• Journal of Veterinary Clinics
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• v.32 no.2
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• pp.158-161
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• 2015

Bacterial dermatitis is common disease that is necessary to treat with antibiotics. In recent, antibiotic-resistant bacteria is being increased in worldwide. The purpose of the present study was to evaluate the prevalence of resistant genes in Staphylococcus (S.) pseudintermedius isolated from dogs, and to compare the resistant gene profile with the result of antibiotic disc diffusion test. A total of seven S. pseudintermedius was included in the study. Bacterial identification was performed by 16S ribosomal RNA gene sequence analysis. S. pseudintermedius isolates had more than one antibiotic resistant gene (mecA, blaZ and aac(6')/aph(2"). While all isolates were PCR positive to blaZ gene, only two isolates were resistant to amoxicillin/clavulanate. Among five isolates harboring gentamicin resistance, one isolate was negative to aac(6')/aph(2")-targeted PCR. Taken together, the results suggest that resistant gene-targeted PCR and disc diffusion test are complementary to detect antibiotic resistance.

• The Korean journal of helicobacter and upper gastrointestinal research
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• v.18 no.4
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• pp.277-279
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• 2018

전 세계적으로 Helicobacter pylori의 항생제 내성률은 지속적으로 증가하고 있으며, 기존의 제균 치료에 실패한 H. pylori 감염 환자들에 대한 효과적인 구제요법(rescue therapy)의 필요성 역시 증가하고 있다. 이 연구는 두 개 기관, 공개, 평행 그룹, 무작위 배정 연구로서 불응성 H. pylori 감염 환자들의 구제요법으로 유전자형 내성을 기반한 치료(genotype resistance-guided therapy)와 경험적 치료(empirical therapy) 중 어느 것이 보다 효과적인지를 비교하고자 하였다. 2012년 10월부터 2017년 9월까지 20세 이상의 불응성 H. pylori 감염 환자들을 대상으로 하였으며, 불응성 H. pylori 감염은 과거 두 종류 이상의 H. pylori 제균 치료를 받았음에도 불구하고 H. pylori 제균에 실패한 환자들로 정의하였다. 이들에게서 한 군은 14일간의 유전자형 내성을 기반한 순차 치료(n=21 in trial 1, n=205 in trial 2)를, 다른 한 군은 환자들의 과거 제균 치료 종류를 감안한 14일간의 경험적 순차 치료(n=20 in trial 1, n=205 in trial 2)를 시행하였다. 순차 치료법은 첫 7일은 esomeprazole 40 mg과 amoxicillin 1 g을 하루 두 번 복용한 다음, 나머지 7일은 esomeprazole 40 mg과 metronidazole 500 mg, 그리고 1) levofloxacin 250 mg 또는 2) clarithromycin 500 mg 또는 3) tetracycline 500 mg을 하루 두 번 복용하는 것으로 구성하였다. 23S ribosomal RNA (rRNA)나 gyrase A에 대한 내성 관련 돌연변이 여부는 direct sequencing을 통한 중합효소연쇄반응(polymerase chain reaction, PCR) 검사를 이용하였고, 제균 성공 여부는 요소호기검사를 통해 확인하였다. 일차 결과 지표는 치료 방법에 따른 제균율로 정하였다. Trial 1에서는 tetracycline 대신 doxycycline 100 mg을 사용하였는데, 제균 성공률이 유전자형 내성을 기반한 치료군에서는 17명(81%), 경험적 치료군에서는 12명(60%)으로 나타났다(P=0.181). 하지만, 다른 순차 치료군들과 비교하였을 때, doxycycline을 포함한 순차 치료군의 제균율이 현저히 낮은 것으로 나타나서(15/26, 57.7%) doxycycline을 포함한 순차 치료법은 종결하기로 하고, trial 2부터는 doxycycline 대신 tetracycline으로 교체하여 연구를 지속하였다. Trial 2의 intention-to-treat (ITT) 분석 결과, 유전자형 내성을 기반한 치료군에서는 160/205명(78%), 경험적 치료군에서는 148/205명(72.2%)으로 두 그룹 간의 통계적인 제균율의 차이는 보여주지 못하였다(P=0.170). 부작용 및 환자 순응도에서도 양 군 간의 의미 있는 차이는 없었다. 따라서, 두 종류 이상 H. pylori 제균 치료에 실패한 환자들이라고 할지라도 기존의 제균 치료력을 바탕으로 적절한 경험적 치료를 시행하는 것은 유전자형 내성을 기반한 치료 정도의 효과는 있으며 접근성, 비용, 환자들의 선호도 등의 여러 가지 부가적인 사항들을 고려할 때, 제균 치료력을 고려한 경험적 치료는 간단한 수준의 유전자형 내성을 기반한 치료의 대안으로 받아들여질 수 있을 것으로 제안하였다.

