• Korean Journal of Plant Taxonomy
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• v.33 no.4
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• pp.421-433
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• 2003

To examine the leaf epidermal microstructure, nine species in five genera (Daphne L. - 4 spp., Diarthron Turcz. - 1 sp., Edgewarthia Meisn. - 1 sp., Stellera L. - 1 sp., Wikstroemia Endl. - 2 spp.) of the Korean Thymelaeaceae were investigated by light microscopy (LM) and scanning electron microscopy (SEM). The stomata of stuo야ed taxa were 'hypostomatic type' and the size range of guard cell was $13.8-34.4{\times}8.7-22.9{\mu}m$: the smallest size of stomata was found in Diathran linifolium ($15.9{\pm}2.6{\times}10.0{\pm}1.3{\mu}m$), while the largest one was measured to Daphne adara ($32.8{\pm}1.6{\times}20.7{\pm}1.3{\mu}m$). The stomatal complex was anomocytic in the most studied taxa, except Daphne kiusiana by having combined with anisocytic together. The shapes of epidermal cells are undulate anticlinal wall. The size range of epidermal cell was $20.7-61.0{\mu}m$; the smallest size of epidermal cell was found in Stellera charnaejasme ($26.0{\pm}1.9{\mu}m$), on the other hand the largest one was found in Edgeworthia chrysantha ($53.6{\pm}3.1{\mu}m$). The well-developed flaky epicuticular waxes can be divided three kinds of pattern - (1) smooth in comparison, not entire platelets and scattered, (2) isolated flake-like platelets, mostly paralleled, sparsely, (3) flake-like platelets, flat, membraneous, protruding from the surfaces at varying angles and densely. Two types of trichome are recognized; (1) Type I: uniseriate trichome of striate surface (D. genkwa, Diarthron linifalium, E. chrysantha, W. ganpi and W. trichotama), (2) Type II: multicellular trichome of papillose surface, uncinated 3-4 nodes (Diathron linifolium). Finally, the systematics significance of the leaf micromorphological features in identification and elucidation of Korean Thymelaeaceae, especially between or within the genera including among the species is also briefly discussed.

• Korean Journal of Environment and Ecology
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• v.23 no.5
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• pp.418-430
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• 2009

The main purpose of this study is to investigate the relationship between the topography of land and vegetation and the preferred habitat of wintering cranes. Investigations were conducted twelve times in Cheorwon basin, South Korea, during two wintering seasons (12/2005 - 2/2006; 1/2007 - 2/2007). The density of rice grain in the middle of rice paddies was higher than that of the areas along the edge of rice paddies. However it was observed that red-naped cranes preferred to feed along the edges of rice paddies rather than to feed in the middle of the paddies. White-naped cranes, on the other hand, Preferred to feed in the middle of paddies. To be more specific, red-crowned cranes preferred feeding sites such as levees of the paddies or the areas where the level of the rice beds was comparatively more elevated. But the preference of the white-naped cranes turned out to be just the opposite. Another finding was that both red-naped cranes and white-naped cranes preferred concealed areas for their feeding site, and the frequency rate of their feeding in concealed areas has little to do with weather factors. This finding contradicts a widely accepted view that cranes prefer open spaces for their feeding site. Besides, red-crowned cranes, compared with white-naped cranes, preferred to feed in more concealed areas. The frequency rate of feeding in both concealed areas and non-concealed areas had little to do with the size of feeding flocks. There was no difference between a flock of fewer than five cranes and a flock of more than five cranes in terms of frequency rate of their feeding. In conclusion, the result of these investigations indicate that red-naped cranes comparatively prefer concealed areas for their feeding site, and white-naped cranes are less prone to them, and there is no direct connection between their preference of feeding site or frequency and the size of their flock. This is presumed to be the characteristics unique to their individual species.

• Journal of Bio-Environment Control
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• v.18 no.2
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• pp.153-159
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• 2009

This study was carried out to investigate effect of $GA_{3}$ foliar application on the growth and flowering of Limonium spp. 'Ocean Blue' to develop an effective $GA_{3}$ treatment method for a year round commercial production. Plants were treated with different concentrations of $GA_{3}$ 0, 100, 200, 400, and 800 mg.$L^{-1}$ in summer, and 0, 100,200, and 400 mg.$L^{-1}$ in winter at seven weeks after transplanting. The effects of $GA_{3}$ treatment time (0, 10, 20, 30, 40, and 50 days after planting) and plant age (raising during 3, 5, 7, and 9 weeks) was investigated. The $GA_{3}$ treatment advanced bolting and flowering with little difference between the two seasons. In a summer culture the effective concentration of $GA_{3}$ ranged from 200 to 400 mg. $L^{-1}$, while in winter it was found to be 400 mg. $L^{-1}$. The $GA_{3}$ treatment at 10 days after planting significantly reduced days to flowering. However, the greatest cut flower yield was observed in plants treated at 20 days after planting. The $GA_{3}$ treatment to different age plants did not show a significant effect, except in the oldest (nine weeks old plants). In conclusion, to induce early flowering in Limonium spp. 'Ocean Blue', the proper concentration and time of application of $GA_{3}$ was 400 mg.$L^{-1}$ $GA_{3}$ treated to nine weeks old plants at 20 days after transplanting.

