• Journal of Practical Agriculture & Fisheries Research
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• v.12 no.1
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• pp.47-52
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• 2010

Squash cultivars of Cucurbita moschata have good fresh quality, but their viny growth habit hinders wide cultivation under structure. On the other hand, 'Zucchini' varieties of C. pepo have bush growth habit and better growth ability under the low temperature condition than C. moschata, but have inferior flesh quality. To combine desirable characteristics of the two species, many attempts have been exerted to obtain interspecific hybrids. A shootless phenomenon was found among segregating populations; the cotyledon expanded normally but further shoot growth. The inheritance of the character was investigated in F₂ and BC₁F₂ populations. The segregation ratios indicated that the shoot-lacking characteristics is expressed by double recessive gene pairs.

• Proceedings of the Korean Society of Tobacco Science Conference
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• 2001.05a
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• pp.35-43
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• 2001

일대잡종육종이란 현대의 다양한 육종기술 중 하나로 교배 제1세대의 잡종강세현상을 곧바로 이용하는 것으로 여러 작물에서 실용적으로 이용되고 있다. F$_1$잡종종자를 생산하려면 자가수분을 통제해야 하는데 웅성불임을 이용하면 자친의 화분제거 노력이 생력되어 경제적인 종자생산이 가능하다. 연초는 자식성 작물로 잡종강세는 높지 않으나 종간 교잡에 의한 cms가 만들어진 이후 버어리종 등에서는 60년대부터 직적형질에 가깝게 유전되는 우성 단일유전자의 지배를 받는 N. logniflora의 역병(race 0)저항성을 도입한 웅성불임(cms) F$_1$잡종이 활발하게 이용되어 왔다. 최근에는 cms F$_1$잡종의 이용영역이 넓어져 우리나라의 경우 N. glutinosa에서 유래한 우성 단일인자 TMV저항성을 도입한 황색종 cms F$_1$잡종 KF 114가 주 재배품종이 되었고 버어리종에서는 VAM에서 유래한 열성단일유전자의 지배를 받는 PVY저항성이 도입된 KB 111의 확대재배가 예상되고 있다. 본 글에서는 cms F$_1$잡종의 국내외 이용 사례를 살펴보고 cms F$_1$잡종의 육성과 이용방향, cms F$_1$잡종 종자생산에 관하여 기술하였다. 그리고 병저항성뿐 아니라 알칼로이드함량, 염록소함량, DVT나 챤-abienol 및 SE함량 등 질적형질에 가깝게 유전되고 유전양식이 명확하게 밝혀진 화학성분에 대해서도 F$_1$잡종 육성의 대상 형질로 논의하였다.

• The Korean Journal of Mycology
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• v.12 no.4
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• pp.164-169
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• 1984

Interspecific heterokaryons were obtained between auxotrophic mutants of Pleurotus ostreatus and Pleurotus florida by polyethylene $glycol-Ca^{++}$ induced fusion of somatic protoplasts. The fusion products produced colonies of dense growing mycelium after 10-14 days culture on hypertonic Mushroom Minimal Medium. When they were transferred to Mushroom Minimal Medium plates, the sectors showed normal vegetative morphology but the colonies of irregular shape varied in growth rate. All of the colonies produced fruit body of normal pilei. Some heterokaryon colonies bearing none or only a small amounts of basidiospores were isolated. Fusion products, generally, gave higher basidiocarp yields than the parents.

• Journal of Life Science
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• v.25 no.1
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• pp.16-20
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• 2015

During the storage of these two species in a large conservation tank, fertilized eggs were collected and the offspring were raised. During culturing of the offspring, individuals with mixed characteristics of these two species were observed, and 96 individuals were randomly tested using microsatellite markers applicable to both species. Among the 96 individuals, 15 individuals with mixed morphological characteristics were confirmed to be hybrids showing both of genotypes red seabream and blackhead seabream. Additionally, based on sequence analysis of mitochondrial cytochrome oxidase subunit 1 (mtDNA CO1), 81 showed 99% nucleotide sequence identity to that of black sea bream, and the remaining 15 individuals showed over 99% sequence identity to that of red seabream. So, hybrids were produced by female red seabream and male blackhead seabream. These results suggest that hybrids may form in nature between these two species if their habitats overlap due to the influence of humans or global climate change.

