• Horticultural Science & Technology
• /
• v.28 no.5
• /
• pp.719-727
• /
• 2010

Reduced irradiance promotes shoot elongation depending on developmental stage and environmental factors and decreases plant quality in $Cyclamen$ $persicum$ Mill. To determine the petiole elongation responses to low irradiance, 'Metis Scarlet Red' cyclamen at different developmental stages [juvenile (5-6 unfolded leaves), transitional (1-3 visible flower buds), or mature (1-3 elongating peduncles)] was grown in growth modules at 60 (low light, LL) or 240 (high light, HL) ${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD within the growth chambers at different temperatures [16/12 (low temperature, LT), 22/18 (medium temperature, MT), or 28/$24^{\circ}C$ (high temperature, HT) (day/night)]. In Experiment I, juvenile plants were either kept in an LL or HL module during the entire treatment of 4 weeks or were transferred to the other module at 1, 2, or 3 weeks after treatment in an MT chamber. In Experiment II, juvenile, transitional, or mature plants were moved to the HL module at 0, 3, 6, 9, or 12 days after being placed in the LL module at the MT chamber and grown for 21 days. In Experiment III, transitional plants were moved to the HL module at 0, 3, 6, 9, or 12 days after being placed in the LL module at the LT, MT, or HT chambers. As the exposure duration to LL increased from 0 to 4 weeks or from 0 to 12 days, petiole length and plant height increased at all temperatures and developmental stages. In Experiment I, the exposure to LL during the latter period, rather than the early period, increased elongation rate. In Experiment II, petiole elongation in transitional plants was more sensitive to LL than juvenile or mature plants during the early period of the treatment for 12 days. In Experiment III, petiole length increased with increasing temperature and exposure duration to LL. Petiole elongation rate at HT increased rapidly from the beginning of LL exposure as compared to LT. Increase of $6^{\circ}C$ in temperature had the similar effect to LL exposure for 3 days in petiole elongation. To conclude, transitional cyclamen under higher temperatures responds more immediately to low irradiance and elongates its petioles.

• Journal of Food Hygiene and Safety
• /
• v.27 no.3
• /
• pp.317-324
• /
• 2012

In order to determine an authenticity of food ingredient, we used DNA barcode method by universal primers. For identification of animal food ingredients, LCO1490/HCO2198 and VF2/FISH R2 designed for amplifying cytochrome c oxidase subunit1 (CO1) region and L14724/H15915 for cytochrome b (cyt b) region on mitochondrial DNA were used. Livestock (cow, pig, goat, sheep, a horse and deer) was amplified by LCO1490/HCO 2198, VF2/FISH R2 and L14724/H15915 primers. Poultry (chicken, duck, turkey and ostrich) was amplified by LCO1490/HCO 2198 and VF2/FISH R2 primers. But, Fishes (walleye pollack, herring, codfish, blue codfish, trout, tuna and rockfish) were only amplified by VF2/FISH R2 primers. For plant food ingredients, 3 types of primers (trnH/psbA, rpoB 1F/4R and rbcL 1F/724R) have been used an intergenic spacer, a RNA polymerase beta subunit and a ribulose bisphosphate carboxylase region on plastid, respectively. Garlic, onion, radish, green tea and spinach were amplified by trnH/psbA, rpoB 1F/4R and rbcL 1F/724R. The PCR product sizes were same by rpoB 1F/4R and rbcL 1F/724R but, the PCR product size using trnH/psbA primer was different with others for plants each. We established PCR condition and universal primer selection for 17 item's raw materials for foods and determine base sequences aim to PCR products in this study. This study can apply to determine an authenticity of foods through making an comparison between databases and base sequences in gene bank. Therefore, DNA barcode method using universal primers can be a useful for species identification techniques not only raw materials but also processed foods that are difficult to analyze by chemical analysis.

