• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.10-10
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• 2023

투구꽃속(Aconitum L.)은 미나리아재비과(Ranunculaceae)의 가장 큰 속(Genus) 중 하나로 300-400 여종(Species)이 북반구 온대지역에 분포한다(Tamura, 1993). 둥근 투구모양의 꽃받침이 특징적이며, aconitine과 같은 알칼로이드 성분을 함유하고 있어 전통적으로 약용소재로서 활용되었다(Khorana, 1968). 세뿔투구꽃(Aconitum austrokoreense Koidz.)은 한국 경상도, 전라남도에만 국지적으로 자라는 한국 특산식물이자, IUCN에서 CR(Critical Endangered) 등급으로 지정된 희귀식물이다. 이 종은 다년생이지만, 서식지에서 개체수가 적거나 유묘의 발견이 어려운 등 자생지 축소 및 절멸의 우려가 높다. 세뿔투구꽃을 보호하고 종을 보존하기 위해서는 종자 발아 · 휴면에 대한 지속적 연구와 종자번식법의 개발이 필요하다. 본 연구에서는 세뿔투구꽃의 종자의 휴면유형을 분석하고 호르몬에 의해 휴면타파를 유도하였으며, 특히 발아 2단계에서 일어나는 생리적 변화를 단백질체학적 관점에서 분석하였다. 세뿔투구꽃 종자는 미발달된 배를 가지고 있으며, 충분한 수분과 온도의 조건에서도 발아가 관찰되지 않아 형태 · 생리적 휴면으로 판단되었다. 종자는 BAP 처리에서 휴면이 타파되는 것을 확인하였으며, 특히 BAP 농도에 따른 발아율은 통계적으로 유의한 값을 가졌다 (F=23.208, P<0.01). 건조된 종자에 비해 BAP 처리된 종자는 발아 2단계에서 몇가지 단백질의 증가가 관찰되었다. 대부분의 종자는 30S ribosomal protein subunit으로, 휴면상태에서 발아상태로 변환이 일루어지는 생리적 변화의 증거로 확인된다. 특히 발아2단계를 지나는 세뿔투구꽃 종자에서는 cytochrome subunit과 photosystem II protein의 급격한 증가가 관찰되었는데, 이는 배아의 발달 결과인 자엽이 발아 즉시 광합성이 가능하도록 준비하는 과정에서 발현 된 것으로 추정한다. 본 연구는 발아2단계에서 휴면타파에 직접적인 영향을 미치는 요인을 발견하지 못하였으나, 휴면타파에 중요한 단계인 발아 2단계 종자의 생리학적 변화를 이해하기 위한 기초자료로 활용 될 수 있을 것이다.

• Journal of Korean Society of Forest Science
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• v.95 no.3
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• pp.316-322
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• 2006

This study was conducted to examine the changes of seed characteristics and germination properties and to determine the most suitable collection time in Ulmus davidiana var. japonica. Seeds were collected six times with the interval of seven days after April 21, 2005 at Jeong-seon. There were differences in seed characteristics and germination properties among seed collection times. The sizes of seed and fruit increased with the collection time, but seed moisture contents decreased with the collection time after increasing until May 5. The 1,000 seeds weight decreased with the collection time after increasing until May 12. Collection time showed high positive correlations (r=0.790, p<0.01) with seed size and negative correlations (r=-0.919, p<0.01) with moisture contents. Percent germination (PG) presented the highest value in seeds collected at May 5, and decreased with the collection time. Mean germination time(MGT), germination speed(GS), and germination performance index(GPI) also showed the highest values in seeds collected at May 5. PG, MGT, and GS had correlations with other seed characteristics except the 1,000 seed weight. The relative growth rate of height and root collar diameter of seedlings presented the highest values in seedling germinated from seed collected at May 5, and seedlings germinated from seeds collected at May 5 and 12 showed the highest values in chlorophyll contents. Consequently it will be possible to product a number of seedlings when the seeds of U. davidiana var. japonica are collected just before drying morphologically.

• 한국양묘협회지
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• s.10
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• pp.47-52
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• 1982

• Korean Journal of Medicinal Crop Science
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• v.4 no.3
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• pp.193-198
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• 1996

To establish appropriate seed production system in Angelica gigas. Germination of seeds with different bolting years and the growth characters of plants originated from these seeds were in­vestigated. Seed weight from 1st year bolting plant was highest. In germinability according to seeds with different bolting years, seeds from 1st year bolting plant were higher than seeds from 2nd, 3rd years bolt­ing one. In growth characters of plants originated from seeds with different bolting years, as the bolting years became late, root growth increased and top growth decreased, therefore T/R ratio decreased.

• Korean Journal of Medicinal Crop Science
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• v.4 no.2
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• pp.163-171
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• 1996

The influences of temperature, harvest time, sowing time, seeding media, moisture content of media and light on the seed germination of Lycoris are as follows, Both L. koreana and L. aurea had over 90% seed setting, and seed maturity came in September or early October. It took six months to have over 60% seed germination. The optimum germination temperature was $25^{\circ}C$, and the alternating treatment was effective at $20{\rightleftarrows}25^{\circ}C$. The optimum harvest-time was around Sep. 20, and the optimum seed-time was immediately after havest. The optimum seeding media was sand loam, and the optimum moisture content was PF 1. 9/400ml seeding media. The dark condition was more effective for the seed germination than the light condition.