• Journal of Ginseng Research
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• v.27 no.4
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• pp.183-187
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• 2003

This study was carried out to ascertain the basic information on characteristics of Korean Ginseng(Panax ginseng) and American ginseng(Panax quinquefolius), F$_1$ hybrids. Interspecies hybrids between Panax ginseng and Panax quinquefolius were examined morphological characteristics, rusty root incidence, and contents of effective ingredients such as ginsenosides. The summarized results are as follows. In Panax ginseng, rusty root incidence tended to increase with age of ginseng, but there was no difference in the incidence among ginseng ages and cultivation years in Panax quinquefolius and F$_1$ hybrid. The interspecies hybrid of panax ginseng and Panax quinquefolius flowered later than the Panax ginseng, but earlier than the Panax quinquefolius. As for the characteristics of ginseng root, Panax quinquefolius seedling was better than cv. Panax ginseng, as the former had longer and heavier seedling root than the latter. Ginsenosides of the hybrid F$_1$ showed intermediate value in amounts of Rb$_1$, Rb$_2$, Rc and Rd which were detected as in Panax gineng and Panax quinquefolius. The amount of Re of the hybrid was higher, but that of Rg$_1$ and Rg$_2$ in main and branch roots was lower compared with its parents. Rf was 0.14% and 0.20% in main and branch roots of Panax ginseng, respectively; however, no Rf was detected in Panax quinquefolius and in the hybrid F$_1$. This suggests there may be remarkable difference in Rf content among the ginseng species.

• Korean Journal of Ichthyology
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• v.33 no.3
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• pp.157-166
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• 2021

Three specimens, presumed to be natural hybrids between Rhodeus notatus and R. ocellatus, were collected from Onyangcheon Stream area, Jwabu-dong, Asan-si, Chungcheongnamdo, the Republic of Korea, and morphological and phylogenetic analyses were performed to clearly identify their parent species. The body color of the three natural hybrids was light greenish-brown on dorsal side, and the size of red area on the upper front of the dorsal fin and the outer margin of the anal fin generally showed intermediate characters between the parent species, R. notatus and R. ocellatus. Among the measurement and meristic characters, the ratio of prepectoral length and preanal length in the standard length, and the ratio of snout length, interorbital width, length of caudal peduncle and depth of caudal peduncle in the head length, and the number of longitudinal row scales were analyzed as the unique characters of natural hybrids. In the rag1 gene of nuclear DNA, the three natural hybrids were analyzed to be reflected all the single nucleotide polymorphism sites between R. notatus and R. ocellatus, and in the phylogenetic tree using the cytb gene of mitochondrial DNA, they formed the same genetic clade as R. notatus. Therefore, three specimens, presumed to be natural hybrids analyzed in this study were identified as interspecific hybrids between female R. notatus and male R. ocellatus.

• Korean Journal of Plant Resources
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• v.28 no.1
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• pp.119-125
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• 2015

Interspecific crosses between rapeseed (Brassica napus L.) and chinese cabbage (B. campestris var. pekinensis Makino) and turnip (B. rapa L.) were made in order to examine the cross possibility and morphological phenotype of $F_1$ hybrid. The growth of pollen tube of cross between rapeseed and chinese cabbage was more rapid than cross between rapeseed and turnip. Silique formation and seed setting in silique were relatively high in the cross between rapeseed and chinese cabbage. The percentage of silique set from interspecific cross between rapeseed and chinese cabbage was 90.6% and higher 23.3% than the percentage of silique from interspecific cross rapeseed and turnip. The average number of seed per silique obtained from the cross rapeseed and chinese cabbage, and rapeseed and turnip reached 15.5 and 11.6, respectively. The morphological phenotypes of $F_1$ hybrid plants obtained from seeds in the cross between rapeseed and chinese cabbage resembled rapeseed mainly, but leaf length and leaf width were increased. The size, shape and lobation of leaves of $F_1$ hybrid plants from interspecific cross between rapeseed and turnip were intermediated between their parent species, but color of leaves was dark-green.

