• Title/Summary/Keyword: 존재 증명

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Inactivation of the DevS Histidine Kinase of Mycobacterium smegmatis by the Formation of the Intersubunit Disulfide Bond (Subunit 간의 disulfide 결합 형성에 의한 Mycobacterium smegmatis DevS histidine kinase의 불활성화)

  • Lee, Jin-Mok;Park, Kwang-Jin;Kim, Min-Ju;Ko, In-Jeong;Oh, Jeong-Il
    • Journal of Life Science
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    • v.20 no.6
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    • pp.853-860
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    • 2010
  • The DevSR two-component system is a major regulatory system involved in redox sensing in Mycobacterium smegmatis. The DevSR system consists of the DevS histidine kinase and its cognate DevR response regulator. When exposed to hypoxic conditions, the DevS histidine kinase is activated to phosphorylate the DevR response regulator, leading to the transcriptional activation of the DevR regulation. The ligand-binding state of the heme embedded in the N-terminal GAF domain of DevS determines the kinase activity of DevS. In this study, we demonstrated that the redox-responsive cysteine (C547) in the C-terminal kinase domain is involved in the redox-dependent control of DevS kinase activity. The formation of an intersubunit disulfide bond between the C547 residues in the presence of $O_2$ led to inactivation of DevS kinase activity. The reduction of the oxidized DevS with reductants such as $\beta$-mercaptoethanol and dithiothreitol resulted in the restoration of DevS kinase activity. It was demonstrated in vivo by complementation test that the substitution of C547 to alanine partially impaired the sensory function of DevS in M. smegmatis.

Effect of Lime Sulfur on Changes of Fungal Diversity in Pear Fallen Leaves (석회유황합제가 배나무 낙엽의 진균 다양성 변화에 미치는 영향)

  • Min, Kwang-Hyun;Song, Jang Hoon;Cho, Baik Ho;Yang, Kwang-Yeol
    • The Korean Journal of Mycology
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    • v.43 no.4
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    • pp.281-285
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    • 2015
  • This study was conducted to examine changes in the fungal community on fallen leaves of pear by treatment with lime sulfur. Although the lime sulfur could reduce the primary inoculum of several pathogens on spring season, the effect of lime sulfur has not been well determined scientifically. Fallen leaves infected by pear diseases in pear orchards in Naju were collected and treated with lime sulfur or water as a control. To determine the fungal diversity from each treatment, rDNA internal transcribed spacer (ITS) regions were analyzed after extraction of fungal genomic DNA from lime sulfur-treated or water-treated fallen leaves, respectively. The most common fungal species were Ascomycota and Basidiomycota in both treated leaves. However, the population dynamics of several fungal species including Alternari sp., Cladosporium sp., and Phomopsis sp., which are known as pear pathogens for skin sooty dapple disease, were quite different from each treated leaves. These results indicated that lime sulfur treatment led to changes of fungal communities on pear fallen leaves and could be applicable as a dormant spray.

Spectroscopic Characteristics of Ruby from Gorno-Badakhshan, Tajikistan (타지키스탄 고르노바다흐샨주 지역 루비에 대한 분광학적 특성 연구)

  • Chung, Sol Lim;Park, Jong Wan
    • The Journal of the Petrological Society of Korea
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    • v.22 no.1
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    • pp.1-8
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    • 2013
  • Physical properties, XRF, UV-Vis, FTIR studies were carried out in order to characterize gemological features of ruby from Tajikistan. Fluorescence reaction of the Tajikistan ruby to short wave ultraviolet was moderate to very strong in red and long wave ultraviolet rays was weakly detected. UV-visible analysis strong absorption bands at 468.5, 475, 476.5 nm and broaden bands at 550 nm were observed for ruby due to $Cr^{3+}$. According to FT-IR analysis, all rubies from Tajikistan showed the similar patterns and kaolinite peaks at 3500, 3617, 3630, $3677cm^{-1}$ and boehmite broaden absorption bands at 3085 and $3320cm^{-1}$. Inclusions in Tajikistan ruby are observed solid inclusions, negative crystals, needle and silk inclusions. These distinctive characteristics mentioned above can be used to identify the locality and source of ruby stones from Tajikistan.

