• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2004.05b
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• pp.307-310
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• 2004

전기영동전착법을 이용한 초전도 후막선재의 제작은 제작 장치와 공정이 간단하고 두께제어 및 다양한 형태의 초전도 막과 선재 제작이 가능한 경제적 효율성과 기술적 장점을 가지고 있다. 따라서 초전도 후막의 특성향상을 위하여 전착공정 과정에서 입자의 치밀성 및 배향성 향상을 위한 칙적화 방안과, 건조 및 열처리 과정에서 발생되는 크랙 및 기공현상과 같은 문제점을 극복 할 수 있는 균일한 후막 표면 화보에 관한 비교 분석 연구를 수행하였다. 전기영동전착법에 의한 초전도후막 제작을 위한 조건 중 현탁용매 조건에 대한 최적화를 구현함으로써 전착 불균일성에 따른 초전도 특성 저하요인인 후막표면의 기공과 크랙현상을 최소화하였다. 전기영동 전착법에 의한 초전도 후막의 특성을 향상시키기 위한 제작 방법으로 각 용매별에 따른 안정화된 제작 조건을 구현하였다.

• The Korean Journal of Mycology
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• v.16 no.2
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• pp.87-94
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• 1988

Electrophoretic isozyme patterns from mycelia, primordia, cap and stem of Pleurotus spp. collected in Korea were compared. Primordia, cap and stem of fruitbody showed very similar isozyme patterns but mycelial isozyme patterns were different from those of fruitbody. Isozyme patterns of malate dehydrogenase, acid phosphatase in Pleurotus ostreatus collected from different regions in Korea were similar but those of esterase, peroxidase, leucine amino peptidase and superoxide dismutase were different. Interspecific comparison of esterase isozyme patterns among Pleurotus ostreatus P. cornucopiae and, P. florida was very different and may be valuable subsidiary tool to conventional taxonomic techniques for identifying species of Pleurotus. A dendrogram by similarities of isozyme band pattern was presented.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.39 no.5
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• pp.425-433
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• 2015

In this paper, we have numerically investigated two-dimensional dielectrophoretic (DEP) motions of a single particle suspended freely in a viscous fluid, interacting with a nearby nonconducting planar wall, under an externally applied uniform direct-current electric field. Particularly, we solve the Maxwell equation with a large sharp jump in the electric conductivity at the particle-fluid interface and then integrate the Maxwell stress tensor to compute the DEP force on the particle. Results show that, under an electric field parallel to the wall, one particle is always repelled to move far away from the wall and the motion depends strongly on the particle-wall spacing and the particle conductivity. The motion strength vanishes when the particle is as conductive as the fluid and increases as the conductivity deviates further from that of the fluid.

• Electric Engineers Magazine
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• s.347
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• pp.14-15
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• 2011

• 한국전기화학회:학술대회논문집
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• 2005.04a
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• pp.41-41
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• 2005

• Electrical & Electronic Materials
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• v.18 no.5
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• pp.7-17
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• 2005

• Parasites, Hosts and Diseases
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• v.30 no.3
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• pp.209-218
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• 1992

When cystic fluid of Taenia solium metacestodes (CF) was filtrated through Sephacryl S-300 Superfine, major proteins were in fractions III add IV Major protein in fraction III was Band C protein of 150 kDa and that in fraction IV was Band N protein (Choi et of., 1990). When CF was electrophoresed in 0.9% agarose gel and reacted with anti-CF rabbit serum (RACF), two main bands, a long outer and a short inner band, were precipitated, together with 8 minor bands. RACF reacted with fraction III forming the long outer band whereas RACF formed the short infer band with fraction IV in immunoelectrophoresis (IEP) The long outer precipitin band of CF fraction III was similar to antigen B in hydatid fluid (HF) of Oriol et at. (1971), while the short inner band of CF fraction IV was similar to HF antigen 5 of Caption et at. (1967) . When HF was reacted with RACF, the short inner band was immunoprecipitated without forming the long outer band. Common antigenicity between CF and HF seemed to exist in fraction IV rather than in fraction III of CF. Patient sera of neurocysticercosis reacted more frequently with fraction III than with fraction IV.

