• Journal of the Korean Chemical Society
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• v.54 no.5
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• pp.567-572
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• 2010

5,10-Methenyltetrahydrofolate synthetase from chicken liver was purified through 30-70% ammonium sulfate fractionation, Q Sepharose Fast Flow anion exchange and Source 15Phe hydrophobic interaction chromatography. Specific activities of cell extract, ammonium sulfate, Q Sepharose Fast Flow and Source 15Phe were 0.0085, 0.031, 0.80 and 1.27 U/mg, respectively. Purification fold activities of cell extract, ammonium sulfate, Q Sepharose Fast Flow and Source 15Phe were 1, 3.7, 94.1 and 149.4, respectively. HPLC gel permeation chromatography and SDS-polyacrylamide electrophoresis experiments indicated that the enzyme is a monomeric protein with a molecular weight of 22.8 kDa. Km for 5-methyl THF and Mg-ATP were $7.1\;{\mu}M$ and $63\;{\mu}M$, respectively. Optimum temperature and pH were $30^{\circ}C$ and 6.0, respectively. The data for metal ion specificity and stoichiometry showed that the maximum activity was obtained with a 1:l. ratio of $Mg^{2+}$. The ATP and Km values increased in the order of MgATP, MgCTP, MgUTP and MgGTP, and the maximum activities also decreased in the same order, indicating MgATP as the most efficient substrate. The enzyme was chemically modified only by tetranitrometane and 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimide, indicating that tyrosine and carboxylate are present in the active site.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.31 no.2D
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• pp.175-184
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• 2011

Transport sector takes charge of about 20 percent of energy consumption and GHG(Green House Gas) emission in Korea. One of the efficient strategy of reducing GHG is introducing CDM(Clean Development Mechanism), which is one of GHG reduction systems in Kyoto Protocol. Nowadays many tries have done to regist transport policies as CDM in transport sector, however, a lot of things should be investigated to regist CDM in advance. The aim of this paper is assessment of CDM possibility in transport sector. First of all, we review steps and criteria to CDM registration, and select 4 CDM possibility assessment index in transport sector: as follows additionality, methodology, emission calculation, and monitoring. Also, we analyze registed projects and methodologies in transport sector. To assess CDM possibility in transport sector, quantitative and qualitative assessments are carried out in this study. 18 transport policies are categorized as 4 groups and possibility of 18 transport policies are examined. Several policies can reduce GHG, however, they are not fit to regist as a CDM. On the contrary many transport policies have possibility to regist. In addition, we have done questionnaire survey, 'fuel change' policies have high possibility to CDM. However transport policies related to haman activity, like as TOD, have lower possibility. As a result, we can find that enough CDM possibility assessment should be carried out before CDM registration in transport sector.

• Korean Journal of Environment and Ecology
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• v.26 no.4
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• pp.475-483
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• 2012

Tribolodon hakonensis(Cypriniformes; Leuciscinae) is anadromous; they are born in freshwater, migrate back to the ocean, then return to their home stream for spawning from mid-March to early-June. Here, five microsatellites were used to assess the level of gene flow among T. hakonensis populations from the Samcheok-Oship Stream, South Korea. The frequencies of dominant alleles across several loci differed between down-and upstream populations divided by several weirs, and pairwise multilocus $F_{ST}$ estimate was significantly high(0.083). However, there were no signs of any loss of genetic variation in the upstream population. Assignment tests of individuals in admixture model(K=2) to a set of baseline samples showed fairly correct assignment to each cluster; all of upstream individuals sere included in the first cluster, while the majority of downstream individuals(65%) comprise the second cluster. These results indicate reduced gene flow between up- and downstream populations but allowing passive downstream drift. It is likely that man-made structures might at least partially be a factor for creating and consolidating the current distribution patterns of genetic variation among T. hakonensis populations in the Samcheok-Oship Stream. This information will assist governing agencies in making informed decisions regarding conservation of anadromous fishes in Korean drainage systems.

