• Journal of the Korea Organic Resources Recycling Association
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• v.8 no.2
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• pp.71-76
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• 2000

The biological carbon dioxide fixation using microalgae has been known as an effective carbon dioxide reduction technology. With many environmental factors influencing microalgal productivity, the desirable cultivation factors were investigated using a green alga, Euglena gracilis. It has the high protein and vitamin E to be used as fodder. In batch culture with a photobioreactor, initial pH, temperature, carbon dioxide concentration and light intensity in the optimum cultivation condition were 3.5, $27^{\circ}C$,5-10% and $520{\mu}mol/m^2/s$, respectively. After that, the optimum hydraulic retention time (HRT for the continuous cultivation was 4 days at carbon dioxide concentration of 10%. In this condition, the final dry cell weight was 1.2g/l.

• Proceedings of the Korea Water Resources Association Conference
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• 2019.05a
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• pp.265-265
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• 2019

기후변화로 의한 기온의 상승은 가뭄, 홍수와 같은 재해를 일으킬 뿐만 아니라 깊은 호수나 저수지와 같은 수자원에도 용존 산소, 물질, 영양소 및 식물플랑크톤의 수직적 분포 등과 같은 다양한 부분에 영향을 미친다. 본 연구의 목적은 SWAT, HEC-ResSim 및 CE-QUAL-W2(이하 W2)모델을 사용하여 미래의 기후 변화에 따른 소양호의 수온, 성층강도 및 열적 안정성의 변화를 장기 예측하고 그 영향을 평가하는데 있다. W2 모델의 보정은 2005 년부터 2015 년까지의 실측 과거 데이터를 이용하여 보정하였고 기후변화 시나리오는 IPCC의 AR5 RCP 4.5 시나리오를 사용하였다. 기후자료는 GCM 모델인 HadGEM2-AO 결과를 상세화하여 모의기간의 자료를 생성하였다. SWAT모델을 이용하여 모의기간인 2016 년부터 2070 년까지 일단위로 저수지 유입을 예측했으며 HEC-ResSim모델을 이용하여 소양강댐 저수지 운영 조건에 따라 저수지 방류량 및 수위 변화를 모의하였다. 수온 해석을 위해 W2를 적용하여 저수지의 장기간의 수온 변화를 예측하였다. 결과적으로 대기 온도는 $0.0279^{\circ}C/year$(p < 0.05) 상승할 것으로 예측되었으며, 동일기간 상층(수면으로부터 5m 깊이)과 하층 (바닥으로부터 5m 높이) 수온은 각각 $0.0191^{\circ}C$/년(p < 0.05) 및 $0.008^{\circ}C$/년(p < 0.05) 상승할 것으로 예측되었다. 모의된 수온을 계절별로 분석했을 때 상층수온은 여름철 가장 큰 폭으로 상승하였으며 하층의 경우 겨울철에 가장 큰 폭으로 상승하였다. 계절별 상-하층 수온의 차는 여름이 가장 컸으며, 겨울에 온도차가 가장 작았다. 또한 미래 온도의 상승에 따라, 소양호의 성층 강도가 강해지는 경향을 보였으며 상층 및 하층의 온도차 $5^{\circ}C$를 기준으로 성층이 형성되는 기간은 큰 변동이 없었으나 소멸되는 시점이 점점 늦어지는 추세를 보여 성층 형성 기간이 길어지는 것으로 나타났다. 저수지 표면의 수온 상승은 식물플랑크톤의 계절 성장률에 영향을 미쳤는데, 특정 조건에서 규조류는 최적 성장 범위를 벗어나는 고온 조건에서 성장속도가 감소하였으나 녹조류와 남조류의 출현 시기가 빨라지며 장기화될 것으로 예측되었다.

• Bulletin of Food Technology
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• v.9 no.1
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• pp.127-135
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• 1996

카로티노이드는 동식물계에 널리 분포하고 있는 황, 등, 적 혹은 자색의 대표적인 천연색소군으로서 자연계에서 약 1억톤/년 생산되고 있다. 이와 같이 막대한 량의 카로틴의 저장고는 식품의 잎, 동물플랑크톤, 조류 등이며 따라서 카로틴의 산업적 이용은 대단히 중요하다. 카로티노이드의 종류는 현재 약 600여종 정도 밝혀져 있으며 세계적으로 널리 이용되고 있는 것은 천연과 합성품을 포함하여 $\beta$-카로틴, $\beta$-아포카로테날, 칸타잔신, 아스타잔신 등과 그외 아나토추출물, 인삼유, 옥수수유, 파프리카, 사프란, 토마토추출물, 조류(algae) 등을 들 수 있으며 이들 카로티노이드 소재는 의약품, 식품, 사료등 다양한 용도로 사용되고 있다. 한편 $\beta$-카로틴, 아스타잔신 및 일부 카로티노이드 등은 30년간의 역학적 조사와 최근의 실증실험을 통해 암발생을 억제하는 물질로 규명되고 있으며 강력한 항산화성을 나타내고 있을 뿐만 아니라 동물의 번식 및 성장률 개선, 질병발생 억제, 어육의 색상개선 등 고차기능성을 나타내는 것으로 알려지고 있어 향후 다양한 분야에 이용가능성이 높아지고 있다. 본고에서는 최근 주목을 받고 있는 카로티노이드를 중심으로 생물 기능성 및 향후 이용전망에 대해 다루고자 하였다.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.4 no.2
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• pp.136-143
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• 1999

