• The Korean Journal of Zoology
• /
• v.34 no.4
• /
• pp.571-584
• /
• 1991

잉어과(Gyprinidae)의 Zocco속 어류 4종과 Candidia속 어류 1종에 대한 종간 유연관계와 종분화 연대측정 및 이들의 지질학적 분포경로를 밝히기 위하여 한국, 일본 및 대만에서 채집된 개체를 대상으로 전기영동법에 의한 유전자 분석을 하였다. 각 종의 지역별(한국, 일본, 대만) 집단간 유전적 유연관계를 분석한 결과 평균 유전적 근연치는 90% 이상이었다. Z. temmincki의 경우 일본집 단들은 한국의 A-type 집단과는 유연관계가 가까왔으나 한국의 B-type과는 유전적 차이가 현저하러다. Z. PlaD여5의 경우 한국집단과 일본집단사이의 유연관계는 S = 0.852였고, 한국집단과 대만집단 사이는 5 = 0.672, 일본집단과 대만집단 사이는 S = 0.751로서 지리적으로 현저한 차이를 업였다. Z. pochycephalus 3개 집단간의 유전적 근인치는 S = 0.963이었고 Candidia borbota 2개 집단간은 S = 0.946이었다. 종간의 유전적 근연환계를 비교한 결과 Candidia borbota와 Z. temmincki사이는 S = 0.608, Z. pluDpus와 1. pachycephalus사이는 S = 0.612였으나, Z. temmincki와 Z. platypus사이는 S = 0.441, Z. temmincki 와 Z. pochycepholus 사이는 S = 0.350이었고, Z. plotpus와 Condfda barbata사이는 S = 0.328로서 이들 사이에는 현저한 유전적 차이가 있었다. 각 종간의 롱분화 연대를 추산한 결과 이들은 약 480만년 전인 Pliocene 초기에 공통 조상종에서 분화하여 Z. temmincki, Candidia borbato group과 Z. plotypus, Z. pochycepholus group으로 분리되었고 약 260만년 전인 Pliocene 후기에 Z. temminc소와 Candidia borbota로 분화되었다고 추산되며 약 80만년 전인 Pleistocene시기에 남 temmincki B-type에서 h-type이 분화되었다짙 여겨진다. 한편 또 다른 한 단opP은 약 230만년 전인 열iocene후기에 대만 지역의 Z. plotypas에서 Z. pochvcepholus가 분화된 후 현재에 이르렀다고 추정된다. Z. platypus는 약150만년 이전인 초기 Pleistocene시기에 대만지역에서 한국 및 일본집단으로 분리되었다고 보며 이들 한국집단과 일본집단은 약 50만년 전 Pleistocene의 Middle기에 고황하 수계를 거쳐 현재의 분포 상황에 이르렀다고 여겨진다. 한편 대. temmin체과의 B-type에서 저온 적응으로 분화되었다고 추측되는 A-type은 약 20만년 전인 Pleistocene의 Riss기에 역시 고황하 수계를 통하여 한국과 일본으로 분포하여 현재에 이르렀다고 사료된다.

• The Korean Journal of Mycology
• /
• v.12 no.4
• /
• pp.164-169
• /
• 1984

Interspecific heterokaryons were obtained between auxotrophic mutants of Pleurotus ostreatus and Pleurotus florida by polyethylene $glycol-Ca^{++}$ induced fusion of somatic protoplasts. The fusion products produced colonies of dense growing mycelium after 10-14 days culture on hypertonic Mushroom Minimal Medium. When they were transferred to Mushroom Minimal Medium plates, the sectors showed normal vegetative morphology but the colonies of irregular shape varied in growth rate. All of the colonies produced fruit body of normal pilei. Some heterokaryon colonies bearing none or only a small amounts of basidiospores were isolated. Fusion products, generally, gave higher basidiocarp yields than the parents.