• Review of KIISC
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• v.31 no.1
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• pp.57-64
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• 2021

기존의 공개키 암호가 양자 알고리즘에 취약함이 밝혀지고 양자컴퓨터의 개발이 현실화됨에 따라 NIST는 미연방표준 양자 내성 암호 공모전을 실시하고 있다. FALCON은 공모전 Round 3까지 통과한 전자서명 알고리즘으로 서명 및 검증 속도가 빠르고 공개키 및 서명 길이가 짧다는 장점이 있다. 하지만 FALCON은 부동소수점 연산 등 특별한 구조로 설계되어 새로운 형태의 부채널 공격이 존재할 수 있다. 본 논문에서는 FALCON에 대한 세 가지 전력 분석 공격의 가능성을 제시한다. 또한 주어진 공격을 활용하여 개인키를 복원하는 방안에 대해서 제시한다.

• Journal of Yeungnam Medical Science
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• v.10 no.1
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• pp.28-36
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• 1993

Major pathogenic Gram-negative organisms such as P. aeruginosa, Serratia species, E. coli, Enterobacter species which are isolated from the specimens in large medical centers are greatly resistant to the commonly used antibiotics. Gram-negative bacilli, which had been isolated in Yeungnam University Hospital during the period from December 1992 to April 1993 and turned out to be resistant to the primary antibiotics susceptibility test for chloramphenicol, ampicillin, cephalothin, gentamicin, tetracyclin, amikin and tobramycin, were subjected to the secondary antibiotics susceptibility test for aztreonam, ceftazidime, ciprofioxacine, cefotaxime, cefamandole, piperacillin, ticarcillin and sulfamethoxazole trimethopime. Out of 315 tested organisms, 167 organisms(53%) were resistant to all secondary antibiotics in vitro. Antimicrobial activity of ceftazidime(37.1%), aztreonam(11.%), ciprofioxacine(7.9%) against Gram negative bacilli were slightly more active than other antibiotics tested, while cefamandole was not active to all the Gram-negative bacilli tested. According to the specimens, E. coli was the most frequently resistant organisms to the primary antibiotics from urine, A. baumanii, from respiratory system and wounds, and P. aeruginosa from various specimens. In summary, Gram negative bacilli resistant to the primarily applied antibiotics also were resistant to the secondary antibiotics. Rearrangement of the antibiotics disks for the antibiotic susceptibility test should be considered.

• Proceedings of the Korea Water Resources Association Conference
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• 2010.05a
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• pp.943-946
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• 2010

