• Journal of Life Science
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• v.20 no.1
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• pp.22-26
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• 2010

Seven rice germplasms with red pigmentation within the pericarp were isolated from a large mutant collection. These red pericarp phenotypes resulted from the functions of the Rc, Rd and RdRc genes. Among them, two brown pericarp of the Rc type, four red pericarp of the RdRc type, and one white pericarp of the Rd type were identified. Morphological and agronomic characteristics of those rice germplasms were studied. The Rc type germplasms have the faint red or brown color pericarp and the Rd types produce the white pericarp, whereas the RdRc type germplasms have the dark red pericarp. Most of the important agronomic characteristics including plant stature, tillering ability, spikelet fertility, and total grain yield were lower in the colored rice than those of the wild-type control. All of the studied colored rice germplasms had a tendency of easy seed-shattering in comparison to the control. These characteristics of newly identified germplasms will be useful for identifying the genes responsible for pericarp color phenotype determination.

• Current Research on Agriculture and Life Sciences
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• v.3
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• pp.8-13
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• 1985

Egremont russet 사과 품종(品種)의 종자에 여러가지 종피(種皮) 파상(破傷)을 한 후 발아(發芽), 유아(幼芽), 유근(幼根)의 생장(生長)과 생리적(生理的)인 왜화(矮花)에 대(對)하여 조사한 결과 다음과 같은 결과를 얻었다. 자엽(子葉)의 크기를 1/2로 절단하고 종피(種皮)를 제거(除去)한 Egremont russet 사과 품종(品種)의 종자(種子)에 $GA_3$를 처리한 것은 평균발아율(平均發芽率)이 55%였으며 동일조건(同一條件)에서 대조구(對照區)는 45%였다. 그리고 종피(種皮)가 완전(完全)한 것과 종피파상(種皮破傷)을 한 구(區)에서는 낮았다. 유아장(幼芽長)은 종피(種皮)를 완전히 남긴 것과 파상(破傷)한 구(區)에 $GA_3$를 처리하였을 때 촉진(促進)되었으나 전체적(全體的)으로 볼 때 종피(種皮)를 제거(除去)한 구(區)에서 더 길었다. 그러나 유근장(幼根長)은 $GA_3$처리에 의(依)해서 억제(抑制)되었으나 유근(幼根)의 직경(直徑)은 더 커졌다. 그리고 생리적(生理的)인 왜화(矮花)는 종피(種皮)를 남긴 구(區)에서 발생(發生)되었으며 $GA_3$ 처리에 의(依)해 극복되지 않았다. 자엽(子葉)의 녹색화(綠色化) 속도는 종피(種皮)를 제거(除去)하고 자엽(子葉)의 크기를 1/2로 절단한 구(區)에서 촉진(促進)되었다.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.spc1
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• pp.123-131
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• 2005

Lipid and protein contents in whole soybean seeds have negative correlation (r=-0.693**), however, these components in seed coat showed positive correlation (r=0.746**). Fatty acids in whole soybean seeds were higher in the order of $C_{18:2}>C_{18:1}>C_{16:0}>C_{18:3}>C_{18:0}$, while those of seed coat were higher in the order of $C_{18:3}>C_{18:2}>C_{18:0}>C_{16:0}>C_{18:1}$. The average content of total amino acid in twenty Korean soybean varieties was 38,938.7 mg/100 g, while that of seed coat was 4,418.4 mg/100g. Glutamic acid showed the highest composition rate $(16.4\%)$ in whole soybean seeds, while glycine was the highest in seed coat and their composition rate was $23.8\%$. The surface of shiny-lustre seed coats was smooth and their pore size was observed smaller than dull-lustre ones. Significant quadratic regression was observed among seed coat lightness, seed coat thickness, protein, lipid, unsaturated fatty acid and crude fiber. Fucose, rhamnose, glucose, mannose, galactose, arabinose and xylose were detected as a neutral mono-saccharides in the seed coats. The arabinose and xylose showed significant correlation with seed coat lightness. The unsaturated fatty acid was significantly correlated with seed coat lightness (r=0.726**). Water absorption rate was low in the thick seed coat varieties, but the rate was high in the shiny seed coat varieties. From the obtained results, it was considered that the thinner and brighter seed coat varieties were much favorable to increase the water absorption rate than thicker and darker seed coat ones.

