• 한국생물공학회:학술대회논문집
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• 2005.04a
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• pp.541-543
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• 2005

Recently many efforts have been made to develop new therapeutic agents against skin pigmentation abnormalities. The aim of this study is to investigate the skin-whitening effect of Gleditsia Sinensis Lam and to develop new drug delivery carrier. Inhibitory effaces of Gleditsia Sinensis Lam extracts on melanin synthesis were studied. Namely, UV-absorbing ability, free radical scavenging activity and tyrosinase inhibitory of Gleditsia Sinensis Lam extracts were investigated As a result, the extracts of Gleditsia Sinensis Lam were found to inhibit the activity of tyrosinase. Moreover they absorbed wavelength in the UV-B and UV-C region, and extracts of Gleditsia Sinensis Lam had free radical scavenging activity. In this study, in order to administer these ingredient effectively, the shape of liposome observed by TEM was spherical and uniform.

• KSBB Journal
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• v.7 no.4
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• pp.302-307
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• 1992

Protein phosphatase 2A was obtained from a cytosolic fraction of bovine brain homogenate. The phosphatase activity using phosphorylated histone Hl as substrate was suppressed in the presence of liposomes composed of dipalmitoylphosphatidylcholine(DPPC) or the mixture of phosphatidylserine and DPPC. The binding of protein phosphatase to liposome was indicated by the facts that the phosphatase activity of the supernatant of protein phosphatase/multilayer vesicle mixture was decreased with increasing amount of liposome, and that [$^{125}I$]-labeled protein phosphatase was coeluted with liposome. However, the affinity of the protein for phospholipid membrane was not so high. On the other hand, okadaic acid and liposome reduced the phosphatase activity synergistically, which means that okadaic acid binds neither to lipid membrane nor to the membrane-associated phosphatase, The inhibitory effect of liposome was, therefore, ascribed to association of the protein phosphatase 2A with the lipid bilayer membrane.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.20 no.3
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• pp.98-106
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• 2007

포공영(蒲公英)은 예로부터 청열해독약(淸熱解毒藥)으로 사용되어 왔으며 NO가 염증의 한 요인이기 때문에 포공영의 항염증 작용기작을 밝히기 위하여 LPS로 자극된 대식세포주 RAW264.7 세포에서 포공영 열수 추출물 (AETM)의 NO 생성에 미치는 효과를 조사하였다. 포공영은 NO 생성 및 iNOS 단백질 발현, iNOS mRNA 발현을 저해하였으며, 전사인자인 $NF-kB$의 핵으로의 이동을 억제하였다. 또한 LPS에 의해서 활성화되는 ERK/MAPK 효소의 활성을 현저히 억제하였다. 이 결과들로 보아 포공영의 항염증 작용이 ERK/MAPK 활성 저해 및 $NF-kB$ 활성 저해로 인한 iNOS 발현의 억제 때문인 것으로 사료된다.

• Journal of Korean society of Dental Hygiene
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• v.15 no.4
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• pp.735-741
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• 2015

Objectives: Lentinus edodes is an edible mushroom with a variety of beneficial effects such as antitumor, anti-inflammatory, antioxidant, and immune-modulatory activity. This study was carried out to evaluate the antimicrobial activities of Lentinus edodes extracts against oral-related bacteria. Methods: The antimicrobial activities of this extracts were investigated against S. anginosus, S. sobrinus, S. aureus, S. mutans, S. ratti, S. sanguinis, A. viscosus, A. naeslundii, and A. actinomycetemcomitans by the disc diffusion method, minimum inhibitory concentration (MIC), and growth inhibition. Results: Ethanol extracts had no antimicrobial activities, but acetone extracts showed antimicrobial activities against A. viscosus and A. actinomycetemcomitans and ethyl acetate extracts had effects against S. aureus, S. sanguinis, A. viscosus, and A. actinomycetemcomitans. Conclusions: The inhibitory effect of Lentinus edodes extracts was investigated on the growth of A. viscosus. Ethyl acetate and acetone extracts showed 90% and 77% inhibitory effect, respectively, against A. viscosus for 24 hrs. Ethyl acetate extracts had MIC of 25.0 mg/ml and acetone extracts showed MIC of >25.0 mg/ml.

