• Korean Journal of Plant Resources
• /
• v.24 no.5
• /
• pp.628-635
• /
• 2011

This study was conducted to establish the tissue culture system for Korean domestic Miscanthus sinensis, which is used in various purposes such as forage, and bio-energy resources. With the mature seed of Miscanthus, optimum concentrations of plant growth regulators were identified for an efficient callus induction and regeneration. Among the treatments of 1~10 $mg{\cdot}L^{-1}$ 2,4-D, IBA, or NAA, callus induction rate was highest (85.3%) on MS medium containing 5 $mg{\cdot}L^{-1}$ 2,4-D. Under the condition, the callus were efficiently induced and proliferated with comparably lower frequencies of callus browning. In shoot regeneration, the treatment of NAA combined with BAP seemed to contribute more efficient conditions to shoot regeneration than those of NAA with Kinetin or 2-iP. Especially, regeneration efficiency and number of regenerated plants were 83.7% and 5.5 in 3 $mg{\cdot}L^{-1}$ NAA with 5 $mg{\cdot}L^{-1}$ BAP, respectively, which were higher frequencies than those in NAA with Kinetin or 2-iP. In results, 5 $mg{\cdot}L^{-1}$ 2,4-D and 3 $mg{\cdot}L^{-1}$ NAA combined with 5 $mg{\cdot}L^{-1}$ BAP were efficient for embryogenic callus induction and regeneration of Miscanthus. This system would be useful for mass-propagation and developing new cultivars via tissue culture of Miscanthus sinensis.

• Current Research on Agriculture and Life Sciences
• /
• v.32 no.1
• /
• pp.10-17
• /
• 2014

This study presents an eco-friendly persimmon drying system to satisfy consumer preferences and provide a popular food for both the domestic and international markets. The most effective antimicrobial compounds were developed from a combination of plant extracts (18.18% clove buds, 9.90% cinnamon, 9.09% licorice, 4.55% cnidium, 4.55% seed of grapefruit, and 54.54% apple vinegar). The dried persimmons were evaluated as regards their moisture and sugar content, weight, hardness, and color value. During the drying process, the overall moisture content of the persimmons increased, along with the sugar content. The hardness was almost the same for each region and decreased on an average of 0.5~0.86 after 6 weeks. As regards the chromaticity, ${\Delta}E$ decreased during the drying process, while L-value became darker and a-value showed a dark red color over time.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.40 no.5
• /
• pp.637-643
• /
• 1995

TR75, a rice (Oryza sativa L. var. Sasanishikj) mutant resistant to 5-methyltryptophan (5MT) was segregated from the progenies of its initial mutant line, TR1. The 5MT resistance of TR75 was inherited in the M$_{8}$ generations as a single dominant nuclear gene, and was also expressed in callus derived from seeds, roots, and anthers as well as in the seedlings. The callus induced from these organs could grow at 50 mg/1 of 5MT, whereas the growth of wild-type callus was completely inhibited even at 25 mg/1. The seedlings of TR75 did not show resistance to L-azetidine-2-carboxylic acid, S-2-aminoethyl-L-cysteine, p-fluoro-DL-phenylalanine. The content of free amino acids in the TR75 homozygous seeds increased approximately 1.5 to 2.0 fold compared to wild-type seeds. Especially, the contents of tryptophan, phenylalanine and aspartic acid were 5.0, 5.3 and 2.7 times higher than those of wild-type seeds, respectively.y.

• Research in Plant Disease
• /
• v.25 no.2
• /
• pp.94-97
• /
• 2019

In February 2019, a damping-off disease occurred at the seedling stage of paprika in a commercial nursery located in Cheorwon, Korea. A species of Fusarium was isolated from the diseased plant and it was identified as Fusarium oxysporum based on morphological characteristics and nucleotide sequence data of translation elongation factor $1-{\alpha}$ and the largest subunit of RNA polymerase. The isolate obtained was revealed to be pathogenic to the host plant through pathogenicity tests, and the reisolation of the pathogen confirmed Koch's postulates. This is the first report of damping-off caused by Fusarium oxysporum on paprika in Korea.

• Korean Journal of Plant Tissue Culture
• /
• v.26 no.4
• /
• pp.281-287
• /
• 1999

