• Applied Microscopy
• /
• v.28 no.2
• /
• pp.215-224
• /
• 1998

To investigate ultrastructural changes on the adrenal glands of the developmental rats, tissues were collected at 20 days of gestation, at birth, 7 days, 15 days and 30 days after birth and studied by electron microscope. In the medulla of adrenal gland of the rat, two types of granules were observed at ultrastructural level. The one were adrenaline $(123\sim200nm)$ and the others were noradrenaline ($177\sim279nm$ of long axis, $124\sim194$ of short axis). Adrenaline granules were characterized by presence of round or spherical with low or moderate electron density. The granules showed a wide lucent or clean halo between the contents and the limiting membrane. On the other hand noradrenaline granules were characterized by the presence of irregular shape with high electron density. This granules showed a wide lucent between the contents and limiting membrane. These two types of granules were co-existed in one cell in the late gestation and at birth, but after that two types of cells were clearly observed. The different sizes and numbers of granules were observed with ages.

• Korean Journal of Microbiology
• /
• v.23 no.4
• /
• pp.265-270
• /
• 1985

Overall procedure of cell fusion between Brevibacterium flavum and Corynebacterium glutamicum was morphologically observed by transmission electron microscopy. Protoplasts formed by treatment of cells with penicillin G and lysozyme in order were released through the pores generated on a certain region of cell walls to be spherical form. When two different protoplasts were met, cell wall and membrane in the contact zone was disappeared and followed by the mutual exchange of cytoplasmic and/or chromosomal materials. Cell xall regeneration speed of the protoplasts fused was slower than that of the non-fused, whereas the size of the former was confirmed as bigger than that of the latter.

• Journal of Convergence for Information Technology
• /
• v.9 no.6
• /
• pp.211-217
• /
• 2019

In this study, the change of skin condition was analyzed by skin analysis equipment using scrub cosmetics, and the morphological characteristics of microbead were observed by dermascope and scanning electron microscope. In the dermascope observation, during the process of cleansing, the microbeads attached to the skin existed in close contact with each other or dispersed. The skin after scrubbing was clean and smooth and the fine wrinkles between epidermal keratinocytes were reduced. In the scanning electron microscopic observation, the microbead surface did not have severe bending or rough surface. The skin moisture and oil content were higher than the scrub skin before the scrub, and there was no significant difference in the pH.

• The Journal of the Korean Society for Microbiology
• /
• v.5 no.1
• /
• pp.9-18
• /
• 1970

The present study is of ultra-fine structures of Trichophyton rubrum, Trichophyton mentagrophytes, Microsporum canis and Epidermophyton floccosum by means of electron microscopy and reveals the following. 1. In contrast to the bacteria, the normal fungus contains nuclear membrane, mitochondria, endoplasmic reticulun, distinct cell wall and cell membrane and secretory granules as observed in the higher plants and animals. 2. Thickening of the cell wall, inapparent cell wall, inapparent cell membrane with the appearance of electron thin area(ETA) and increase of inclusions were observed in the L-1 treated groups. 3. Thickening of cell wall and increase of ETA were more apparent in the Epidermophyton floccosum than the other groups. 4. Increase of electron thin area was thought to be associated with autolysis.

• Applied Microscopy
• /
• v.8 no.1
• /
• pp.9-15
• /
• 1978

Five strains of the Genus Rhizobium were isolated from the nodules of five leguminous plants respectively. They were identified according to Bergey's Manual together with the results of Vincent. The flagella of each strains were observed by electron microscope using negative staining with PTA and metal shadowing with chromium. Five host plants and identified Rhizobium strains were as. follows. Pisum sativum.....R. leguminosarum Phaseolus vulgaris.....R. phaseoli Trifolium repens.....R. trifolii Glycine max.....R. japonicum Lupinus grandiflorus.....R. lupini Electron micrographs showed that R. leguminosarum and R. phaseoli had 4 peritrichous flagella, where as R. trifolii had 5 peritrichous flagella. On the other hand, R. japonicum and R. lupini had 1 subpolar flagellum.

