• Microbiology and Biotechnology Letters
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• v.32 no.4
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• pp.322-327
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• 2004

A strong constitutive PJH promoter from Bacillus sp. was applied to overexpress the endoxylanase gene (639 bp) in Bacillus subtilis. The expression plasmid, pJHKJ4, was designed to contain the $P_{JH}$ promoter and open reading frame of endoxylanase including its own promoter. The plasmid was introduced into B. subtilis DB431 and the resulting transformant was grown on LB glucose medium. At the end of cultivation, the endoxylanase activity in the culture supernatant reached about 140 DIm!. The enzyme in the supernatant was concentrated by ultrafiltration (MW cut-off 10 kDa and 30 kDa) and ammonium sulfate precipitation. For the concentration of the enzyme, ultrafiltration was more efficient than 70% ammonium sulfate precipitation. The stabilization of concentrated enzyme solution at $50^{\circ}C$ was examined with various stabilizers such as NaCI, glycerol, polyethylene glycol, sorbitol, and $CaCI_2$. The most effective stabilizers were found to be NaCI and $CaCI_2$.

• Journal of Korea Soil Environment Society
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• v.4 no.2
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• pp.137-147
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• 1999

Fluidized-bed thermal desorber coupled with a heat pipe was investigated for the remediation of soil contaminated with diesel oils. Thermal gravimetric analysis by Cahn-balance indicated that the desorption of diesel oils from the soil particles was mainly governed by the internal diffusion at low concentration of less than 0.5 wt. % of oils in the soil particles. In fluidized-bed experiments. increase of fluidizing gas velocity reduced the residual oils of the contaminated soils, the increase of soil feed rate decreased efficiency of fluidized-bed desorber. A mathematical model was developed by incorporating Fickian diffusion kinetics into the Kunii-Levenspiel model Simulation results showed reasonable agreement for the performance of fluidized-bed thermal desorber.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.7 no.3
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• pp.161-165
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• 2009

To evaluate the efficiency of the decontamination plant for the removal of uranium compounds deposited on the internal surface of $UF_6$ cylinder for its reuse, two demonstration tests of the plant with different ratio of ${Na_2}{CO_3}$ and ${H_2}{O_2}$ were carried out, and each test had 5 steps. The main chemical form removed by the tests was to be identified as ${Na_4}{UO_2}(CO_3)_3$. More than 50% of uranium was removed by water of the first step, and at the following steps the removal amounts were exponentially decreased. On the other hand, the result shows that the injected amount of ${Na_2}{CO_3}$, compared with that of the removed uranium, was stoichiometrically excessed. This suggests that the injected amounts of ${Na_2}{CO_3}$, the generation rate of decontaminated waste, and the decontamination steps could be reduced by a process optimization of the plant.

• Journal of Sensor Science and Technology
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• v.9 no.1
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• pp.70-75
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• 2000

The recycled single crystal silicon wafers have been fabricated into solar cells. It can be a solution for the high cost in materials for solar cells and recycling of materials. So, p-type (100) single crystal silicon wafers with high resistivity of $10-14\;{\Omega}cm$ and the thickness of $650\;{\mu}m$ were used for the fabrication of solar cells. Optimistic conditions of formation of back surface field, surface texturing and anti-reflection coating were studied for getting high efficiency. In addition, thickness variation of solar cell was also studied for increase of efficiency. As a result, the solar cell with efficiency of 10% with a curve fill factor of 0.53 was fabricated with the wafers which have the area of $4\;cm^2$ and thickness of $300\;{\mu}m$. According to above results, recycling possibility of wasted wafers to single crystal silicon solar cells was confirmed.

• Applied Chemistry for Engineering
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• v.24 no.1
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• pp.31-37
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• 2013

Lowering surface reflectance of Si wafers by texturization is one of the most important processes for improving the efficiency of Si solar cells. This paper presents the results on the effect of texturing using acidic solution mixtures containing the catalytic agents to moderate etching rates on the surface morphology of mc-Si wafer as well as on the performance parameters of solar cell. It was found that the treatment of contaminated crystalline silicon wafer with $HNO_3-H_2O_2-H_2O$ solution before the texturing helps the removal of organic contaminants due to its oxidizing properties and thereby allows the formation of nucleation centers for texturing. This treatment combined with the use of a catalytic agent such as phosphoric acid improved the effects of the texturing effects. This reduced the reflectance of the surface, thereby increased the short circuit current and the conversion efficiency of the solar cell. Employing this technique, we were able to fabricate mc-Si solar cell of 16.4% conversion efficiency with anti-reflective (AR) coating of silicon nitride film using plasma-enhanced chemical vapor deposition (PECVD) and Si wafers can be texturized in a short time.