과거 시행된 수질오염물질의 농도규제 정책은 오염배출업소 증가로 인한 오염총량의 증가를 억제할 수 없어 수질개선에 한계가 나타났다. 이에 정부는 배출농도 규제방식의 수질관리로 4대강 상수원의 수질개선이 어려워 4대강 특별법 제정과 함께 목표수질기준 한도에서 유역의 오염물질 배출량을 총체적으로 관리하는 오염총량관리제도를 도입하였고, 현재 수질오염총량관리제도의 안정적 시행을 위해, 제 2단계 수질오염총량관리 대상물질 선정연구, 4대강 수계 수질오염총량관리 유황별 유달율 산정 방법 연구 등이 시행되고 있다. 이와 같은 오염총량관리를 위해서는 먼저 유역의 오염물질 발생현황과 배출 기작을 정량적으로 규명하고, 수질모델링을 실시하여 오염배출원별로 적정부하량을 할당하여야 한다. 이에 본 연구에서는 향후 활용도가 클 것으로 기대되는 QUALKO2 모형을 이용하여 TMDL시스템을 지원하고 낙동강의 수질을 예측 평가 하고자 한다. 대상유역으로는 영주 다목적댐이 위치하게 되는 내성천과 낙동강을 선정하였으며 모의 입력자료로는 최근 3년간의 평균수질을 비교대상인 현재 상태로 설정하고, 해당유역의 발생배출량에 따라 2014년, 2019년, 2024년의 저수지 모의를 통해 하천모의의 입력자료로 사용하였다. 수질모델 적용을 위해 내성천이 유입되는 지점에서 8km상류의 예천(환경부 측정망)지점에서부터 양산천 유입 후 3km 지점까지 범위를 설정하였으며 모델 구간은 "낙동강수계 오염총량관리 기본계획" 수립시 적용한 구간을 고려하여 구성하였다. 내성천 상류 영주댐 건설 지점에서부터 낙동강 본류로 합류되기 전의 구간과 낙동강 본류 구간을 구분하였으며, 수리학적 지형학적 특성을 고려하여 구간(reach)으로 구분하고 각 구간을 1km 간격의 요소(element)로 세분화하여 총 96여개의 구간과 482여개의 요소로 구성하였다. 영주댐이 건설되는 가정하에 낙동강 본류의 유량조건별, 영주댐의 방류량 조건에 따른 내성천과 낙동강 본류의 수질변화 양상 분석결과, 저수시 보다는 갈수시에 수질농도의 저감효과가 크게 나타났으며, 영주댐의 연평균방류보다는 최대방류시에 내성천과 낙동강 본류의 저감효과가 큰 것으로 분석되었다. 또한 영주댐 건설로 인한 flushing 효과와 낙동강 상류의 안동댐과의 연계시에 낙동강에서 저감효과가 가장 크게 나타났다.

• Tuberculosis and Respiratory Diseases
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• v.42 no.6
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• pp.831-837
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• 1995

Background: We conducted a study to determine the factors associated with, patterns of, and proportion of cases of pulmonary tuberculosis with multiple drug-resistance at Kosin medical center in Pusan. Methods: We abstracted data from 141 patients, who had active pulmonary tuberculosis and report forms of drug susceptibility between 1986 and 1994, and related the previous treatment history, the extent of lung involvement and the presence of cavities on chest X-ray films to the drug resistance. Results: Overall, 59(41.8%) of the 141 cases of tuberculosis were resistant to at least one drug and 29(20.9%) of the 139 cases were resistant to isoniazid(INH) and rifampin(RIF). Among the 63 patients with previous tuberculosis therapy, 40(63.5%) had isolates that were drug-resistant and 24(38.1%) were multi-drug resistant. Among the 78 without previous therapy, 19(24.4%) had isolates that were drug-resistant and 5(7.5%) were multi-drug resistant. For all 141, resistance to INH was most common(39.0%) followed by RIF(21.6%), ethambutol(EMB, 16.3%), $\rho$- aminosalicylic acid(10.8%), streptomycin(SM, 8.7%), and pyrazinamide(PZA, 8.0%). INH, RIF and PZA resistances were independently associated with a history of previous tuberculosis therapy (odds ratio; 3.3, 7.2 and 10.8 respectively), and RIF and SM resistance were significantly high according to the extent of lung involvement on the chest films(odds ratio; 2.9 and 2.8 respectively). Conclusions: We conclude, (1) that all persons in whom pulmonary tuberculosis is diagnosed should initially receive at least four-drug therapy(INH, RIF, PZA, and EMB or SM), (2) that susceptibility testing be done in all culture-positive patient, and (3) that those with a history of previous tuberculosis therapy or those who have advanced pulmonary tuberculosis need very careful clinical and microbiological follow-up.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.6
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• pp.413-419
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• 2021

