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http://dx.doi.org/10.7842/kigas.2012.16.6.7

A Study on DNA Degeneration by Comet Assay & Pathological Observation for Mouse Which were Exposed HCN Gases from Fire  

Cho, Nam-Wook (Fire Disaster Preventionresearch Center, Korea Institute of Construction Technology (KICT))
Oh, Eun-Ha (NEODIN Medical Institute)
Hwang, Sung-Kwy (Dept. of Fine Chemistry, Seoul National University of Science and Technology)
Publication Information
Journal of the Korean Institute of Gas / v.16, no.6, 2012 , pp. 7-16 More about this Journal
Abstract
Combustion Toxic Effects among several factors of risk encountered during fire are important in the evacuation and final survival, and they are broader and fatal than the direct damages caused by flame. Most studies on fire toxicity until the present are limited to fatality, mainly deaths by fire through pathological research. In this study, it is conducted as a fundamental experiment to address 3 principles of animal experiment and to provide an alternative test to animal testing that is regulated by national building codes and it was conducted through approval by the animal testing ethics committee. Hence, in this study average time of activity stop was measured after directly inhaling toxic gases (HCN) to laboratory animals (mice) through gas toxicity test (KS F 2271) for major asphyxiating gases(HCN) which are produced during fire combustion. effects of Combustion toxic gases on body were quantitatively analyzed through changes in internal organs and hematological analysis, and electrophoresis of a single cell of these laboratory animals. Biological conclusion of combustion toxicology is drawn through approaches (pathological examination, blood test, blood biochemical test, electrophoresis analysis of single cell) which could not confirmed in existing gas toxicity test.
Keywords
comet assay; animal test; pathological observation; combustion toxicology;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
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