• The Korean Journal of Zoology
• /
• v.38 no.1
• /
• pp.106-114
• /
• 1995

배추흰나비의 조직과 발생시기에 따른 발생 특이 큐티클단백질의 질적 분포를 조사하기 위해 전기영동법, western blotting 및 autoradiography 법을 사용하였다. SDS-PAGE에서 유충기에는 4개, 용기에는 3개의 단백질이 발생 특이 큐티클단백질로 확인되었다. 전기영동적 이동도로 보아 유충기의 62 Kd 큐티클단백질은 지방체 표피, 혈림프에 모두 분포하며. 22 Kd, 16 Kd 단백질은 지방체와 표피, 그리고 19 Kd 단백질은 지방체에서 확인되고 있으며, 용기의 37 Kd 단백질은 표피에서. 28 Kd, 27 Kd 단백질은 표피와 지방체에 분포하는 양상을 보이고 있다 면역학적 방법으로 발생 특이 큐티클단백질의 동질성을 조사한 결과 용기의 27 Kd 단백질은 표피와 지방체에서 반응을 나타내며, 유충기의 22 Kd 단백질은 단지 표피에서만 미약하게 이의 동질성을 보여주고 있다 즉, 27 Kd 단백질은 지방체 22 Kd 단백질은 표피에서 기원하고 있어 발생 특이 큐티클단백질의 합성부위는 한 조직에서 기원하는 것이 아니라 각기 상이한 조직으로 부터 생성되는 것으로 사료된다.

• Journal of Sericultural and Entomological Science
• /
• v.30 no.1
• /
• pp.20-24
• /
• 1988

Adult specific proteins, referred to as ASP-I(adult specific protein of slow mobility) and ASP-II(adult specific protein of slow mobility) at the pharate adult stage of the silkworm, Bombyx mori, were detected by polyacrylamide gel electrophoresis. The adult specific proteins (ASP-I and ASP-II) were defined as a kind of adult hemolymph proteins without sex specificity, and the was no variation in the respective electrophoretic mobility of ASP-I and of ASP-II among the forty-one silkworm varieties. tested.

• Korean journal of applied entomology
• /
• v.29 no.3
• /
• pp.170-175
• /
• 1990

These studies were conducted to examine the mechanism of acaricidal resistance in the twospotted spider mite (Tetranychus urticae Koch). The resistant strains were obtained by succssive selection of five acaricides including carbonphenothion and ethion of organophosphorus compound, dicofol of organochlorine compound, cyhexatin of compound and biphenthrin of synthetic pyrethroid. Esterase isozymes were separated by polyacrlyamide gel susceptible strains. The differences of the esterase isozymes of the resistant strains were Est. 1, Est. 3 in the carbonphenothion-selected strain, Est. 3 in the ethion- and the cyhexatin-selected strains, Est. 1, Est. 3, Est. 7 in the dicofol-selected strain, Est. 7 in the biphenthrin-selected strain as compared to the susceptible strain. With the difference of electrophoretic bands and their activities, esterases were related to the resistant mechanism of tested acaricides.

• Journal of Sericultural and Entomological Science
• /
• v.26 no.1
• /
• pp.35-51
• /
• 1984

In order to know the similarity of the electrophoretic protein patterns, distributional aspects of the frequency in each protein band, differences of protein patterns between female and male, and the number of the total protein bands of each variety in the haemolymph of the silkworm pupae according to their geographical origin, the silkworm varieties, and both the sexes, variable electrophoretic protein patterns were observed by polyacrylamide gel electrophoresis, using the silkworm strains which have been reared in the sericultural experiment station. 1. The total number of electrophoretic protein bands classified were twenty-eight, and nearly fourteen bands were shown by each variety on an average. 2. According to their geographical origin, the mean values of the similarity were 20.0%, 28.3% and 14.3% in Japanese, Chinese and European respectively, and the highest similarity was observed between Chinese varieties. 3. The similarity of 129 varieties were tested and it was 22.5%, 23.5% in female and male respectively. 4. The electrophoretic protein patterns in 129 varieties were tested and variation was found in 109 patterns in female and 112 in male. 5. The frequency of the each specified protein band in female and male having variation in protein bands in each variety was found averagely 6.6%. 6. The distribution frequency of the classified 28 protein bands in both the sexes within each geographical origin was variable from 0% to 100%. 7. Among the classified 28 protein bands, HP 11 protein band was found common in all the varieties tested. 8. HP 3 and HP 4 protein bands in female showed high density in electropherograms than in male.

