• Proceedings of the KSAR Conference
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• 2003.06a
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• pp.70-70
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• 2003

조류의 배자는 포유류의 배자처럼 모체로부터 영양분을 공급받는 것이 아니라 알속에 저장되어 있는 영양물질로 발육한다. 배자의 발육은 대부분 체외에서 진행된다. 난자는 배란된 후 수정되어 난관팽대부에서 1세포기 수정란이 된다. 그 후 난관협부로 이동하여 최초로 분열이 일어나 배자의 발육이 진행되고, 산란시에는 세포수가 약6만여 개에 달한다. 따라서 수정란에 유전조작기법을 사용하기 위해서는 모체의 난관속에서 일어나는 배발생과 난각속에서 일어나는 개체발생을 위한 체외배양법과 대리난각 배양법이 확립되어 있어야 한다. 그와 같은 기술은 닭 수정란의 배 발생 관찰 및 분석과 유용한 물질을 생산하기 위한 형질전환 가금 연구에 중요한 기술로 사용되고 있다. 따라서 본 연구는 1세포기 닭 수정란의 체외배양과 대리난각 배양에 있어서 수정란의 조건과 대리난각의 조건이 배자의 생존율과 부화율에 미치는 영향을 조사하여 체외배양과 대리난각 배양에 의한 병아리 생산 효율을 제고하기 위한 기초자료를 얻고자 실시하였다. 주요 조사 항목은 수정란의 채란위치, 배자의 발생단계, 수정란의 무게, 대리난각용 계란의 보존 기간, 대리난각의 두께, 대리난각 두께의 감소율, 대리난각의 크기 등을 조사하여 배자의 생존율과 부화율에 미치는 영향에 대하여 조사하였다. 본 실험의 결과는 초기 발생이 빠른 배자가 생존율과 부화율이 높았으며, 본 실험에 사용한 대리난각용 계란의 보관기간이 짧을수록 배자의 생존율과 부화율이 높은 것으로 조사되었다(p<0.05). 그러나 대리난각용 계란의 크기와 대리난각의 두께 차이는 배자의 생존율과 부화율에 큰 영향이 없는 것으로 조사되었다.하였을 때 분할율은 68.0%였으며, 이중 12.0%가 상실배 또는 배반포로 발달하였다. 뿐만 아니라 10% FBS가 첨가된 TCM-199 배양액에 난관상피세포와 공배양을 실시하였을 경우는 72.0%가 분할하였으며, 이중 16.7%가 상실배 또는 배반포로 발달하였다. 이상의 결과로 볼 때 활성화 처리는 ionomycin과 6-DMAP 용액처리가 적합하며, 단위발생란의 체외배양은 보다 적합한 배양조건의 확립이 필요한 것으로 생각된다.icalcium lactate 공동배양은 체외배양에 별다른 영향을 미치지 못하는 것으로 나타났다.생산하는 것을 확인할 수 있었다.4시간에 등급별 회수율이 각각 GI(27.4%), GII(14.7%), GIII(43.2%), GIV(14.7%)로 나타났으며, 46~50시간에는 GI(12.0%), GII(12.0%), GIII(28.0%), GIV(48.0%)로 나타났다. 이상의 결과로 볼 때 미성숙 난자의 회수는 hCG 투여 후 29~34시간이 적합한 것으로 생각된다. 가금의 생산에 있어서 매우 효율적이고 주목할 만한 방법으로 사료된다. 것으로 나타났다.적외선.열풍 복합건조방법이 높게 나타나 이것은 곡물 표면에 원적외선 방사에의한 복사열이 전달되어 열장해를 받았기 때문으로 판단되며, 금후 더 연구하여 적정 열풍온도 및 방사체 크기를 구명해야 할 것이다.으로 보여진다 따라서 옻나무 유래 F는 포유동물의 생식기능에 중요하게 작용하는 것으로 사료된다.된다.정량 분석한 결과이다. 시편의 조성은 33.6 at% U, 66.4 at% O의 결과를 얻었다. 산화물 핵연료의 표면 관찰 및 정량 분석 시험시 시편 표면을 전도

• KSBB Journal
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• v.20 no.4
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• pp.323-327
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• 2005