• Korean Journal of Food Science and Technology
• /
• v.12 no.4
• /
• pp.254-262
• /
• 1980

A series of experiment were carried out to separate the factor accelerating the lysis of cell wall of $Saccharomyces\;sak{\acute{e}}$ from the preparation of crude zymolyase obtained from Arthrobacter luteus. An attempt was also made to purify the enzyme which is essential for the study on the separation of the factor. The results are summarized as follows: 1. Crude zymolyase was fractionated 5 peaks $(A{\sim}E)$ containing three peaks $(A{\sim}C)$ passed through the column by the chromatography on Biogel CM-30. 2. Among the five peaks, peak E (protease fraction) was found to contain the factor accelerating the lytic activity of the zymolyase. 3. L-c fraction purified in almost free form from the nonlytic ${\beta}-1$, 3-glucanase, protease and inert protein by the affinity adsorption chromatography with Sephadex G-75 gel was obtained from zymolyase fraction (peak D). When it was subjected to polyacrylamide gel disc electrophoresis, only one clear protein band was observed at pH 4. 5, but still detected two or more band at pH 8. 3.

• Korean Journal of Agricultural Science
• /
• v.12 no.1
• /
• pp.75-85
• /
• 1985

This study is intended to find out the influence of Lignosulfonic Acid Type Admixture on compressive, tensile, flexural strength and dispersing action of mortar, and fixation of by-product of pulp industry. 1. The more Pozzolith-84 is added, the larger flow value is. The admixture of lignosulfonic acid type adhere to cement particles and the surface potential of particles is generated. On account of the repulsion among the cement particles, they are dispersed and the mortar get workable, so the production cost of precast product is curtailed and the amount of cement is reduced in a certain workability of mortar. 2. The strength of mortar is greater than plain mortar when P/C added is 0.2 and 0.4%. As time passed the potential energy is reduced and the distance of particles which lignosulfonic acid adhered to get near according as the amount of adhesion is increased. The setting and hardening reaction of morter is occurred in close state, so the strength of mortar is increased a little. The strength of mortar is less than plain mortar when amount P/C added is 0.8%. Pozzolith-84 is mainly composed of lignosulfonic acid and lignin does not influence the hardening of mortar, therefore the remained $SO_3$, $SO_3H$ are the reason of decrease of strength. 3. There is high significance between specific gravity and compressive strength. The larger specific gravity is, the more compressive strength is increased. There is high significance between 7 day's strength and 28 day's strength. The larger compressive strength is, the more tensile and flexural strength are increased. 4. Since Pozzolith-84 is a by-product of pulp industry, by using the Pozzolith-84 admixture the concreate quality is improved. The water pollusion is reduced according to fix by-products in concrete structure.

• Journal of Life Science
• /
• v.17 no.1 s.81
• /
• pp.82-90
• /
• 2007

In an attempt to establish the appropriate ventilation device for the bottle culture of king oyster mushroom (Pleurotus eryngii), we investigated carbon dioxide concentration and fruiting body formation according to the various ventilation systems within the mushroom house. In addition to, the efficiency of air circulation and growth rate as well as the appearance of physiologically abnormal phenotypes during their growth stage were also evaluated. four different ventilation devices, parallel-pressure type, positive-pressure type, negative-pressure type, and positive- and negative-pressure type were applied in this study. The positive-and negative-pressure type showed the highest efficiency of air circulation as $CO_2$ concentration was 800 ppm and the level of air current was relatively low compared to the other types (the $CO_2$ concentration of parallel-pressure type was 1,400 ppm). Moreover, the stipe length, the cap diameter, yield, and general quality grown in positive- and negative type ventilation device were also better than in the other three devices though it took slightly longer period for harvesting (18.4 days) than the others (17.6, 17.9 and 18.3 days). The appearance of physiologically abnormal phenotypes such as fruiting body lump, soft rot, and brown rot were significantly decreased in positive-and negative type compared to other types, while the appearance rates were not much different for other symptoms of bacterial ooze, stipe limb and stipe bumpy. In summary, we propose that the optimal ventilation system for the bottle culture of king oyster mushroom is positive- and negative type, and this device is expected to increase the total quality as well as yield all year around.