• KSBB Journal
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• v.14 no.4
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• pp.476-481
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• 1999

S. cerevisiae mutant(reg1-501, gal1), which cannot use galactose and has alleviated glucose repression level, is used as host for optimizing induction of GAL promoter. The optimum concentration of galactose as inducer for recombinant protein production and the galactose consumption rate have been tested with S. cerevisiae mutant and compared with conventional S. cerevisiae. The extent of glucose repression were investigated for both strain and the degradation pattern of produced foreign protein have been compared in both cases. The effect of pH on foreign protein degradation pattern were studied for both strains. The secetion efficiency of both strains were carried out. Through these experiments, optimum condition of recombinant protein production by GAL promoter using S. cerevisiae mutant (reg1-501, gal1) were found.

• Journal of the Korea Society for Simulation
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• v.15 no.4
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• pp.119-128
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• 2006

A turnout system which permits trains to pass from one track to another is a combination of the switch, the crossing, lead rails which are necessary to connect the switch and the crossing, two guard rails and a switch machine for operating the switch. A turnout is the sole moving part among the railway components and has complex configuration, so the safety has always been raised an issue. In Korea, it is planned to adopt the high speed tilting train, which operates at the maximum speed of 200km/h, at conventional lines by the year of 2010. However, for the application of the tilting train to conventional lines, it is prerequisite to establish a stable turnout system allowing the tilting train to pass through it without reducing speed. Therefore, the improved turnout system for the speed-up of conventional lines has been developed and the prototype of the turnout system has been constructed. In this study, simulation of noise and vibration around the improved turnout system was performed in order to predict the generation level of noise and vibration due to passing of the tilting train through the turnout system.

• Korean Journal of Optics and Photonics
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• v.18 no.1
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• pp.1-7
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• 2007

Filter colorimeters have a set of spectral bands for which spectral responsivity is the same as the color matching function defined by CIE (Commission Internationale de I'Eclairage). We have designed a set of color matching function filters denoted by $\bar{x}-filter,\;\bar{y}-filter,\;and\;\bar{z}-filter$. Because the $\bar{x}-function$ has two transmission bands, two $\bar{x}-filters$ are designed to cover the $\bar{x}-function$. To design the filters, we developed a nonlinear least square fit program which determines the thickness of the color glasses by minimizing its spectral mismatch value ($f{_1}'$) to below 3 %. The design has been validated by fabrication of the $\bar{y}-bar$ filter, of which $f{_1}'$ was measured to be 2.8 %. Considering a LCD flat panel display as a device under test, we have calculated the systematic error of the colorimetric measurement using the designed filters.

• Journal of Korean Society of Forest Science
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• v.85 no.2
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• pp.172-179
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• 1996

This paper described the application of fuzzy multiobjective linear programming to solving a multiple-use problem of forest resources management. At first the concepts of linear programming, fuzzy linear programming and fuzzy multiobjective linear programming were introduced briefly. In order to illustrate a role of fuzzy multiobjective linear programming in the process of multiple-use forest planning, the natural recreation forest in Mt. Yoomyung was selected for this study. A fuzzy multiobjective linear programming model is formulated with data obtained from this Mt. Yoomyumg natural recreation forest to solve the multiple-use management planning problem of forest resources. Finally, the results, which were obtained from the calculation of this model, were discussed. The maximal value of the membership function(${\lambda}$) was 0.29, when the timber production and the forest recreation function were optimized at the same time through the fuzzy multiobjective linear programming. The cutting area in each period was 102.7ha, while total cutting area was 410.8ha for 4 periods. During 4 periods $57,904m^3$ will be harvested from this natural recreation forest and at the same time total visitors were estimated to be about 8.6 millions persons.

• Geophysics and Geophysical Exploration
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• v.12 no.4
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• pp.289-298
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• 2009

Optimal data processing parameters were designed to attenuate multiples in seismic data acquired in the south-eastern area of the East Sea, in 2008. Bunch of multiples caused by shallow sea water depth were perceived periodically up to two way travel time of 1,750 ms at every 250 ms over seismic traces. We abbreviated sea bottom multiple as SBM, Peg-leg multiple as PLM, and free-surface multiple as FSM. To attenuate these multiples, seismic data processing flow was constructed including NMO, stack, minimum phase predictive deconvolution filter and wave equation multiple rejections (WEMR). Prevalent multiples were suppressed by predictive deconvolution and remaining multiples were attenuated by WEMR. We concluded that combining deconvolution with WEMR was effective to a seismic data of study area. Derived parameter can be applied to the seismic data processing on adjacent survey area.