RT-PCR is an useful method to investigate the expression of target gene as detection tools. Although RT-PCR is the powerful detection method for tissues, it was difficult to amplify the target gene product using the single cell. To clarify the expression level of the genes related to Parkinson's disease (PD), I performed the laser dissection of single cell from Substantia nigra. I examined the mRNA expression level in the dopaminergic neuron isolated from the PD patients by the single cell RT-PCR method. It is known that tyrosine hydroxylase (TH), DOPA decarboxylase (DDC) are involved in biosynthesis of the catecholamine such as dopamine. Little has been known about the gene expression features of these enzymes in single dopaminergic neuron. I could detect the specific gene products in single cell level. The different expression was observed in PD-related gene products from the single neuron of PD patients. Interestingly, TH gene expression was significantly decreased with comparing the ratio of decrease in other PD-related genes. Hence, I represented data that indicate the RT-PCR method described in this report is an effective method in detecting a specific single-cell mRNA level related with diseases.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.48 no.2
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• pp.127-134
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• 2020

High supersonic aircraft are exposed to high temperature environments by aerodynamic heating during supersonic flight. Thermal protection system structures such as double-panel structures are used on the skin of the fuselage and wings to prevent the transfer of high heat into the interior of an aircraft. The thin-walled double-panel skin can be exposed to acoustic loads by supersonic aircraft's high power engine noise and jet flow noise, which can cause sonic fatigue damage. Therefore, it is necessary to examine the behavior of supersonic aircraft skin structure under thermal-acoustic load and to predict fatigue life. In this paper, we designed and fabricated thermal-acoustic test equipment to simulate thermal-acoustic load. Thermal-acoustic testing of the titanium specimen under thermal-acoustic load was performed. The analytical model was verified by comparing the thermal-acoustic test results with the finite element analysis results.

• Korean Journal of Social Welfare
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• v.56 no.1
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• pp.23-43
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• 2004

The purpose of this paper is to evaluate the performance of New Deal under the New Labour government in Britain and examines the nature of New Deal with respect to workfare. The time difference of five years after the New Deal was put into effect shows that New Deal has contributed not only to include the socially excluded groups such as the young unemployed, the long-term unemployed, single parents, and the disabled into the labour market, but also to decrease the amount of income-based benefits providing for working generation. It can be said that the nature of New Deal under the New Labour is near to human capital development model rather than labour force attachment model. New Deal provides the opportunity of policy learning for the countries which pursue the reform of social security system to moving welfare beneficiaries being able to work into jobs. Policy learning can be summed up as follows. First, imposing mutual responsibility and obligations on unemployed person should be accompanied by implementing active labour market programmes of education and job training. Second, the delivery system which administrates workfare programmes should be decentralized in a local society. The cooperation between local government and enterprisers will be critical in implementing various employment programmes and moving unemployed person into jobs. Third, the case management for individual participating in workfare programme is necessary. The personal adviser should continue to provide employment services for the unemployed until he or she get a job and enter the state of self-reliance. Finally, the workfare programme should be firmly backed by the political leadership in order to overcome the oppositions of beneficiary groups under the existing social security system.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2011.10a
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• pp.824-827
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• 2011

Needs for Smart Grid development are increasing all over the world as a solution to its problem according to depletion of energy resources, climatic and environmental rapidly change and growing demand for electrical power. Especially decentralized power is attracting world's attention. In this mood a new era for a unit scale of decentralized power environment is on its way in building. However there is a problem to have to be solved in the uniformity of power quality because the amount of power generated from renewable energy resources such as wind power and solar light is very sensitive to climate fluctuation. And thus this paper tries to suggest an energy management algorithm on basis of real time monitoring for meteorological data. The proposed EMS model embodies the method for predicting the power generation by monitoring and analyzing the climatic data and controling the efficient power distribution between the renewable energy and the existing power. The ultimate goal of this paper is to provide the technological basis for achieving zero-energy house.

