• Title/Summary/Keyword: 저항성 계통

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Breeding of a New Late-season Pear Cultivar 'Mansoo' with Large Sized High Quality and Long Storability (저장력 강한 고품질 대과 만생종 배 '색수(晩秀)' 육성)

  • Kim, Whee-Cheon;Hwang, Hae-Sung;Shin, Il-Sheob;Shin, Yong-Uk;Lee, Don-Kyun;Kang, Sang-Jo;Moon, Jong-Youl;Kim, Jung-Ho
    • Horticultural Science & Technology
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    • v.19 no.1
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    • pp.66-70
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    • 2001
  • 'Mansoo' is a late-season pear cultivar with extra large and high quality fruit of long storability, which was released by National Horticultural Research Institute in 1995. The cultivar originated from the cross between 'Danbae' and 'Okusankichi' at Suwon in 1978, had been under regional adaptability test at nine areas in the name of 'Wonkyo Na-15' for 4 years since 1992. It was vigorous in tree growth and upright in tree shape. Its flower bud is easily maintained, resulting in higher productivity than 'Danbae' and 'Okusankichi'. It bloomed a day later than 'Niitaka', and showed cross-compatibility with 'Niitaka', 'Chojuro' and some other varieties. Harvesting time of 'Mansoo' is late October in Suwon. The fruit shape is oblate and skin color is light yellowish brown. The fruit weighed 600-700 g and has 12-13% soluble solids content. The flesh is soft, juicy, and has negligible grit. It is resistant to black leaf rot (Alternaria kikuchiana Tanaka).

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Isolation and Characterization of Helicobacter pylori Urease Inhibitor from Rubus coreanus Miquel (복분자(Rubus coreanus Miquel)로부터 Helicobacter pylori Urease Inhibitor의 분리 및 특성)

  • 양성우;호진녕;이유현;신동훈;홍범식;조홍연
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.5
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    • pp.769-777
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    • 2004
  • A Helicobacter pylori urease inhibitor from Rubus coreanus Miquel has been isolated and partially characterized for aiming to Prevent H. pylori growth and decrease harmful accumulation of ammonia in human gastric mucosa. We screened urease inhibitory activities in 519 extracts library prepared by solvent extraction from 173 kinds of edible plants, medicinal herbs, herbs and seaweeds using a colorimetric urease assay system. As results of primary and secondary screening, 70% acetone extract of Rubus coreanus Miquel was selected as potent candidate, showing about 24% inhibitory activity. The acetone extract was sequentially partitioned into RCE/RCWI and RCB/RCW2 layers with ethyl acetate and butanol. The major active component in RCW2, water layer from butanol fractionation was revealed to be peptidic or proteinous substance by inhibitory activity determination after pronase digestion and periodate oxidation. RCW2-IIIc a was isolated by sequential column chromatography on DEAE-Toyopearl 650C, Butrl-Toyopearl 650M and Sephadex LH-20. The isolated urease inhibitor RCW2-IIIc $\alpha$, was highly pure proteinous substance with molecular weight of 13kDa by high-performance gel permeation liquid chromatography. RCW2-IIIc$\alpha$ has about 5 times higher inhibitory activity than 70% acetone extract, showing high stability against heat treatment and peptic digestion.

A New High-Quality, Disease Resistance and High-Yielding Rehmannia glutinosa Cultivar, "Kokang" (고품질 내병 다수성 지황 고강)

  • Kim, Dong-Hwi;Park, Chung-Heon;Park, Hee-Woon;Park, Chun-Geun;Sung, Jung-Sook;Yu, Hong-Seob;Kim, Geum-Soog;Seong, Nak-Sul;Kim, Jae-Chul;Kim, Myeong-Seok;Bae, Su-Gon;Chung, Byeong-Jun
    • Korean Journal of Breeding Science
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    • v.40 no.1
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    • pp.84-87
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    • 2008
  • Kokang is a high-quality, disease resistant and high-yielding Rehmannia glutinosa cultivar developed by the medicinal crop breeding team of National Institute of Crop Science, RDA, during the period from 1997 to 2005. The reproduction of Rehmania glutinosa has been accomplished mainly by vegetative propagation with its seedlings have many variants. The cultivar was selected from seedling of Jihwang 1. The plant type of Kokang is some rising from ground. It has higher disease resistance, catalpol content and extract content compared with Jihwang 1. Regional yield trials conducted at three site from 2003 to 2005. The root yield of Kokang was 11.8ton per hectare, which was increased 13% compared with a check variety, Jihwang 1. This cultivar is adaptable to the whole of Korea except for mountain areas.

