• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2002.11b
• /
• pp.29-29
• /
• 2002

참오갈피(Acanthopanax pedunculus)는 국내 고유한 자생 종이며 현재 몇 그루 만이 존재하는 것으로 추정된다. 이 수종은 대체적으로 다른 오갈피 수종에 비해 잎 및 열매는 물론 식물 전체 부위가 다소 커서 경제성이 높다고 판단된다. 본 실험에서는 참오갈피의 세포배양 기술을 이용한 묘목 및 약용원료의 산업적 대량 생산 기술을 개발하고자 수행 하였다. 참오갈피 접합자배를 적출하여 무균적으로 배양한 다음 자엽단계로 성숙한 이들 접합자배를 1 mg/1 2,4-D가 첨가된 MS 배지에 배양하여 배형성 캘러스를 유도하였다. 배형성 세포의 유도율은 약 72%에 달했다. 유도된 배형성 캘러스는 2,4-D가 첨가된 같은 고체 및 액체 배지에서 계대배양하여 배형성 캘러스 및 세포를 유지하였다. 배형성 캘러스를 2,4-D가 첨가되지 않은 배지에 옮겨주면 이들 세포로부터 체세포배가 발생되었다. 약 2개월의 기간을 거쳐 자엽단계로 성숙된 체세포 배는 발아되지 않기 때문에 5 mg/1 GA$_3$가 첨가된 1/2 MS 배지에 옮겨 발아 및 식물체로 재생 시켰다. 약 7 cm 크기로 재생된 식물체를 인공토양에 옮겨 1달간 순화시키고 난 후 토양에 옮겼을 경우 87%가 생존하였다. 기내에서 유도된 참오갈피 식물체는 특별한 처리를 하여주지 않아도 뿌리로부터 배발생 세포를 형성 할 수 있었다. 따라서 이들 식물체로부터 배발생세포 유도는 세포주의 보존 및 증식에 효과적으로 이용될 수 있었다. 한편 참오갈피 세포는 5-10 리터 바이오리엑터 배양을 통하여 배형성세포, 자엽단계의 배, 유식물의 대량생산의 가능성을 확인하였다.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.9 no.2
• /
• pp.276-280
• /
• 2008

The design technology to be developed in this study is technology related to the metal panel comer processing method of very high value-added, interior and exterior cladding material, in the architecture. This study is aimed at designing a Bending Die System that enables metal panel comer processing for the first time in Korea, by improving corrosion resistance (durability), weather resistance and elegances (design) for the connecting part of right angle cornering, where most serious problems occur in using metal steel plates of 2.5mm or thicker. This is used as a kind of metal ball and as architectural interior and exterior cladding material.

• Proceedings of the Materials Research Society of Korea Conference
• /
• 2009.05a
• /
• pp.41.2-41.2
• /
• 2009

최근 자동차 시장에서는 레이저 열처리에 대한 관심이 집중되고 있다. 이는 현재까지 사용되어 왔던 점용접에 비해 이음부 형상에 제한이 적고 자유도가 높으며 짧은 시간내에 용접을 마쳐 열변형의 문제가 적기 때문이다. 이러한 이점 때문에 자동차 시장의 기술개발 동향이 차체 부품의 경량화와 원가절감을 위한 신기술 개발로 레이저 가공 기술을 접목시키고 있으며 이러한 신공정 개발은 지속적으로 확대되어 가고 있는 추세이다. 본 실험에서는 차체 접합을 위하여 신기술로 대두 되고 있는 레이저 용접법으로 박판을 접합하였다. 실험 조건은 동일한 재료 두 판을 합쳐 전력량을 변화시켰으며, 전력량의 한 조건을 잡아 용접 속도에 변화를 주었다. 용접 샘플의 물성을 평가하기 위하여 제품 내부의 구조를 비파괴 시험법을 통하여 분석하여 내부적 결함 및 내부 구조를 관찰하고, 열영향부 및 용접부 내부의 조직을 관찰하여 신기술에 대해 재료적 물성을 평가하고자 한다. 평가방법은 산업용 단층 촬영기(CT X-RAY)를 이용하여 재료를 파괴하지 않고 용접상태에 따른 내부구조를 분석하였으며, 용접부, 열영향부 그리고 모재 부분에 대해 경도를 측정하여 용접조건에 따른 경도양상을 관찰하였다. 또한 부위별로 조직을 관찰하여 재료 내부의 상을 관찰하였다.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.9 no.6
• /
• pp.1518-1522
• /
• 2008