• FLOWER RESEARCH JOURNAL
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• v.17 no.4
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• pp.221-225
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• 2009

Interspecific crossing was conducted to obtain hybrid lilly with better quality of adapting to the unfavorable environment using oriental Lilium spp. as a female parent. Average interspecific crossing rate between oriental and asiatic lines by cut-style pollination was as low as 29%. Although the average germination rate of zygotic hybrid embryo between oriental 'Rodolfa' ${\times}$ asiatic 'Toronto' was increased from 76.6% to 78.3%. on 114 or 1/6 strength MS medium compared to the control, the rate of zygotic embryos formation between oriental 'Farolito' ${\times}$ asiatic 'Buff Pixie' was significantly enhanced up about 86~90% on 1/2 strength MS medium. Meanwhile, germination rate of interspecific hybrid embryo between oriental 'Snow Cristal' ${\times}$ asiatic 'Royal Trinity' showed up 100%. Germination rate of interspecific hybrids slightly increased by addition of $0.5-1.0mg{\cdot}L^{-1}$ GA. Germination rate of zygotic embryos between oriental 'Farolito' ${\times}$ asiatic 'Buff Pixie' and oriental 'Belcanto' ${\times}$ L. callosum was highest on the MS medium containing $0.01mg{\cdot}L^{-1}$ NAA and $0.1mg{\cdot}L^{-1}$ kinetin, as 66.6% and 45.2% respectively.

• Korean Journal of Ichthyology
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• v.24 no.1
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• pp.1-10
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• 2012

Reciprocal hybrids between the mud loach ($Misgurnus$ $mizolepis$) and cyprinid loach ($M.$ $anguillicaudatus$) were produced by artificial fertilization. The chromosome number of mud loach was 2n=48, consisting of 12M+4SM+32A chromosomes. The cyprinid loach has 2n=50, consisting of 10M+4SM+36A chromosomes. The chromosome numbers of the diploid reciprocal hybrids were 2n=49, consisting of 11M+4SM+34A chromosomes. All the karyotypes documented in this study had the same arm number of 64. There was no evidence of chromosomal polymorphisms or sex-related heteromorphism. The cytogenetic traits of the hybrid genotypes were intermediate between those of the parent species. In all genotypes, the chromosomal NORs localized to the terminal short arms of the same metacentric chromosome pair. These results suggest that Robertsonian translocation occurred between metacentric chromosome 1 of mud loach and acrocentric chromosome of cyprinid loach.

• Korean Journal of Ichthyology
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• v.27 no.1
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• pp.16-20
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• 2015

The hatchability of the artificially induced hybrid between two groupers (family Serranidae), red spotted grouper (Epinephelus akaara) and brown-marbled grouper (E. fuscoguttatus) that lives in different habit environment was investigated. There was no difference in the required time of each developmental stages after fertilization between hybrid (red spotted grouper ♀ ${\times}$ brown-marbled grouper ♂) and purebred (red spotted grouper ♀${\times}$♂) and required 25.6 hours to hatch at incubated in $25^{\circ}C$, but a noticeable unequal cleavage in cell size was observed in hybrid eggs unlikely to purebred. The hatching rate of fertilized eggs of hybrid was generally low across the four incubate temperatures (22, 25, 28, $31^{\circ}C$) with highest 9.8% in $25^{\circ}C$. This study demonstrated the possibility of artificial hybridization between two groupers, red spotted grouper and brown-marbled grouper, thus preparing the groundwork on developmental characteristics, deformities of hatched larvae and early survival ability for further studies on aquaculture.