Characteristic of mycelial growth of cauliflower mushroom (Sparassis latifolia) using replacement culture with Trichoderma and rDNA analysis in genealogy of crossbreeding strain (푸른곰팡이 대치배양에 의한 꽃송이버섯 균사 생장 특성 및 계통간 교잡균주의 rDNA 분석)

  • Oh, Deuk-Sil;Kim, Hyun-Suk;Kim, Young;Wi, An-Jin;Yoon, Byung-Sun;Park, Whoa-Shig;Park, Hyeong-Ho;Wang, Seung-Jin
    • Journal of Mushroom
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    • v.12 no.1
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    • pp.41-51
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    • 2014
  • Cauliflower mushroom widely known high concent of ${\beta}$-glucan for farm cultivation invigoration verified characteristics of mycelia growth, genetic diversity, resistance to Trichoderma by replacement culture with Trichoderma and growth characteristics of new variety crossbleeding strain. The result of replacement culture with Trichoderma for verification resistance about Trichoderma, 6951 (T. viride) strain did not show special change after formation of confrontation line and 6952 (T. spp.) strain was showed more formation of spore after formation of confrontation line. But 6426 (T. harzianum) strain found to encroach part of growth area of cauliflower mushroom mycelia. Among 10 kinds cauliflower mushroom strain, JF02-06 strain collected by Gurye, found did not spore of Trichoderma and thought to be resistant to Trichoderma. The result of crossbleeding after selected that mother strain good growth and formation of fruit body, verified good mycelia growth at JF02-47, 49 and 50 strain in Korean pine of wood-chip media. The result of gene sequence about ITS1, 5.8S and ITS4 for analysis of genetic diversity at crossbleeding strain, found high significance to other cauliflower mushroom in registered Genebank. The result of growth characteristic of spore and mycelia of cauliflower mushroom by observation microscope, size of spore showed water drop shape to major axis $6{\mu}m$ and minor axis $5{\mu}m$ and clamp showed 3 types in mycelia. The wide of mycelia was $3{\mu}m$. The characteristic of mycelia of cauliflower mushroom found to grow mycelia in clamp at approximately 50%. The growth speed of mycelia was $0.507{\mu}m/min$ and 2nd mycelia grown similar speed to mother mycelia at parallel with mother mycelia after growth speed at $0.082{\mu}m/min$. The formation of clamp made small clamp for 5 hours after shown transfer of electrolyte in mycelia inside. The septum formation started after 3 hours and then finally completed after 2 hours. In this study, strain of cauliflower mushroom verified resistance of Trichoderma, genetic diversity and characteristic of mycelia growth. Therefore, basic knowledge of cauliflower mushroom will improve and further contribute to development of mushroom industry.

Growth Characteristics and Productivity of Single Cross Maize New Hybrid for Silage and Grain, 'Yanganok' (사료용 옥수수 신품종 '양안옥'의 생육특성 및 수량성)

  • Son, Beom Young;Baek, Seong Bum;Kim, Jung Tae;Lee, Jin Seok;Ku, Ja Hwan;Kim, Sun Lim;Jung, Gun Ho;Kwon, Young Up;Ji, Hee Jung;Huh, Chang Suk;Park, Jong Yeol
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.33 no.2
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    • pp.94-99
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    • 2013
  • Yanganok, a new single cross variety, was developed by the maize breeding team at the National Institute of Crop Science (NICS), RDA in 2012. This hybrid, which has a high yield of grain was produced by crossing two inbred lines, KS164 and KS163. KS164 is the seed parent and KS163 is the pollen parent of Yanganok. It is a yellow-orange intermediate maize hybrid (Zea mays L.). After a preliminary yield trial and advanced yield trial of Yanganok (Suwon180) in Suwon for 2 years, a regional yield trial was carried out for its growth characteristics, and yields were determined at 4 different locations from 2009 to 2012, with the exception of 2010. It was named 'Yanganok'. The silking date of Yanganok is 2 days earlier than that of the check hybrid, Kwangpyeongok. Plant height of Yanganok is similar to Kwangpyeongok, but its ear height ratio is higher than that of Kwangpyeongok. It has resistance to lodging. Stay-green features of Yanganok are similar to those of Kwangpyeongok. The ear ratio of Yanganok is similar to Kwangpyeongok. It has moderate resistance to southern leaf blight (Bipolaris maydis) and strong resistance to northern leaf blight (Exserohilum turcicum). It has moderate resistance to black streaked dwarf virus (BSDV), ear lot and corn borer. The dry matter yield of Yanganok, 17.45 ton/ha, was similar to that of Kwangpyeongok. The total digestible nutrients (TDN) yield of Yanganok, 11.96 ton/ha, was similar to that of Kwangpyeongok. The grain yield of Yanganok, 8.32 ton/ha was similar to that of Jangdaok. Seed production of Yanganok went well due to a good match during crossing between the seed parent, KS164, and the pollen parent, KS163, in Yeongwol, and F1 seed yield was 1.79 ton/ha. Yanganok would be a suitable cultivar to all plain areas in the Korean peninsula.

