• Korean Journal of Ichthyology
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• v.28 no.1
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• pp.9-18
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• 2016

Histological and morphological development of the digestive tract of sevenband grouper were observed from after hatching to 60 days. Fishes were fed with rotifer (Brachionus rotundiformis) and chlorella (Chlorella ellipsoidea) after hatching from 2 to 20 days rotifer and brine shrimp (Artemia salina) in after 20 days rotifer, brine shrimp and semi-dry artificial diet in after 23 days. Histological and morphological development of ten larvae was observed by paraffin embedding method after fixing in 10% neutral buffered formalin. Sevenband grouper RLG showed characteristics of carnivorous fish by average 0.87. Larvae after hatching can't open the mouth and anus digestive tract was observed in a straight line following yolk sac. Larvae was observed feeding activity by opening the mouth and anus. Metamorphosis started 8 days after hatching. Esophagus divided four layer, and goblet cell was observed in esophagus, mid intestine and rectum. Larvae started cannibalism and it was caused by difference of growth. The inside of stomach was differentiated to cardiac orifice, body of stomach, pyloric stomach, and pyloric caeca. Goblet cell was observed all intestine. Gastric gland differentiated after hatching 28 days in stomach. Secretion of gastric juice was found at stomach and mucosal fold pyloric caeca. Even thought the inside of stomach expended and the number of gastric gland increased consistently and goblet cell in intestine and mucosa became longer, histochemical changes follow couldn't be found during transforming juveniles 38 days after hatching.

• Journal of Chest Surgery
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• v.32 no.12
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• pp.1078-1086
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• 1999

배경 :폐포내 fibronectin(FN)의 분포와 역할은 많은 연구자에 의하여 연구되어왔다. 흰주에서 폐의 분화시 FN은 태자에서 폐포의 기저막에 주로 분포되고 간엽조직에서도 관찰되면 분화가 진행되면 폐포막의 간질조직에 FN의 함량이 높아진다. 또 FN은 일반적으로 폐포대식세포(alveolar macrophage)에서 분비되고 폐에 질병이 발생하였을 때 다량의 FN이 폐포대식세포에서 분비된다고 보고되어 있다(Schoenberger 등 1984: Ozaki 등 1990; Rom 등 1987 ; Cordier 등 1990) 저자는 흰쥐의 폐포발생이 진행중인 폐포기 후반에서의 폐포막내 정상적인 FN이니 분포의 변화와폐포를 구성하는 큰 폐포세포(type II pneumocyte)에서의 FN의 분비여부를 면역조직염색법과 전자현미경을 이용하여 추적하고자하였다 실험대상 및 방법: 청정동물실에서 사육한 SPF 흰쥐(Sprague-Dawley 계)를 임신시켜 질도말법을 이용하여 태령을 정한 뒤 태아제 17일 및 20일 출생 제 1일, 2일, 3일, 5일, 및 7일의 신생흰쥐를 실험동물로 사용하였으며 대조군의 흰쥐는 체중 200ㅎ의 건강한 수컷을 사용하였다 흰쥐의 폐조직은 면역조직염색을 위해 rabbit anti rat fibronectin polyclonal antibody를 일차항체로 biotinylated goat anti rabbit IgG를 이차항체로 사용하여 폐실질세포내 FN의 분포를 LM으로 관찰하였고 한편 폐포막을 구성하는 세포 중 큰폐포세포가 FN을 분비하는 세포인지를 추적하기 위해 금과립을 첨가한 항체를 사용하여 큰 폐포세포내 FN의 분포를 EM을 이용해서 추적한 결과 다음과 같은 결과를 얻었다 결과 : 제 17일 및 20일 태아시기의 폐에서의 혈관주위에 강한 FN반응이 관찰되었다 출생후 폐포막의 FN의 활성은 출생후 5일 및 7일에 최고주에 달했다. 출생직후 1-2일경에 혈관의 조직내 FN의 활성이 양성을 나타내지만 3일이후 활성이감소되었다. 폐포대식세포내 FN의 활성은 출생후 증가되었다. 폐조직내 소기관지의 FN의 활성은 출생후 완만하게 상승되었다. 큰 폐포세포는 출생 1-3일에 일정량의 FN 반응이 세포질과 미세융모내에 관찰되었다. 결론 : 이상과 같은 결과로 흰쥐의 폐포의 분화과정이 계속되는 출생후 폐에서 FN의 분비는 7일이내에 성숙흰쥐의 폐포내 반응과 비슷한 반응으르 보이며 이때 폐의 실질조직은 분화가 거의 완료되었을 것으로 사료되었고 큰 폐포세포에서도 FN이 분비되는 것으로 결론지울수 있다.