• The Korean Journal of Zoology
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• v.23 no.2
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• pp.89-108
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• 1980

Comparative studies on the parafollicular cells of the some mammalian species from five different orders were carried out; i.e., man from Primates, cattle, pig, and black goat from Artiodactyla, dog from Carnivora, rabbit from Lagomorpha, rat, mouse, and squirrel from Rodentia. For this study, various special techniques for the parafollicular cells, including Grimelius' silver impregnation method (Sawicki and Bajko, 1974), Singh's argentaffin method (Singh, 1964), HCl-toluidine blue stain (Sawicki, 1971), and HCl-lead hematoxylin stain (Solcia et al., 1969), were applied. Authors obtained the following results: 1. Number of parafollicular cells in the same area of thyroid tissues are significantly different from species to species. Number of cells were largest in dog and less cells were found in the following orders; rat, squirrel, mouse, rabbit, cattle, pig, black goat and finally the smallest number in man. 2. Distribution of parafollicular cells within thyroid gland are significantly different from portion to portion in case of cattle, rabbit, squirrel and mouse, but it is not significant in dog, man, pig, black goat and rat (see Table 1-1 and 1-2). 3. In dog, clustered parafollicular cells are located usually in the interfollicular space, and groups of parafollicular cells are located in the para-and/or inter-follicular positions in rabbit. But in the other animals parafollicular cells are found solitarily in the intra-and/or para-follicular positions. 4. The shape of parafollicular cells shows oval to round contour in dog, but it is polymorphic, for example, spindle, conical, oval, round or elongated with cytoplasmic processes, in the other animals. 5. Size of parafollicular cells is larger in cattle, dog and pig, smaller in rat, mouse and squirrel, and medium-size in rabbit, man and black goat. 6. Parafollicular cells of pig, cattle, dog and squirrel are observed to contain densely packed granules, whereas those of mouse, rat and man contain relatively scanty granules. 7. Parafollicular cells of all the mammals show more or less positive reaction to Grimelius' argyrophile silver impregnation method, HCl-toluidine blue stain and HCl-lead hematoxylin stain, whereas they show negative reaction to argentaffin method (see Table 2). 8. Considering the above finding, it is concluded that there are species differences in the distribution, location and shape of parafollicular cells, and infer that preferable staining method should be selected for reliable detection of parafollicular cells, beacuse staining methods applied on the cells in this study show variable reactions according to species.

• Journal of Gastric Cancer
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• v.8 no.4
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• pp.182-188
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• 2008

Purpose: Transcriptional factors of CREB (cAMP response element binding protein) are involved in regulating the gene expression in response to a variety of signaling pathways. The proteins produced by the CREB genes play key roles in many physiological processes, including memory and long-term potentiation. The retinoic acid receptor (RAR) axis mediates epithelial cell differentiation and proliferation in many tissues. This study examined the expressions of RAR and CREB and their relationship with the clinicopathologic factors and their significance. Materials and Methods: The levels of the RAR and CREB expressions were measured in 150 gastric adenocarcinomas by performing immunohistochemical staining. Results: 1. An RAR protein expression was found in 63.3% of the adenocarcinomas (95/150) and a CREB expression was found in 60.7% (91/150) of the adenocarcinomas. 2. An RAR protein expression was found in 72.2% (78/108) of the intestinal type adenocarcinomas and in 40.5% (17/42) of the diffuse type adenocarcinomas (P<0.05). Based on the depth of invasion, an RAR protein expression was found in 58.3% (14/24) of the T1 adenocarcinomas, in 61.9% (13/21) of the T2 adenocarcinomas, in 63.5% (61/96) of the T3 adenocarcinomas, in 77.8% (7/9) of the T4 adenocarcinomas and in 74.7% (62/83) of the adenocarcinomas with lymph node metastasis and in 49.2% (33/67) of the adenocarcinomas without lymph node metastasis (P<0.01). 3. A CREB expression was found in 69.4% (75/108) of the intestinal type and in 38.1% (16/42) of the diffuse type (P>0.05). Based on the depth of invasion, a CREB expression was found in 50% (12/24) of the T1 adenocarcinomas, in 52.4% (11/21) of the T2 adenocarcinomas, in 64.6% (62/96) of the T3 adenocarcinomas, in 66.6% (6/9) of the T4 adenocarcinomas, in 71.1% (59/83) of the adenocarcinomas with lymph node metastasis and in 47.8% (32/67) of the adenocarcinomas without lymph node metastasis (P<0.01). 4. The RAR protein and CREB expressions coincided in 71.4% of the gastric adenocarcinomas and a significant correlation between them was found (P<0.05). Conclusion: We found a significant relationship between the expression of RAR and CREB and the histology and lymph node metastasis of gastric cancer. Further studies are needed to confirm their biologic meaning in gastric carcinogenesis.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.34 no.2
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• pp.192-197
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• 1989

