• Title/Summary/Keyword: 전구식

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Hyperproduction of L-Threonine by Adding Sodium Citrate as Carbon Source in Transformed Escherichia coli Mutant. (형질전환된 Escherichia coli변이주에서 Sodium citrate를 이용한 고농도 L-Threonine 생산)

  • 이만효;김병진;정월규;최선욱;박해룡;황용일
    • Journal of Life Science
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    • v.14 no.5
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    • pp.868-873
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    • 2004
  • The efficient fermentative production of L-threonine fermentation was achieved by using Escherichia coli MT201, transformed a plasmid carrying pyruvate carboxylase gene. It is an attempt to supply oxaloacetate to the L-threonine biosynthetic pathway. In order to improve the L-threonine productivity of E. coli MT201, a plasmid pPYC which is an expression vector of the pyruvate carboxylase gene of Coryne-bacterium glutamicum, was introduced. When E. coli MT/pPYC was incubated with medium containing only glucose as a carbon source, both the cell growth and L-threonine production were reduced, compared to the results from fermentation of E. coli MT201. In order to circumvent this effect, we attempted the addition of a mixed carbon source, composed of glucose and sodium citrate at a ratio of 1.5:3.5. It was shown that L-threonine production and cell growth (OD660) with E. coli MT/pPYC reached up to 75.7 g/l and 48, respectively, at incubation for 75 hr under fed-batch fermentation conditions. It is assumed that overproduction of L-threonine by anaplerotic pathway leads unbalance of TCA cycle and sodium citrate might playa role to recover normal TCA cycle.

Applicability of Partial Post-Tension Method for Deflection Control of Reinforced Concrete Slabs (RC슬래브의 처짐제어를 위한 상향긴장식 부분PT공법의 적용)

  • Lee, Deuck-Hang;Kim, Kang-Su;Kim, Sang-Sik;Kim, Yong-Nam;Lim, Joo-Hyuk
    • Journal of the Korea Concrete Institute
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    • v.21 no.3
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    • pp.347-358
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    • 2009
  • Recently, it is getting into a good situation for the flat-plate slab system to be applied. The flat-plate slab without beam, however, is often too weak to control deflection properly compared to other typical slab-beam structures, for which the post-tension method is generally regarded as one of best solutions. The post-tension (PT) method can effectively control deflection without increase of slab thickness. Despite this good advantage, however, the application of PT method has been very limited due to cost increase, technical problems, and lack of experiences. Therefore, in order to reduce difficulties on applying full PT method under the current domestic circumstances and to enhance constructability of PT system, this research proposed the partial PT method with top jacking anchorage applied in a part of span as need. For the top jacking anchorage system, the efficiency of deflection control shall be considered in detail because it can vary widely depending on the location of anchorage that can be placed anywhere as need, and tensile stresses induced at back of the anchorage zone also shall be examined. Therefore, in this study, analysis were performed on the efficiency of deflection control depending on the location of anchorage and on tensile stresses or forces using finite element method and strut and tie model in the proposed top jacking anchorage system. The proposed jacking system were also applied to the floor slabs at a construction site to investigate its applicability and the analysis results of slab behavior were compared to the measured values obtained from the PT slab constructed by the partial PT method. The result of this study indicates that the partial PT method can be very efficiently applied with little cost increase to control deflection and tensile stresses in the region as a need basis where problem exists.

Control of the Fruit-Piercing moths (과실 흡수나방의 방제효과)

