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Complete Genome Analysis of Hyphantria cunea Nucleopolyhedrovirus Isolated in Korea (한국에서 분리한 미국흰불나방 핵다각체병 바이러스의 전장 유전체 분석)

  • Choi, Jae-Bang;Kim, Hyun-Soo;Woo, Soo-Dong
    • Korean Journal of Organic Agriculture
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    • v.31 no.4
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    • pp.395-412
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    • 2023
  • The morphology and whole genome sequence of Hyphantria cunea nucleopolyhedrovirus W1 (HycuNPV-W1) isolated in Korea were analyzed for the use as an eco-friendly control agent against H. cunea. The HycuNPV-W1 had irregular tetrahedral polyhedra with a size of 1.5-2.2 ㎛ which is similar to that of previously reported HycuNPV isolated in Korea. As a result of whole viral genome analysis, HycuNPV-W1 was composed of 131,353 bp, which is 1,606 bp shorter than that of the previously reported HycuNPV. The G+C content was 45% and six of the homologous repeated regions were found, so there was no significant difference from the previous report. As a result of ORF analysis, HycuNPV-W1 contains total of 145 ORFs which is three ORFs less than the previous report, while two ORFs were exclusively found in HycuNPV-W1. The functions of these ORFs remains unclear and are not considered to have a significant influence on the characteristics of the HycuNPV. The genome vista analysis showed that the overall sequence identity between HycuNPV-W1 and the previously reported HycuNPV was very high. The whole genome of HycuNPV-W1 analyzed was found to be similar to those of the previously reported HycuNPV, however, it is supposed to be a novel resource in Korea with different isolate.

A Pursuit of Innovation in the Korean Genetics-Genomics Research System through a Culturalist Strategy (문화적 전략을 통한 한국 유전학-유전체학 연구체계의 혁신 모색)

  • Lee, Cheong-Ho
    • Journal of Science and Technology Studies
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    • v.6 no.2 s.12
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    • pp.131-183
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    • 2006
  • The Korean genetics and genomics reveal a firm willingness to participate in and contribute to the production of creative scientific knowledge at a world level at present, though they have short past histories of introduction from the Western counterparts and those of education for the next generations. But the Korean genetics and genomics have been developed in a fragmented and biased manner. By reconfiguring the various research projects of genomics into the Genome Project of Korea, which reflect a worldly trend in life science, but have been established in a scattered fashion in Korea, and incorporating some neglected areas of genetics, such as human genetics and theoretical and population genetics which can be reconstructed in a new way, a genetics-genomics research system can be formulated on the multi-tiered perspective of concept, knowledge, and institution, while the system being a subsystem of the national research system of life science in Korea. Innovation can be pursued in the systematic practice through a culturalist strategy. The culturalist strategy with the practice based on the research system consists of 1) intensification of fundamentalness of genetics and genomics, 2) advancement of communitarianism in geneticist-genomicist community, 3) research on the cultural bio-species along with the promotion of scientific arts and culture, and 4)formation of the Korean science studies of genetics-genomics and the diffusion of the knowledge produced. The first two strategy components are the ones that intends to bring out changes in the structural aspect of the scientist community in Korea. The third is the one that attempts to magnify the interface between the scientist community and the Korean society at large and increase its connectivity between both, while the fourth is the one that has an intentionality toward the Korean society outside of the scientist community. This culturalist strategy is intended to increase the cultural constructivity of the genetics-genomics research system in Korea.

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Feasibility Study of Parallel- Plate Detector Using Dielectric film for 6 MV X-ray (6MV X-선 검출특성 조사를 위하여 유전체 필름을 이용하여 제작한 평행판 검출기의 유용성)

  • 조문준;김용은;이병용;김정기;임상욱;김현수;김기환
    • Progress in Medical Physics
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    • v.15 no.2
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    • pp.105-111
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    • 2004
  • The parallel plate detector with dielectric film for dosimetry was designed to measure detection characteristic of 6 MV X-ray with medical linear accelerator. PTFE film was inserted into FEP films that are made by two one-side metal coated materials for ion source. The thicknesses of PTFE dielectric film was 100 ${\mu}{\textrm}{m}$ and the thickness of FEP dielectric film was 100 ${\mu}{\textrm}{m}$, respectively. This detector was fixed by two acrylic plate for physical hardness ad geometrical consistency. The geometrical condition for measurement with parallel-plate for detector was below; SSD=100 cm and the 5 cm depth between detector and phantom surface The major parameter of detector characteristics such as zero drift current, leakage current, charge response by applied voltage, reproducibility, linearity, TMR measurement, dose rate effect were measured. The zero drift currents are 8.3 pA and leakage currents are 10 pA. The charge response of applied voltage is showing linearity in 414 voltage. The measurement deviation of reproducibility in this detector is within 1% for dose and the linearity of applied dose shows in this detector. The TMR curves in phantom between this parallel plate detector and reference detector are matched within 3% deviation from maximum dose depth to 7.5 cm depth. It is considered that this dosimetric system is satisfactory for the purpose of the constancy check of the 6 MV x-ray from medical linear accelerator.

