• The Korea Journal of Herbology
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• v.24 no.4
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• pp.115-120
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• 2009

Objectives : Due to the morphological similarity and frequent occurrence of intermediate forms as well as morphological variations of aerial part, the correct identification between Rhei Radix et Rhizoma and Rhei Undulatai Rhizoma is very difficult. To develop a reliable method for correct identification and improving the quality standards of Rhei Radix et Rhizoma and Rhei Undulatai Rhizoma, we analyzed RAPD and developed SCAR marker. Methods : To amplify target DNA at the genomic level, 32 Operon 10-mer random primers were applied with four Rheum species, R. officinale, R. palmatum, R. tanguticum and R. undulatum. The nucleotide sequences were determined and species-specific primers were prepared depending on the species-specific RAPD amplicons after subcloned into the pGEM-Teasy vector. To develop the SCAR markers, species-specific PCR amplification and multiplex-PCR were carried out using the single species-specific primer pairs and combinations of them, respectively. Results : We used RAPD analysis of four Rheum plant species to obtain several species-specific RAPD amplicons. From nucleotide sequences of these RAPD amplicons, we developed two SCAR markers that amplified 314 bp and 390 bp DNA fragments in only R. undulatum but not in R. officinale, R. palmatum, R. tanguticum and R. undulatum, for distinguishing Rhei Undulatai Rhizoma and Rhei Radix et Rhizoma. Furthermore, we established SCAR markers for the simultaneous discrimination of the three species within a single reaction by using multiplex-PCR. Conclusions : These genetic markers can be used for the efficient discrimination of plants species and commercial herbal medicines between Rhei Undulatai Rhizoma and Rhei Radix et Rhizoma, to ultimately prevent indiscriminate distribution and prescription of these herbal medicines.

• Journal of Environmental Science International
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• v.26 no.8
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• pp.977-991
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• 2017

The purpose of this study is to establish common indicators that constitute a "low-carbon green city" and determine their priorities from the perspective of Incheon Metropolitan City with a view to help develop its climate change strategy strategic city. Several major cities, domestic and overseas, were benchmarked to come up with preliminary indicators consisting of six areas, twenty two planning factors, and 74 indicators. In order to evaluate the validity and relevance of preliminary indicators, expert FGI (Focus Group Interview) was conducted that changed the numbers of final indicators to six areas, twenty two planning factors, and 82 indicators. Finally, AHP (Analytic Hierarchy Process) was conducted to assign relative importance (i.e. weights) to each indicator. Through the layering process of AHP, the upper category of "field" and lower category of "planning factors" were set up as policy prerequisites for constructing a low-carbon green city (6 fields, 22 planning factors). The AHP results for the first level (fields), green city space was ranked first, followed by energy and resource circulation, green traffic, ecological preservation, green logistics, and governance. Among all planning factors, land use, energy efficiency, traffic system improvement, location planning, securing of ecological area, efficiency of logistics, and cooperative organization showed the highest priorities.

• Elastomers and Composites
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• v.34 no.4
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• pp.341-349
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• 1999

In this study, heat and crack resistance of natural rubber (NR) compounds was evaluated. To prevent the effects of the crosslinking system, a conventional vulcanization system was selected, where the accelerator/sulfur ratio was fixed to 0.25. Vulcanizates containing phenylenediamine showed high tensile strength and tear strength compared to other vulcanizates because phenylenediamine can cause additional crosslinking and high dispersion In the vulcanizates. In the pure shear test, vulcanizates containing phenylenediamine showed an excellent tearing energy which was due to the irregular crack path, and showed excellent heat and crack resistance which was also due to the good dispersity of antioxidant and additional crosslinks in the rubbber vulcanizates.