• Journal of the Korean Geophysical Society
• /
• v.5 no.4
• /
• pp.329-336
• /
• 2002

Electrical resistivity dipole-dipole, seismic refraction, and seismic reflection methods were performed to delineate the boundaries the Yongdong basin(Cretaceous) in terms of physical properties and to ultimately identify the margin architectures of the faults or unconformities. Higer resistivities (approximately >2000 ohm-m) most likely originate from the basement of the basin, contrasting with the lower resistivities from infilled sedimentary rocks. Faults at the eastern margin and unconformities at the western boundary are characterized as high-slope($70^{\circ}$) and gentle-slope($30^{\circ}$) gradients in the resistivity sections, respectively Such features for the boundaries are also suggested by the lower values of seismic velocity and resistivity for the western margin.

• Korean journal of food and cookery science
• /
• v.7 no.3
• /
• pp.13-20
• /
• 1991

This study was performed to examine the electrophoretic properties of Job's tears (Yulmoo: Coix lachryma-jobi L. var. Ma-yuen Stapf. & Yeomjoo: Coix lachryma-jobi L.) proteins. Albumins, globulins, gliadins and glutelins were extracted from the polished Yulmoo and brown Yeomjoo by the modified Osborne method. For a comparison, rice proteins were extracted and fractionated by the same method. The relative proportions of protein fractions were 17.4 : 19.6 : 55.2 : 7.7% in polished Yulmoo, 12.6 : 62.2 : 4.2 :21.0% in brown Yeomjoo and 14.2 : 57 4 : 0.77 : 27.8% in rice, in the order of albumis, globulins, gliadins and glutelins. Polyacrylamide gel electrophoresis (PAGE) and SDS-polyacrylamide gel electrophoresis (SDS-PAGE) were peformed to identify the subfractions of each protein fraction extracted from polished Yulmoo, brown Yeomjoo and rice. The electro-phoregrams of polyacrylamide gel electrophoresis showed that the same fractions of both polished Yulmoo protein and brown Yeomjoo protein had very similar electrophoretic patterns to each other respectively, but there were significant differences in the patterns between Job's tears proteins and rice proteins.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
• /
• 2004.11a
• /
• pp.97-102
• /
• 2004

용액 중에서 나노입자의 전기영동 특성을 이용한 전자종이용 잉크 제조를 위해 $TiO_2$ 나노입자를 저유전율 용매인 cyclohexane에 혼합한 후 용매와 용질의 비중차를 줄이기 위해 분말 상 polyethylen을 첨가하여 high energy milling의 방법으로 입자분쇄와 동시에 입자 표면에 고분자 풍을 코팅하였다. 용액내의 입자 분산성 향상과 용매 착색을 위하여 계면활성제와 oil-blue N을 첨가한 후 전자종이용 잉크를 제조하여 측정한 제타 전위 결과 cyclohexane 내에서 $TiO_2$의 제타전위는 -40mV 정도였으나 polyethylene으로 코팅한 후 계면활성제를 첨가하였을 경우 최대 -110mV 이상의 높은 값을 나타내었다. 실제 디스플레이 특성을 평가하기 위해 포토리소그래피를 이용하여 3인치 크기의 ITO glass 위에 $10{\mu}m$의 크기를 갖는 십자형의 격벽을 $40{\mu}m$의 높이로 균일하게 형성한 후 합성된 전자잉크로 주입하여 상부전극과 하부전극사이에 UV 경화제를 도포하여 UV 접합을 실시하였다. 격벽 내에서 입자의 mobility를 측정하여 환산된 전자잉크의 응답속도는 0.1cm/sec로 측정되었으나, 전기영동시 입자들의 움직임에 따른 반사광의 파형을 측정한 경우 0.07cm/sec의 응답속도를 나타내었다.