The design technology to be developed in this study is technology related to the metal panel corner processing method of very high value-added, interior and exterior cladding material, in the architecture. This study is aimed at designing a Bending Die System that enables metal panel corner processing for the first time in Korea, by improving corrosion resistance (durability), weather resistance and elegances (design) for the connecting part of right angle cornering, where most serious problems occur in using metal steel plates of 2.5mm or thicker. This is used as a kind of metal ball and as architectural interior and exterior cladding material.

• Journal of the Korean Solar Energy Society
• /
• v.34 no.3
• /
• pp.107-114
• /
• 2014

Modular systems are widely used in various building types including housing, dormitory, and barracks. Steel studs have many advantages over other materials as construction components of modular buildings in terms of seismic performance, durability and maintenance. However, steel stud modular systems also have weakness in condensation resistance due to high thermal conductivity of steel. The purpose of this study is to investigate the condensation resistance of steel stud wall corner details in modular buildings by thermal simulation. The condensation resistance was evaluated by temperature difference ratio according to ISO 13788. The result showed that there was little difference between the alternatives of adding cavity and insulation. Separation of interstitial steel studs showed outstanding effect on the improvement of temperature difference ratio.

• Conservation Science in Museum
• /
• v.26
• /
• pp.67-82
• /
• 2021

Two golden beads (Buyeo 5336) housed at the Buyeo National Museum were discovered in 1993 near the site of an ancient workshop in Neungsan-ri in Buyeo-gun, Chungcheongnam-do Province. These rare examples from the Baekje Kingdom of an application of granulation have maintained their original form intact, and thus serve as important materials for the investigation of production techniques applied. This study analyzed the composition of the golden beads using a portable X-ray fluorescence analyzer, a stereo microscope, and a scanning electron microscope with an energy dispersive X-ray spectrometer. The manufacturing technique was examined through the observation of the micro-shape and the surface condition and by a composition analysis of the joint part. In both beads, a hole was pierced in a hollow body and the bead was decorated with golden wires around the hole and gold granules in other parts. In some areas, golden granules had been attached to the gold plate and golden wires were then placed over the granules. The purity of both the wires and the granules was analyzed as 23.6 - 23.7K. A high copper content was detected in some of the parts where the granules were attached. The findings of a previous reproduction experiment and study of production methods suggest that the beads were made using the copper diffusion technique.

• Journal of Korean Society of Steel Construction
• /
• v.17 no.5 s.78
• /
• pp.561-568
• /
• 2005

The object of this paper is to determine appropriateness for use of high-strength tensile bar as a tension web member. The gap K-joint of tensile bar types were compared with gap K-joint of square hollow section (SHS) types. For the same width-to-thickness ratio ($2{\gamma}=33.3$ ), tests were performed on four specimens of the SHS type and eight specimens of the tensile bar type. The comparison of capacity with the experimental results showed a capacity of the SHS type joint to be higher than that of the tensile bartype joint for the same brace-to-chord width ratio. Moreover, the capacity of the SHS type joints increased proportionally to the width ratio ${\beta}$), while tensile bar type joints increased as the tension width ratio (${\beta}2$). In failure mode, SHS-type specimens showed local buckling of the compression brace and plastic failure was observed between the tension brace and chord face, and with the tensile bar type specimens there appeared punching shear failure of the chord face at the toe of the connection plate. It is, therefore, concluded that width-to-thickness ratio should be lower than that of the hollow-section type and the relation between tension and compression width ratio should be considered.