• FLOWER RESEARCH JOURNAL
• /
• v.16 no.4
• /
• pp.321-324
• /
• 2008

A new yellow graft cactus Gymnocalycium mihanovichii ${\times}$ marsoneri cv. 'Hangseong' was developed by the National Institute of Horticultural &Herbal Science, Rural Development Administration in 2005. 'Hangseong' was selected from a interspecific crossing between Gymnocalycium mihanovichii and G. marsoneri. In 1998, 2 lines of '9834024' and '9834036' were selected from a crossing between G. mihanovichii 'IG-124' and G. marsoneri 'IG-223'. The 2 lines were crossed between them. In 2001, progenies produced from a crossing of the 2 lines were nursed and, finally yellow colored 'Wonkyo G1-169' was selected through 3 times of characteristic trials. It was named as 'Hangseong' and applied for a variety protection. Characteristics of 'Hangseong' is as follows: The color of body and tubercles is yellow. The shape of glove is flat with 7 to 9 deep ribs. Spine is medium straight with long size and brown color. Growth is fast as to reach 49.5 mm in diameter at 10 month after planting. Propagation ability is good as to set as many as 7.2 tubercles. 'Hangseong' is suitable for export to European countries or America. Characteristics of the cultivar can be maintained by vegetative propagation.

• Food Science of Animal Resources
• /
• v.28 no.3
• /
• pp.276-282
• /
• 2008

It is estimated that over 80% of deer antlers produced in the world are consumed in Korea. However, mislabeling or fraudulent replacement of costly antlers with cheaper ones is one of the most common problems in the domestic antler market. Therefore, there is a great need for the development of technology to identify species of antlers. This study was carried out to develop an accurate and reliable method for the identification and authentication of species or subspecies of antlers using DNA sequence analysis and comparison of mitochondrial cytochrome band D-loop region genes among antlers of five deer species, Cervus elaphus sibericus, Cervus elaphus canadensis, Cervus nippon, Cervus elaphus bactrianus and Rangifer tarandus. A variable region of cytochrome band D-loop genes was amplified using PCR with specifically designed primers and sequenced directly. The cytochrome band D-loop region genes showed different DNA sequences between the species of antlers and thus it is possible to differentiate between species on the basis of sequence variation. To distinguish between reindeer (Rangifer tarandus) antlers and other deer antlers, PCR amplicons of the cytochrome b gene were digested with the restriction enzymes NlaIV and TaqI, respectively, which generates a species-specific DNA profile of the reindeer. In addition, samples of 32 sliced antlers labeled Cervus elaphus sibericus from commercial markets were collected randomly and the mt DNA D-loop region of these antler samples was sequenced. Among the antler samples investigated, only 62.5% were from Cervus elaphus sibericus, and others were from Cervus elaphus bactrianus (25.0%), elk (Cervus elaphus canadensis) and reindeer (Rangifer tarandus). Our results suggest that DNA sequencing of mt DNA and PCR-RFLP methods using NlaIV and TaqI enzymes are useful for the identification and discrimination of deer antler species by routine analysis.

• Journal of Korean Society of Forest Science
• /
• v.29 no.1
• /
• pp.102-114
• /
• 1976

This experiment was conducted to investigate the percentage of fertile seed in terms of crossabilities and relationships of taxonomic affinities for the ${\times}$ P. rigitaeda of interspecific hybrid, ${\times}$ P. rigida rigitaeda and ${\times}$ P. rigitaeda rigida of backcross hybrids, $F_2$ of ${\times}$ P. rigitaeda and natural hybrid of ${\times}$ P. rigitaeda within Sub-genus Diploxylon of the Genus Pinus. The possibility of establishment of hybrid seed orchard and differentia of hybrids for the purpose of extensive program of reforestation in the future have also been investigated. And, the experimental results obtained are summarized as follows: 1. On the basis of crossabilities as well as on the taxonomic affinities according to the systems of Shaw, Pilger and Duffield, it has been proven that the parental species of those hybrids are of close affinities and range of the fertile hybrid seed production rate was as high as 67-87% in the best hybrid combination (Table 6). 2. Those hybrids seemed to be most promising in the growth perfermance exhibiting 28-80% more volume growth compared to the P. rigida with the statistic significance of 1-5% level (Table 7, 8, 9). And all hybrids exhibit cold hardiness as much as P. rigida except $F_1$ hybrid of ${\times}$ P. rigitaeda and it seems to suggest that the characteristics of cold hardiness were transmitted from the P. rigida. 3. With regard to the anatomical characteristics of needle, the hypoderm is biform in most of the hybrid pines and the characteristics of resin canals are medial in all hybrid. And, the fibrovascular bundles are intermediate of both parent in all hybrid. Therefore it was found to be possible to distinguish the hybrids pines from their parents by the needle characteristics (Table 10). 4. It has been demonstrated that the hybrids pines have a phenolic substance (No. 7) of pale yellow at Rf-0.66, same as P. rigida, but no trace of phenolic substance was observed in the P. taeda. This fact will serve as an important criteria for early identification of hybridity in progeny testing (Table 11). 5. It was found to be possible to distinguish by the starch gel electrophoretic variations banding patterns and staining densities of isoperoxidase in the needles of the hybrids pines from their parents (Fig. 1).