The purpose of this study was to investigate the ability to treat and prevent infection by multiple Gram-negative bacterial pathogens as a last choice option in the treatment of serious infections in clinical settings. The global spread of extended-spectrum 𝛽-lactamases (ESBLs) and/or carbapenemases in microorganisms are of enormous concern to health services because they are often associated with multi-drug resistance which significantly restricts the antibiotic treatment options. In this study, the antimicrobial resistance profiles of bacteria isolated from South Korean market-derived meat samples were determined by the disc diffusion method. PCR was used to detect the presence of antibiotic resistance genes and ESBL producing genes. In total, we tested 181 isolated colonies from 36 market-derived meat samples. Single PCR and DNA sequencing results revealed that genes blaVIM, blaBIC, blaKPC, and blaSIM were present in the bacteria isolated from retail meat. The bacteria in the meat were separately sequenced and based on alignment, four different bacteria were identified. These findings suggest that bacteria found in retail meats are a reservoir for the spreading of ESBL blaVIM, blaBIC, blaKPC, and blaSIM resistance genes and bacteria strains.

• Korean Journal of Environmental Agriculture
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• v.16 no.4
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• pp.311-319
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• 1997

Heavy metal-tolerant microorganisms, such as Pseudomonas putida, P. aeruginosa, P. chlororaphis and P. stutzeri which possessed the ability to accumulate cadmium, lead, zinc and copper, respectively, were isolated from industrial wastewaters and mine wasewaters polluted with various heavy metals. Metal binding sites in the cells were investigated by extracting the components of the cells through pretreatments with hot water, acid, alkli, chloroform-methanol or chloroform-methanol/concentrated alkali. The heavy metal accumulation was drastically decreased by pretreatment with alkali or chloroform-methanol/concentrated alkali, but the heavy metal accumulation was not changed by pretreatment with chloroform-methanol. The amount of heavy metal accumulation was remarkably decreased by decreasing crude protein remaining in the cell. These results suggested that proteins of cell components played an important role on the heavy metal accumulation.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.33 no.5
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• pp.709-719
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• 2023

Recently, Post-Quantum Cryptography (PQC), which is secure against attacks using quantum computers, has been actively studied. In 2022, the KpqC competition, a competition for domestic PQC standardization, was launched, and a total of 16 candidate algorithms were received, and the first round is underway. In this paper, we apply Alexander May's Meet-LWE attack to TiGER, a lattice-based key encapsulation mechanism that is a candidate for the first round of the KpqC competition, and analyze its concrete attack complexity. The computational results of applying the Meet-LWE attack to each of the proposed parameters of TiGER show that the proposed TiGER192 parameter, which targets 192-bit quantum security, actually achieves 170-bit classical security. In addition, we propose a parameter setting to increase the attack complexity against the Meet-LWE attack.

• The Journal of the Korean Society for Microbiology
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• v.14 no.1
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• pp.27-37
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• 1979

Ninety-five strains of Shigella, 70 of Salmonella paratyphi A, and 230 of Salmonella typhi were tested for their resistance to drugs. Also studied was the inhibition and elimination of drug resistance. All except one strain of Shigella consisted of 79 Sh. flexneri and 16 Sh. sonnei were multiply resistant to chloramphenicol, tetracycline, streptomycin, and splfisomidine. Among them, 70 strains were resistant to ampicillin and carbenicillin, 80 to trimethoprim-sulfamethoxazole, 22 to nalidixic acid, and one to kanamycin, but strain resistant to gentamicin, cephaloridine, and rifampin was not encountered. All strains of S. paratyphi A and S. typhi were susceptible to drugs tested, except sulfisomidine and rifampin, for which all S. paratyphi A were slightly resistant to sulfisomidine and the majority of S. paratyphi A and S. typhi were slightly resistant to rifampin. Approximately 80% of multiply drug-resistant Shigella transferred their resistance to E. coli by conjugation, and the resistance was considered to be mediated by R plasmids. The frequency of transfer of drug resistance varied by donor strains and recipients, but not by selecting drugs. Resistance to nalidixic acid was not transferred by conjugation to the recipients. Drug-resistant Shigella strains successively subcultured in nutrient agar stabs contained clones resistant to drugs and those susceptible to drugs, but the ratio of resistant and susceptible clones varied by strains. The multiply drug-resistant S. typhi and Shigella strains were found to not lose completely their drug resistance by subculture in media. Acriflavine has some effect on the elimination of drug resistance mediated by R plasmids, but the effect varied markedly by strains. Atabrine has no effect among strains tested. The combination of drugs increased the drug actions in majority of cases with synergistic or additive effects.