• Korean journal of applied entomology
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• v.48 no.2
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• pp.123-158
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• 2009

An attempt was made to stimulate future research by providing exemplary information, which would integrate published knowledge to solve specific pest problem caused by resistance. This review was directed to find a way for delaying resistance development with consideration of chemical(s) nature, of mixture, rotation, or mosaics, and of insecticide(s) compatible with the biological agents in integrated pest management (IPM). The application frequency, related to the resistance development, was influenced by insecticide activity from potentiation, residual period, and the vulnerability to resistance development of chemical, with secondary pest. Chemical affected feeding, locomotion, flight, mating, and predator avoidance. Insecticides with negative cross-resistance by the difference of target sites and mode of action would be adapted to mixture, rotation and mosaic. Mixtures for delaying resistance depend on each component killing very high percentage of the insects, considering allele dominance, cross-resistance, and immigration and fitness disadvantage. Potential disadvantages associated with mixtures include disruption of biological control, resistance in secondary pests, selecting very resistant population, and extending cross-resistance range. The rotation would use insecticides in high and low doses, or with different metabolic mechanisms. Mosaic apply insecticides to the different sectors of a grid for highly mobile insects, spray unrelated insecticides to sedentary aphids in different areas, or mix plots of insecticide-treated and untreated rows. On the evolution of pest resistance, selectivity and resistance of parasitoids and predator decreased the number of generations in which pesticide treatment is required and they could be complementary to refuges from pesticides To enhance the viability of parasitoids, the terms on the insecticides selectivity and factors affecting to the selectivity in field were examined. For establishment of resistant parasitoid, migration, survivorship, refuge, alternative pesticides were considered. To use parasitoids under the pressure of pesticides, resistant or tolerant parasitoids were tested, collected, and/or selected. A parasitoid parasitized more successfully in the susceptible host than the resistant. Factors affecting to selective toxicity of predator are mixing mineral oil, application method, insecticide contaminated prey, trait of individual insecticide, sub-lethal doses, and the developmental stage of predators. To improve the predator/prey ratio in field, application time, method, and formulation of pesticide, reducing dose rate, using mulches and weeds, multicropping and managing of surroundings are suggested. Plant resistance, predator activity, selective insect growth regulator, and alternative prey positively contributed to the increase of the ratio. Using selective insecticides or insecticide resistant predator controlled its phytophagous prey mites, kept them below an economic level, increased yield, and reduced the spray number and fruits damaged.

• Korean Journal of Microbiology
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• v.49 no.1
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• pp.64-70
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• 2013

The greenhouse whitefly, Trialeurodes vaporariorum, is an economically important pest for greenhouse crops because they cause direct damage by feeding on plant nutrients and indirect damage as transmits many virus vectors. It has recently become a serious problem because of the continuous use of insecticide resulting in resistance among greenhouse whitefly population. To overcome these problems, in this study, the biological characteristics and virulence of an entomopathogenic fungus isolated from the cadaver of nymph greenhouse whitefly were investigated. Isolated fungus was identified as Isaria fumosorosea by morphological examinations and genetic identification using sequences of the ITS, ${\beta}$-tubulin, and EF1-${\alpha}$ regions. This fungus was named as I. fumosorosea SDTv and tested for the virulence against nymphs T. vaporariorum and the cold activity, the thermotolerance and the stability of UV-B irradiation on conidia. Mortality rate of greenhouse whitefly showed from 84 to 100% and the virulence increased with increasing conidial concentrations, $1{\times}10^5$ to $10^8$ conidia/ml. Conidia were stable at $35^{\circ}C$, 0.1 $J/cm^2$ of UV irradiation and germinated after 8 days at $4^{\circ}C$. Additionally, the activities of chitinases and proteases produced by I. fumosorosea SDTv were varied according to the medium. In conclusion, I. fumosorosea SDTv which showed high mortality rate against greenhouse whitefly will be used effectively in the integrated pest management programs against the greenhouse whitefly.

• Journal of Applied Biological Chemistry
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• v.54 no.4
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• pp.290-295
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• 2011

This study was carried out to investigate the quality of soil, irrigation water, and status of agrochemicals application in relation to good agriculture practices system in the major cultivation area of Disocorea batatas. The concentrations of heavy metals as Cd, Pb, Cu, and Zn in soils and irrigation waters were lower than those of standard level for Environmental Conservation Act of Soil and Water in Korea. The dominant weed have been appeared Digitaria sanguinalis and Portulaca oleraceamite. The dominant insect pests and plant pathogens have been appeared aphid and anthracnose. Average yearly application of pesticide was 2 to 4 times for herbicide and 4 to 6 times for plant pathogens and insect pests. In order to safety production of medicinal crops could be achieved by proper cultivation management such as minimum application of agro-chemicals, effective use of by-product fertilizer, and technology development of organic farming.