To understand the growth of attached microalgae to the immersed artificial surfaces in seawater with exposure time, chlorophyll a (chl a) concentration and abundance of attached microalgae to glass slides, and primary productivity and chl a concentration on coverglasses were investigated in Incheon Harbour in May, June 1996 and January-February 1997. Chl a concentrations of microalgae and abundances of diatoms attached to glass slides reached 62.5 mg chl a $m^{-2}$ and $144{\times}10^3$ cells $cm^{-2}$, respectively, during the study period. Chl a concentrations increased with exposure time, and they were significantly correlated with the abundances of attached diatoms ($r^2=0.79$, p<0.001). The chl a concentrations of attached micro algae on coverglass reached the maximum values of 31.1 mg chl a $m^{-2}$ and 65.4 mg chl a $m^{-2}$, and then decreased in May, June 1996. But in January-February 1997, the chl a concentration increased continuously up to 98.9 mg chl a $m^{-2}$. The primary productivity reached the maximum values of 63.1 mgC $m^{-2}\;h^{-1}$, 347.0 mgC $m^{-2}\;h^{-1}$ and 78.3 mgC $m^{-2}\;h^{-1}$, respectively, in May, June and January-February. The primary productivity in May and June varied in accordance with chl a concentrations. But in January-February, the primary productivity decreased from 26 days of exposure while chl a concentration continued to increase. Two cases that primary productivity decreased abruptly seemed to be caused by decrement of chl a and light specific $P^B$ (chl a specific primary productivity) (May and June) and by decrement of light specific $P^B$ due to photoinhibition (January-February). The results of present study indicated that chl a concentrations and the primary productivity of microalgae attached to artifical surfaces immersed in seawater would expedite analysis of dynamics of biomass and physiological status of attached microalgae during biofilm formations.

• Applied Chemistry for Engineering
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• v.28 no.3
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• pp.306-312
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• 2017

Changes in the cell growth and lipid accumulation of marine microalga Dunaliella tertiolecta were investigated in response to the combination of different stress factors including the variation of iron supply as a primary stress factor and different options in light irradiation and $CO_2$ supply as a secondary stress factor. High or limited Fe conditions could act as a stress for lipid synthesis. As a secondary stress factor, non-$CO_2$ condition was good for lipid accumulation, but the overall cell growth was sacrificed significantly after a long-time cultivation. Dark condition as a secondary stress factor also favored lipid accumulation and the extent of cell density reduction at the early period in the dark was small compared to other stress conditions. The two-stage cultivation strategy was necessary to maximize lipid production because tendencies of the cell growth and lipid content were not identical under the chosen stress condition. The first stage was for preparing a high cell density under the normal growth-favoring condition and the second stage was the stress condition to induce lipid accumulation in a short time. The short-term (12 h) incubation under the 5X Fe (3.25 mg/L) and dark conditions resulted in the best lipid productivity of 1.44 g/L/d providing 2 g/L inoculum at the second stage.

• Korean Journal of Ecology and Environment
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• v.40 no.2
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• pp.234-243
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• 2007

Algal growth potential (AGP) test was performed to evaluate the water quality and changes in phytoplankton communities before and after a heavy rain event at six sampling sites in Cheonggye Stream (St. 1 and 2), Jungnang Stream (St. 3 and 4), and Lower part of Han River System (St. 5 and 6) after rehabilitation of Cheonggye Stream, October 2005. To test AGP on each sampling site, cyanobacterium Microcystis aeruginosa was applied as a standard alga. Total nitrogen (TN) showed high values at Jungnang Stream, while St. 4 recorded highest values in this study. However, TN values of Cheonggye Stream and Lower Part of Han River showed similar levels. Total phosphate (TP) also showed high values at Jungnang Stream, while St. 4 recorded highest. However, TP in Cheonggye Stream were extremely low levels. Although chlorophyll-${\alpha}$ (chi-${\alpha}$) contents before the rain event were similar through the sampling sites, chl-${\alpha}$ after the rain increased dramatically at Jungnang Stream and Lower part of Ban River. In particular, after the rain, TP was the highest at St. 4, where Cyclotella sp. dominated the phytoplankton community. When compared with control, AGP values before the rain were comparatively low in all sites, while those after the rain highly increased with the dose-dependently of field water added, due perhaps to the increased nutrients by rainfall. Similar results were observed in Cheonggye Stream. Therefore, for the aesthetic fostering for the citizens, although Cheonggye Stream was Presently being sustained by treated water supply, they have a potential of outbreak of phytoplankton by the increased nutrients supply when a heavy rain comes.