• Journal of the Korean Institute of Gas
• /
• v.16 no.6
• /
• pp.7-16
• /
• 2012

Combustion Toxic Effects among several factors of risk encountered during fire are important in the evacuation and final survival, and they are broader and fatal than the direct damages caused by flame. Most studies on fire toxicity until the present are limited to fatality, mainly deaths by fire through pathological research. In this study, it is conducted as a fundamental experiment to address 3 principles of animal experiment and to provide an alternative test to animal testing that is regulated by national building codes and it was conducted through approval by the animal testing ethics committee. Hence, in this study average time of activity stop was measured after directly inhaling toxic gases (HCN) to laboratory animals (mice) through gas toxicity test (KS F 2271) for major asphyxiating gases(HCN) which are produced during fire combustion. effects of Combustion toxic gases on body were quantitatively analyzed through changes in internal organs and hematological analysis, and electrophoresis of a single cell of these laboratory animals. Biological conclusion of combustion toxicology is drawn through approaches (pathological examination, blood test, blood biochemical test, electrophoresis analysis of single cell) which could not confirmed in existing gas toxicity test.

• Korean Journal of Food Science and Technology
• /
• v.17 no.1
• /
• pp.1-4
• /
• 1985

For the systematic investigation of biochemical characteristics of Korean ginseng protein by years, protein fractions were analyzed by the techniques of polyacrylamide gel electrophoresis, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and gel filtration, while the amino acid composition was studied by amino acid autoanalyzer. Results of polyacrylamide gel electrophoresis and SDS-PAGE showed a few difference in pattern and number of bands depending on the age of the root. However, the number of bands obtained from polyacrylamide gel electrophoresis and SDS-PAGE was 8 and 7 to 11, respectively. When water extracted proteins were fractionated by Sephadex G-200, the main peak among 2 peaks was collected and lyophillized. Its mol. wt. was extimated to be 43,000 by the SDS-PAGE method. In amino acid composition of water extracted protein and main fraction of gel filtration, arginine content was the highest, 47.17% in water extracted protein and 57.36% in main fraction followed by glutamic acid and asparatic acid. On the contrary, cystine and methionine contents were very low in both cases.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.8 no.3
• /
• pp.679-685
• /
• 2004

Although the electrophoretic deposition method has the advantage of simple processing procedure, less fabrication facilities, and easier control for deposition thickness and wire length, providing economical and technical merits, it also has the disadvantages of cracking and porosity phenomena, requiring an improved processing method for higher particle density and constant particle orientation. we have developed an optimization method to increase the particle density and to unify its orientation, and have performed a study to overcome the cracking and porosity problems in the fabricated superconductor. In order to improve the surface uniformity and the conduction properties of the fabricated YBCO thick films, a system that applies alternate voltage vertically has been developed for the first time and applied to the electrophoretic deposition process. The applied alternate electric field caused a force to be exerted on each YBCO particle and resulted in a rotation of the particle in the direction of applied electric field, accomplishing a uniform particle orientation. We name this process as the shaky-aligned electrophoretic deposition method. For commercial utilization and efficiency, in this dissertation, alternating voltage of 60 Hz and 25 ∼ 120 V/cm was proposed to apply it as a subsidiary source for shaky-flow deposition so that the fabricated thin film showed uniform surface morphology with less voids and cracks and Tc,zero of 90 K and the critical current density of 3419 A/$cm^2$.