  • Yoon Ju-Kyung;Kim Kwang-Soo
    • Korean journal of applied entomology
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    • v.16 no.2 s.31
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    • pp.127-131
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    • 1977
  • This experiment was conducted to evaluate the insect-proof netting, chemical sprays, application of attractants, fruit bagging and light trapping as the control methods of the fruit piercing moths in the orchards on reclaimed land in Sugyeri, Goksung, Chonnam Province, during June to October in 1976. The results are summarized as follows; 1. Insect-proof. netting effectively decreased fruit damage, compared as to the control, down to $9.4\%$ from $38.3\%$ in plum, $2.5\%$ from $53.0\%$ in peaches and $10.0\%$ from $29.0\%$ in grapes. 2. The control effects of chemicals varied significantly among the 7 insecticides tested: Deoclean, Naphthalene, and Thiometon were more effective to the fruit damages as low as $2.0\%,\; 3.6\%,\;and\;5.9\%$ respectively. while the fruit damage was rather high, $9.8\%$ for Demeton, $10.1\%$, for Takju +lead arsenate and $14.2\%$ for Padan. ,3. In the test with 7 attractants, the largest number of moths attracted and killed was 416.by Takju+brown sugar and the next was 307 by Takju+venegor while this number was 141 by mixed solution (see text) which is rather lower than expectation The fruit damage was lowest in Takju+honey and$5.2\%$, the next was $5.60\%$ for Takju+venegor and the highest was $12.0\%$, Takju alone. 4. Fruit bagging with polyethylene film effectively decreased the fruit damage from the inserts but brought about severe fruit rot and delay ripening. Meanwhile, paper bagging was less effective in preventing insects, resulting in $17.5\%$ fruit damage, however, gave no adverse effect other than slight Belay in ripening. 5. Light trapping was hardly expected to be a method of controlling these fruit piercing moths. However, the number of collected moths swarmed by electric light was 10.8 for can-descence, 0.95 for blue, and 0.22 for yellow light.

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GPR Survey for the Assessment of Scour Depth near Bridge Piers (세굴심 추정을 위한 교각 우물통 주변의 GPR 탐사)

  • Park, In-Chan;Cho, Won-Cheol;Lee, Chong-Guk
    • Proceedings of the Korea Water Resources Association Conference
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    • 2005.05b
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    • pp.996-1000
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    • 2005
  • 본 연구에서는 마포대교 확장 공사의 인해 일부 교각의 우물통 기초 주변 하상변화와 세굴 현상을 파악하기 위한 심도추정 방법으로 현재 다양하게 토목분야에서 적용되고 있는 치하투과레이더 탐사기법을 사용하였다. 교각 우물통 주변의 안정적인 GPR 탐사를 수행하기 위한 보조장비를 고안하여 마포대교 P6의 우물통 주변을 2004년 6월(1차)과 2004년 10월(2차)에 걸쳐 탐사를 실시하였으며, 탐사 기간 중 약 12,000 cms의 유출량이 발생하여 국부세굴과 하상 변화 가능성을 충분히 검토 할 수 있을 것으로 판단되었다. 수집된 자료를 통해 양질의 자료 획득을 위해서 자료의 보정 과정을 마친 후 각각의 구간에 대해서 1, 2차 탐사된 두 개의 자료를 비교 분석하였다. 전구간의 반사파를 이용하여 기존의 연구결과에서 얻어진 반사파의 양상(Beres and Haeni, 1991)과 비교한 결과 하상 표면은 미사 혹은 실트로 구성되어 있고 주요한 하상 하부 매질은 전석(boulder), 호박돌 등으로 이루어져 있었다. 그리고 1차 및 2차 탐사 자료와 DATAPCS의 세굴센서가 설치된 측점을 기준으로 심도 및 위치 보정을 한 결과 큰 오차는 발생하지 않았으며, 400 MHz 안테나를 활용하여 최대수심 약 10 m 이상 하상을 탐사할 수 있었다. 수심이 깊은 구간은 하상과 가까운 위치에서 탐사를 실시하여 양질의 하상 변화 양상을 파악할 수 있어 하상 재료의 정성적인 평가를 높일 수 있도록 하였다. 마포대교의 탐사 대상 교각 우물통 주변은 계획홍수량의 1/3 수준으로 비교적 적은 홍수량이 발생한 2004년의 호우사상으로 인해 일부구간이 약 $10\~20\;cm$ 정도의 퇴적과 세굴 영향이 나타난 것을 제외하고는 유의할 만한 세굴과 하상 변화가 발생하지 않고 매우 안정적인 상태를 보이고 있으며 되메움이나 두드러진 퇴적층의 양상 또한 확인할 수 없었다. 대상구간에 설치한 유속계 최대유속이 2.0 m/s 이상 발생하였지만 우물통 주변의 자갈 및 호박돌 등과 같은 평균입경이 큰 유사의 이동에 절대적인 한계유속을 초과하지 못한 것으로 판단된다. GPR 탐사의 적용 한계성을 극복한 본 연구는 홍수 전$\cdot$후의 하상변화 및 최대세굴심, 되메움 깊이 및 범위 등의 세굴현상을 현장탐사를 바탕으로 현재 다양하게 적용하고 있는 세굴 실험식과 비교 분석함으로써 우물통 주변의 효율적인 세굴 보호 대책을 강구하는 목적으로 그 활용성을 증대할 수 있을 것이다.