• Korean Journal of Medicinal Crop Science
• /
• v.4 no.4
• /
• pp.294-300
• /
• 1996

We performed an experiment to confirm the possibility of wasabi culture using the wat­er of the Soyang River Dam in Chunchon and the ground water in Suwon. Water mineral content of Soyang River except for P was less than that of ground water of Suwon. Dis­solved oxgen and E C of Chunchon was proper to culture wasabi but E C and dissolved oxgen of Suwon was not suitable for that. Water temp. of Soyang river was very changable by month while that of the ground water in Suwon was kept constantly. In Soyang river of Chunchon the month that water temp. show $8{\sim}18^{\circ}C$, optimal growth temp., was May to Nov. and the month that water temp. show less than $6^{\circ}C$, growth limit temp., was $Jan.\;{\sim}\;Feb.$ of Chunchon. Rhizome weight of main stem in Chunchon and Suwon was 63g and 22g per plant and rate of maketable rhizome was each 80%, 0% by culture of 32 months to include raising see­dling period of 13 months. Dry matter partitioning ratio of petiole in Soyang river of Chunchon was the highest of all others but it was lowest of all others in ground water of Suwon. Rhizome weight of main stem in Chunchon was showed possitive correlation with plant height and fresh top weight and in Suwon it was showed possitive correlation with root weight and high possitive correlation with No. of total leaves and No. of tillers.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.27 no.5
• /
• pp.482-494
• /
• 1994

Most of carotenoprotein complexes have been extracted by using buffered solutions. However, in this study carotenoprotein from the muscle of Blue mussel(Mytilus edulis) was extracted by a detergent such as Triton X-100. It was purified and characterized by $20\%$ (w/v) $(NH_4)_2SO_4$, DEAE-cellulose ion exchange and Sephacryl S-300 gel filtration. The carotenoprotein(${\lambda}_{max}=462nm$) had an approximate M. W. of 372KDa(gel filtration). SDS-PAGE analysis of the carotenoprotein indicated the presence of four polypeptides of 60KDa($23.70\%$), 46.9KDa($9.14\%$), 26KDa($49.14\%$) and 13KDa($18.02\%$). Carotenoprotein denaturated by treatment with SDS to a final concentration of $0.2\%$ (w/v) caused a hypsochromic shift of ${\lambda}_{max}$ from 462nm to 456nm. The carotenoprotein contained lipids as structure units. The amino acid composition of the carotenoprotein contained large essential amino acid amounts of $62.8\%$, and the content of threonine($35.9\%$) was higher than other amino acids, but histidine, methionine and proline were not present. In the carotenoprotein, the major fatty acids were $C_{16:4},\;C_{16:0},\;C_{20:5}\;and\;C_{22:6}$. The percentages of polyunsaturated fatty acids($62.4\%$) were higher compared to other fatty acids(saturated fatty acids $19.6\%$, monounsaturated fatty acids $18.0\%$). Carotenoid was extracted from the carotenoprotein by acetone and it was separated into five different components by preparative TLC(benzene:petroleum ether:acetone=69:17:14). The major components of carotenoid were mytiloxanthin($74.79\%$) and 3,4,3'- trihydroxy-7',8'-didehydro-${\beta}$-carotene($18.26\%$), and they were at least presented as prosthetic groups of carotenoprotein.

• Journal of Bio-Environment Control
• /
• v.27 no.3
• /
• pp.253-259
• /
• 2018

This study set out to select a system to realize an optimal environment for strawberry cultivation greenhouses based on data about the growth and development of strawberry and its environment and to provide basic data for the research of its improved productivity. For these purposes, the investigator conducted a field survey with greenhouses for strawberry cultivation in western Gyeongnam. The findings show that farmers in their fifties and sixties accounted for the biggest part in the age groups of strawberry farmers. While those who were under 50 were accounted for approximately 67.5%, those who were 60 or older accounted for 32.5%. As for cultivation experiences, the majority of the farmers had ten years of cultivation experiences or less with some having 30 years of cultivation experiences or more. All the farmers built an arch type single span greenhouse. Those who used nutrient solutions were about 75.0%, being more than those who used soil. All of the farmers that used a nutrient solution adopted an elevated hydroponic system. The single span greenhouses were in the range of 7.5~8.5m, 1.3~1.8m and 2.5~3.5m for width, eaves, and ridge height, respectively, regardless of survey areas. The rafters interval was about 0.7~0.8m. In elevated hydroponic cultivation, the width, height, and interval of the beds were about 0.25m, 1.2m and 1.0m, respectively. As for the strawberry varieties, the domestic ones accounted for approximately 97.5% with Seolhyang being the most favorite one at about 65.0%. As for the internal environment factors of greenhouses, 38 farmers measured only temperature and relatively humidity. As for hydroponics, the farmers used a hydroponics control system. Except for the farmers that introduced a smart farm system for temperature and humidity control, approximately 85.0% controlled temperature and humidity only with a control panel for side windows and ventilation fans. As for heating and heat insulation, all of the farmers were using water curtains with many farmers using an oil or electric boiler, radiating lamp or non-woven fabric, as well, when necessary.