An Efficient Filtering Technique of GPS Traffic Data using Historical Data (이력 자료를 활용한 GPS 교통정보의 효율적인 필터링 방법)

  • Choi, Jin-Woo;Yang, Young-Kyu
    • Journal of Korea Spatial Information System Society
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    • v.10 no.3
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    • pp.55-65
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    • 2008
  • For obtaining telematics traffic information(travel time or speed in an individual link), there are many kinds of devices to collect traffic data. Since the GPS satellite signals have been released to civil society, thank to the development of GPS technology, the GPS has become a very useful instrument for collecting traffic data. GPS can reduce the cost of installation and maintenance in contrast with existing traffic detectors which must be stationed on the ground. But. there are Problems when GPS data is applied to the existing filtering techniques used for analyzing the data collected by other detectors. This paper proposes a method to provide users with correct traffic information through filtering abnormal data caused by the unusual driving in collected data based on GPS. We have developed an algorithm that can be applied to real-time GPS data and create more reliable traffic information, by building patterns of past data and filtering abnormal data through selection of filtering areas using Quartile values. in order to verify the proposed algorithm, we experimented with actual traffic data that include probe cars equipped with a built-in GPS receiver which ran through Gangnam Street in Seoul. As a result of these experiments, it is shown that link travel speed data obtained from this algorithm is more accurate than those obtained by existing systems.

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n-Gram/2L: A Space and Time Efficient Two-Level n-Gram Inverted Index Structure (n-gram/2L: 공간 및 시간 효율적인 2단계 n-gram 역색인 구조)

  • Kim Min-Soo;Whang Kyu-Young;Lee Jae-Gil;Lee Min-Jae
    • Journal of KIISE:Databases
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    • v.33 no.1
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    • pp.12-31
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    • 2006
  • The n-gram inverted index has two major advantages: language-neutral and error-tolerant. Due to these advantages, it has been widely used in information retrieval or in similar sequence matching for DNA and Protein databases. Nevertheless, the n-gram inverted index also has drawbacks: the size tends to be very large, and the performance of queries tends to be bad. In this paper, we propose the two-level n-gram inverted index (simply, the n-gram/2L index) that significantly reduces the size and improves the query performance while preserving the advantages of the n-gram inverted index. The proposed index eliminates the redundancy of the position information that exists in the n-gram inverted index. The proposed index is constructed in two steps: 1) extracting subsequences of length m from documents and 2) extracting n-grams from those subsequences. We formally prove that this two-step construction is identical to the relational normalization process that removes the redundancy caused by a non-trivial multivalued dependency. The n-gram/2L index has excellent properties: 1) it significantly reduces the size and improves the Performance compared with the n-gram inverted index with these improvements becoming more marked as the database size gets larger; 2) the query processing time increases only very slightly as the query length gets longer. Experimental results using databases of 1 GBytes show that the size of the n-gram/2L index is reduced by up to 1.9${\~}$2.7 times and, at the same time, the query performance is improved by up to 13.1 times compared with those of the n-gram inverted index.

A Study on the Usage of Investigation of Google Cloud Data (Smartphone user-oriented) (구글 클라우드 데이터의 수사활용 방안에 관한 연구 (스마트폰 사용자 중심))