• Journal of the Korean Electrochemical Society
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• v.14 no.1
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• pp.1-8
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• 2011

Ionic liquids are room-temperature molten salts, composed of organic mostly of organic ions that may undergo almost unlimited structural variation. We approach the new aspects of ionic liquids in applications where the semiconductor nanoparticles used as sensitizers of solar cells. We studied the effects of ionic liquids as capping ligand and/or solvent, on the morphology and phase of the CdSe/ZnS nanoparticles. Colloidal CdSe/ZnS nanoparticles were synthesized using a series of imidazolium ionic liquids; 1-R-3-methylimidazolium bis(trifluoromethylsulfonyl) imide ([RMIM][TFSI]), where R = ethyl ([EMIM]), butyl ([BMIM]), hexyl ([HMIM]), octyl ([OMIM]). The average size of nanoparticles was 8~9 nm, and both zinc-blende and wurtzite phase was produced. We also synthesized the nanoparticles using a mixture of trihexyltetradecylphosphonium bis(trifluoromethylsulfonyl)imide ([$P_{6,6,6,14}$][TFSI]) and octadecene (ODE). The CdSe/ZnS nanoparticles have a smaller size (5.5 nm) than that synthesized using imidazolium, and with a controlled phase from zinc-blende to wurtzite by increasing the volume ratio of [$P_{6,6,6,14}$][TFSI]. For the first time, the phase and size control of the CdSe/ZnS nanoparticles was successfully demonstrated using those ionic liquids.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.41 no.1
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• pp.92-100
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• 2016

It is informed that the Voice over LTE(VoLTE) which serves voice and message on IP networks is better in terms of economies of scale than the legacy voice service on 2G/3G circuit-switched networks because of its technological and cost efficiency. In addition, services of voice and data are running on a single LTE network and as a result VoLTE has the more economies of scope. But, there is no study about how much technology-efficiency VoLTE has compared to circuit-based voice service and how much voice charge can be reduced as VoLTE grows up. This paper analyzes empirically cost-efficiency of VoLTE against circuit-based voice service and quantifies the reduction of voice charge as 2G/3G voice traffic shifts to VoLTE. The results describe the first is that the average cost of the total voice traffic rises shortly just after the investment of LTE network for providing VoLTE but it will soon have a capacity available to reduce the charge due to VoLTE's outstanding cost efficiency on the assumption that voice traffic is fixed, and the second is that the charge can be cut to 60% of the current rate in case of all the voice traffic moves to VoLTE. The latter proves partially the validation of data-focusing pricing plan. Our results are expected to become basic data for network operators' establishing pricing strategies and for policy makers' inducing price cutting.

• Cartoon and Animation Studies
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• s.39
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• pp.111-132
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• 2015

Although Rotoscoping technique has been used for a long time to mimic the natural and smooth motion since the early 20th century, its artistic value was devalued as tricks because it traces the already recorded images. But the fact that the rotoscoping images can cross the boundaries between animation and live action in an infinite integral freedom in the digital era became rather expansive new aesthetic possibilities of representation of the reality. In addition, Jonas Odell's animations such as (2010), (2008), (2006) are good example to prove that the rotoscoping images also can serve as means to enhance its narrative. This study is to analyze how rotoscoping images act as a unique role in relation with the narrative based on the said person's real stories and realistic images. I argue that his animated films constantly contain these three characteristics -Images to mediate Auditory sensitivity as a record of inner metaphysical reality, anonymous images to represent a specific existential character, and images that act as physical representation that holds the physical space/time and related memory. This reveals that rotoscoping images in this digital era went beyond reproduction for natural movements or special type of style. It rather suggests new layers of experience, and acquires new value in animation. I hope that this study could serve as a foundation to rediscover and re-position the value of rotoscoping images as well as good opportunity to introduce very talented swedish animation artist who already received global attention with his unique philosophical and aesthetic style.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.6 no.1
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• pp.124-132
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• 2002

In this paper, we present a vision algorithm and method for input image improvement and preprocessing of dented and raised characters on the sidewall of tires. we define optical condition between reflect coefficient and reflectance by the physical vector calculate. On the contrary this work will recognize the engraved characters using the computer vision technique. Tire input images have all most same grey levels between the characters and backgrounds. The reflectance is little from a tire surface. therefore, it's very difficult segment the characters from the background. Moreover, one side of the character string is raised and the other is dented. So, the captured images are varied with the angle of camera and illumination. For optimum Input images, the angle between camera and illumination was found out to be with in 90$^{\circ}$. In addition, We used complex filtering with low-pass and high-pass band filters to improve input images, for clear input images. Finally we define equation reflect coefficient and reflectance. By doing this, we obtained good images of tires for pattern recognition.