• The Korean Journal of Pesticide Science
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• v.13 no.2
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• pp.111-116
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• 2009

Juvenile hormone (JH) is an insect hormone mediating immature metamorphosis and adult reproduction. It also mediates immune responses to suppress hemocyte behavior, which is, however, activated by ecdysteroid. This study investigated an effect of a commercial pyriproxyfen (a JH agonist) formulation on a cellular immune response of the diamondback moth, Plutella xylostella, and analysed its mixture with Bacillus thuringiensis (Bt) in insecticidal potency. The commercial pyriproxyfen formulation significantly suppressed hemocyte-spreading behavior at low doses as did in pyriproxyfen technical grade. When the commercial pyriproxyfen formulation was mixed with Bt, Bt toxicity was significantly increased against P. xylostella larvae in laboratory. The mixture effect was then confirmed in field cultivating cabbage infested with P. xylostella larvae. The mixture showed a significantly enhanced mortality and reduced effective lethal time, compared to only Bt treatment.

• Korean Journal of Agricultural Science
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• v.13 no.2
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• pp.176-184
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• 1986

A biological study was done on primary parasites and hyperparasites of green peach aphid, Myzus persicae, collected in Taejeon area during the period from April to September 1985 was. The results of mummification of primary parasites, host selectivity between primary parasites and hyperparasites, and longevity of important species were obtained as follows; 1. Among the 201 mummies collected in the field, adult primary parasites and adult hyperparasites were 38.3% and 44.3%, respectively. 2. Among the primary parasites, Lysiphlebia japonica and Trioxys hokkaidensis were most important species. 3. Among the hyperparasites, Prataphelinus nikalskajae, Eucoila sp. and Ardiles convexa were dominant species. 4. L. japonica was more parasitized by the hyperparasites than T. hokkaidensis. 5. T. hokkaidensis was higher than L. japonica in the ability of parasitism. 6. L. japonica had lived for 3 days by feeding glucose, the other foods had lived shorter than food.

• The Korean Journal of Pesticide Science
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• v.19 no.2
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• pp.93-100
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• 2015

This study surveyed the selling, buying, usage, selection and spraying frequency of pesticides on apple orchards in Geochang, Gyeongsangnam-do province from 2012 to 2013 and found that the fungicides, insecticides and acaricides were sprayed $13.9{\pm}3.5$, $12.6{\pm}3.2$, and $2.6{\pm}1.3$ times per year, respectively. Fungicides were applied mainly to control for Diplocarpon mali, Colletotrichum gloeosporoides and Alternaria mali, whereas insecticides were sprayed mostly to control Grapholita molesta, Carposina sasakii insects. Dealers sold pesticides without monitoring of the pests in the apple orchards, and also sometimes sold pesticides which are non-registered for apple. Most of the farmers were highly relied on dealers' recommendations to choosing the brand product. Relating on Integrated Pest Management (IPM) on apple orchards in Geochang, residual active ingredient of frequently sprayed fungicides, insecticides, and acaricides were analyzed. Most applications of the fungicides, insecticides and acaricides were well corresponded with FAO's recommendations. For production of safe food and use of pesticides, it is requested to develope control calender and consideration of training program for farmers. The regional characteristics and environmental situation of the farm also should be considered.

• Korean Journal of Organic Agriculture
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• v.7 no.2
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• pp.169-177
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• 1999

This experiment were performed on rice dignity during 1998 at paddy field of Dong-a Univ., Pusan, Korea. Depression effect of weed occurrence by pasture of 2kg of the Ampullarius insularus per 10a was 80.4% at the 7th day after transplanting, 69.7% at the 14th day after transplanting pasture plot and 51.8% at the 21th day after transplanting. Deperssion effect of weed occurrence by pasture of 5kg of the Ampullarius insularus per 10a was 97.9% at the 7th day after transplanting, 79.2% at the 14th day after transplanting pasture plot and 64.1% at the 21th day after transplanting. Depression effect of weed occurrence by pasture of 8kg of the Ampullarius insularus per 10a was 97.9% at the 7the day after transplanting, 87.1% at the 14th day after transplanting pasture plot and 71.4% at the 21th day after transplanting. Weeds ingestied by the Ampullarius insularus were in the order of perennial weed, annual weed.