• Journal of Yeungnam Medical Science
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• v.3 no.1
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• pp.33-39
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• 1986

A clinical and statistical study was done for 123 patients with histologically proven colorectal malignant tumor from 1983 to 1986 at the department of anatomical pathology. Yeung Nam University Hospital. The results were as follows: 1. Ratio between male and female was 1.6:1 and incidence was most prevalent in 7th decades comprising 29.3%. 2. Location of tumor was the most frequent in rectum(65%). 3. Frequent symptoms and signs in case of right colon were pain, abdominal mass and bowel habit change. In left colon, they were pain, bowel habit change and bloody stool or melena. In rectum, they were bloody stool or melena, bowel habit change and pain. 4. Duration of symptom was 1 to 3 months(33.3%). 5. The most frequent histological type was adenocarcinoma(82.9%). 6. According to Dukes's classification, 32.9% of the tumor were stage $C_2$. 7. Operative procedures were Mile's operation(47.0%), right hemicolectomy(19.8%) and lower anterior resection(11.7%). 8. Polyps were the most frequent associated disease. 9. The most common complication was wound infection(11.1%).

• The Journal of the Korean bone and joint tumor society
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• v.18 no.1
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• pp.20-27
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• 2012

Purpose: The chemokine receptor CXCR4 has been reported to be aberrantly expressed in human cancer and has been shown to participate in cancer metastasis. We compared the expression of CXCR4 in conventional high-grade and low-grade central osteosarcomas, and determined if an association between CXCR4 expression and prognosis could be made. Materials and Methods: We performed the immunohistochemistry for CXCR4 in a total of 63 patients with osteosarcoma and determined the relationships according to the clinicopathologic variables and overall survival rates. Results: CXCR4 was detected in 76.3% of conventional high-grade osteosarcoma patients and in 36% of low-grade central osteosarcomas. Diffuse expression was noted in 47.4% of the high-grade osteosarcomas and all low-grade cases were focal positive. CXCR4 expression was significantly correlated with histologic grade (p<0.0001). While overall survival rate was reduced significantly with increased CXCR4 expression (p=0.0058), higher histologic grade (p<0.0001), and younger age (p=0.0140), survival rate did not correlate with gender, tumor size, or AJCC stage. Conclusion: Our results suggest that CXCR4 expression is associated with higher-grade tumors and with poor prognosis for osteosarcoma patients.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.05a
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• pp.165-166
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• 2000

은어는 암컷의 성장이 수컷보다 빠르고, 성숙 시 수컷의 상품가치가 크게 하락하므로 실제 양식 현장에서는 전 암컷 종묘의 생산이 절실히 요구되고 있다(방 등, 1997). 호르몬에 의한 암컷 종묘의 생산방법이 연구되었으나 암컷 유도율이 매우 낮고, 성전환된 수컷을 친어로 이용하여 단순교배에 의한 전 암컷 집단을 생산하는 유전학적 성전환 방법이 아직 확립되지 않았다(고교, 1993).유전학적 성전환을 위해 웅성호르몬 처리에 의한 phenotypic male (genetic female)을 생산하여야 하는데 본 종은 수컷으로의 성전환이 매우 어려운 종으로 알려지고 있다. 따라서 정확한 성전환 처리 방법을 구명하기 위해서 우선 성 분화 과정을 조직학적 방법으로 조사하는 것이 필요하다. (중략)

• Proceedings of the KTCVS Conference
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• 1998.10a
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• pp.11-11
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• 1998