• Journal of Life Science
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• v.17 no.3 s.83
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• pp.350-355
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• 2007

The rat RBL-2H3 mast cells contain various Src-family kinases. Previous reports with this cell line indicated that Lyn activation is an important initial signaling for the activation of the cells. However, the role and location of other Src-family kinase isoforms which are expressed in the cells are not clear. In this study, we now show that isoforms of Src-family kinases, Lyn, fyn, Fgr, c-Src, and Yes are differentially expressed and located differently in the cells as indicated by RT-PCR, immunoblotting analysis, and confocal microscopy. Lyn and Fgr were located on plasma membrane but on the other hand c-Src and Yes were located on intracellular organelle. All of Src-family kinases were cloned and overexpressed for investigating the roles of the isoforms. Overexpression of Fyn and Fgr, not Lyn and c-Src, stimulated Ag-induced degranulation in the cells. Our findings strongly suggest for the first time that each of Src-family kinase isoform can regulate differentially $Fc{\varepsilon}RI$-mediated signaling in RBL-2H3 mast cells.

• Journal of KIISE:Information Networking
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• v.27 no.3
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• pp.301-314
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• 2000

As the number of World Wide Web (Web) users grows, Web traffic continues to increase at an exponential rate. Currently, Web traffic is one of the major components of Internet traffic. Also, high bandwodth usage due to Web traffic is observed during peak periods while leaving bandwidth usage idle during off-peak periods. One of the solutions to reduce Web traffic and speed up Web access is through the use of Web caching. Unfortunately, Web caching has limitations for reducing network bandwidth usage during peak periods. In this paper, we focus our attention on the use of a prefetching algorithm for reducing bandwidth during peak periods by using off-peak period bandwidth. We propose a statistical, batch, proxy-side prefetching scheme that improves cache hit rate while only requiring a small amount of storage. Web objects that were accessed many times in previous 24 hours but would be expired in the next 24 hours, are selected and prefetched in our scheme. We present simulation results based on Web proxy and show that this prefetching algorithm can reduce peak time bandwidth using off-peak bandwidth.

• Journal of Korean Philosophical Society
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• v.116
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• pp.281-312
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• 2010

Der vorliegende Beitrag problematisiert die Zeichen einer Zeit, in der nach der 'Wende des Sprachparadigmas' alle Prozesse einer Posttranszendenz, die in der heutigen philosophischen Problemsituation versucht werden, $bel{\ddot{a}}chelt$ werden, der Relativismus, Partikularismus und Skeptizismus $verst{\ddot{a}}rkt$ wird, und als Resultat die Kritik, Ablehnung und das Desinteresse an der Ideologie der Vernunft und Wahrheit sich verbreitet. Ich werde daher umgekehrt gerade an diesem Punkt ansetzen und ${\ddot{u}}ber$ die Notwendigkeit und Dringlichkeit der Rehabilitierung der "$Realit{\ddot{a}}t$ einer moralischen Vernunft" nachdenken. Zweck dieses Beitrags ist es, in Zusammenhang mit der Rehabilitierung der $Realit{\ddot{a}}t$ einer moralischen Vernunft, aufzuzeigen, welche theoretische Erkenntnis bzw.moralische Erkenntnis wahr ist, d.h., dass ein 'bewiesenes', da 'objektiv gerechtfertigtes' Argument existiert. $Daf{\ddot{u}}r$ werde ich als erstes auf das Problem der Relativistischen und Skeptizistischen Anhaltspunkte philosophisch eingehen(2), zur ${\ddot{U}}berwindung$ dieser Anhaltspunkte eine Rechtfertigung der rationelen und moralischen $Begr{\ddot{u}}ndung$ versuchen(3). In Folge soll betrachtet werden, dass die Rechtfertigung der rational moralischen $Begr{\ddot{u}}ndung$ mit dem Problem der $Letztbegr{\ddot{u}}ndung$ in enger Verbindung steht(4), und nachdem darauf hingewiesen wird, dass dies durch eine Konfrontation mit der Fallibilismus These zu erkenntnistheoretisch objektiver $G{\ddot{u}}ltigkeit$ gelangt(5), werde ich den Beitrag $abschlie{\ss}en$(6).