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Gold Colored Coating of TiO2 thin film on Nickel-Silver by Sol-Gel Process (양백에 코팅된 비정질 TiO2 박막의 특성에 관한 연구)

  • Lim, Yongmu;Kim, Sangmoon;Shim, Moonsik;Jang, Heejin;Shin, Jongyoon;Hwang, Kyuseog
    • Journal of Korean Ophthalmic Optics Society
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    • v.4 no.1
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    • pp.51-56
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    • 1999
  • $TiO_2$ thin films were spin coated on commercial Nickel-Silver with 2%Ti-naphthanate toluene solution by sol-gel process. The thin films were heat-treated at $500^{\circ}C$ for 2min and then decorative-protective coating was obtained. The conditions for gold colored coating, and the morphology, microstructure and color of the coating surface were have been characterized by Optical microscope, Photospectrometer and XRD. The heating temperature of colored-$TiO_2$ coating was $500^{\circ}C$ and was preferable. The average thickness of 1 time coating was $0.24{\mu}m$ and the total thickness increased linearly as a function of the coating time. The color of amorphous colored-$TiO_2$ coating was changed from gold to dark redish gold with coating time. 'L' as lightness and 'b' were decreased to red, but 'a' was decreased to blue after increase with coating time.

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A Study on the Analysis for the Effects of the Section Speed Enforcement System at the Misiryeong tunnel section (구간속도위반 단속장비 설치효과 분석 - 미시령동서관통도로를 중심으로 -)

  • Lee, Ho-Won;Joo, Doo-Hwan;Hyun, Cheol-Seung;Jeong, Jun-Ha;Park, Boo-Hee;Lee, Choul-Ki
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.12 no.3
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    • pp.11-18
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    • 2013
  • Since 1996, Korean National Police Agency has been promoting a project for installation of Automated Speed Enforcement (ASE) system aiming at reduction of accidents. The number has increased to 5,348 stations throughout country as of December 2012. Recently, the Section Speed Enforcement Systems have been installed at many sites to produce a general effect well beyond the localised effect at overt fixed camera sites. In this study aims, we have analyzed the effects of the Section Speed Enforcement System at the Misiryeong tunnel section. We have found that there were a statistically significant 21.4%~31.% reduction of the average speed and 45.9% reduction in a number of traffic accidents per month. Accordingly, the study indicates that the Section Speed Enforcement Systems at Misiryeong tunnel section has effective to produce road safety.

A Study on the Applicability of Partial Post-Tension Slab with Top Anchorage System (상향긴장식 부분PT를 사용한 슬래브의 적용성 분석)

  • Lee, Deuck-Hang;Kim, Kang-Su;Kim, Chang-Hyuk;Kim, Sang-Sik;Kim, Yong-Nam;Chung, Kwang-Ryang
    • Proceedings of the Korea Concrete Institute Conference
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    • 2008.11a
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    • pp.309-312
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    • 2008
  • Reinforced concrete (RC) structures have been most widely used because of the economic efficiency. However, it is very weak to tensile stresses and difficult to control deflection due to the heavy self-weight of concrete. Although it is generally known that prestressed concrete structures can be the most effective to overcome the demerit of RC structures, its application is very seldom in domestic construction for the difficult onsite circumstances. The post-tension method, which is well fit for buildings that are mostly indeterminate structures and beneficial for monolithic construction, has been introduced to just a few building construction. The application of full PT method into entire spans makes construction engineers feel very difficult due to the lack of current condition in construction fields. Therefore, this study proposed the partially applied PT method as an alternative, which can improve the deflection control of RC structures and reduce the construction difficulty by applying the PT method in a part of span length as needed, and analyzed its characteristics of structural behavior. In this study, the top anchorage was applied to improve the applicability of partial PT method, and the analysis results of slab behavior were compared to the measured values obtained from the post-tensioned slab constructed by the partial PT method.