• Journal of Korean Society of Water and Wastewater
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• v.28 no.1
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• pp.47-54
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• 2014

Nanotechnology has become one of the fastest developing technologies and recently applied to a variety of industries. Thus, increasing number of nano materials including various nanoparticles would be discharged into wastewater and consequently entering a biological wastewater treatment process. However, the impact of the nano particles on biological wastewater treatment has not been estimated intensively. In this research, we investigated the effect of silica nanoparticle on the oxygen uptake rates (OURs) of activated sludge used in a conventional wastewater treatment process. The inhibition (%) values were estimated from the results of OURs experiments for the silica nanoparticles with various sizes of 10-15, 45-50, and 70-100 nm and concentrations of 50, 250, and 500 ppm. As results, the inhibition value was increased as the size of silica nano particles decreased and the injected concentration increased. The maximum inhibition value was investigated as 37.4 % for the silica nanoparticles with the size of 45-50 nm and concentration of 50 ppm. Additionally, the effect of size and concentration on the inhibition should be considered cautiously in case that the aggregation of particles occurred seriously so that the size of individual particles was increased in aquatic solution.

• Journal of Applied Biological Chemistry
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• v.64 no.2
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• pp.121-125
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• 2021

Antioxidative, anticoagulant, and α-glucosidase inhibitory effects of 60% (v/v) ethanolic extract from lotus (Nelumbo nucifera) leaves were investigated in vitro. The yield and polyphenol content of the lotus leaves extract were 35.3±1.1% and 186.2±5.2 ㎍ gallic acid equivalents/mg, respectively. The lotus leaves extract effectively scavenged free and cation radicals, and nitrite in a concentration-dependent manner, and possessed potent reducing power. The lotus leaves extract had remarkable anticoagulant and α-glucosidase inhibitory activities as a function of the extract concentration.

• 한국약용작물학회:학술대회논문집
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• 2007.05a
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• pp.183-184
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• 2007

• YAKHAK HOEJI
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• v.46 no.6
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• pp.375-380
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• 2002

In this study, newly designed COX-2 inhibitors, synthetic derivatives of benzothiazine-3-carboxamide, were screened in vitro for selectivity of COX-1 and COX-2 inhibition properties. 7-Bromo-1,2-benzoisothiazine derivatives were obtained from 4-bromotoluene over the chlorosulfonation, amination and oxidation. And benzothiazine ring was synthesized through Gabriel-Colmann rearrangement reaction. To evaluate inhibitory effect of COX-2, synthetic derivatives of benzothiazine-3-carboxamide were tested with accumulation of prostaglandin by lipopolysaccharide in aspirin-treated murine macropharge cell. Some of the synthesized lead compounds have potentially shown the structure-activity relationship for selectivity of COX-2 inhibition activity.

• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 2001.05a
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• pp.118-118
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• 2001

Lemna gibba에 대한 성장저해시험은 OECD Lemna Growth Inhibition Test(1998)와 EPA Auqatic Toxicity Test Using Lemna sp, (OPPTS 850.4400)에 제시된 시험 방법으로, 시험물질에 대한 수중식물의 독성값을 알기 위하여 광범위하게 사용되고 있다. 본 실험은 각각의 Guideline에서 제시된 endpoint를 서로 비교하여, 가장 효과적으로 시험의 결과를 표현 할 수 있는 endpoint를 찾고자 하였으며, 이때 사용된 endpoint로는 잎의 수, 잎 면적, 습중량, 건중량을 이용하였다. (중략)

• Proceedings of the Korean Society of Applied Pharmacology
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• 1993.04a
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• pp.128-128
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• 1993

총 40종 생약의 hyaluronidase에 대한 저해 효과를 검색한 결과, 백지, 오수유, 향부자, 섬백리향, 배풍등, 황금, 황련, 진피, 소리쟁이, 반디나물 등 10종 생약에서 저해 활성을 관찰할 수 있었다. 또한 권백의 n-BuOH 분획에서 2개의 biflavonoid 성분인 amentoflavone, isocryptomerin을 분리하였으며, chloroform 분획에서 cryptomerin B를 분리하였다. 이들 화합물의 화학구조는 각종 기기분석 data를 이용하여 결정하였으며, 이들 화합물중 cryptomerin B는 Selaginellaceae에서 처음으로 분리 보고되는 화합물이다. 현재 이들 화합물의 생리 활성에 대하여 검사중이다.