• Korean Journal of Environment and Ecology
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• v.30 no.1
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• pp.92-97
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• 2016

The purpose of this study was to improve growth and survival rate of marine microalgae and macroalga zoospores using with eco-friendly capsulation materials. The capsulation materials were chosen an alginic acid which extracted from marine brown algae combining with starch and calcium chloride. The capsulated microalgae, Nannochloropsis salina and macroalga zoospores, Ulva australis were evaluated with growth and survival rate. When the mixed ratio of alginic acid was less than 50%, capsule formation was not performed. When the ratio of 50% alginic acid and 50% starch, the microalgae was shown the highest growth and survival rate increasing up to $8.74{\times}10^5cells\;mL^{-1}$ while 100% of alginic acid was the lowest rate up to $4.92{\times}10^5cells\;mL^{-1}$. The increasing starch ratio improved to their growth and survival rate, however decreasing alginic acid make physical capsule formation weaken. By applying on a surface of artificial reef, capsulated algal zoospores were germinated 99 individuals $cm^{-2}$. This attempt will be provided to basic core technology for marine afforestation in coastal area.

• Korean Journal of Environmental Biology
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• v.38 no.1
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• pp.114-126
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• 2020

Bloom-forming toxic cyanobacteria Microcystis spp. are common in the summer season in temperate freshwater ecosystems. Often, it leads to the degradation of water quality and affects the quality of drinking water. In a previous study, NQ (naphthoquinone) compounds were shown to be effective, selective, and ecologically safe algicides for Microcystis spp. blooms. To analyze the superiority of developed NQ derivatives, we conducted a microcosm experiment using clay, which is frequently used in South Korea. Similar to previous studies, the NQ 40 and NQ 2-0 compounds showed high algicidal activities of 99.9% and 99.6%, respectively, on Microcystis spp. at low concentrations (≥1 μM) and enhanced phytoplankton species diversity. However, when treated with clay, a temporary algicidal effect was seen at the beginning of the experiment that gradually increased at the end. In addition, treatment with the NQ compounds did not affect either the abiotic or biological factors, and similar trends were observed with the control. These results showed that the NQ 2-0 compound was more effective, with no ecosystem disturbance, and more economical than the currently used clay. These results suggest that NQ 2-0 compound could be a selective, economically and ecologically safe algicide to mitigate harmful cyanobacterial blooms in the field.

• Korean Journal of Microbiology
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• v.50 no.4
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• pp.313-318
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• 2014

The world is in need of sustainable and eco-friendly energy sources such as microalgal biodiesel due to global warming and fossil fuel shortages. In this study, we compared the effectiveness of liquid fertilizer produced from swine manure and agriculture grade solid fertilizers as nutrient sources for microalgal biomass production. Mixed culture (Chlorella spp., Scenedesmus spp., Stigeoclonium spp.; CSS) was cultivated for 28 days in Small Scale Raceway Pond (SSRP) using various nutrient sources (swine manure liquid fertilizer, agricultural solid fertilizer, and mixture of these two fertilizers). Biomass and lipid productivity of fertilizer mixture were the highest at 0.8 g/L and 5.8 mg/L/day, respectively. These results indicate that the fertilizer mixture can provide microalgae necessary nutrient sources for stable biodiesel production and biomass growth. In addition, overall cost of microalgal cultivation and subsequently biodiesel production would be significantly reduced.

• 한국해양학회지
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• v.22 no.4
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• pp.257-270
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• 1987

Fouling communities developing in Jinhae Harbor and Masan Bay were studied by slide and panel immersion test during the period from Dec., 1982 to Nov., 1983. The total viable count of bacteria was estimated more than 1.7${\times}$ 10$\^$4/CFU/$\textrm{cm}^2$ after 15 days of immersion and 46 taxa of benthic diatoms were classified in micro-community. Progressional change of fouling communities was clearly shown and dominant diatom species are Licmophora flavellata, Navicula grevillei, and Nitzschia closterium Major macrofouling organisms are Mytilus edulis, Balanus amphitrite amphitrite, Hydroides ezoensis, and Celleporina sp. Wet weight production of macrofouling organisms exceeds 500g/100cm$\^$2/ after 5months of immersion. Regional defferences in community development are clearly shown in two study areas, and mainly due to the disparities of physicochemical stability and nutritional status of ambient water. Seasonality of larvae and the growth rate are the important factors in fouling community development. Overall process of community development is as follow : bacteria and diatoms-multicellular algae-barnacle, mussels and polychaete-sponge, anemone and ascidian.