Codonopsis lanceolata ("Deoduck" in Korea) is a perennial herb, and belongs to family, Campanulaceae. Its taproot is used a good source of a wild vegetable as well as an herbaceous medicine. In this study, to develop a bialaphos-resistant transgenic Codonopsis, seed germination mechanism and somatic embryogenesis of the plant were investigated, and Agrobacterium-mediated transformation with bar gene encoding phosphinothricin acetyltransferase (PAT) was performed. Attempt were made to regenerate plant from cells via somatic embryogenesis. When the cotyledons, nodes and leaf disks were cultured on MS medium containing 2,4-D and zeatin, embryogenic calli were induced. Upon transferring the somatic embryos to N6 solid medium without plant growth regulators, they developed into plantlets under continuous illumination. All plants were dead on MS basal medium containing 10 mg/L phosphinothricin (PPT) and Basta, respectively. The explants did not produce calli in the medium containing 200 mg/L kanamycin. The explants were cocultured with Agrobacterium tumefaciens for 2 days, and transformants were selected in MS basal medium containing 1.0 mg/L 2,4-D, 100 mg/L kanamycin and 500 mg/L carbenicillin. After the selection, embryogenic calli were induced and then somatic embryos were produced by subsequent subculturing. The somatic embryos were germiated on N6 basal medium containing 200 mg/L kanamycin and 500 mg/L carbenicillin. PCR analysis showed that nptII and bar genes were introduced in the Deoduck transformants. After the confirmation of bar gene expression in RNA and protein level, the transgenic Deoduck will be used to study the genetics of filial generation with the herbicide control gene, bar.gene, bar.

• Korean Journal of Plant Resources
• /
• v.22 no.2
• /
• pp.136-144
• /
• 2009

Germination tests on 3 species that have potential for use as sprout vegetables, such as Coreopsis tinctoria, Saussurea pulchella, and Matricaria recutica, were conducted for 20 days under different temperatures of $15{\sim}30^{\circ}C$, and conditions of light and dark. C. tinctoria showed germination of 83% under $15^{\circ}C$ and dark condition after 4 days, S. pulchella 51.7% under $25^{\circ}C$ and light condition after 20 days, and M. recutica 90.3% under $25^{\circ}C$ and dark condition after 4 days. To investigate optimum plantlet size before greening treatment, seeds germinated were allowed to grow under darkness. The optimum growth of C. tinctoria was obtained under $30^{\circ}C$ after 5 days, S. pulchella under $25^{\circ}C$ after 6 days, and M. recutica under $20^{\circ}C$ after 6 days. Greening treatment resulted in diminished longitudinal growth, but C. tinctoria and S. pulchella showed more vigorous latitudinal growth. Days required before marketing as sprout vegetables were different according to species - Three days of greening was good for C. tinctoria, 2 for S. pulchella, no greening for M. recutica. Generally, tightly sealed containers for minimum water loss were recommended for storage of sprout vegetables after harvest. However, storage methods for each species were different depending on various factors like temperature of storage, presence of ventilation holes of storage packages and forms of marketing. More detailed research for above 3 species is proposed.

• Research in Plant Disease
• /
• v.23 no.2
• /
• pp.202-205
• /
• 2017

Pink rot appeared in greenhouse-grown sunflower (Helianthus annuus L.) in Jeonju, Korea. The symptoms appeared as brown discoloration of sunflower head and progressed into stem in advanced stage. In order to investigate the causal organism of this disease, we isolated a fungus from the infected seeds and maintained the isolated fungal culture on potato dextrose agar medium. Conidiophores were simple or branched, 62.5 to $123.1{\mu}m$ long. Conidia were produced in basipetal chains, ellipsoidal to pyriform with oblique and prominent truncate basal scars, two-celled, hyaline and measured $10.2-21.4{\times}7.5-12.6{\mu}m$. The fungus was inoculated to a new sunflower plant and showed the typical blight on the leaves. Study of morphological characters, pathogenicity tests and sequence analysis revealed that the isolated fungus is confirmed to be Trichothecium roseum. To our knowledge, this is the first report of pink rot on sunflower caused by T. roseum in Korea.

• Korean Journal of Medicinal Crop Science
• /
• v.8 no.4
• /
• pp.334-341
• /
• 2000

This study was conducted to determine the effects and optimum concentrantion of chemical mutagens, colchicine, EMS (ethyl methan sulfonate), MNU (1-methyl-3-1­-nitrosoguanidinenitro), sodium azide $(NaN_3)$ for induction of mutant plants. In order to induce the mutants of Dianthus superbus L, immature seed were pre-soaked in the warter adding each mutagens and concentration of EMS, colchicine, MNU, and sodium azide $(NaN_3)$. Comparision of morphological characteristic and seed germination in each mutant plants differed depending on mutagen sources and their concentrations. When 0.2% EMS were treated on seed, germination decreased to 12% while untreated control was germinated 76.6% for twenty days. Treatments of colchicine appeared higher germination than other mutagen but not survived. The survival rate was extremely decreased in MNU treatment at 0.5mM and chlorophyll-mutant plantlets were obtained by sodium azide treatment at 0.2mM. Chlorophyll mutants were produced by pre-soaking the immature seed of Dianthus superbus L. with mutagen, sodium azide. The control plants appeared normal green leaf color, while mutant plant after mutagenic treatment of immature seed results in yellow­-green stripes and albino in normal green leaf tissue. RAPD was carried out to check the genetic modification of regenerated plants by mutagen treatments at 0.2mM sodium azide. Three polymorphic DNA fragments out of thirty-seven obtained by RAPDs were observed in regenerated plants using five decamer primers.