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Dental application of glass-ceramic materials for aesthetic restoration (심미수복을 위한 글라스-세라믹 재료의 치과 응용)

  • Bae, Tae Sung
    • The Journal of the Korean dental association
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    • v.58 no.7
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    • pp.435-442
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    • 2020
  • Porcelain is the first ceramic material to be introduced into dentistry. Porcelain jacket crown was introduced by Dr. Charles H Land in 1886, which was an excellent aesthetic dental restoration but has not been widely used due to high firing shrinkage and low tensile strength. Then metal-ceramic system, which combines the esthetic properties of ceramics and the mechanical properties of metals, was introduced and nowadays it is still used in dental clinical field. However, the metal-ceramic system has shown some problems, such as increased lightness by reflection of light at opaque layer, shadow beneath the gingival line due to the block-out of light by metal coping, exposure of metal in margin part, bond failure between metal and porcelain, oxidation of metal coping during firing the porcelain, etc. Recently, along with the advance of fabrication methods of dental ceramics, the all-ceramic restorations with high esthetic and mechanical properties has increased and gradually replaced metal-ceramic restorations. Especially, CAD/CAM technology has opened a new era in fabricating the dental ceramic restorations. This overview will take a look at the past, present and future possibility of the dental ceramic materials.

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The Studies on synthesis of $SnO_2$ doped $In_2O_3$ (ITO: Indium Tin Oxide) powder by spray pyrolysis (분무열분해법(Spray Pyrolysis)에 의한 주석산화물이 도핑된 $In_2O_3$(ITO: Indium Tin Oxide)의 분말 제조에 대한 연구)

  • Kim, Sang Hern
    • Journal of the Korean Applied Science and Technology
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    • v.31 no.4
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    • pp.694-702
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    • 2014
  • The micron-sized ITO(indium tin oxide) particles were prepared by spray pyrolysis from aqueous precursor solutions for indium, and tin and organic additives solution. Organic additives solution with citric acid(CA) and ethylene glycol(EG) were added to aqueous precursor solution for Indium and Tin. The obtained ITO particles prepared by spray pyrolysis from the aqueous solution without organic additives solution had spherical and filled morphologies whereas the obtained ITO particles with organic additives solution had more hollow and porous morphologies with increasing mole of organic additives. The micron-sized ITO particle with organic additives was changed fully to nano-sized ITO particle whereas the micron-sized ITO particle without organic additives was not changed fully to nano-sized ITO particle after post-treatment at $700^{\circ}C$ for 2 hours and wet-ball milling for 24 hours. The size of primary ITO particle by Debye-Scherrer formula and surface resistance of ITO pellet were measured.

Transmittance Characteristics of Fishing Lamp in the Anchovy Scoop Fishery (멸치초망어업용 집어등 광력의 수중 투과특성)

  • 박성욱;배봉성;안희춘;서두옥
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.37 no.2
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    • pp.117-123
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    • 2001
  • Anchovy scoop nets, a kind of lift net have been used in the coastal area of Cheju and South sea of Korea. An incandescent lamp(AC 100V, 1kW) is being used as a fishing lamp for gathering anchovy. Fishing lamp was installed at 1m ahead of the prow and 1.5m higher than the water surface. The fishing lamp let fish school rise to the water surface and attract to bag net. Accordingly, a successful anchovy catching depends on controling of fishing lamp. On the study, the distribution of spectral irradiance illumination of incandescent lamp(1kw) and the irradiance efficiency of reflection plate were analyzed and discussed to investigate the ability of fishing lamp which can attract anchovy school effectively. The results obtained are summarized as follows : 1. Around 180% of irradiance efficiency of incandescent lamp was increased by using the reflection plate. The light of lamp was radiated into water with circular shape. 2. The irradiance illumination of incandescent lamp in air was a maximum in wave length of 994nm but it was 690nm at 0.5m and 1.0m of water depth. 3. The relationship between water depth(x) and water illumination(y) of vertical light is represented as follows : y=146.03e supper(-0.37x) 4. The light of incandescent lamp(1kW) pass through much better into vertical direction than horizontal but it was estimated that the light was not able to reach depth of 20m.

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