• The Korean Journal of Nuclear Medicine
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• v.36 no.2
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• pp.133-139
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• 2002

Purpose: Scintillation Proximity Assay (SPA) does not require the physical separation of receptor bound form from free form. SPA was applied to the study of interaction of human chorionic gonadotropin (hCG) and $anti-{\alpha}$ hCG in serum. Materials and methods: $Anti-{\alpha}$ hCG was biotinylated for the binding to streptavidin. The assay was based on the simple competitive binding method between $[^{125}I]hCG$ and the hCG in sample serum, with $anti-{\alpha}$ hCG-coated beads. Aliquots of biotinylated $anti-{\alpha}$ hCG were dispensed into scintillation vials containing $100{\mu}{\ell}\;[^125}I]hCG\;and\;200{\mu}{\ell}$ of either a standard concentration of hCG for preparation of standard curve or unknown sample, and incubated for 20 min. at room temperature. Then $20{\mu}{\ell}$ streptavidin-coated beads were added to vials, and finally incubated for 10 min at room temperature. Values for unknown samples were then calculated from the standard curve. Results: Optimal background counts were certificated using varied radioactivity of radionuclides. Appropriate standard curve was obtained from SPA method successively, and the concentration of hCG from unknown serum was determined by standard curve. The result from SPA assay was similar to that of RIA. Conclusion: This observation confirms that SPA method could be useful for clinical diagnosis.

• The Korean Journal of Pharmacology
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• v.28 no.1
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• pp.1-9
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• 1992

Catecholamines, serotonin and their metabolites were measured in the posterior hypothalamus of urethane-anesthetized normotensive Wistar Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) using brain microdialysis which is a recently developed experimental method to measure the release of neurotransmitters and their metabolites at the localized brain area in vivo. Microdialysis probe was implanted stereotaxically to the rat posterior hypothalamus and perfused by Ringer's solution. Monoamines and their metabolites were quantified by reverse phase high performance liquid chromatography with electrochemical detection. In vitro recovery test of microdialysis showed that there exist inverse relationship between the perfusion flow rate and the relative recovery of neurochemical compounds. The estimated extracellular concentration of dopamine was about 32 nM, of norepinephrine 50 nM, of epinephrine 50 nM, of serotonin 73 nM, of 3, 4-dihydroxyphenylacetic acid (DOPAC) 281 nM, of homovanillic acid (HVA) 181 nM, and of 5-hydroxyindoleacetic acid (5HIAA) 3767 nM in the hypothalamic perfusate of the normotensive rat. There was no difference in the basal level of monoamines between the SHR and the WKY. In contrast, the level of DOPAC, HVA and 5HIAA in SHR was higher than that in the WKY, This study demonstrated that the microdialysis technique should be an applicable tool for in vivo measurement of central neurochemical substances.

• Journal of Digital Convergence
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• v.11 no.11
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• pp.661-666
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• 2013

The aim of this study was to evaluate the property of heterophoria and fusional reserve in Adults in Jeon-buk Area. We examined the corrected visual acuity, corrected refractive error, heterophoria and fusional reserve of 116 healthy myopes aged from 20 to 44 old who had no strabismus no ocular and phyisical diseases. Using Von Graefe test of horizontal heterophoria Measurement, we measured orthophoria(26.7%), exophoria(52.5%) and esophoria(20.7%) for at near distance. The subjects who had exophoria of 0-6${\Delta}$ in the range of normal state was 38.8%, while the subjects who had exophoria in the range of abnormal state was 61.2%. Reducing fusional reserve was associated with increasing phoria. We found a relationship between asthenopia and fusional reserve of heterophoria and considered that fusional reserve must be examined when we preserve for a patient with heterophoria. Furthermore, Gradient method AC/A ratio was found 4.03 and its relationship to refractive error could not be determined.

• Journal of Oral Medicine and Pain
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• v.26 no.3
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• pp.277-290
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• 2001