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Design of Miniaturized Broadband Parasitic Patch Antenna Using Reduced Size Main Patch with U-Shaped Parasitic Patches (폭이 좁아진 주 패치와 U자 형태의 기생 패치를 이용한 소형화된 광대역 기생 패치 안테나 설계)

  • Wi, Sang-Hyuk;Kim, Woo-Tae;Hong, Young-Pyo;Yuk, Jai-Rim;Yook, Jong-Gwan
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.18 no.4 s.119
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    • pp.389-397
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    • 2007
  • This paper proposes miniaturized broadband parasitic patch antenna. The proposed antenna consists of a probe fed reduced size main patch and U-shaped parasitic patches. The parasitic patches are incorporated to the radiating edges of the main patch to miniaturize the antenna size. The broadband impedance matching can be achieved by either E-plane or H-plane electromagnetic coupling between main patch and parasitic elements. The size of radiating elements is $18{\times}17.6\;mm^2$ and the overall dimension of designed antenna with substrate and ground plane is $25{\times}30{\times}4\;mm^3$. The fabricated antenna on a FR4 substrate shows two resonant frequencies(5.12 GHz and 6.08 GHz) with 27.3 %(1.5 GHz) fractional bandwidth at 5.5 GHz center frequency. The calculated and measured radiation patterns are almost similar to conventional patch antenna.

A study on dielectric characteristic of phosphate glass-ceramic for AC-PDP (AC-PDP용 인산염 결정화 유리의 유전적 특성에 관한 연구)

  • Kim, Joon-Hyung;Yon, Seog-Joo
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.17 no.3
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    • pp.102-107
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    • 2007
  • Dielectric layer of phosphate glass for plasma display panel (PDP) device made by silk screen printing on soda-lime glass. For regulate thermal expansion coefficient (CTE) of between substrate glass and dielectric layer use addition of $Al_2O_3$ and $TiO_2$. The crystallization process of glass-ceramics for dielectric layer have been examined by DTA, XRD some of optical, electrical properties of the dielectric layer were evaluated by UV-spectrometer, dilatometer, impedance analyser. The principal crystalline phase was identified as zinc metaphosphate [$Zn(PO_3)_2$] and zinc pyrophosphate [$Zn_2P_2O_7$]. Reflectance and dielectric constance increased with the addition of $TiO_2$ filler, dielectric constant lower the out side reflectance unchanging of the adding of $Al_2O_3$ filler. Besides CTE was at about $62{\times}10^{-7}/^{\circ}C$.

Development of Molecular Markers and Application for Breeding in Chinese Cabbage (배추의 분자 마커 개발 및 육종적 활용)

  • Kim, Ho-Il;Hong, Chang Pyo;Im, Subin;Choi, Su Ryun;Lim, Yong Pyo
    • Horticultural Science & Technology
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    • v.32 no.6
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    • pp.745-752
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    • 2014
  • Chinese cabbage (Brassica rapa L. ssp. pekinensis) is an economically important vegetable crop as a source of the traditional food Kimchi in Korea. Although many varieties exhibiting desirable traits have been developed by the conventional selective breeding approach, breeding related to abiotic or biotic stresses, such as a particular pests or diseases, or tolerance to climatic conditions, is likely to be slow. This could be helped by an efficient method for selection from various, rapidly-evolved genetic resources on the basis of molecular markers. In particular, the Brassica genome sequencing project enables genome-wide discovery of genes or genetic variants associated with agricultural traits. We here discuss the recent progress in the field of Chinese cabbage breeding with regard to the application of molecular markers.