Two sweet corn hybrids, 'Tanok l' and 'Golden Cross Bantam 70 (GCB 70)' were grown at five plant densities, of 4, 167. 5,556, 6,667. 8, 333, and 11, 111 plants per 10 ares, with or without tiller removal, to determine effects of tiller removal on growth and yield of sweet corn hybrids at various plant densities. Tillers were pulled when less than 15 cm tall. The number of tillers per plant linearly decreased as plant density increased. The two hybrids had similar plant height, ear length and diameter, ear weight and the number of ears per plant and 10 ares. Tanok 1 lodged approximately 20％ at above 8,333 plants per 10 ares, while GCB 70 did not lodge at all, at any plant density. Tanok 1 had higher leaf area index (LAI), ear and stover yields than GCB 70. Except for root lodging and LAI, hybrid x plant density interaction was not significant at 5％ probability level. Plant density did not affect silking data. Increasing plant density linearly increased plant height, LAI, and stover yield, but linearly decreased ear length, ear weight, and the number of ears per plant. Increase in LAI was greater in Tanok 1 than in GCB 70, with increasing plant density. The relationships between the number of ears and ear yield per 10 ares and plant density were Quadratic. The optimum plant density was estimated to be approximately 6500 plants per 10 ares, using the equation based on ear yield. Except for ear height and LA I, hybrid x tiller removal and plant density x tiller removal interactions were not significant. Hybrid x plant density x tiller removal interaction was not significant for any characters. When averaged over hybrids and plant densities, tiller removal reduced plant height and ear and stover yields by about 3, 10, and 16％, respectively, but did not significantly affect silking date, root lodging, ear length and diameter and the number of ears per plant and per 10 ares. The results indicate that the optimum plant density is approximately 6500 plants per 10 ares, regardless of tiller removal and tillers are not to be removed at any plant density.

• Journal of Animal Science and Technology
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• v.46 no.2
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• pp.227-234
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• 2004

This experiment was conducted at Cheonan Yonam Experimental Livestock Farm in 2001-2002 to detennine the effect of harvest date(20 April, 26 April and 4 May) on forage yield and quality of rye varieties of three different maturing groups(early maturing variety = ‘Koolgrazer’ midseason maturing variety = ‘Horni122’ and late maturing variety = ‘Danko’). The heading date of Kooigrazer, Hornil22 and Danko were 17, 22 and 29 April, respectively. Dry matter percentage increased from 15.6 to 21.6% as the harvest date was delayed from April 20 to May 4. Among the rye varieties tested, dry matter percentage of Koolgrazer, Hornil22 and Danko were 20.9, 18.8 and 16.3%, respectively. Dry matter yield increased from 1l.2 to 13.9 ton/ha as the harvest date was delayed, but no significant difference among rye varieties. As the harvest date was delayed, total digestible nutrients(TDN) yield also increased significantly from 7.4 to 8.4 ton/ha However no significant difference was found among rye varieties. Crude protein(CP) percentage decreased from 20.3 to 17.1% as the harvest date was delayed, and CP percentage of late maturing variety, Danko, was significantly higher than that of the other varieties. In content of fiber component(NDF, ADF, ADL, hemicellulose and cellulose) of rye, the late harvest date(4 May) showed the highest among harvest dates. From comparisoo within rye varieties tested, Kooigrazer, a early maturing variety had higher than a rnidseason and late maturing varieties, Hornill22 and Danko. The cellulase digestible of organic matter of dry matter(CDOMD) decreased as the harvest date was delayed. Among the rye varieties tested, the CDOMD of a early maturing variety, Koolgrazer was the lowest. Our study differences of winter rye in forage yield and quality resulting from variety maturity and harvest stage. A early maturing variety, Kooigrazer should be harvested between 24 and 28 April, Hamill 22(midseason maturing variety) harvested between 29 April and 3 May, Danko(late maturing variety) harvested between 4 and 8 May for maximum forage yield and optimum quality.