• Journal of the Korean Society of Marine Environment & Safety
• /
• v.21 no.5
• /
• pp.583-590
• /
• 2015

Submarine cable installation is essentials for grid connection between existing power grid and newly produced electricity which will be from offshore wind farm in Southwest sea area of Korea. Especially, submarine cable route and protection method is designed in order to ensure the economical efficiency, workability and stability of submarine cable installation. On this paper, we will give the basic information about the submarine cable route and protection method of offshore wind farm which will be built in Southwest sea area of Korea. For this, we have a numerical simulation at high and low tide based on the third-generation wave model SWAN(Simulating WAves Nearshore) using the long term wave data from Korea Institute of Ocean Science and Technology(KIOST). The results of the study, year mean Hs is 1.03m, Tz is 4.47s and dominant wave direction is NW and SSW When the incident wave direction is NW(Hs: 7.0 m, Tp: 11.76s), the distribution of shallow water design wave height Hs was calculated about 4.0~5.0m at high tide and 2.0~3.0m at low tide. When the incident wave direction is SSW(Hs: 5.84 m, Tp: 11.15s), the distribution of shallow water design wave height Hs was calculated about 3.5~4.5m at high tide and 1.5~2.5m at low tide. The wave direction on a dominant influence in the section of longitude UTM 249749~251349(about 1.6 km) and UTM 251549~267749(about 16.2 km) in the submarine cable route are each NW and SSW. Prominently, wave focusing phenomenon appears between Wi-do and Hawangdeung-do, in this sea area is showing a relatively high wave hight than the surrounding sea areas.

• Journal of Life Science
• /
• v.21 no.9
• /
• pp.1251-1258
• /
• 2011

Multiparous Hanwoo cull cows have been generally regarded to have poor meat quality compared to young and primiparous Hanwoo in Korea, and there have been few studies attempting to understand various feeding programs for the multiparous Hanwoo cull cow. In this study, the effects of a feeding program consisting of two different diets for multiparous Hanwoo cull cows on meat production and quality were tested in comparison to a commercially used diet. Diets for treatment consisted of two levels of crude protein contents, 14.28% and 12.70% for early fattening and finishing, respectively. For a control, commercially used fattening feed (12.39% crude protein) was used. Feeding trials were performed at three different farms. In farm A, 29 herds of multiparous Hanwoo cull cows were used for the treatment group and 3 herds for the control. In farms B and C, the number of animal herds for treatment and control were 8 vs. 3 and 11 vs. 4 herds, respectively. Experiment diets were fed for an average of 211 days and in treatment, early fattening diet was fed for 4 months and then finishing feed was fed until slaughter. Average daily gains, thickness of back fat, area of Longissimus dorsi, carcass weight, index of carcass weight, intramuscular fat, meat color and texture were analyzed after slaughter. Random effect model [8] was employed in effect analysis. Positive effects of treatment were found in terms of average daily gain, back fat thickness, Longissimus dorsi area, carcass weight, and intramuscular fat. Carcass yield index, meat color and texture showed a negative effect. In this study, significant results were not found in all factors analyzed because the variance between experiment farms was large, however the 90% confidence interval of summary effects of ADG, back fat thickness, Longissimus dorsi area and carcass weight were significant and that of carcass yield index, intramuscular fat, meat color and texture were less so. Conclusively, a high protein diet fed early during the fattening period in multiparous Hanwoo cull cows could have positive effects on meat production.