• The KIPS Transactions:PartC
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• v.15C no.1
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• pp.37-44
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• 2008

In order to minimize the signaling delay for a resource reservation on the new routing path after the handover of Mobile Node(MN) is completed, it is important to discover the crossover node where the old and new routing paths meet. With the 크로스오버 노드 being found, the signaling messages only need to be transferred on the changed part of the end-to-end path. The crossover node is generally discovered using the end-to-end Session ID(SID) of the established session between MN and Correspondent Node(CN). However, in the Hierarchical Mobile IPv6(HMIPv6) network, if the Mobile Anchor Point (MAP) reserves the resource by aggregate with the Home Agent(HA), the crossover node discovery cannot be performed in the general way since the aggregate SID that has established between the previous MAP and HA is different from the that of the current MAP and HA after MN's handover. In this paper, we propose a mechanism to discover the crossover node within the tunnel between the MAP and the HA in an HMIPv6 network, assuming that the Next Steps in Signaling(NSIS) is deployed for the resource reservation and the aggregate reservation is applied over the MAP and HA tunnel. The local repair required for the change of path is performed upon the crossover node discovery. The simulation results show that the proposed scheme reduces the signaling delay for the reservation and outperforms the existing scheme with respect to throughput during the handover.

• Tunnel and Underground Space
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• v.29 no.3
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• pp.184-196
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• 2019

This study aims to extract a 3D image of micro-cracks generated by hydraulic fracturing tests, using the deep learning method and X-ray computed tomography images. The pixel-level cracks are difficult to be detected via conventional image processing methods, such as global thresholding, canny edge detection, and the region growing method. Thus, the convolutional neural network-based encoder-decoder network is adapted to extract and analyze the micro-crack quantitatively. The number of training data can be acquired by dividing, rotating, and flipping images and the optimum combination for the image augmentation method is verified. Application of the optimal image augmentation method shows enhanced performance for not only the validation dataset but also the test dataset. In addition, the influence of the original number of training data to the performance of the deep learning-based neural network is confirmed, and it leads to succeed the pixel-level crack detection.

• The Journal of the Convergence on Culture Technology
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• v.9 no.1
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• pp.249-255
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• 2023

World Heritage is a very important heritage that is worth protecting and handing down to future generations. In Korea, which has a long history and culture of 5,000 years, Seowon is a great advantage in that it can develop tangible and intangible Neo-Confucian ideas shared in China and East Asia, and preserve and experience them as a unique cultural resource of Korea until the present day. In order to encourage the direct experience of this long-standing cultural heritage, the government supports the national treasury and seeks to expand the demand for tourism. Accordingly, in this study, an analysis was conducted on program satisfaction, participation, and activation plans. As a result, it was found that value to see and value to experience must coexist in order for our world heritage, which requires comprehensive and continuous management and operation, and lacks accessibility, to become a tourist attraction. Also, for this purpose, promotion and development of differentiated tourism experience contents should be carried out first.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2023.05a
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• pp.250-251
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• 2023

The pallet, typically a form of tertiary packaging, is a flat structure used as a base for the unitization of goods in the supply chain. In addition, standard pallets such as T-11 and T-12 are used throughout the logistics industry to reduce the cost and enhance the efficiency of transportation. However, in the case of special cargo, it is impossible to handle such cargo using a standard pallet due to its size and weight, so many have developed and are now using their customized pallet. Therefore, this study suggests a pallet size optimization method to calculate the optimal pallet size, which minimizes the loss of space on a pallet. The main input features are the specifications and the storage quantity of each cargo, and the optimization method that has modified the Neighborhood Search Algorithm calculates the optimal pallet size. In order to verify the optimality of the developed algorithm, a comparative analysis has been conducted through simulation.

• Journal of the Korean Electrochemical Society
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• v.27 no.1
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• pp.32-39
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• 2024

The key components of a Phosphoric acid fuel cell (PAFC) are an electrode catalyst, an electrolyte matrix and a gas diffusion layer (GDL). In this study, we introduced a microporous layer on the GDL of PAFC to enhance liquid electrolyte management and overall electrochemical performance of PAFC. MPL is primarily used in polymer electrolyte membrane fuel cells to serve as an intermediate buffer layer, effectively managing water within the electrode and reducing contact resistance. In this study, electrodes were fabricated using GDLs with and without MPL to examine the influence of MPL on the performance of PAFC. Internal resistance and polarization curves of the unit cell were measured and compared to each other to assess the impact of MPL on PAFC electrode performance. As the results, the application of MPL improved power density from 170.2 to 192.1 mW/cm². MPL effectively managed electrolyte and water within the matrix and electrode, enhancing stability. Furthermore, the application of MPL reduced internal resistance in the electrode, resulting in sustained and stable performance even during long-term operation.