Growth Characteristics and Productivity of New Single Cross Maize Hybrid for Grain, 'Singwangok' (종실용 옥수수 신품종 '신광옥'의 생육특성과 수량성)

  • Son, Beom Young;Baek, Seong Bum;Kim, Jung Tae;Lee, Jin Seok;Hwang, Jong Jin;Kim, Sun Lim;Jung, Gun Ho;Kwon, Young Up;Huh, Chang Suk;Park, Jong Yeol
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.34 no.1
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    • pp.21-25
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    • 2014
  • Singwangok, a new single cross variety, was developed by the maize breeding team at the National Institute of Crop Science (NICS), RDA in 2012. This hybrid, consisting of a high yield of grain, was produced by crossing two inbred lines, KS172 and KS173. KS172 is the seed parent and KS173 is the pollen parent of Singwangok. It is a yellow-orange intermediate maize hybrid (Zea mays L.). After the preliminary yield trial and advanced yield trial of Singwangok (Suwon185) in Suwon for 2 years, the regional yield trial of that was subsequently carried out for its growth characteristics and yield at 3 different locations from 2010 to 2012. It was named as Singwangok. The silking date of Singwangok is similar to the check hybrid, Jangdaok. The plant height of Singwangok is similar to Jangdaok, but its ear height ratio is lower than that of Jangdaok. Moreover, it has a resistance to lodging. The ear number per 100 plants of Singwangok is more 17 than that of Jangdaok, whereas the ear length of Singwangok is similar to Jangdaok. Further, the weight of 100 seeds of Singwangok is similar to Jangdaok. It has a moderate resistance to southern leaf blight (Bipolaris maydis) and a strong resistance to northern leaf blight (Exserohilum turcicum). Furthermore, it has a moderate resistance to the black streaked dwarf virus (BSDV), ear lot and corn borer. The grain yield of Singwangok, 7.81 ton/ha, was similar to that of Jangdaok. The seed production of Singwangok was well processed due to the good match during crossing between the seed parent, KS172 and the pollen parent, KS173, in Yeongwol; the F1 seed yield was 3.84ton/ha. Singwangok would be a suitable cultivar to all plain areas in Korea.

Development of a High Heat Load Test Facility KoHLT-1 for a Testing of Nuclear Fusion Reactor Components (핵융합로부품 시험을 위한 고열부하 시험시설 KoHLT-1 구축)

  • Bae, Young-Dug;Kim, Suk-Kwon;Lee, Dong-Won;Shin, Hee-Yun;Hong, Bong-Guen
    • Journal of the Korean Vacuum Society
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    • v.18 no.4
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    • pp.318-330
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    • 2009
  • A high heat flux test facility using a graphite heating panel was constructed and is presently in operation at Korea Atomic Energy Research Institute, which is called KoHLT-1. Its major purpose is to carry out a thermal cycle test to verify the integrity of a HIP (hot isostatic pressing) bonded Be mockups which were fabricated for developing HIP joining technology to bond different metals, i.e., Be-to-CuCrZr and CuCrZr-to-SS316L, for the ITER (International Thermonuclear Experimental Reactor) first wall. The KoHLT-1 consists of a graphite heating panel, a box-type test chamber with water-cooling jackets, an electrical DC power supply, a water-cooling system, an evacuation system, an He gas system, and some diagnostics, which are equipped in an authorized laboratory with a special ventilation system for the Be treatment. The graphite heater is placed between two mockups, and the gap distance between the heater and the mockup is adjusted to $2{\sim}3\;mm$. We designed and fabricated several graphite heating panels to have various heating areas depending on the tested mockups, and to have the electrical resistances of $0.2{\sim}0.5$ ohms during high temperature operation. The heater is connected to an electrical DC power supply of 100 V/400 A. The heat flux is easily controlled by the pre-programmed control system which consists of a personal computer and a multi function module. The heat fluxes on the two mockups are deduced from the flow rate and the coolant inlet/out temperatures by a calorimetric method. We have carried out the thermal cycle tests of various Be mockups, and the reliability of the KoHLT-1 for long time operation at a high heat flux was verified, and its broad applicability is promising.