• Korean Journal of Veterinary Research
• /
• v.22 no.2
• /
• pp.187-195
• /
• 1982

This paper dealt with the light microscopical and electron microscopical findings on the morphological changes of the liver of Korean native goat injected with toxin (culture filtrate) of Clostridium perfringens which was isolated from Korean native cattle died of acute Clostridium perfringens enterotoxemia. The results observed are summarized as follows. In the microscopical findings, hyperemia and minute hemorrhage of the liver parenchyma, dilatation of hepatic central vein and centrilobular necrosis of liver, cloudy swelling and hydropic degeneration of hepatic cells, and appearance of light eosinophilic granular bodies in the vacuoles were recognized. In the electron microscopical findings, appearance of pinocytotic vesicle (coated vesicle), fusion of these vesicles, formation of vacuole and accumulation of minute granular proteinous materials in the vacuole were observed in the hepatic cells. Decreased number of glycogen granules, swelling and destruction of mitochondria, proliferation of smooth-surfaced endoplasmic reticulum, enlargement of rough-surfaced endoplasmic reticulum, dispersal of thready agranular membranous structure and appearance of secondary lysosome were recognized in the hepatic cell cytoplasm.

• The Korean Journal of Mycology
• /
• v.27 no.5 s.92
• /
• pp.341-344
• /
• 1999

The genus Hypocrea, a member of the Hypocreales, has yellow to pale yellow-ochre, perithecial stromata and angular to cuboid ascospores. The isolate of Hypocrea microrufa collected on decayed tropical wood at Malaysia is compared with H. rufa and H. microsplendens. This isolation of H. microrufa will be the first record from tropical region and the first description with scanning electron microscopy. The ornamentation of ascospores used one of main taxonomic keys for identification.

• Journal of Sericultural and Entomological Science
• /
• v.16 no.2
• /
• pp.111-117
• /
• 1974

The electron micrographs of the larval brain of the silkworm. Bembyx mori. show that median neurosecretory cell of diapause-egg producer may participate in the production of lipo-granules and that of non-diapause egg producer may do in the production of electron-translucent vesicles. It was found that ribosome-like particles of diapause-egg producer gradually developed into highly dense particles and came into line along the smooth-surfaced endoplasmic reticulum. They finally became lipo-granules.

• Korean Journal of Microbiology
• /
• v.22 no.1
• /
• pp.19-28
• /
• 1984

The nematode-trapping process by Arthrobotrys conoides was investigated with the aid of scanning and transmission electron microscopy. 1. A. conoides captures nematode by means of three-dimensional network. 2. The wall of trap cell was thicker than that of vegetative hypha and the trap cell was more rich in cell organelles such as endoplasmic reticulum, mitochondria and electrondense granule. 3. The electron-dense granule, which could be found only in trap organs, gradually disappeared during its penetration into nematode cuticle. 4. The osmiophilic area was found at adhering site between the trap organ and nematode cuticle. 5. In some cases, any appressorium was not found at the site of penetration. 6. When the fungal-nematode culture was conserved for 2~3 weeks, numerous young nematodes were found to be adhered to spores, resulting in death.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
• /
• 2004.10a
• /
• pp.380-383
• /
• 2004

본 연구는 원유에서 지방을 제거한 탈지유의 pH를 5.5, 7.0, 8.5로 조정한 다음 TGase를 첨가하여 0, 1, 2, 4, 8시간 반응시킨 다음 단백질 입자들을 동결건조하여 조직의 성상에 대해 주사 전자 현미경을 이용해 관찰, 비교하였다. pH와 TGase를 처리하지 않은 원유의 탈지유는 단백질 입자들이 규칙적으로 회합해 있었다. 그러나 pH 조정 후 TGase를 처리한 다음 반응시간을 달리한 시료에서는 pH를 5.5로 조정한 시료에서 현저한 변화가 있었는데 그 변화 양상은 단백질 입자들이 0시간에서 조각을 이루워 회합되어 있다가 1시간 반응시킨 경우 단백질 입자들이 서로 결합하여 넓게 회합을 하였다. 2시간 반응시킨 경우 단백질 입자들이 다시 뭉쳐서 회합하였으며 4시간 반응시킨 경우 뭉쳐져 있던 단백질 입자들이 조그만한 구형 성상으로 넓게 회합하였다. 8시간 반응시킨 시료는 구형 성상으로 회합되어 있던 단백질 입자들이 사라지면서 다시 넓게 회합하는 것을 관찰할 수 있었다. pH 7.0과 8.5 조건하에서는 단백질 입자들이 조각 형태를 이루고 있었으며 반응시간이 증가할수록 입자들이 넓게 확대되는 현상을 나타냈다. 이와 같은 단백질들의 변화 양상은 pH와 TGase처리 그리고 반응시간에 영향을 받고 있는 것으로 사료된다.