• Korean Journal of Remote Sensing
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• v.25 no.5
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• pp.435-444
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• 2009

In the case of oil spill accident at sea, information concerning the movement of spilled oil is important in making response strategies. Aircrafts and the satellites have been utilized for monitoring of spilled oil. In these days, numerical models are using to predict the movement of the spilled oil. In the future a coupling method of modeling and remote sensing data should be needed to predict more correctly the spilled oil. The purpose of this paper is to present an application of satellite image data to an oil spill prediction model as an initial condition. Environmental Fluid Dynamics Computer Code (EFDC) was used to predict the movement of the oil spilled from Hebei Spirit incident occurred in Taean coastal area on December 7,2007. In order to make the model initial condition and to compare the model results, two satellite images, KOMPSAT-2 MSC and ENVISAT ASAR obtained on December 8 and 11, were used during the period of the oil spill incident. The model results showed an improvement for the prediction of the spilled oil by using the initial condition deduced from satellite image data than the initial condition specified at the oil spill incident site in the respects of the distributed spilled area.

• The Korean Journal of Pharmacology
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• v.28 no.2
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• pp.147-162
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• 1992

The objectives of the present experiments were to characterize the effects of the peptides belonging to the pancreatic polypeptide family on the contractility of cerebral arteries and to observe the interactions of these peptides with the cyclic nucleotide activators and the potassium channel openers. Dogs of either sex, $20{\sim}30\;Kg$ in weight, were sacrificed. Basilar and middle cerebral arteries from brain were isolated and prepared for myography in the PSS equilibrated with 95% $O_2$ and 5% $CO_2$ at $37^{\circ}C$. The endothelial cells of the spiral strips were removed by CHAPS solution (0.3% w/v, 15 seconds). 1. PP, PYY and NPY contracted the arterial strips concentration-dependently with a rank order of potency of PYY>NPY>PP. These peptides were 20 to 200 times more potent than norepinephrine, and only PYY showed a greater potency than 5-HT. 2. Cyclic nucleotide activators, forskolin (for cAMP) and sodium nitroprusside (for cGMP) reduced the basal tone and inhibited the PP-, PYY- and NPY- induced contractions by concentration-dependent manners. Forskolin was more potent in reducing basal tone than sodium nitroprusside. 3. Potassium channel openers, RP 49356, P 1060 and BRL 38227 reduced the basal tone concentration-dependently and tended to inhibit the PP-, PYY- and NPY- induced contractions. Notably, BRL 38227 with low concentration $(0.1\;{\mu}M)$ enhanced the contractions induced by those peptides while P 1060 inhibited the contractions concentration-dependently. 4. The combinations of the cyclic nucleotide activators and the potassium channel openers were slightly additive in reducing the basal tone. P 1060 and BRL 38227 enhanced the relaxant effect of sodium nitroprusside significantly. On the PYY-induced contration $(0.1\;{\mu}M)$, $K^+$ channel openers tended to inhibit the inhibitory actions of forskolin and sodium nitroprusside. P 1060 and BRL 38227 antagonized the inhibitory action of sodium nitroprusside significantly. The results of the present study may be summarized that in canine cerebral arteries, not only NPY but also PYY may play a role in a cerebrovascular spasm, and intracellular concentration of either cAMP or cGMP may be involved in the mechanism of vasoconstrictive actions of these peptides, which may be affected either positively or negatively by a $K^+$ channel opener.

• Weed & Turfgrass Science
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• v.4 no.3
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• pp.219-224
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• 2015

This study was conducted to evaluate the effect of herbicidin, new natural herbicidal substances, derived from soil actinomycetes Streptomyces scopuliridis. Several weed species were subjected to examine the germination inhibition and herbicidal activity at the concentration from 100 to 2,000 ppm. There was no selectivity in germination inhibition and herbicidal activity against crops. Germination of Echinochloa oryzoides, Digitaria ciliaris, Abutilon theophrasti and Amaranthus retroflexus was inhibited completely when 7.81 ppm of extract was treated in petri dish. Pre-emergence application of herbicidin in soil condition showed low inhibition against weeds. However, post application of herbicidin in green house resulted in the necrosis of weeds at the concentration of 2,000 ppm. A. retroflexus was sensitive to herbicidin at the low concentration of 62.5 ppm, whereas E. oryzoides was tolerant to lower concentration of herbicidin until it became withered at the concentration of 2,000 ppm. In conclusion, herbicidal substances derived from S. scopuliridis herbicidin, which is consisted with herbicidin A and B, have dominant effect on germination and growth inhibition. On the other hand, herbicidin was insufficient to control gramineous weeds. In future, it will be needed to develop the combination of herbicidin with other herbicide or compounds to control gramineous weeds as well.