• Korean Journal of Weed Science
• /
• v.9 no.2
• /
• pp.168-173
• /
• 1989

In the characteristics of intra-specific competition according to bulrush density, the intra-specific competition in plant height began at 5 and 15% per pot in mixed culture and bulrush monoculture, respectively. The intra-specific competition in the shoot began at 5 plants per pot in both mixed culture and bulrush monoculture. The competition in dry matter weight began at 15 plants per pot in monoculture, and at 10 plants in mixed culture. On the rice density, the plant height, and numbers of shoot and dry matter weight in rice were rapidly increased up to 2.6, and 2.5 plants per pot, respectively, And then the increments were slow, The critical intra-specific competition of bulrush with rice was 2.5 to 3 plants per pot in their traits of rice, and especially the number of shoots and dry matter weights per pot showed significant decrements. In the different transplanting time in rice, the plant heights in both rice and bulrush elongated longer in mixed culture than in monoculture. The plant height of bulrush became shorter but the height of rice longer with late transplanting. The ratio of shoot number in rice to the number in bulrush was about 1 : 3 in all plots, and shoot numbers became less by late transplanting. The dry matter weight in both species were decreased by late transplanting, and the decrements were more prominent in bulrush than in rice.

• Proceedings of the Korean Society for Agricultural Machinery Conference
• /
• 2017.04a
• /
• pp.162-162
• /
• 2017

구글에서 공개한 Tensorflow를 이용한 여러 학문 분야의 연구가 활발하다. 농업 시설환경을 대상으로 한 빅데이터의 축적이 증가함과 아울러 실효적인 정보 획득을 위한 각종 데이터 분석 및 마이닝 기법에 대한 연구 또한 활발한 상황이다. 한편, 타 분야의 성공적인 심층학습기법 응용사례에 비하여 농업 분야에서의 응용은 초기 성장 단계라 할 수 있다. 이는 농업 현장에서 취득한 정보의 난해성 및 완성도 높은 생육/환경 모델링 정보의 부재로 실효적인 전과정 처리 기술 도출에 소요되는 시간, 비용, 연구 환경이 상대적으로 부족하기 때문일 것이다. 특히, 센서 기반 데이터 취득 기술 증가에 따라 비약적으로 방대해진 수집 데이터를 시간 복잡도가 높은 심층 학습 모델링 연산에 기계적으로 단순 적용할 경우 시간 효율적인 측면에서 성공적인 결과 도출에 애로가 있을 것이다. 매우 높은 시간 복잡도를 해결하기 위하여 제시된 하드웨어 가속 기능의 경우 일부 개발환경에 국한이 되어 있다. 일례로, 구글의 Tensorflow는 오픈소스 기반 병렬 클러스터링 기술인 MPICH를 지원하는 알고리즘을 공개하지 않고 있다. 따라서, 본 연구에서는 심층학습 기법 연구에 있어서, 예상 가능한 다양한 자원을 활용하여 최대한 연산의 결과를 빨리 도출할 수 있는 하드웨어적인 접근 방법을 모색하였다. 호스트에서 수행하는 일방적인 학습 알고리즘과 달리 이기종간 심층 학습이 가능하기 위해선 우선, NFS(Network File System)를 이용하여 데이터 계층이 상호 연결이 되어야 한다. 이를 위해서 고속 네트워크를 기반으로 한 NFS의 이용이 필수적이다. 둘째로 제한된 자원의 한계를 극복하기 위한 메모 공유 라이브러리가 필요하다. 셋째로 이기종간 프로세서에 최적화된 병렬 처리용 컴파일러를 이용해야 한다. 가장 중요한 부분은 이기종간의 처리 능력에 따른 작업을 고르게 분배할 수 있는 작업 스케쥴링이 수행되어야 하며, 이는 처리하고자 하는 데이터의 형태에 따라 매우 가변적이므로 해당 데이터 도메인에 대한 엄밀한 사전 벤치마킹이 수행되어야 한다. 이러한 요구조건을 대부분 충족하는 Open-CL ver1.2(https://www.khronos.org/opencl/)를 이용하였다. 최신의 Open-CL 버전은 2.2이나 본 연구를 위하여 준비한 4가지 이기종 시스템에서 모두 공통적으로 지원하는 버전은 1.2이다. 실험적으로 선정된 4가지 이기종 시스템은 1) Windows 10 Pro, 2) Linux-Ubuntu 16.04.4 LTS-x86_64, 3) MAC OS X 10.11 4) Linux-Ubuntu 16.04.4 LTS-ARM Cortext-A15 이다. 비교 분석을 위하여 NVIDIA 사에서 제공하는 Pascal Titan X 2식을 SLI로 구성한 시스템을 준비하였다. 개별 시스템에서 별도로 컴파일 된 바이너리의 이름을 통일하고, 개별 시스템의 코어수를 동일하게 균등 배분하여 100 Hz의 데이터로 입력이 되는 온도 정보와 조도 정보를 입력으로 하고 이를 습도정보에 Linear Gradient Descent Optimizer를 이용하여 Epoch 10,000회의 학습을 수행하였다. 4종의 이기종에서 총 32개의 코어를 이용한 학습에서 17초 내외로 연산 수행을 마쳤으나, 비교 시스템에서는 11초 내외로 연산을 마치는 결과가 나왔다. 기보유 하드웨어의 적절한 활용이 가능한 심층학습 기법에 대한 연구를 지속할 것이다