소련 체르노빌 원자력발전소 사고원인과 교훈

  • 강종권
    • Journal of the Korean Society of Safety
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    • v.3 no.1
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    • pp.47-54
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    • 1988
  • 소련의 체르노빌 원자력 발전소에서 1986년 4월26일 발생된 전리방 사선누설 사고는 원자력 개발사상 미증유의 것이었다. 이 사고로 많은 사상자를 냈을뿐 아니라 향후 수 10년간 계속해서 막대한 인명피해가 일어날 것이라고 예견한 소련의 미생물학자가 있는가 하면, 서독의 한 과학자는 서독에서만도 4천명에서 2만3천명의 암환자가 새로 발생하고 9만명의 유전장해자가 생길 것으로 염려하고 있을 정도로 그영향은 지구 북반구 전체에 미치게될 것으로 예상되고 있다. 체르노빌 발전소는 소련 우크라이나 공화국의 키에프시 북동쪽 130km에 위치하고 있으며 그 주변 30km안에 약13만명의 주민이 살고 있었다.

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The Bacillus subtilis Genome Sequencing Project in Korea: Sequence Analysis of the 53 kb DNA Fragment at 180$^{\circ}$-185$^{\circ}$- of B. subtilis 168 Chromosome (한국에서의 고초균 유전체 연구: Bacillus subtilis 염색체상 180$^{\circ}$-185$^{\circ}$-부위 53 kb DNA 단편의 염기서열 분석)

  • 김사열;최수근;정영미;신병식;박승환
    • Microbiology and Biotechnology Letters
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    • v.26 no.1
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    • pp.23-33
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    • 1998
  • The entire sequence of a 4,214,810 bp genome of the Bacillus subtilis 168 has been determined by an international project, and the completion has been announced on July 19, 1997. For the sequencing project an international consortium was established and 25 European, 7 Japanese laboratories, 2 biotechnology companies, and our laboratory participated in the project. Within this framework we determined the complete nucleotide sequence of a 53,289 bp fragment upstream of the odhA gene (181 $^{\circ}$) of the B. subtilis 168 chromosome. On the basis of the published DNA sequences of the B. subtilis sspC and odhA genes, we obtained genomic fragments by plasmid rescue and long-range PCR. The sequenced fragment contains 56 putative open reading frames (designated yojA-yolI and 9 known genes (sspC, cge cluster, orfE5, orfRMl and odhA), in which we found many interesting features. In addition, the entire nucleotide sequence of a 53,289 bp region enabled us to revise the current genetic map of this region.

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Temperature Dependence of the Electric-field-induced Strains in PMN-based Relaxor Ferroelectrics (PMN계 완화형 강유전체에서 전왜특성의 온도 의존성)

  • Park, Jae-Hwan;Hong, Guk-Seon;Park, Sun-Ja
    • Korean Journal of Materials Research
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    • v.6 no.4
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    • pp.349-356
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    • 1996
  • 완화형 강유전체의 가장 대표적인 PMN계에서 첨가제의 종류와 함량, 측정온도, 인가 전계의형태 등의 변화에 따른 전계인가 변화특성을 광법위하게 조사하였다. Columbite precursor법에의해 분말을 준비하고 고상소결방법에 의하여 모든 시편을 제조하였다. 순수한 PMN에 첨가제로서 PbTiO3와 Pb(Zr, Ti)O3를 첨가한 경우에 완전한 perovskite 구조의 고용체가 형성되었음을 알 수 있었다. T$\varepsilon$max이상에서는 변위의 이력이 크게 발생하는 강유전체의 거동을 보여주었다. 양방향으로 전계를 인가하여 변위를 이용하면 발생 strain은 실온 근방에서 온도에 대하여 안정적이지만 단방향 전계에 따른 변위는 온도에 따라 크기가 변한다는 것을 알 수 있었고 유전상수가 큰 경우가 전왜의 크기 또한 큰 것을 알 수 있었다. 0.9MN-0.1PT와 0.8PMN-0.2PZT의 경우 최대가 되는 온도는 유전율이 최대가 되는 온도보다 더 낮은 온도에서 나타났다.

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A Permittivity Measurement of Dielectric Slabs Using a Parallel Plate Waveguide (평행판 도파관을 이용한 유전율 측정 방법)

  • Cho, Gyo-Yeong;Park, Wee-Sang
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.12 no.2
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    • pp.199-203
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    • 2012
  • This paper introduces a simple new procedure approach to determine the permittivity of dielectric slabs. The method uses a parallel plate waveguide which supports a TEM mode. The presence of the dielectric slab placed at the bottom of the waveguide makes the speed of the TEM wave slower. The relationship between the change of the speed and the permittivity of the dielectric slab allows the determination of the permittivity. The relationship is analyzed electromagnetically, and the results of measurements are in good agreement with the analysis.