Growth Characteristics and Productivity of Single Cross Maize Hybrid for Grain, 'Andaok' (다수성 종실 옥수수 신품종 '안다옥'의 생육특성과 수량성)

  • Son, Beom Young;Baek, Seong Bum;Kim, Jung Tae;Lee, Jin Seok;Ku, Ja Hwan;Kwon, Young Up;Huh, Chang Suk;Park, Jong Yeol
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.33 no.1
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    • pp.1-5
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    • 2013
  • Andaok, a new single cross variety, is a yellow dent maize hybrid (Zea mays L.) developed by the maize breeding team at the National Institute of Crop Science (NICS), RDA in 2011. This hybrid, which has a high yield of grain, was produced by crossing two inbred lines, KS161 and KS162. KS161 is the seed parent and KS162 is the pollen parent of Andaok. Silking date of Andaok is 3 days later than that of the check hybrid, Jangdaok. Plant height of Andaok is longer than that of Jangdaok. Ear numbers per 100 plants of Andaok is more than that of Jangdaok. Ear length of Andaok is shorter than that of Jangdaok. The weight of 100 seeds of Andaok is heavier than that of Jangdaok. It has moderate resistance to southern corn leaf blight (Bipolaris maydis), black streaked dwarf virus (BSDV) and corn borer. It has strong resistance to northern corn leaf blight (Exserohilum turcicum). It has resistance to lodging. Andaok was evaluated for its yield of grain at three locations from 2009 to 2011. The grain yield of Andaok was 7.80 ton/ha. Seed production of Andaok has gone well due to a good match during crossing between the seed parent, KS161, and the pollen parent, KS162, in Yeongwol.

Isolation of Bacillus licheniformis Producing Antimicrobial Agents against Bacillus cereus and Its Properties (Bacillus cereus 증식 억제능을 가지는 Bacillus licheniformis SCK 121057의 분리 및 특징)

  • Kim, Yong-Sang;Yun, Suk-Hyun;Jeong, Do-Yeon;Hahn, Kum-Su;Uhm, Tai-Boong
    • Korean Journal of Microbiology
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    • v.46 no.3
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    • pp.270-277
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    • 2010
  • In order to manufacture Bacillus cereus-free fermented soybean products, an antimicrobial agentproducing isolate against B. cereus was obtained from 150 traditionally fermented soybean products. The morphological and biochemical tests and the phylogenetic relationship among 16S rRNA gene sequences indicated that the isolate named as the strain SCK 121057 was most closely related to Bacillus licheniformis. The B. licheniformis isolate began to produce the antimicrobial agent after 48 h of incubation. The agent was nonproteinaceous and insensitive to heat, long term storage and protease K. Electron microscopic observation indicated that the agent attacked the membrane of B. cereus, leaving the ghost cell. The isolate inhibited growth of B. subtilis, Lactobacillus brevis and various types of pathogenic strains including Escherichia coli, E. faecalis, Micrococcus luteus, Staphylococcus aureus, Aspergillus flavus, A. ochraceus, and A. parasiticus as well as B. cereus. After coinoculation of B. licheniformis SCK 121057 and B. cereus in the ratio (as the basis of CFU/g sample) of 10 to 1 on the surface of cooked soybeans, cell numbers of B. cereus had been dramatically reduced after 31 days of incubation compared to those of single inoculation of B. cereus.

Different Responses of Zoysiagrass (Zoysia spp.) Ecotypes against Puccinia zoysiae Causing Rust Disease in Field (포장에서 녹병 병원균인 Puccinia zoysiae에 대한 한국잔디의 종별 상이한 반응)

  • Sung, Chang Hyun;Lee, Jeong Ho;Koo, Jun Hak;Hong, Jeum Kyu;Youn, Jeong Ho;Chang, Seog Won
    • Weed & Turfgrass Science
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    • v.5 no.4
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    • pp.256-259
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    • 2016
  • An obligate plant pathogenic fungus Puccinia zoysiae Dietel, the causal agent of rust disease, which is implicated in the damage of zoysiagrass (Zoysia spp.) in Korea. The fungus is one of the important pathogens, which attacks and colonizes susceptible zoysiagrass. Zoysia rust disease was observed in Jangseong, Hoengseong and Hapcheon in 2014 and 2015, Korea. The typical symptoms of the disease appeared first as small white spots on the leaf and stem of zoysiagrass plants, and turn brownish, dark brown spores revealed on the lesions. Uredospores were light brown in color, ellipsoid or spherical in shape and $22.0{\sim}25.0{\times}15.0{\sim}17.5{\mu}m$ in size. On the basis of the morphological characteristics, the fungus was identified as P. zoysiae. Pathogenicity of the fungus was proved by artificial inoculation on Z. japonica. The rust disease firstly appeared approx. early or middle June and then increased middle of October in Korea. In field, Z. matrella ecotypes were more resistant than Z. japonica ecotypes against the pathogen. Disease response against the pathogen was different among ecotypes of each species, respectively.