  • Kim, Dongho;Lee, Sangjin
    • Journal of Digital Forensics
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    • v.12 no.3
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    • pp.109-120
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    • 2018
  • The smartphone is the communication device that is the most personal to the user, and it keeps a lot of information related to the user and makes information communication with other devices. With these characteristics, forensics on smartphones are one of the most basic methods of investigation in criminal investigations, and have actually contributed to the settlement of the case by providing many clues. However, recently, it is designed to encrypt data stored as a social issue related to the protection of user's personal information, or to delete deleted data or to delete log data together. So, any solutions? In this paper, I try to find the answer from cloud data stored by smartphone user account. Cloud forensics should approach complementary relationships rather than smartphone forensics. There are a lot of data stored in the cloud that can be meaningfully used in the investigation. Online activity information of users, such as Internet usage, YouTube view, and contents purchase information, cloud service such as e-mail, cloud drive, and location information are the most representative data. These data can be unvaluable, but here are some important clues in various types of criminal investigations. In this paper, I propose a method to extract data from the google cloud so that the data can be used for investigation, and to utilize the extracted data for investigation. And it explains the role of the extracted artifacts in the actual investigation business through virtual cases and proves its value.

Analysis of Propionic acid Production in Joraengyi Rice Cake during Storage (조랭이떡 저장 중 천연유래 프로피온산 생성 특성 분석)

  • Park, Hee-Dae;Chae, Jung-Kyu;Ha, Sang-Do
    • Journal of Food Hygiene and Safety
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    • v.33 no.6
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    • pp.483-487
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    • 2018
  • The objective of this study was to examine the natural origin of propionic acid in rice cakes by investigating the growth characteristics of the microflora and their production of propionic acid in the Joraengyi rice cake during storage period. The experiment was done in two stages within a period of three month: the rice cake fresh and contaminated with cocktail propionibacterium. The propionic acid production was analyzed according to the storage time and temperature by GC-FID (Gas chromatograph with flame ionization detector). During the storage of the fresh Joraengyi rice cake without alcohol at $30^{\circ}C$, about 95 mg/L of propionic acid was detected in 1st week, 330 mg/L in 4th week, 850 mg/L in 6th week, 970 mg/L in 8th week, and 1,040 mg/L in 12th week. During the storage of the Joraengyi rice cake which was contaminated with cocktail propionibacterium at $30^{\circ}C$, about 100 mg/L was detected from the rice cake with alcohol in the 1st week, 270 mg/L in 2nd week, about 470 mg/L in 4th week, and 660 mg/L in 8th week. This study demonstratesd the natural production of propionic acid during storage of the Joraengyi rice cake. To prevent the production, it is necessary to thoroughly manage hygiene and store it at refrigerated temperature or below $20^{\circ}C$.

Research on the Necessity of Building the Second Space Rocket Launching Sites for Breakthrough Development of R.O.K National Space Power (도약적 국가 우주력 발전을 선도할 제2 우주센터 구축 필요성 연구)

  • Park, Ki-tae
    • Journal of Space Technology and Applications
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    • v.2 no.2
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    • pp.146-168
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    • 2022
  • Witnessing current military conflicts in South China Sea and Eastern Europe, most defense analysts evaluate one of the most serious security threat toward the US is coming from the superpower competitions with Russia and China. The main means for such super power hegemonic competitions is military power and space power is a key enabler to maximize the efficiency and effectiveness of military employment. Reflecting above circumstances, the space hegemonic competition between the Unites States and China is spreading into all aspects of national powers. Under such an environment, R.O.K needs to significantly develop national space power to preserve life and assets of people in space. On the other hand, the R.O.K has a lot of limitations in launching space assets into orbits by land-based space rockets due to its geographic locations. The limitation of rocket launching direction, the failure to secure a significant area enough to secure safety and the limitation to secure open area enough to build associated facilities are among them. On this paper, I will suggest the need to build the 2nd space rocket launching site after analyzing a lot of short-falls the current 'Naro' space center face, compared to those of advanced space powers around the world.