영상촬영술은 개방형 외과적 관절수술이나 관절경 검사법을 할 수 없는 상태에서 관절의 상태에 대한 시각적 정보를 확보할 수 있는 유일한 방법이다. 이것의 주된 목적은 진단과 치료계획과정을 도와주는 정보를 제공하는데 있다. 그중 방사선 촬영술은 턱관절의 구조적 질병을 진단하는데 기본적인 수단으로서 오래동안 사용되어 왔 다. 그러나 어떠한 방사선 소견이 개별 관절질환의 특징적 소견인지는 찾아내기가 어려운 실정이다. 그러나 통상적 턱관절 방사선 촬영술로서도 구조적 골변화를 찾아낼 수 있으며 특히 시상 단층촬영술은 턱관절에서 가장 유익한 정보를 보여준다고 한다. 또한 보고에 의하면 턱관절 장애는 다양한 해부학적 요인들과 관련이 있다고 한다. 따라서 본 연구에서는 통상적인 방사선 단층촬영술을 이용하여 턱관절 장애환자의 턱관절에서 나타나는 골변화를 찾아내고 이러한 골변화가 하악과두 수평각, 하악과두 형태, 과두위치 등과 같은 여러 요인들과 서로 관련이 있는 지 찾아보고저 하였다. 단국치대 구강내과 안면동통진료실에 내원한 256명의 환자 중, 턱관절장애를 편측으로만 호소하는 환자 73명을 대상으로 SCANORA를 이용하여 방사선 단층촬영을 시행하였다. 먼저 악하두정위 촬영을 통해 정중선에 대한 하악과두의 방향을 찾아내고 단층촬영 부위를 계산하였으며, 모든 촬영면은 4 mm 두께로 하고 턱관절 부위에만 국한되도록 조준하였다. 폐구 시 4개의 시상 촬영과 개구 시 한개의 시상 및 전두촬영상을 구한 후, 하악과두, 과두 형태 및 하악과두위치 등과 같은 요인들에 대한 골변화간의 관련성을 조사하고저 자료를 측정한 후 Contengency table analyses를 시행하였다. 본 연구의 결과에 따르면 결론적으로 하악과두의 형태, 하악과두의 수평각 및 하악과두의 위치 등은 턱관절의 골변화와 상호 관련이 있다는 가설을 확인할 수 있었다. 즉 하악과두상의 골변화는 과두가 후방위치되고 과두각이 25도 이상 크며, 특히 20대에서는 flat type, 40대에서는 angled type의 과두형태를 가지며 두 과두각의 차이가 9도에서 12도 정도로 큰 차이가 있는 남성환자에서 증가한다.

• Journal of Korean Ophthalmic Optics Society
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• v.5 no.1
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• pp.139-146
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• 2000

Once refractive error of the eye was fully corrected, Maddox rod was used to investigate binocular vision function. When the deviations of subjects' eyes were measured, orthophoria. without any horizontal deviations, was found in 10%, esophoria in 55% and exophoria in 34% of the patients. Esodeviation of the eye was found to be greater than exo-deviation by 11%. This is also refracted in deviations found to be greater than $4{\Delta}$ diopter magnitude where esophoria was 23% and exophoria was 9%. Thus for the patients to achieve comfortable binocular vision function, binocular vision anomalies need to be considered in spectacle prescription. In examining ocular anomalies of corrected refractive error, the deviation of the eye differed from under corrected refractive power to over corrected refractive power. There was a decrease in exophoria and an increase in esophoria, with over correction. This was thought to be due to accommodation. Furthermore, calculated AC/A ratio was found to vary from 1.25 to 6.61 and its relationship to refractive error could not be determined.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1993.04a
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• pp.156-156
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• 1993