• Korean Journal of Plant Resources
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• v.26 no.1
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• pp.60-67
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• 2013

A laboratory experiment was conducted to determine the content of phenolics and flavonoids, antioxidant activity and antioxidant enzyme activity for the extracts from cowpea seed and sprouts. Plant length and weight of cowpea sprouts were significantly increased until 7 days after seeding. Total phenolics level [mg chlorogenic acid equivalents (CAE) $kg^{-1}$ DW] was highest in dry seed (DS) extracts of cowpea ($63.9mg\;kg^{-1}$), followed by imbibed seed (IS) ($56.8mg\;kg^{-1}$) and 1-day-old sprout (1DOS) extracts ($46.4mg\;kg^{-1}$), and significantly reduced with increase of sprout age (p < 0.05). The antioxidant activity of the methanol extracts from all the samples showed same tendency to the results of total phenolics level, and dose-dependently increased. DPPH (1,1-diphenyl-2-picryl hydrazyl radical) free radical scavenging activity was higher in DS (87.3%) and IS (41.2%) than in cowpea sprouts from 1DOS to 7DOS, ranging from 17.1 to 30.4%. Antioxidant enzymes, APX, POX, and POX activities were highest in 7DOS and lowest in DS. SOD activity showed much higher activity in sprouts and in seeds. Correlation coefficient between physiological-active substance and the activity was highest between APX and CAT activities ($r^2$= 0.9574). Especially, total phenolics content was more highly correlated with antioxidant or with antioxidant enzyme activities than was total flavonoid level.

• Journal of Korean Society of Forest Science
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• v.96 no.3
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• pp.317-324
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• 2007

This study was carried out to obtain the several data for improving the seed supply in Pinus densiflora. Seed characteristics and germination properties according to change of cone production from 2003 to 2006 were analyzed in three plus stands of P. densiflora. P. densiflora showed good production at interval of two years in the periodicity of seed production. Seed potential and percent of filled seed (PFS) among the major seed characteristics showed positive relations to the change of cone production, whereas percent of developed seed (PDS) and seed efficiency showed opposite trend. As a result of regression analysis between seed characteristics and cone production, seed potential ($R^2=0.682$, p<0.01) and PFS ($R^2=0.161$, non-significant) presented positive correlation with cone production while PDS ($R^2=0.418$, p$R^2=0.308$, non-significant) presented negative correlation. In germination properties percent of germination (PG) showed similar tendency to cone production, while mean germination time (MGT) showed opposite tendency. As a result of regression analysis between germination characteristics and cone production, PG showed positive correlation, whereas MGT showed negative correlation. Therefore, we could expect high germination percentage and short period for germination when we harvested cones at good cone production time.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.67-67
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• 2022

먹넌출은 국내에서 안면도에서만 자생하는 희귀식물로, 항산화, 미백, 항암 효능이 밝혀져 유용식물자원으로의 활용이 기대되나 IUCN(세계자연보전연맹) 기준 취약종(VU)으로 분류되어 생명자원의 현지 내, 현지외 보존 필요성이 크다. 따라서 본 연구는 먹넌출의 종자를 이용한 보존과 증식을 위해 기초자료를 확보하고자 하였다. 이를 위해 열매의 성숙도에 따른 형태적 특성과 활력 차이를 비교하고, 종자의 발아 조건 구명을 위해 휴면 종류와 발아 적온을 조사하였다. 먼저 열매 성숙도에 따른 형태적 특성과 활력 조사 결과, 열매가 부분적으로 성숙한 적색 단계는 완전히 성숙한 흑색 단계와 비교하여 종자 무게와 배길이에서 차이가 거의 나타나지 않았고 충실도가 80%, 충실 종자의 활력이 100%로 나타났다. 따라서 종자 채취 시 열매가 흑색으로 성숙하여 떨어지기 전인 적색 단계에서 채취하여도 종자를 이용한 증식에 문제가 없을 것으로 보인다. 또한 먹넌출 종자는 딱딱한 과피로 덮여있지만 수분 흡수를 가능하게 하는 조직을 가지고 있어 물리적 휴면에 해당하지 않았으나 생리적 휴면을 가지고 있었으며, 휴면 타파에는 GA3 1000ppm 처리가 가장 효과적이었다. 또한 발아 온도는 10℃ 12시간, 20℃ 12시간인 변온 조건에서 가장 높은 발아율을 보였다. 본 결과를 먹넌출 종자 수집과 증식에 참고 자료로 활용할 수 있을 것으로 기대한다.

• Korean Journal of Weed Science
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• v.11 no.3
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• pp.219-223
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• 1991

Effects of media and low temperature on germination of weeds were examined to get basic information for establishing weed control methods. Soil and agar(0.4%) was good media for germination of weeds at laboratory. Germination was improved by placing weed seeds at 5$^{\circ}C$ low temperature for 10-20 days. Germination was improved by storing weed seeds in water absorbed gauge and in vinylbag at 5$^{\circ}C$ low temperature for 30-40 days. Germination was increased by burial of weed seeds at 10cm of soil depth for 30-60 days.

• Current Research on Agriculture and Life Sciences
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• v.9
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• pp.135-146
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• 1991

This study was attempted to know the effect of crude extracts of Allium fistulosum on the seed germination and seedling growth in compositae. The result is as follows; The seed germination and seedling growth in the tested compositae were inhibited by the crude extracts from leaf blade, leaf sheath and root of Allium fistulosum. Generally the crude extracts from leaf blade part showed the highest inhibitory effect in the tested parts. The seed germination was inhibited by the crude extracts of Allium fistulosum extracted with 80% methyl alcohol and distilled water. But the crude extracts which had extracted with distilled water indicated more inhibitory effect than that of 80% methyl alcohol. The seedling growth was depressed by the root residues of Allium fistulosum in the soil after harvesting. The crude extracts had the character of stability in light and heat. Some out of the inhibitors contained the volatile substances.