• Journal of Life Science
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• v.29 no.12
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• pp.1408-1414
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• 2019

Enzymes are widely used in industrial applications such as detergents, food, feed production, pharmaceuticals and medical applications and major contributors to clean industrial products and processes. To screen, identify, and characterize the enzymes the zymography techniques are routinely used. The zymography technique is a simple, sensitive, and quantifiable technique that is widely used to detect functional enzymes following electrophoretic separation in sodium dodecyl sulfate (SDS)-polyacrylamide gels. The method is a versatile two-stage technique involving protein separation by electrophoresis followed by the detection of enzyme activity in polyacrylamide gels under non-reducing conditions. It is based on SDS-polyacrylamide gel (PAG) copolymerization with substrates, which are degraded by the hydrolytic enzymes restored in enzyme reaction buffer after the electrophoretic separation. Any kind of biological sample can be applied and analyzed on zymography, including culture supernatants of microbes, plants extracts, blood, tissue culture fluids, enzymes in foods extracts and metaproteome. The advantage of zymography is that it is possible to directly detect the protein with activity on the electrophoretic gel as well as confirm the activity at the nanogram level. Thus, this zymography technology can be applied in various fields. However, these advantages are rather disadvantageous and can often lead to experimental errors. In this review, the advantages, disadvantages, and problem solving of zymography technique are described.

• Journal of Oral Medicine and Pain
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• v.16 no.1
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• pp.9-23
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• 1991

이온 영동법은 전기력의 도움으로 이온화된 물질의 신체조직내 침투를 증가시키는 술식으로서 전신적 부작용은 줄어드는 반면, 국소부위의 약물농도를 증가시킬 수 있다는 장점 때문에 효과적인 국소요법으로 인정받고 있다. 치의학 분야에서는 과민상아질의 치료를 위해 불소 이온영동법이 빈번히 이용되어져 왔으며, 국소마취제나 항바이러스 제재의 도포시에도이용되었다.또,이온 영동법에 의한 스테로이드 투여로 피부나 구강점막의 염증성 질환의 효과적 치료를 보고한 많은 문헌이 있으나, 이온영동법에 의한 스테로이드의 구강점막에의 침투량이나 분포에 관해서는 거의 소개된 바가 없는 실정이다. 본 연구는 방사선 동위원소가 부착된 dexamethasone을 이온영동법을 이용하여 가토의 협점막에 침투시킨후 자기방사선 술식에 의해 그 침투량과 분포를 대조군과 비교 평가하였으며 다음과 같은 결과를 얻었다. 1. 이온영동법은 단순 국소도포에 비해 dexamethasone과 0.1M 인산소다 완충용액의 혼합액(dexamethasone in 0.1M sodium phosphate buffer solution)의 가토 협점막 침투량을 증가시켰으며, 양극을 사용하였을 때 더 효과적이었다. 2. Dexamethasone과 0.1M 인산소다 완충용액의 혼합액 투여 4시가, 24시간후 까지도 양극 잉온영동법이 효과적이었으며 은입자의 감소는 투여 4시간부터 24시간 후 사이에 주로 일어났다. 3. 인산소다 완충용액의 첨가는 양극 및 음극에 의한 이온영동법 모두에 효과적이었으며, 양극에 가장 효과적이었고 단순도포군에는 영향을 미치지 않았다. 4. 이온영동법에 의한 스테로이드 투여는 피부뿐만 아니라구강점막 염증성 병소의 효과적 치료술식으로 여거질 수 있다. 시와 maximal clenching시 사이의 치아 접촉시간에서도 유의한 상관관계를 보였다.

• Journal of the Korean Chemical Society
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• v.49 no.3
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• pp.255-260
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• 2005