본 연구에서는 생체 뇌 국소부위의 세포외액을 대상으로 실험할 수 있는 뇌 미세투석법을 이용하여 후시상하부에서 유리되는 monoamine의 세포외액 농도에 대한 전신 혈압변화의 영향을 관찰함으로써, 심혈관 조절에 대한 후시상하부의 monoamine성 뉴론의 생리적 역할을 규명하고자 하였다. 마취한 흰쥐의 머리를 뇌정위 고정장치에 고정시키고 미세투석관 (microdialysis probe)을 후시상하부에 위치시킨 후 링거액으로 관류하였다. 미세투석액내에 존재하는 monoamine성 신경 전달물질들과 그 대사체들의 정량분석은 고속액체크로마토그라피와 전기화학검출기를 이용하여 실시하였다. 전신 혈압을 40분 동안 약 40mmHg 상승 혹은 30mmHg 감소시키기 위하여 L-phenylephrine hydrochloride (800ng/100 g/min) 혹은 nitroprusside dihydrate (500 ng/100 g/min)를 대퇴정맥을 통하여 주사하였다. 후시상하부로 부터 20분 간격으로 얻은 투석액에서 신정화학물질들의 농도는 미세투석관 삽입후 2시간에 안정되었다. 관류액의 $K^{＋}$ 농도를 90 mM로 증가시켰을 때 후시상하부의 투석액에서 norepinephrine (NE) 과 serotonin (5-HT)의 농도는 각각 기준치의 176.5 $\pm$ 14.8%, 149.1 $\pm$ 2.3%로 증가하였다. Phenylephrine (i.v.)으로 유발된 전신 혈압상승에 의하여 NE과 5-HT의 양은 각각 기준치의 79.3 $\pm$ 4.4%, 61.4 $\pm$ 10.3%로 유의하게 감소하였다. 또한 nitroprusside (i.v.)에 의하여 전신혈압이 감소하였을 때 투석액내 5-HT의 양은 기준치의 195.0 $\pm$ 23.0% 로 유의하게 증가하였다. 후시상하부의 투석액내 monoamine성 대사체들의 경우에는 전신 혈압의 변화에 대한 유의한 반응을 나타내지 않았다. 이상을 종합하면 전신 혈압이 증가되었을 때는 말초 압수용체의 흥분에서 기시한 신경충동이 후시상하부에 전달되어 신경말단에서 5-HT 과 NE 의 유리가 감소되고, 5-HT의 경우에는 전신 혈압이 감소되었을때 그 반대 현상이 일어난다는 가설을 제시할 수 있다. 본 연구의 결과는 생체상태에서 혈압변화에 대한 후시상하부의 monoamine성 조절양상을 규명한 것으로서 특히 후시상하부의 serotonin성 신경계를 통한 심혈관 중추조절의 가능성을 처음으로 제시한 것이다.

• Journal of Navigation and Port Research
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• v.30 no.8 s.114
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• pp.685-690
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• 2006

The rail clamp is the device to prevent that a crane slips along rails due to the wind blast as well as to locate the crane in the set position for loading and unloading containers. The wedge typed rail clamp should be designed to consider the structural stability and the durability because it compresses both rail side with large clamping force by the wedge working as the wind speed increases. In this research, the design of experiments(DOE) and the variation technology(VT) built in ANSYS WORKBENCH are utilized to determine the optimum shape of a jaw. The optimum results obtained by two methods are compared and examined.

• Progress in Medical Physics
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• v.7 no.1
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• pp.37-52
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• 1996

Image-guided localized, water-suppressed in vivo $^1$H MR spectroscopic studies were performed on the patients with brain tumors, acute cerebral infarction and schizophrenia, and dogs. GE Signa 1.5 T whole-body MRI/MRS system using STEAM pulse sequence was used. Proton metabolite ratios relative to creatine (Cr) were obtained using a Marquart algorithm. In vivo $^1$H MR spectra in brain neoplastic tissues revealed the changes of signal intensities of N-acetylaspartate (NAA), choline (Cho) and lactate (Lac) resonances. The present results suggest that the observed metabolite alterations from localized, water-suppressed in vivo $^1$H MR spectroscopy can be useful as an index of brain tumors, cerebral infarction and schizophrenia, and provide good quality metabolic information of cerebral tissue in the field of thanato-chronology.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2006.06b
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• pp.331-336
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• 2006

The rail clamp is the device to prevent that a crane slips along rails due to the wind blast as well as to locate the crane in the set position for loading and unloading containers. The wedge type rail clamp should be designed to consider the structural instability and the durability because it compresses both rail side with large clamping force by the wedge working as the wind speed increases. In this research, there are two methods which are design of experiment and variation technology in used commercial software and shape optimization was performed. The optimum results obtained by the two methods are compared and examined.

• Journal of Chest Surgery
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• v.30 no.6
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• pp.566-572
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• 1997

A major obstacles to evaluation of newly-developed treatment strategy for human lung cancer has been the lack of appropriate experimental animal models. We describe a new experimental model of orthotopically-developed non-small cell lung cancer in nude rat, involving inoculation of tumor cell suspension by thoracotomy. Over 40 direct implantation to the periphery of the lung has been performed to date, each requiring less than'1 hour for completion. This model has been used to perform a series of experiments to investigate whether the rat lung and surrounding structures trapped tumor cells with 2 different non-small cell lung cancer cell lines(NCI-H46O and NCI-H1299). Every animal showed development of tumor masses, which were loculated at the periphery of the lung karenchyma and identified also by radiography. After 3 weetu of the inoculation, tumor develop meat at the mediastinal strutures were identified. The life expectancies of the victims were different between the cell lines, but were approximately 5 weeks when NCI-H46O cell line was used. This new orthotopic lung cancer model may be facilitate future studies of the new therapeutics of localized non-small cell lung cancer .