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The Structure of Digestive Tract and Histological Features of the Larvae in Sevenband Grouper, Epinephelus septemfasciatus  

Park, Jong Youn (Aquaculture Management Division, Aquaculture Research Institute, NIFS)
Kim, Na Ri (Ulju-gun Metropolitan City Livestock and Marine Industry Division)
Park, Jae Min (Gyeongsangbuk-Do Native Fish Business Center)
Myeong, Jeong In (Aquaculture Management Division, Aquaculture Research Institute, NIFS)
Cho, Jae Kwon (Aquaculture Management Division, Aquaculture Research Institute, NIFS)
Publication Information
Korean Journal of Ichthyology / v.28, no.1, 2016 , pp. 9-18 More about this Journal
Abstract
Histological and morphological development of the digestive tract of sevenband grouper were observed from after hatching to 60 days. Fishes were fed with rotifer (Brachionus rotundiformis) and chlorella (Chlorella ellipsoidea) after hatching from 2 to 20 days rotifer and brine shrimp (Artemia salina) in after 20 days rotifer, brine shrimp and semi-dry artificial diet in after 23 days. Histological and morphological development of ten larvae was observed by paraffin embedding method after fixing in 10% neutral buffered formalin. Sevenband grouper RLG showed characteristics of carnivorous fish by average 0.87. Larvae after hatching can't open the mouth and anus digestive tract was observed in a straight line following yolk sac. Larvae was observed feeding activity by opening the mouth and anus. Metamorphosis started 8 days after hatching. Esophagus divided four layer, and goblet cell was observed in esophagus, mid intestine and rectum. Larvae started cannibalism and it was caused by difference of growth. The inside of stomach was differentiated to cardiac orifice, body of stomach, pyloric stomach, and pyloric caeca. Goblet cell was observed all intestine. Gastric gland differentiated after hatching 28 days in stomach. Secretion of gastric juice was found at stomach and mucosal fold pyloric caeca. Even thought the inside of stomach expended and the number of gastric gland increased consistently and goblet cell in intestine and mucosa became longer, histochemical changes follow couldn't be found during transforming juveniles 38 days after hatching.
Keywords
Digestive; Epinephelus septemfasciatus; juvenile; larvae; sevenband grouper;
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