• Fire Science and Engineering
• /
• v.31 no.3
• /
• pp.79-87
• /
• 2017

Sandwich panels which are having the both sides are bonded with a heat insulating material with an iron plate are used as factories, warehouse structures as advantages of convenience in construction at economic efficiency of material cost. However, in a panel structure constructed by continuous joining of sandwich panels, a joint portion where a panel and a panel are connected is generated. The joint part is a part which is easily vulnerable to fire because flames easily flow into the melting and deformation of the iron plate during fire. The flames flowing into the panel induce diffusion of fire by rapid burning, causing damage of human life and property. In this research, we developed a flame spread prevention plate to prevent spreading of sandwich panel. This is an improvement of the workability by the anti-spreading construction method of the existing previous research, it can be applied independently to the connecting part where the panel and the panel are coupled, designed to prevent inflow and spreading of flame did. The actual fire test of the test method of KS F ISO 13784-1 of the sandwich panel specimen was conducted and the burning behavior corresponding to the presence or absence of application of the flame spread prevention plate was grasped at the panel connection part and its effect was measured. Inserting a fire spreading plate into the test result panel connecting part is measured by delaying the flashover, prevention of collapse of the specimen, and temperature rise of the opening, effectively improving the fire safety of the panel structure It was confirmed as a method that can be secured. It is judged that panel structure will contribute to ensuring fire safety by applying the fire spread prevention construction method of various methods ensuring the workability and economy of panel connection vulnerable to fire.

• Pediatric Gastroenterology, Hepatology & Nutrition
• /
• v.12 no.2
• /
• pp.177-182
• /
• 2009

Purpose: Colitis is a condition associated with a spectrum of altered morphologic changes and cellular adhesion. E-cadherin plays a key role in the establishment and maintenance of epithelial tissue structure and cell-cell adhesion. The purpose of this study is to evaluate E-cadherin expression in colonic epithelium of various colitis in children. Methods: The expressions of E-cadherin were examined in 39 cases of colonic mucosal biopsy specimen using immunohistochemical staining. When more than 50 percent of cells exhibited uniformly the same intensity and pattern of immunostaining as the adjacent normal mucosa, the antigen expression was considered normal. Abnormal expression was defined when less than 50 percent of cells stained, when cells showed a heterogeneously weak or altered distribution, or when complete absence of staining was observed. Results: Fifteen cases with non-specific colitis (38.5%), 7 cases of with Crohn's disease (17.9%), 5 cases of infectious colitis and milk protein sensitive proctocolitis (12.8%), 3 cases of ulcerative colitis (7.7%), 2 cases of Henoch-Schonlein purpura colitis (5.1%), one case of Behcet's disease and ischemic colitis (2.6%) were included in this study. E-cadherin expression was decreased in all kinds of colitis. Reduced expression of E-cadherin was observed in 77 percent of cases. E-cadherin was weaker or no expression in reparative epithelium and "ulcer associated cell lineage". Conclusion: Altered expression of E-cadherin occurs during mucosal inflammation in any kinds of colitis. These changes may be involved in promoting cell migration during epithelial restitution of the gastrointestinal mucosa.

• Journal of Plant Biotechnology
• /
• v.29 no.4
• /
• pp.241-246
• /
• 2002

Somatic embryos or adventitious buds were formed from the segments of zygotic embryo of Lycium chinense Mill. on Murashige and Skoog (MS) medium supplemented with auxins (2,4-D, NAA, IAA) and / or cytokinins (zeatin, kinetin, BAP). Embryogenic callus formed on MS medium containing 0.5 mg/L 2,4-D and then differentiated into somatic embryos without transfer to hormone free medium. On the other hand, adventitious buds were formed on medium with 0.01 mg/L 2,4-D and 0.5 mg/L zeatin. Among various parts of zygotic embryos, the morphogenic potential was higher in the cotyledonary region, and the most organogenic potential was found in cotyledon followed by radicle, hypocotyl, and whole embryo. Histologically, bipolar structure of the heart-shaped embryos were confirmed and in adventitious buds only shoot apical meristems were found to exist.