• Journal of Yeungnam Medical Science
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• v.21 no.1
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• pp.74-81
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• 2004

Purpose: To evaluate the computed tomography (CT) findings of pulmonary cryptococcosis in immunocompetent patients. Materials and Methods: CT scans of 25 patients with biopsy-proven cryptococcosis [surgery (n=3), percutaneous needle biopsy (n=21), and bronchoscopic biopsy (n=1)] were analyzed. Thirteen patients were men and 12 patients were women, with a mean age of 53.7 years. Presenting symptoms were cough, sputum, and dyspnea and 12 patients presented with incidentally found chest radiographic abnormalities. Results: Nodule or multiple conglomerate nodules (n=10, 40%) and segmental or lobular consolidation (n=9, 36%) were most common, followed by mixed patterns (n=5, 20%). Predilection sites were lower lobe (n=21/37, 57%) and subpleural areas (n=23, 92%). Air bronchograms within consolidations (n=11/14, 79%) with mild volume loss (n=10/14, 71%) were common. While interlobular septal thickening (n=11, 44%) and cavitation or central low-attenuations (n=11, 44%) were relatively common, lymphadenopathy (n=2, 8%) or free pleural effusions (n=1, 4%) were uncommon. Conclusion: Nodules or airspace consolidation with a predilection of lower lobe and subpleural area are the most common appearances of pulmonary cryptococcosis in immunocompetent patients.

• Korean journal of applied entomology
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• v.43 no.4 s.137
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• pp.329-332
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• 2004

This experiment was conducted to investigate pathogenicity of Spodoptera exigua nucleopolyhedrovirus (SeNPV) with different temperatures for mass production. In laboratory condition, $LC_{50}$ values of SeNPV were $9.797{\times}10^{3}PIBs/mL$ at $20^{\circ}C$ and $3.351{\times}10^{2}PIBs/mL$ at $32^{\circ}C$ in 2nd instar larvae. $LC_{50}$ of the other larval stage were similar to that of 2nd instar. $LT_{50}$ values of SeNPV was 9.0 days in $1.0{\times}10^{3}PIBs/mL$ but 6.9 to 3.5 days in $1.0{\times}10^{4-6}PIBs/mL$ against 3rd instar of Spodoptera exigua. $LT_{50}$ Values of $1.0{\times}10^{4}PIBs/mL$ were 5.7, 5.5 and 4.9 days in 24, 28 and $32^{\circ}C$, respectively. As a results, $LT_{50}$ was shortened with increase of temperatures up to $32^{\circ}C$ and also dependent on viral concentration and larval instars.

• Applied Chemistry for Engineering
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• v.10 no.8
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• pp.1129-1135
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• 1999

The reduction of $SO_2$ by CO over supported cobalt catalysts was investigated within the temperature range of $350{\sim}550^{\circ}C$, initial $SO_2$ concentration of 1000~10000 ppm, $CO/SO_2$ molar ratio of 1.0~3.0 and space velocity of $5000{\sim}15000h^{-1}$. Several types of supports such as ${\gamma}-Al_2O_3$, $TiO_2$ were tested. The $SO_2$ conversion and selectivity to elemental sulfur were investigated using a differential fixed bed reactor at atmospheric pressure. The catalyst prepared by wet impregration of 5 wt % cobalt on ${\gamma}-Al_2O_3$ showed $SO_2$ conversion higher than 90% and COS yield lower than 6% at temperature above $400^{\circ}C$. The optimum $CO/SO_2$ molar ratio was investigated as 2.0. At higher $CO/SO_2$ molar ratio, the $SO_2$ conversion became higher but the main product was COS. The effect of $SO_2$ concentration and space velocity over $SO_2$ conversion and COS yield was not appreciable in the experimental range. The activated cobalt phase was detected as $CoS_2$ and the $CoS_2$ phase unchanged even after reaction.

• Journal of the Korean Wood Science and Technology
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• v.46 no.3
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• pp.278-284
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• 2018

This study is for species identification for each structure member such as Pillar, Bo, Changbang, Dori, Jangyeo, Judu, Donjaju, Chunyeo, Guitle, and Jongdae, of Pungnammun Gate (Treasure 308). Jeonju is the birthplace of Joseon Dynasty and Pungnammun Gate was the southern gate of old Jeonjueupseong which was walled town. Provincial Governor of Koryeo Dynasty, Yu Gyeong Choi built Jeonjubuseong and four gates at all cardinal points in 1388. And the gate was burnt down by Jeongyujaeran (war with Japan in 1597). It was rebuilt by King Yeongjo (Joseon Dynasty) in 1734 and renamed 'Pungnammun' after 34 years. It was designated for Treasure 308 for its unique style of architecture and historic values in 1963. In this study, all of wooden structure members were Pinus spp.. This result was matched for the result of major species for wooden building of late Joseon Dynasty. It can be used to complete database for architecture of Castle's Gate and help for restoration of cultural heritage in the future.