• Korean Journal of Agricultural and Forest Meteorology
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• v.13 no.3
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• pp.123-139
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• 2011

It is expected that identification and lists of $C_4$ plants in specific regions are useful not only for the ecological researches that are related to vegetation phenology and succession but also as an index of climate change. In this review, $C_4$ plants growing in South Korea were listed and their life forms were investigated. In addition, we discussed the influences that climatic change and the $C_4$ plants exerted on plant ecosystem. Photosynthetic pathway types ($C_3$ and $C_4$) for the plant species in South Korea were determined by reviewing the scientific literatures published between 1971 and 2010. Of the total 4476 species in 1123 genera and 197 families, 206 species (4.6%) in 84 genera (7.5%) and 21 families (10.7%) were identified as $C_4$ plants (including $C_3$-$C_4$ intermediate plants). Among the identified $C_4$ species, 53 species (25.7%) in 26 genera and 15 families were classified as Dicotyledoneae, while 153 species (74.3%) in 58 genera and 6 families were classified as Monocotyledoneae. The majority of the $C_4$ species belong to four families: Chenopodiaceae (15 species), Amaranthaceae (13 species), Gramineae (102 speceis) and Cyperaceae (45 species). With respect to life form composition of 206 $C_4$ species, Th-$R_5$-$D_4$-t was most dominant: 95 species (46.1%) were included in Th, 123 species (59.7%) in $R_5$, 179 species (86.9%) in $D_4$, and 122 species (59.2%) in t. The projected increase in temperature due to climate change may provide better conditions for the growth of $C_4$ plants. Such a result will have considerable impacts on the interspecific competition between $C_3$ and $C_4$ plants, the distribution of $C_4$ plants, plant phenology, and plant diversity.

• Journal of Sericultural and Entomological Science
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• v.14 no.2
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• pp.81-92
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• 1972

Studies on Pathogenicity of Nosema bombycis Naegeli are summarized as follows: 1. The mortality of the parents, Jam 103 and Jam 104, is remarkbly higher than that of the hybrid, Jam 103$\times$Jam 104, whereas there is no difference in the mortality between the parents. 3. In the mortality of the pathogen-concentration, it is increased in order of the following concentrations inoculated, 10$^{8}$ , 10$^{7}$ , 10$^{8}$ and 10$^{5}$ /ml. 3. In the mortality of each instar, it is high in order of 5th, 4th, 3rd, and 2nd instar. 4. In the interaction between the mortalities of the varieties and the concentrations, 1) The mortality shows no differences between the parents and the hybrid in the high concentration of 10$^{8}$ /ml. 2) The mortality of the hybrid is lower than that of the parents in the low concentration of 10$^{5}$ /ml, whereas no difference is found between the parents. 3) The interaction appears at the same level in the middle concentration of 10$^{6}$ /ml to the parents and of 10$^{7}$ /ml to the hybrid. 5. It was pointed out that active immunity depends upon the volume of antigen injection, immunizing period, and injection intervals. In this experiment, it is noticed that the optimum volume of injection is above 20ml and D is the best one of the four treatment (A.B.C.D.). 6. The immune sera indicate such a 12,800 high titer in the indirect method can be obtained from the D immunizing method. Silkworm tissues and N. bombycis spores show self-fluorescence, but it is able to distinguish it from the F.I.T.C. by using the U.V. filter. 7. The midgut epithelium is examined to be the first site of the tissues which are penetrated into and multiplied by the inoculation of the pathogen per os.

• Korean Journal of Weed Science
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• v.7 no.2
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• pp.144-155
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• 1987

Water extracts of polygonum hydropiper and Polygonum aviculare completely inhibited the germination of lettuce seeds. Methanol extracts from these two species also inhibited the seed germination of lettuce (Lactuca sativa) and Oenothera odorata. Fifteen phenolic acids in total were identified by GLC from P. hydropiper and eighteen from P. aviculare. The most common phenolic acids identified from P. hydropiper were sinapic, salicylic+vanillic and ferulic acid presented in all the fractions. In addition, salicylic+vanillic, tannic+gallic, sinapic, ferulic and p-coumaric acid seemed to be important phenolic compounds in terms of quantity. However, salicylic+vanillic acids were the unique phenolic acids occurred in all the fractions of P. aviculare. The others such as tannic+gallic, sinapic, ferulic, p-coumaric acid, p-cresol and catechol present in large amount appeared also the important phenolic substances influencing allelopathic effects of P. aviculare. Linolenic acid and oxalic acid were the major fatty and organic acids in both plant species, presented in 2.38mg/g and 20.588mg/g in P. hydropiper, 3.70mg/g and 14.288mg/g in P. aviculare, respectively, which seem to be exhibiting allelopathic effects of these plants. Total alkaloids were presented in low amount such as 0.20% in P. hydropiper arid 0.22% in P. aviculare which may not be important elements. Pet. ether extracts were 2.42% in P. hydropiper and 1.65% in P. aviculare, which exhibit another potential for allelopathic effects that need further investigation. Various authentic phenolic compounds at different concentrations inhibited the germination of lettuce seed, indicating that the phenolic substances identified here may be directly related to biologically active substance.