• Korean Journal of Plant Taxonomy
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• v.38 no.1
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• pp.1-16
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• 2008

Anatomical features of seed coat were examined on Trollius, Calathodes, and Caltha of Ranunculaceae to evaluate the taxonomic circumscription of Megaleranthis saniculifolia, which is monotypic and endemic in Korea. Megaleranthis saniculifolia showed the exotestal type of seed coat exhibiting a well-developed palisade structure in exotesta and its external surface of exotesta cells was concave. On the other hand, the shape of exotesta cells in Caltha was cuboidal and the outer surface was smooth. The exotesta of Calathodes seeds was formed of the palisade structure like M. saniculifolia, but the outer surface of exotesta cells was smooth. The palisade structure was much better developed in the exotesta of Calathodes as well as Megaleranthis seeds than in the exotesta of Trollius seeds. The outer surface of exotesta cells in Trollius was either convex or concave according to the species examined. Since the genera of the subtribe Calthinae of the family display differences in seed sculpturing and the anaotmy of seed coat, these characteristics are useful to access taxonomic relationships among them. The morphological and anatomical features of seed coat suggest that Megaleranthis be possibly allied with Trollius rather than Caltha or Calathodes. Concave surface of seed coat cells and well-developed palisade structure of exotesta are shared by M. saniculifolia and some species of Trollius in common.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.66 no.4
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• pp.350-357
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• 2021

In the present study, the seed color of 200 common bean genetic resources was analyzed and located on the HTML color chart to classify these resources according to color characteristics. This classification method predicts the components of seed and may serve as a new method for efficiently using secured genetic resources. The imagary data of common bean exhibiting various seed colors were expressed using the HTML color chart. According to the proposed classification method, the seed color was distributed in seven categories: yellow-green, yellow, brown, red, white, gray, and indigo. In addition, the distribution of each seed color was according to its concentration. The distribution by concentration was the highest for red, whereas the distribution of gray and yellow-green was not concentration-dependent. As the dominant pigments based on color distribution, chlorophylls in yellow-green; carotenoids in yellow; and anthocyanins in brown, red, white, gray, and indigo significantly affected seed color. When expressed objectively, seed colors can be applied to the systematic management, breeding, and cultivation of genetic resources and can be useful for marketing or developing products of desired colors. This method can also be applied to other crops.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.55 no.1
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• pp.19-23
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• 2010

This study was performed in order to determine the relationship between anthocyanin generation and seed coat pigmentation in black soybean. Soybean genotypes were analyzed the individual anthocyanin contents by UPLC, which were sampled at 5-day intervals from the 35th day after flowering. Ilpumgeomjeongkong had begun to accumulate anthocyanin on the seed coat previous 35 days after flowering, and in case Heugcheongkong was 30 days. The seed coat coloration in Ilpumgeomjeongkong run on till the 45th day after flowering, and that of Heugcheongkong was between 55 and 60days after flowering. Cyanidin-3-Glucoside (C3G) was formed the earliest and accumulated the greatest among three anthocyanin pigments existed in black soybean. So we could be concluded that C3G affected on seed coat pigmentation greatly than other pigments. The anthocyanin contents at maturity in Ilpumgeomjeongkong was 4.4 times higher than at beginning stage of anthocyanin formation, while those of Heugcheongkong was 2.5 times.

• Journal of Life Science
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• v.12 no.6
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• pp.694-699
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• 2002

The present experiment was carried out to determine the effect of seed-coat softening by various chemicals, washing, chilling, and light treatment alone and in combination during desiccation of seeds on germination of gourd (Lagenaria siceraria Standl.) seeds. Potassium hydroxide, sodium hydroxide, acetone, acetonitrile, and acetamide were used as chemicals for the softening. Washing, prechilling, drying and light treatment of the softened seeds were done for a hour with tap water, for one or three weeks at 3$^{\circ}C$, 12 hr at 35$^{\circ}C$, and 14 hr a day at 3$0^{\circ}C$ with red light illumination or darkness, respectively. FR-yongjadaemok and FR-kunghap were used as test cultivars. Seed-coat softening with 10% acetone and acetonitrile for 1 hour enhanced and accelerated seed germination compared to the other chemicals and water imbibition. KOH treated for the softening was necessary to increase the germination rate of seeds for following chilling, but acetone did not influence their germinability regardless of chilling. Washing the softened seeds in tap water increased the germination rate in comparison with no-washing. During desiccation of the seeds sequentially treated with the softening, washing and chilling, red light treatment showed higher germination rate than dark one, suggesting that red light treatment are necessary during desiccation.

• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.8
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• pp.1150-1156
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• 2015

The objective of this study was to investigate the polyphenolic compounds found in different parts of red and black adzuki beans and to determine the contribution of polyphenolic compounds to the antioxidant properties of adzuki beans. Total polyphenolic and proanthocyanidin contents were studied and their antioxidant activities were determined by ferric reducing antioxidant power, 1,1-diphenyl-2-picrylhydrazyl (DPPH), and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt assay. The highest total polyphenolic content was found in seed coats (26.1~33.9 mg/g), followed by whole beans (6.9~8.0 mg/g) and dehulled beans (3.3~3.4 mg/g). The highest total proanthocyanidin content was also found in seed coat with 26.5~30.7 mg/g. Moreover, seed coats exhibited the highest antioxidant activity regardless of analytical methods. Antioxidant activity was positively and significantly correlated with total polyphenolic content with the exception of dehulled beans, in which there was no correlation with total polyphenolic content. In particular, the highest correlation was found between DPPH and total polyphenolic content (r=0.945, P<0.01) in whole beans.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.3
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• pp.169-172
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• 2003

Intact seeds of burcucumber (Sicyos angulatus L.) are not nearly germinated so that the farmers remove their arils before sowing. The study was carried out to measure the effort of aging, chilling, washing, chemical treatment, drying, and their sequential treatments on the germination of ones with arils and the seedling emergence. The seeds extruding over 1mm of their radicles and the seedlings with fully expanded cotyledons were counted daily for calculating the germination and the seedling emergence rates. Seedcoat softening using 10% NaOH solution somewhat enhanced their germination rate of the intact seeds harvested in the current year, but aging treatment done on 6 days at $45^{\circ}C$ after NaOH treatment increased the germination rate more than only NaOH treatment. A day washing after the NaOH treatment and a day chilling after the washing increasingly elevated the rates as well. Irrigation of 0.2% $\textrm{KNO}_3$ solution, moreover, showed the greatest germination rate of the priming treatments done during all the 7 day chilling. Drying the seeds after the combined treatment of chilling and priming more inclined the germination rate than nondrying ones, but red light treated during 24 hour desiccation was more effective in the germination rate increment than dark condition. The seeds done the sequential treatments of aging, NaOH, washing, combining chilling and priming and drying under red light illumination were germinated up to 80% but seedlings were emerged to 60%, lower than ones removed their arils.

• Korean Journal of Plant Taxonomy
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• v.42 no.4
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• pp.247-252
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• 2012

Seed morphology in the subtribe Agrimoniinae (Rosaceae) was examined using scanning electron microscopy to identify distinct characters and evaluate their evolution in a phylogenetic framework for five genera in the subtribe: Agrimonia L., Aremonia Neck. ex Nestl., Hagenia J.F. Gmel., Leucosidea Eckl. & Zeyh., and Spenceria Trimen. All genera have one or two mature achenes in a fruiting hypanthium. In the seed coats, the cell shape, size, wall features, and sculpturing vary across genera. Of most significance is the presence of papillae structures in both Agrimonia and Aremonia. Through the mapping of papillae features onto phylogenetic trees, either one or two changes in seed coats are hypothesized. The phylogenetic tree inferred from four nuclear and six chloroplast regions of sequence data suggests that at least two steps of papillae sculpturing on seed coats are required. On the other hand, in the phylogenetic tree of a low-copy nuclear gene, one independent evolutionary step is postulated to explain the current character states. In the latter hypothesis, the seed coat sculpturing also supports a monophyletic relationship for cosmopolitan Agrimonia and European endemic Aremonia. The seed coat sculpturing provides valuable information for inferring phylogenetic relationships at the generic level in the subtribe Agrimoniinae.