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.7 no.1
• /
• pp.145-151
• /
• 1969

To make clear the species of Korean Asiatic cotton, 21 Asiatic cotton varieties collected from China, Manchuria, Japan and Korea and preserved at suwon Crop Experiment Station were crossed to the tester stocks and cytological studies were made for their $F_1$ pollen mother cells. The results were summarized as follows: 1. In the all $F_1$ hybrids between the 21 collections and G. herbaceum tester stocks always one ring-four association was observed. 2. In the $F_1$ hybrids between additional 5 collections and G. arboreum tester stock ring-four or chain-four was not observed. 3. In the $F_1$ hybrids between G. herbaceum tester stocks and between G. arboreum tester stocks no ring-four or chain-four was observed, while in the $F_1$ hybrids between G. herbaceum tester stock and G. arboreum tester stock always one ring-four was observed. In the $F_1$ hybrids between collections also no ring-four or chain-four was observed. 4. From above results and together with the results reported in previous paper the species of Asiatic collections was inferred to the D.U. Gerstel's G. arboreum and their race was inferred to the J. Hutcinson's G. arboreum L. race sinense.

• Journal of Ginseng Research
• /
• v.28 no.4
• /
• pp.191-195
• /
• 2004

An investigation was conducted to ascertain the basic information on characteristics of embryonic tissue development among Korean Ginseng, American ginseng, $F_{1}$ hybrids and $BC_{1}F_{1}(F_{1}$ pollen back cross to $BC_{1}=Korean\;Ginseng)seeds$. The specific activities of lipoxygenase (LOX) and the protein assays were made during the above embry­onic growth period at 5 or 25 degree C. The results are summarized as follows: The fresh weights(mg) of germinating seeds were significantly different among species. Ginseng seeds were stratified at $5^{\circ}C\;for\;60\;days$ the ratios of embryo/endosperms in $P.g.,\;P.q\;or\;F_{1}\;were\;about\;90{\%}$. The ratio was in $BC_{1}F_{1},\;79.2{\%}$ during the same period. The ratios of embryo/endosperms of seeds of P.g. or P.q. germinated at $5^{\circ}C$ showed rather higher values as compared to that at $25^{\circ}C$ LOX specific activity the highest in the $F_{1}$ and decreased in the order of P.g., P.q., and $BC_{1}F_{1}$. The highest LOX specific activity was shown at 80 days after sowing, followed by 70 days sowing, the least LOX activity was shown at 60 days.

• The Korean Journal of Mycology
• /
• v.17 no.3
• /
• pp.137-144
• /
• 1989

Effects of incompatibility existing between intra-and interspecies in Pleurotus spp. on protoplast fusion, clamp formation of their fusants and fruitbody production were investigated. Protoplast fusion between intra-and interspecies of the fungus was achieved by Poly ethylene glycol treatment. The fusion frequency between intraspecies was a little higher than that of interspecies. Fusion frequency between interspecies was not correlated with their similarities based on isozyme patterns. In case of protoplast fusion between intra-and interspecies, the fusants from the compatible isolates produced normal fruit bodies, while those from the incompatible isolates did not produce clamp connections and fruit bodies except those of a few isolates presumed mutants.