• Journal of Life Science
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• v.16 no.7 s.80
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• pp.1141-1147
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• 2006

This study was carried out to establish a reproducible culture system for callus formation and adventitious shoot development from young stem segments of Vigna radinta, and histological work for orgin of callus tissue and adventitious shoot. Induction of callus from young stem explants of Vigna radiata was very effective on MS inorganic salts supplemented with 0.5 mg/L 2,4-D and 1.0 mg/L kinetin. For the adventitious shoot regeneration from the callus tissues, the hormone combination of 0.75 mg/L NAA, 1.5 mg/L kinetin and MS salts resulted in about 21% efficiency. Histological examination showed that callus tissues originated from out-growths by callus cambium rings with do novo meristematic activities, which were localized at the outside of the vascular cambium. Adventitious shoots were developed from shoot apical meristem originated from the surface of callus masses. The shoot apical meristem produced leaf primordium, which then became leaf.

• Proceedings of the KOR-BRONCHOESO Conference
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• 1993.05a
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• pp.83-83
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• 1993

The nuclear phosphoprotein p53 is expressed in all normal cells and appears to function in cell cycle regulation. Abnormally high levels of the protein are found in many different types of cancer. In human cancer overexpression of p53 is associated with point mutations within highly conserved regions of p53 gene. These altered genes encode stable p53 proteins that can detected by standard immunocytochemical techniques unable to detect rapidly degraded wild-type protein. Using of a monoclonal antibody to p53 antigen, immunocytochemical analysis of 29 squamous cell carcinomas of tongue(n= 19) and tonsil(n= 10) was performed. Non-tumor nuclei showed all negative reactivity. Positive reactivity was found in 4/29(13.8%)of SCCs of tongue and tonsil. In sizes of primary tumor, the cases over 4cm showed more positive reactivity than the cases under 4cm(p < 0.05). There was no stastical correlation between the reactivity and histopathologic grades, the primary sites of tumor or the presence of cervical metastasis.

• Proceedings of the KOR-BRONCHOESO Conference
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• 1993.05a
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• pp.88-88
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• 1993

Immunohistochemical staining for keratin proteins may be useful as a diagnostic parameter in head and neck neoplasm. Our study evaluates the keratin antibody staining properties of normal tissues and squamous cell carcinoma of the vocal folds from surgical procedures performed on 27 cases. In normal epithelia, low molecular weight cytokeratins were strongly positive in basal layer but apparently reduced in suprabasal layers and completely negative in superficial layer. In invasive squamous cell carcinomas, low molecular weight anti-ck Ab were positive in all carcinoma cells of poorly differentiated carcinomas. On the other hand, high molecular weight anti-ck Ab were positive in almost carcinoma cells of well differentiated carcinomas.

• Journal of Gastric Cancer
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• v.7 no.3
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• pp.160-166
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• 2007

Purpose: Recently, chemosensitivity tests have become widely used for the selection of effective drugs in gastric cancer patients. In this study, a chemosensitivity test was performed to select agents to increase the effectiveness of adjuvant chemotherapy. Materials and Methods: Chemosensitivity testing was performed in 81 gastric cancer patients that received a gastrectomy at the Yeungnam University Hospital. An ATP (adenosine triphosphate) based chemotherapy response assay was used. Clinicopatholgical factors such as sex, age, expression of tumor markers (CEA and CA19-9 levels), location of the tumor, morphology of advanced cancer, histological type, cell differentiation, depth of invasion, Lauren classification, Ming classification, lymphatic invasion, vascular invasion, neural invasion, lymph node metastasis and TNM stage were used to correlate the chemosensitivity and clinicopathological factors. Results: The most effective antitumor agents in gastric cancer patients were (in order of effectiveness) 5-FU, Epirubicin, lrinotecan and Oxaliplatin in our series. The chemosensitivity test showed a significant difference in susceptibility according to clinicopathological factors. Conclusion: Further studies on multidrug therapy are needed to evaluate synergistic effects of drugs. Therefore, for effective chemotherapy, it is more efficacious to select a chemosensitive drug than continue to use the same drug regimen.