• Title/Summary/Keyword: 주사현미경

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Application of Scanning Electron Microscopy (SEM) for Biotically Induced Microstructure Observation in Sedimentary Sample of Natural Condition (주사전자현미경 분석을 활용한 자연환경 퇴적시료의 생물기원구조 관찰)

  • Park, Hanbeom;Kim, Jinwook
    • Korean Journal of Mineralogy and Petrology
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    • v.33 no.3
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    • pp.165-173
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    • 2020
  • The activity of living microorganism directly or indirectly affects to the biomineralization in sediments and rocks that display the unique biotic structure. Minerals in the biotic structures showed unique properties and bypass the thermodynamic and kinetic barriers. Therefore, investigations on the biotically induced microstructure is essential to identify the new mineral formation mechanism by analyzing crystal structures and morphology at a nano-scale. The significant implication as well as advantages of using scanning electron microscopy to characterize the biotic structures were discussed in this paper for the examples of hydrothermal vent area microbial mat and deep-sea ferromanganese crust sample.

Carbon tip growth by electron beam deposition (전자빔 조사에 의한 탄소상 탐침의 성장)

  • 김성현;최영진
    • Journal of the Korean Vacuum Society
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    • v.12 no.2
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    • pp.144-149
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    • 2003
  • Carbon tips were grown on Si cantilevers by applying an electron beam to them directly with Scanning Electron Microscope. A carbon tip was fabricated by aligning the electron beam directly down the vertical axis of Si cantilever and then irradiating a single spot on the cantilever for a proper time in the dominant atmosphere of residual gases generated by the oil of the diffusion pump. A number of control parameters for SEM, including exposure time, acceleration voltage, emission current, and beam probe current, were allowed to make various aspect ratio feature. The growth of carbon tips was not affected by the surface morphology of substrates. We could acquired the tip whose effective length is 0.5 $\mu\textrm{m}$, bottom diameter is 90 nm and cone half angle $3.5^{\circ}$ The growth technique of the high aspect ratio carbon tips on the tip-free cantilevers is available to reduce the complexities of fabricating sub-micron scale tips on the PZT thin film actuator integrated AFM cantilevers.

STM Study of Low Dimensional Nanostructures Formed by Adsorption of Dipyrromethane-trimer Molecules on Graphite Surface (흑연 표면에 형성된 dipyrromethene-trimer 분자의 저차원 나노구조의 주사 터널링 현미경 연구)

  • Son, S.B.;Lee, S.J.;Hahn, J.R.;Shin, J.Y.;Dolphin, D.
    • Journal of the Korean Vacuum Society
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    • v.17 no.5
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    • pp.375-380
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    • 2008
  • We have investigated the low-dimensional nanostructures produced by adsorption of triangular Co coplexed dipyrromethane(DPM-trimer, Fig. 1) on graphite surface by using scanning tunneling microscope. DPM-trimer deposition on the graphite surface leads to the formation of long 1-D molecular wires and 2-D hexagonal patterns. We analyzed the heights and structures of 1-D molecular wires and 2-D hexagonal patterns. The 1-D molecular wires were formed 'edge-on' alignments on graphite surface result of continuos $\pi-\pi$ stacking interactions. The other case of 2-D hexagonal patterns were formed 'face-on' alignments on graphite surface.

Change of Epicuticular Waxes by Formulation of Fungicides in 'Campbell Early' Grapes (살균제 제형에 따른 포도의 과분 변화)

  • Shin, Kyoung-Hee;Park, Hee-Seung
    • Horticultural Science & Technology
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    • v.28 no.5
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    • pp.762-767
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    • 2010
  • This study was conducted to investigate the influence of formulation of fungicides on epicuticular waxes in 'Campbell Early' grapes. Non-bagged and bagged berries were treated with wettable powder, suspension concentrate and emulsifiable concentrate 15 days after full bloom (DAFB) and 48 DAFB (veraison), respectively. Fruit skin was observed by naked eye and scanning electron microscope (SEM) at harvest. Remarkable white blots were observed on the berries treated 48 DAFB in the order of wettable powder, suspension concentrate, and emulsifiable concentrate. The observation by SEM showed all formulations of fungicides damaged the ultra-structure of epicuticular waxes; also, an unknown substance was observed on treatment with wettable powder and suspension concentrate. Moreover epicuticular wax appeared to be melting on the grapes treated with emulsifiable concentrate. Also, non-bagged grapes showed serious contamination of epicuticular wax by some unknown substance, but bagged grapes had only fungicides' traces.

In vitro dffect of praziquantel on Paragonimus westermani by light and scanning electron microscopic observation (폐흡충에 대한 Praziquantel의 시험관내 작용에 관한 광학 및 연사전자현미경적 관찰)

  • 이순형;박호진
    • Parasites, Hosts and Diseases
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    • v.25 no.1
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    • pp.24-36
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    • 1987
  • The effect of praziquantel on P. westermani exposed in vitro was observed by stereomicroscope, light microscope and scanning electron microscope. Following results were found. 1. The worms incubated in $0.01{\;}{\mu}g/ml$ praziquantel were moving after 26-hour incubation. However, all of them were immobilized immediately after incubation in solutions over $0.01{\;}{\mu}g/ml$ concentration. 2. All of the exposed worms showed severe vacuolization not only in tegument but in subtegument, intestine, ovary, testis, Mehlis' gland and excretory bladder. 3. Vacuoles in tegument burst out to form craters. As incubation time went on, tegumental structure was disintegrated severely. The worms exposed to praziquantel were observed to be immobilized and be vacuolized of all tissues. Disintegration of reproductive organs suggests that praziquantel have suppressive effect on egg production when the flukes are not killed. The drug effects were found more related with incubation time than with drug concentration.

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