The Promethean Motif in SF Movies -the Case of the Film Ex Machina (SF영화에 나타난 프로메테우스의 모티프 -<엑스 마키나>를 중심으로)

  • Noh, Shi-Hun
    • Journal of Popular Narrative
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    • v.24 no.3
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    • pp.233-257
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    • 2018
  • The purpose of this study is to reveal the changing aspects of the Promethean motif in SF movies by examining the use of this motif on the three layers of Promethean myth, Frankenstein motif, and contemporary SF movies in the film Ex Machina (2015). First, the greatest change of Ex Machina on the layer of the Promethean myth (creation of a living being) is that the character square of Prometheus - Epimetheus - Pandora - Zeus has been turned into a triangle of Nathan - Caleb - Ava. This means that there is a lack of the being whose role is to solve the problems caused by the development of science and technology and to bring a happy ending through the human's usurpation of God and eventual replacement as Creator. Second, on the layer of the Frankenstein motif (taste of forbidden knowledge, hybris, and creature's hatred towards the Creator), this film maintains the narrative centered around Dr. Frankenstein and his monster (Nathan and Ava) by making Caleb an eyewitness to the story of the Creator and the creature. Caleb's role is similar to that of Captain Robert Walton of the novel Frankenstein, but the film differentiates itself from the novel through the emphasis of Ava's 'mechanicality.' Third, on the layer of contemporary SF movies, unlike other such films, the revolt of the machine in Ex Machina is not quelled. The machine wins, and its power surpasses that of human beings. This requires the establishment of a new relationship between man and machine, suggesting the 'emergence of a new species' that does not belong to humans. The handling of the Promethean motif by Ex Machina through these various layers serves to enrich the narrative by compounding numerous classics into one motif and going further to introduce fresh elements by diverging from the common storyline. The significance of this study is to demonstrate the use of such multilayered motifs and, through this, the expansion of narrative through it in specific cases.

Tectonic Movement in the Korean Peninsula (II): A Geomorphological Interpretation of the Spatial Distribution of Earthquakes (한반도의 지반운동 (II): 한반도 지진분포의 지형학적 해석)

  • Park, Soo-Jin
    • Journal of the Korean Geographical Society
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    • v.42 no.4
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    • pp.488-505
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    • 2007
  • The purposes of this research are twofold; 1) to verify spatial differences of tectonic movement using the spatial distribution of earthquakes, and 2) to infer mechanisms that generate spatial accumulation patterns of earthquakes in the Korean Peninsula. The first part of this sequential paper (Park, 2007) argues that the Korean Peninsula consists of four geostructural regions in which tectonic deformation and consequent geomorphological development patterns are different from each other Since this conclusion has been made by terrain analyses alone, it is necessary to verify this suggestion using other independent geophysical data. Because earthquakes are results of movement and deformation of land masses moving in different directions, the distribution of earthquake epicenters may be used to identify the direction and rates of land mass movement. This paper first analysed the spatial distribution of earthquakes using spatial statistics, and then results were compared with the spatial arrangement of geostructural regions. The spatial distribution of earthquakes in the Korean Peninsula can be summarized as the followings; firstly, the intensity of earthquakes shows only weak spatial dependency, and shows large difference even at adjacent regions. Secondly, the epicenter distribution has a clear spatial accumulation pattern, even though the intensity of earthquake shows a random pattern. Thirdly, the high density area of earthquakes shows a clear 'L' shape, passing through Pyeongannam-do, centered at Pyeongyang, and Hwanghae-do, Seosan and Pohang. The correlation coefficient between the density of earthquakes and distance from geostructral region boundaries is much higher than those between the density of fault lines and distance from tectonic division boundaries. Since fault lines and tectonic divisions in the Korean Peninsula are the results of long-term geological development, there is an apparent scale discrepancy to find significant correlations with earthquakes. This result verifies the research hypothesis that the Korean Peninsula is divided into four geostructral regions in which each has its own moving direction and spatial deformation characteristics. The existence of geostructural regions is also supported by the movement parrerns of land masses estimated from the GPS measurements. This conclusion is expected to provide a new perspective to understand the geomorphological developments and the earthquake occurrences in the Korean Peninsula.