were cultured on Murashige and Skoog basal medium supplemented with the different kinds and various concentrations of cytokinins to produce multiple shoots in in vitro condition. The most adventitious shoots were produced from excised ends of nodal segments. The highest average number $(24.6\;{\pm}\;4.6)$ of shoots was produced with the combination of BA 1.0mg/L and TDZ 0.1mg/L in MS medium. In addition, several shoots were formed from lenticels of bark cambium with the same treatment. These concentrations promoted high shooting capability upto $94.6\%$ and NAA was the best cytokinin among five different PGR sources.

• Journal of agriculture & life science
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• v.45 no.1
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• pp.15-20
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• 2011

The objective of this study was to develop allometric equations and stem density and biomass expansion factor for Pinus rigida stands in Muju region. The coefficient of determination of the allometric equations in independent variable (dbh) and dependent variable (biomass) was more than 95% with the exception of leaf (78%) and branch(83%). The total biomass was $102Mg\;ha^{-1}$ ($65.9 Mg\;ha^{-1}$ from stem wood, $9.5Mg\;ha^{-1}$ from stem bark, $19.6Mg\;ha^{-1}$ from branch and $7.0Mg\;ha^{-1}$ from leaf). Biomass distribution ratio of Pinus rigida stands showed the highest in stem wood with 64.6%, followed by the branch with 19.2%, stem bark with 9.3% and the leaf with 6.9%. The results indicated that the stem density $(g/cm^{3})$ and the biomass expansion factor were 0.453 and 1.344, respectively.

• Journal of Plant Biotechnology
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• v.36 no.1
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• pp.1-6
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• 2009

A study was conducted to investigate in vitro micro propagation of four potato cultivars of Daese, Jasim, Chubaek and Haryeng in MS medium and PM medium (a medium containing half concentration of $NH_4NO_3$ and $KNO_3$, two fold concentration of $CaCl_2$ as compared to MS medium). During $1{\sim}2$ weeks of culture, Daese and Jasim showed better shoot elongation on the solid MS medium than Chubaek and Haryeng whereas Chubaek and Haryeng did better shoot elongation on the solid PM medium. But no difference was observed after 4 weeks of culture. As compared to shoot elongation on the solid medium, it was delayed at early stage of culture in the liquid medium without shaking. Shoot formation ratio of potato (above 4 cm of shoot length) began to increase significantly after 1 week of culture and kept on increasing until 4 weeks. The four cultivars showed the different patterns of shoot growth in bioreactor. The PM medium with a quarter salt strength was effective for the regeneration of axillary buds as well as shoot elongation of Jasim and Chubaek. Daese showed vigorous regeneration of axillary buds in the PM medium with a half salt strength. On the other hand, Haryeng showed slower growth than the other three cultivars.

• Journal of Korean Society of Forest Science
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• v.102 no.4
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• pp.543-549
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• 2013

This study was conducted to evaluate age-specific and generalized allometric equations and biomass expansion factors (BEFs) for each tree component across three age-sequence stands (35-year-old, 51-year-old, 62-year-old) of black pine (Pinus thunbergii Parl.) in Jinju, located in the western part of Gyeongnam province, Korea. Biomass in each tree component, i.e. foliage, branch, and stem, was quantified by destructive tree harvesting. Allometric regression equations were significant (P<0.05) with diameter at breast height (DBH) or combination of DBH and height ($DBH^2H$) accounting for 55-98% of the variation (as indicated by coefficients of determination, $R^2$) in aboveground biomass except for foliage biomass of the 62-year-old stand. Generalized allometric equations can be used to estimate the biomass of black pine stands because the slopes of age-specific equations over 35-year-old stands were not significantly different by the age-sequence. The stem density and biomass expansion factor (BEFs) were not significantly different (P>0.05) from different stand ages and ranged from 0.45 to $0.51gcm^{-3}$, and from 1.32 to 1.38, respectively. The results indicate that allometric equations, stem density and aboveground BEFs in the matured black pine over 35-year-old are little influenced by different stand ages.